AMB Express
·
2018
·Vol. 8
·期 1
·1-8
·
IF 1.719
Liang Xiao-Sheng,Liu Chun,Long Zhu,Guo Xiao-Hua
Spore counting in probiotic Bacillus cultures using dipicolinic acid (DPA) as a marker was studied for developing a rapid and simple detection method. The newly developed method is based on the fluorescence enhancement by a new chelating agent, which forms a complex with EuCl 3 and DPA. The results showed that 1,2-cyclohexanediamine-N,N,N′N′-tetraacetic acid (CyDTA) greatly enhanced the fluorescence intensity in all selected chelating agents. The optimal composition of the fluorescence complex DPA-Eu-CyDTA had a detection limit of 0.3 nM of DPA. Metal ions in high concentrations, including Cu 2+ , Fe 2+ , Fe 3+ , Al 3+ , and Zn 2+ , might lower the detection sensitivity, which could be eliminated by diluting the sample with the metal ions below 10 μM. The maximum release of DPA was achieved by heating treatments at 121 °C for at least 10 min for two types of Bacillus endospores. The spore concentrations and corresponding released DPA fluorescence intensities were linearly associated (coefficient R 2 = 0.9993 and 0.9995 for Bacillus subtilis MA139 and Bacillus licheniformis BL20386, respectively). The detection limit for both strains reached approximately 6800 spores/mL. The verification results showed that the DPA fluorimetry assay developed in the present study was fully consistent with the plate-counting assay. The study shows that the fluorescence complex DPA-Eu-CyDTA can be reliably used for the detection of endospores in Bacillus fermentation for the production of probiotics.
研究领域:
微生物学
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生物技术
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食品科学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
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2015
·
IF 3.082
Lei Rao,Zhenzhen Xu,Yongtao Wang,Feng Zhao,Xiaosong Hu,Xiaojun Liao
The objective of this study was to investigate the inactivation of the Bacillus subtilis spores by high pressure CO 2 combined with high temperature (HPCD + HT) and to analyze the clumping effect of the spores on their HPCD + HT resistance. The spores of B . subtilis were subjected to heat at 0.1 MPa and HPCD at 6.5–25 MPa, and 82 °C, 86 °C, and 91 °C for 0–120 min. The spores were effectively inactivated by HPCD + HT, but a protective effect on the spores was also found, which was closely correlated to the pressure, temperature and time. The spores treated by HPCD + HT at 6.5 and 10 MPa exhibited a two-stage inactivation curve of shoulder and log-linear regions whereas the spores at 15–25 MPa exhibited a three-stage inactivation curve of shoulder, log-linear and tailing regions, and these curves were well fitted to the Geeraerd model. Approximately 90% of pyridine-2,6-dicarboxylic acid (DPA) was released after HPCD + HT and the 90% DPA release time depend on the pressure and temperature. Moreover, the spore clumping in suspensions was examined by dynamic light scattering. The particle size of the spore suspensions increased with the increase of pressure, temperature and time, indicating the spore clumping. 0.1% Tween 80 as a surfactant inhibited the spore clumping and increased the inactivation ratio of the spores by HPCD + HT. These results indicated that the spore clumping enhanced the spores' resistance to HPCD + HT and induced a protective effect.
研究领域:
微生物学
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食品科学
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DOI
PubMed
高影响因子
Environmental Technology & Innovation
·
2025
·
IF 7.1
Haixu Zhang,Sen Yang,Xilu Zhang,Yaxin Pei,Mengxiao Chen,Hongge Chen,Jiran Zhang
Cyromazine, a widely used insect growth regulator, accumulates in livestock manure and poses risks to beneficial insects such as black soldier fly larvae (BSFL) during waste bioconversion. This study isolated two bacterial strains capable of degrading cyromazine, Bacillus subtilis CYR1 (CYR1) and Bacillus velezensis CYR2 (CYR2), from contaminated chicken manure. Both strains demonstrated significant degradation efficiency via hydrolysis of cyromazine to cyanuric acid, a less toxic metabolite. However, in chicken manure systems, degradation efficiency reached a plateau at approximately 20%, likely due to preferential utilization of alternative nitrogen sources and inherent metabolic constraints. These findings highlight the potential of microbial remediation to mitigate cyromazine contamination while underscoring the need for strain optimization and comprehensive ecotoxicological assessments.
研究领域:
环境科学
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微生物学
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昆虫学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2024
·
IF 5
Yingying Sun,Lele Shao,Yana Liu,Bo Zou,Han Wang,Xingmin Li,Ruitong Dai
Bacillus cereus spores pose a significant concern during food processing due to their high resistance to environmental stress. Ohmic heating (OH) is an emerging and alternative heating technology with potential for inactivating such spores. This study evaluated the inactivation effects and the biological property changes of Bacillus cereus spores during OH treatments. OH effectively inactivated spores in milk, orange juice, broth, rice soup, and buffer solution in less time than oil bath heating (OB). A decrease in NaCl content improved spore inactivation at the same temperature. Spores were more sensitive to acid at 80–85 °C with OH treatment. Furthermore, OH at 10 V/cm and 50 Hz could reduce the spore resistance and inhibit an increase in spore hydrophobicity and spore aggregation. Both heating methods resulted in significant dipicolinic acid (DPA) leakage and damage to the cortex and inner membranes of the spores. However, OH at 10 V/cm and 50 Hz had the lowest DPA leakage and inflicted the least damage to the inner membrane. The damage to the spore's inner membrane was considered the primary reason for inactivation by OB and OH treatments. Still, OH at 10 V/cm and 50 Hz might also block the germination or outgrowth of treated spores or cause damage to the spore core.
研究领域:
食品科学
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微生物学
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生物技术
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产品关联
DOI
PubMed
高影响因子
Agriculture-Basel
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2024
·Vol. 14
·期 6
·881
·
IF 3.3
Ximei Xu,Xiaofeng Yue,Du Wang,Mengxue Fang,Li Yu,Fei Ma,Nanri Yin,Xuefang Wang,Baocheng Xu,Liangxiao Zhang,Peiwu Li
Cadmium is the main heavy metal contaminant of food in the world. The extent of cadmium pollution in peanut in China remains unclear. To determine the cadmium pollution level in peanut, samples from the main producing regions in China were assessed. The findings revealed that the cadmium pollution level in Chinese peanuts was relatively low. Moreover, the Aflatoxin Rhizobia CoupleB. amyloliquefaciens,B. laterosporu,B. mucilaginosus,E. ludwiggi(ARC-BBBE) microbial inoculants on cadmium contamination in peanut were evaluated. The fertilization methods were categorized into conventional fertilization and conventional fertilization supplemented with 60 kg/hectare of microbial inoculant ARC-BBBE as the base fertilizer. The cadmium contents in the soil and peanut plant parts were detected and analyzed. The results demonstrated that the microbial inoculant ARC-BBBE significantly reduced the total cadmium content in peanut, as well as the available cadmium and exchangeable cadmium in soil. Furthermore, the pH and urease and alkaline phosphatase activities in soil were significantly enhanced, suggesting that the microbial inoculant ARC-BBBE decreased cadmium content in soil and reduced the cadmium uptake by plants through a combination of the action of the bacteria itself and the secretion of extracellular substances. This ultimately achieves the goal of reducing the cadmium content in peanut seeds.
研究领域:
环境科学
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农业
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微生物学
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土壤学
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植物学
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DOI
高影响因子
FOOD CHEMISTRY
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2022
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IF 9.231
Rui Zhou,Dong-Jie Cui,Qi Zhao,Kai-Kai Liu,Wen-Bo Zhao,Qian Liu,Ruo-Nan Ma,Zhen Jiao,Lin Dong,Chong-Xin Shan
The microbial spoilage of soybeans during soaking process severely deteriorates the quality of soybean products and threatens human health. Herein, water-soluble aminated zinc oxide nanoparticles (ZnO NPs) were developed to effectively control the microbial spoilage in soybeans during soaking. ZnO NPs achieved significant inactivation of three dominant spoilage bacteria ( bacillus cereus , bacillus megaterium and enterococcus faecium ) isolated from the deteriorated soybeans, which could adhere to the bacterial surface and damage the cell wall/membrane, but also generate large amounts of reactive oxygen species (ROS). Compared to two commercial ZnO, water-soluble ZnO exhibited superior antibacterial properties due to producing more ROS and bacteria-adhered ability. After ZnO NPs treatment, the content of the residual Zn (51.1 mg/kg) in soybeans was the safety standards of Zn element in soybeans products for human). Therefore, the water-soluble ZnO NPs showed great potentials as efficient and safe antimicrobial agents for soybeans preservation during soaking process.
研究领域:
食品科学
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微生物学
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纳米技术
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DOI
PubMed
高影响因子
Processes
·
2025
·Vol. 13
·期 9
·2854
·
IF 2.8
Min Wang,Chunjing Yu,Xiaoyu Zhao,Junhao Liu,Haochen Zhai,Meng Qi,Xiumei Zhang,Yinsong Liu
Microbial-enhanced oil recovery (MEOR) is a promising technology for oilfield development. To improve MEOR efficiency, two functional strains—Bacillus mucilaginosus ZZ-8 and Bacillus amyloliquefaciens ZZ-11—were isolated and purified. The growth characteristics, biosurfactant production, and crude oil emulsification performance of these strains were systematically evaluated through single-strain cultures and a co-culture system (ZZ-8: ZZ-11 = 1:1). The results demonstrated that the co-culture system exhibited superior growth and functional performance compared to monocultures. The cell-free supernatant significantly reduced oil–water interfacial tension, decreasing the contact angle from 53.56 ± 1.3° to 28.78 ± 0.82°, thereby enhancing crude oil detachment from rock surfaces and improving oil displacement efficiency. Gas chromatography (GC) analysis further confirmed the co-culture system’s pronounced degradation of long-chain alkanes (C17–C35). In oil sand washing experiments, the 1:1 mixed-strain fermentation broth achieved a crude oil elution rate of 84.39%, representing an 89.80% increase over uninoculated medium. This study not only validates the synergistic effect of the B. mucilaginosus–B. amyloliquefaciens co-culture system in enhancing oil recovery but also provides a theoretical foundation and innovative strategy for its practical application in MEOR technology.
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Lu Zhao,Youzhi Zhou,Song Qin,Panpan Qin,Jianlin Chu,Bingfang He
β-Galactosidases can catalyze the hydrolysis of lactose and the synthesis of galacto-oligosaccharides (GOS) from lactose. The catalytic activity of β-galactosidases is usually inhibited by galactose and glucose, which hampers the complete hydrolysis of lactose in food products. In this report, a β-galactosidase (denoted as BMG) from Bacillus megaterium YZ08 without the inhibition by galactose and glucose is the first to our knowledge reported. Efficient secretory expression of BMG was successfully achieved in Bacillus subtilis WB800. With the increasing of galactose and glucose, the activity of BMG dramatically enhanced. With addition of 0.6 M galactose or 1 M glucose, the initial activity to o NPG hydrolysis was increased by 2.1-fold and 4.3-fold, respectively. Moreover, the synthetic rate of galacto-oligosaccharides was enhanced by 1.1-fold and 1.9-fold with the addition of 0.7 M galactose or glucose, respectively. The GOS (278 g/L) from 600 g/L lactose by BMG were efficiently produced within 12 h, and the substrate lactose was completely used up. These results suggest that BMG shows a potential application in the food industry.
研究领域:
生物化学
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生物技术
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食品科学
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DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2019
·
IF 2.883
Ping Zhu,Yan Zeng,Peng Chen,Yan Men,Jiangang Yang,Xiaoping Yue,Jiangang Zhang,Yueming Zhu,Yuanxia Sun
A one-pot two-enzyme reaction system was developed to produce high-fructose syrup (HFS) containing healthy rare sugar d-allulose from Jerusalem artichoke (JA). Inulin in JA was converted through the cascade reaction of a novel exo-inulinase from Bacillus velezensis (BvInu) and D-allulose 3-epimerase from Ruminococcu s sp. (RDAE). BvInu and RDAE were expressed in Bacillus subtilis and successfully secreted into the supernatant, which decrease the production cost and avoid enzyme purification. The optimal temperature and stability of extracellular BvInu significantly increased, thereby promoting the catalytic activity of the two enzymes for the cascade reaction in one pot. Inulin in JA powder was almost completely converted into monosaccharides in the treatment with the optimal ratio of BvInu : RDAE (80 : 40 U/g inulin) at 50 °C for 2 h. The ratio of D-glucose, D-fructose, and D-allulose in the product was approximately 1:3:1, and the yield on JA powder was 67%. The system exhibits potential high-valued application of non-grain crops on producing HFS with D-allulose.
研究领域:
生物技术
·
食品科学
·
酶学
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产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2021
·
IF 5.097
Shengnan Yin,Changlun Tong
Detection of Bacillus anthracis spores is of great importance for biosecurity and prevention of disease outbreaks. In this paper, a ratiometric colorimetric and fluorescent dual-mode platform for rapid, sensitive, and visual detection of dipicolinic acid (DPA), a biomarker of anthrax spores, was developed based on Eu3+-modified silver nanoparticles (AgNPs). Eu3+-modified AgNPs can induce the aggregation of AgNPs, its localized surface plasmon resonance (LSPR) absorption at 392 nm is decreased, and a new absorption peak at 524 nm emerged. Interestingly, upon the addition of DPA, it can effectively inhibit the aggregation of AgNPs–Eu3+, and the solution color changes from pink to yellow, while the red characteristic fluorescence of Eu3+ is turned on owing to the energy transfer from DPA to Eu3+ by the antenna effect. A new insight that AgNPs can also enhance the fluorescence of Eu3+ was found. Eu3+-modified AgNPs as a dual-mode platform for ratiometric colorimetric and fluorescence detection of DPA were thus fabricated. The good linearity is in the range of 1.0–25 μM with a LOD of 0.31 μM for the ratiometric colorimetric assay and 0.25–45 μM with a LOD of 17 nM for the fluorescent assay, which is greatly lower than the dose of DPA (60 μM) released by the contagious number of spores to cause anthrax. The ratiometric colorimetric and fluorescent probes were successfully applied for the detection of DPA in lake waters and Bacillus subtilis, and as low as 1.8 × 104 spores were detected.
研究领域:
生物安全
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分析化学
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环境科学
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产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Yaodi Zhu,Weijia Liu,Shijie Liu,Miaoyun Li,Lijun Zhao,Lina Xu,Na Wang,Gaiming Zhao,Qiuying Yu
In this study, we developed a novel and sensitive AgNPs self-assembled solid-phase (AgNPs-SASP) SERS substrate platform using seed-mediated and liquid interface self-assembly methods and applied the platform for the detection of three bacterial spores. Multivariate statistical analysis (HCA and LDA) were employed for identification and categorization of the obtained data. The results illustrated that AgNPs-SASP exhibited high reproducibility and Raman enhancement effect (1.43 × 10 4 ). The Raman shift bands of the three bacterial spores ranged from 400 to 1800 cm −1 demonstrating the difference in reigon and intensity. And the intensities of their Ca 2+ -DPA Raman bands are significantly different. HCA results revealed that the spectra for the three bacterial spores were statistically different while LDA completely differentiated the spectra for the three bacterial spores with 100 % sensitivity and specificity. Overall, the novel SERS platform based on AgNPs-SASP provides an effective tool for food safety risk control and detection.
研究领域:
食品科学
·
分析化学
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生物技术
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产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2025
·
IF 3.3
He Ying,Huang Ao,Liu Yun
Phospholipase D (PLD) is a valuable enzyme in industrial processes for converting phosphatidylcholine (PC) to phosphatidylserine (PS). In this study, a strain of Bacillus cereus was isolated from soil and identified through 16S rRNA sequencing. To enhance PLD activity, various mutagenesis strategies—including chemical treatment, irradiation, and their combinations—were employed, resulting in four high-activity positive mutants (C-7, I-12, CI 7–12, and IC 13–14). Among these, the CI 7–12 mutant exhibited a significantly higher enzymatic activity, showing a 3.12-fold increase (312.2%) compared to the wild-type strain. Fermentation conditions were optimied using response surface methodology (RSM), achieving a PLD activity of 35 U/mL. The enzyme demonstrated stability over a wide temperature range (30–60 °C) and pH range (6–10), with a half-life of 128 days. Kinetic analysis revealed a V max of 20.04 μmol/h and a K m of 7.13 μmol/mL, indicating efficient activity. In bioconversion experiments, the PLD-enriched fermentation broth catalyzed the conversion of PC to PS, achieving a 53.0% conversion rate and a 92.3% selectivity for PS in a two-phase system. These findings expand the potential sources of PLD and underscore its applicability for PS production in biotechnological applications.
研究领域:
微生物学
·
生物化学
·
工业生物技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）