CHEMOSPHERE
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2024
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IF 8.1
Qiuhui Qian,Qian Pu,Xin Li,XingCheng Liu,Anyu Ni,Xiaowen Han,Zejun Wang,Xuedong Wang,Jin Yan,Huili Wang
Triclosan (TCS), a prevalent contaminant in aquatic ecosystems, has been identified as a potential threat to both aquatic biota and human health. Despite its widespread presence, research into the immunotoxic effects of TCS on aquatic organisms is limited, and the underlying mechanisms driving these effects remain largely unexplored. Herein, we investigated the developmental and immune toxicities of environmentally relevant concentrations of TCS in zebrafish, characterized by morphological anomalies, histopathological impairments, and fluctuations in cytological differentiation and biomarkers following both acute (from 6 to 72/120 hpf) and chronic exposure periods (from 30 to 100 dpf). Specifically, acute exposure to TCS resulted in a significant increase in innate immune cells, contrasted by a marked decrease in T cells. Furthermore, we observed that TCS exposure elicited oxidative stress and a reduction in global m 6 A levels, alongside abnormal expressions within the m 6 A modification enzyme system in zebrafish larvae. Molecular docking studies suggested that mettl3 might be a target molecule for TCS interaction. Intriguingly, the knock-down of mettl3 mirrored the effects of TCS exposure, adversely impacting the growth and development of zebrafish, as well as the differentiation of innate immune cells. These results provide insights into the molecular basis of TCS-induced immunotoxicity through m 6 A-RNA epigenetic modification and aid in assessing its ecological risks, informing strategies for disease prevention linked to environmental contaminants.
研究领域:
水生毒理学
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分子生物学
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环境科学
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免疫学
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DOI
PubMed
高影响因子
Future Foods
·
2025
·
IF 8.2
Jiao Wu,Xiaoyan Du,Fangchao Tong,Shuangling Zhang,Jiaojie Guo,Changchun Zhu,Rui Cui,Qirong Bai,Mengyao Chen,Li Meng,Linlin Song,Haichuan Yu
Lonicerae Japonicae Flos (LJF), a widely consumed functional tea ingredient, was investigated for its phytochemical composition and bioactive properties. Ten LJF tea varieties were systematically analyzed for six bioactive compounds (chlorogenic acid, secoxyloganin, sweroside, cynaroside, isochlorogenic acid A, and isochlorogenic acid C) using high-performance liquid chromatography (HPLC) quantification. Multivariate statistical approaches including principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA), were employed, with 80% of variance being explained by PCA (PC1+PC2), while isochlorogenic acid C (VIP=1.32) and secoxyloganin (VIP=1.18) were identified by PLS-DA as the most significant markers for varietal differentiation. Comprehensive bioactivity evaluation included: (1) in vitro antioxidant capacity assessment via DPPH and ABTS∙ + assays, revealing significant free radical scavenging capacities (0.38-0.71 mmol VCE/g and 1.22-6.35 mmol TE/g, respectively); (2) in vivo anti-inflammatory evaluation using zebrafish models, demonstrating superior efficacy against pathogen-induced versus wound-induced inflammation. Meaningful relationships between specific phytochemical components and observed bioactivities were established by Spearman correlation analysis and heatmap clustering. Notably, the cultivar Fenghua No.1 exhibited optimal bioactive performance, achieving peak antioxidant values and significant anti-inflammatory effects. The results validate LJF's functional tea status while highlighting enhanced bioactivity in newer cultivars, particularly Fenghua No.1, supporting their potential nutraceutical applications.
研究领域:
植物学
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药理学
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食品科学
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DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2025
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IF 8
Xincong Su,Li Kai,Xiaowen Han,Rongzhi Wang,Xiao Yang,Xuedong Wang,Jin Yan,Qiuhui Qian,Zejun Wang,Huili Wang
Bisphenol S (BPS) and Bisphenol F (BPF), as alternatives to bisphenol A (BPA), are recognized for their endocrine-disrupting properties, but their combined immune toxicity mechanisms remain poorly understood. This study systematically evaluates the individual and joint immune toxicity effects of BPS and BPF through ADMET predictions, transgenic zebrafish models, and molecular docking analyses. The results indicate that equal effect concentration BPS and BPF act through distinct immune pathways: BPS primarily targets macrophages to mediate immune responses, while BPF significantly stimulates neutrophil proliferation and induces a stronger inflammatory response through chemokine signaling. Molecular docking studies show that BPF binds more stably to pro-apoptotic protein Mapk8 and oxidative stress-related protein Hsp90aa1, leading to significantly higher levels of apoptosis and reactive oxygen species (ROS) compared to BPS. The similarity of modes of action (MOA)between BPS and BPF based on relevant immune indicators calculated and experimentally is about 0.3; this quantitative result also proves that modes of action differ widely. Nonetheless, most of the indicators showed superimposed effects in the combined experiments, and it is noteworthy that the oxidative stress indicators (SOD, MDA) showed synergistic effects, suggesting that BPS and BPF, which have very different modes of action, are able to be risk assessed using an additive model with respect to immunity, but may exhibit synergistic risks with respect to oxidative stress. This research demonstrates that BPS and BPF induce immune toxicity via different molecular targets and pathways and highlights the need to account for their synergistic effects in risk assessments. These findings provide important insights into the immune toxicity mechanisms of BPA substitutes and the potential risks of combined exposures.
研究领域:
环境科学
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毒理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Neuroscience Bulletin
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2025
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IF 5.8
An Zihui,Yan Shuo,Wang Jiayi,Qiu Hanqing,Xie Binghua,Feng Shumei,Qiu Mengsheng,Tan Zhou
Myelin is an essential structure that facilitates rapid saltatory conduction in the nervous system. Discrepancies in myelin microstructure are a hallmark of numerous neurological disorders, rendering the assessment of myelin integrity and content an indispensable tool in clinical diagnostics and neuroscience research. Extensive research has been dedicated to scrutinizing its biochemical makeup and morphology under normal, pathological, and experimental conditions over the years. In this review, we present an updated summary of the myelin sheath's structure, composition, and developmental trajectory. We systematically enumerate and contrast eight prevalent myelin staining techniques across dimensions of sensitivity, specificity, and resolution, delving into their underlying staining principles. With an initial application of myelin histology on the mouse demyelination model, our review accentuates the accurate delineation of myelination and the microstructural analysis of the myelin sheath. Such insights are anticipated to significantly contribute to the evaluation and understanding of white matter pathologies.
研究领域:
神经科学
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组织学
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神经病学
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病理学
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生物医学研究
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产品关联
DOI
PubMed
高影响因子
WATER AIR AND SOIL POLLUTION
·
2024
·Vol. 235
·期 6
·1-12
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IF 3.8
Li Haiyang,Li Xiaran,Quan Fengyu,Chen Zhaojun,Xia Yanzhi,Xiong Zhong
Multifunctional adsorbents are significant for the treatment of water-soluble dyes in contaminated aquatic systems and oily dyes in colored organic effluents. However, the existing adsorbent for both water-soluble and oil-soluble dyes is rare and the cleanup effect is not ideal. In this study, alginic acid carbon aerogel (AACA) as an effective wide-spectrum adsorbent was prepared through wet-spinning, ion-exchanging, drying, and high-temperature carbonization. AACA obtained a specific surface area of 1046.0 m 2 g −1 and a mean pore size of 2.82 nm. The adsorption capacities of AACA for methylene blue (cationic dye), methyl orange (anion dye), and oil red O (oil-soluble dye) were 493.5, 389.5, and 244.2 mg g −1 , respectively, which were improved in comparison with those of previously reported calcium alginate and ferric alginate carbon aerogels. The adsorption kinetics of AACA for the three dyes were fit for the pseudo-second-order kinetic model and the adsorption isotherms could be explained by the Langmuir model. ACCA revealed good adsorption performance for other eleven different sorts of dyes and binary dye systems. The adsorption mechanism was mainly confirmed to be π–π interactions.
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
FISH & SHELLFISH IMMUNOLOGY
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2025
·
IF 3.9
Hai-Chuan Yu,Meng-Yao Chen,Shuang-Ling Zhang,Fang-Chao Tong,Fan-Ping Wang,Lin Wang,Jia-Long Zhang,Jiao-Jie Guo,Bing-Yang Zhang,Jia-Qi Tian,Jiao Wu
Maintaining the hematopoietic balance between erythroid and myeloid lineages is crucial for immune function and oxygen transport. However, the mechanisms underlying the disruption of this balance during acute inflammation remain unclear. In this study, we investigated the effects of Lipopolysaccharide (LPS)-induced acute inflammation on erythroid-myeloid hematopoiesis in zebrafish (Danio rerio) and elucidated the key signaling pathways involved. LPS treatment significantly suppressed erythropoiesis, as evidenced by reduced expression of gata1a and hbae3 and decreased red blood cell production in the caudal hematopoietic tissue (CHT). Conversely, myelopoiesis was enhanced, with increased neutrophil and macrophage accumulation at inflammatory sites. Bioinformatics analysis revealed that LPS modulates hematopoietic differentiation via the Jak2/Stat3-Hif1a signaling axis. Experimental validation demonstrated that LPS activates Jak2/Stat3 signaling, leading to increased expression of hif1a in zebrafish and HIF1α in human hematopoietic cell lines (K562 and THP-1). Inhibition of Jak2 with Ruxolitinib or knockdown of hif1a reversed the LPS-induced suppression of erythropoiesis and expansion of myelopoiesis. Strikingly, acute hypoxia similarly disrupted the hematopoietic balance via the Jak2/Stat3-Hif1a pathway, suggesting a conserved, stress-responsive mechanism. Our findings highlight the critical role of Jak2/Stat3-Hif1a signaling in acute inflammation-induced hematopoietic lineage bias and propose potential therapeutic strategies for hematological disorders associated with acute infection or hypoxia.
研究领域:
发育生物学
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免疫学
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血液学
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信号转导
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分子生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Ziqian Wang,Chunyu Du,Ruyu Yan,Shuying Li,Guanyu Zheng,Dahu Ding
Polyhydroxyalkanoates (PHA) are promising candidates for replacing petroleum-derived plastics; however, their high production costs limit their commercialisation. In this study, we successfully isolated an efficient PHA-producing strain from a salt lake, which was subsequently identified as Halomonas sp. WZQ-1. Notably, Halomonas sp. WZQ-1 could serve as a promising cell-factory platform for polyhydroxybutyrate (PHB) production, achieving a comparatively high PHB productivity (7.64 ± 0.4 g L −1 ) under moderate salt stress (60 g L −1 NaCl). We further realised semi-continuous PHB production in a bench-scale fermenter at a steady state by irregularly replenishing the organic substrate. The maximum PHB concentration reached 12.13 g L −1 . Finally, we realised the non-sterile conversion of typical biowastes (e.g. pomelo and cantaloupe residues) to PHB using Halomonas sp. WZQ-1. Encouragingly, 4.36 g L −1 PHB was directly obtained from the hydrolysate of pomelo residues with a characteristic melting temperature of 174.0 °C. Life cycle assessment was employed to systematically evaluate the environmental sustainability and potential challenges of biowaste-driven PHB biorefineries. Overall, our findings could serve as a pivotal step toward the commercialisation of PHB and provide a valuable reference for PHB biorefineries.
研究领域:
微生物学
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环境科学
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生物技术
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DOI
PubMed
高影响因子
eLife
·
2025
·
IF 0
Cong Chen,Tong-Yao Gao,Hua-Wei Yi,Yi Zhang,Tong Wang,Zhi-Ling Lou,Tao-Feng Wei,Yun-Bi Lu,Tingting Li,Chun Tang,Wei-Ping Zhang
Ubiquitin (Ub), a central regulator of protein turnover, can be phosphorylated by PINK1 (PTEN-induced putative kinase 1) to generate S65-phosphorylated ubiquitin (pUb). Elevated pUb levels have been observed in aged human brains and in Parkinson’s disease, but the mechanistic link between pUb elevation and neurodegeneration remains unclear. Here, we demonstrate that pUb elevation is a common feature under neurodegenerative conditions, including Alzheimer’s disease, aging, and ischemic injury. We show that impaired proteasomal activity leads to the accumulation of sPINK1, the cytosolic form of PINK1 that is normally proteasome-degraded rapidly. This accumulation increases ubiquitin phosphorylation, which then inhibits ubiquitin-dependent proteasomal activity by interfering with both ubiquitin chain elongation and proteasome-substrate interactions. Specific expression of sPINK1 in mouse hippocampal neurons induced progressive pUb accumulation, accompanied by protein aggregation, proteostasis disruption, neuronal injury, neuroinflammation, and cognitive decline. Conversely, Pink1 knockout mitigated protein aggregation in both mouse brains and HEK293 cells. Furthermore, the detrimental effects of sPINK1 could be counteracted by co-expressing Ub/S65A phospho-null mutant but exacerbated by over-expressing Ub/S65E phospho-mimic mutant. Together, these findings reveal that pUb elevation, triggered by reduced proteasomal activity, inhibits proteasomal activity and forms a feedforward loop that drives progressive neurodegeneration.
研究领域:
神经科学
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生物化学
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分子生物学
·
神经退行性疾病
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细胞生物学
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产品关联
DOI
PubMed
Journal of Water Process Engineering
·
2026
·
IF 6.6
Jing Zhang,Jiale Li,Kun Zhang,Yingyi Li,Yanting Zhang,Nan Jiang,Peng Chen,Ming Zhang,Meitong Li
Efficient separation of immiscible organic liquids and oil/water mixtures remains a critical bottleneck in industrial wastewater management due to their similar polarities and surface energies. A TiO 2 -functionalised stainless steel mesh (TiO 2 -SSM) was fabricated through a scalable sol−gel dip-coating route, with TiO 2 (8.0)-SSM (8.0 vol% TBOT) as the optimal architecture. Pre-wetting with a high-polarity liquid generates a stable liquid-infused interface that can be tuned on demand, endowing the membrane with exceptional underliquid superlyophobicity toward low-polarity liquids. The membrane separates at least 23 different liquid mixture pairs, with efficiencies exceeding 99% for 20 of these pairs and a excellent flux of 34,200 L·m −2 ·h −1 for formamide/n-hexane. The membrane exhibits exceptional durability, with <2% efficiency decline after 50 cycles, intact performance after 30 abrasion tests, and robust resistance to acid, alkali, and saline environments. Notably, TiO 2 (8.0)-SSM combines robust mechanical/chemical stability with remarkable photocatalytic activity, achieving >98.3% degradation of MB solution under simulated solar irradiation. This polarity-switchable TiO 2 (8.0)-SSM enables simultaneous separation and degradation of complex wastewater containing both immiscible liquids and soluble pollutants, offering a cost-effective solution for industrial wastewater treatment and resource recovery.
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DOI
高影响因子
AQUATIC TOXICOLOGY
·
2023
·
IF 4.5
Xin Li,Weiwei Wang,Xuedong Wang,Huili Wang
Herein, we demonstrated that sublethal-dose exposure to triclosan (TCS) and triclocarban (TCC) triggered larval zebrafish immunotoxicity. Acute exposure to TCS induced significant increases in larval neutrophils and macrophages and a prominent decrease in thymic T cells. In contrast, three kinds of cells (neutrophils, macrophages, and thymic T cells) were significantly reduced under TCC exposure, suggesting that both TCS and TCC suppress thymus development and mature T-cell differentiation. TCC was confirmed to have more severe immunotoxicity than TCS. Using Illumina RNA-Seq, 581 and 738 differentially expressed genes (DEGs) were identified in the TCS and TCC treatments, respectively. GO function and KEGG pathway enrichment analyses revealed that the DEGs were not identical in terms of biological processes, cellular components and molecular functions, but were primarily involved in immune response. KEGG analysis showed that approximately 47% and 11% of DEGs were mainly enriched in the immune system of the TCC and TCS treatments, respectively. Protein-protein interaction (PPI) network analysis confirmed that the hub genes enriched in the immune-related pathways differed between TCS and TCC exposure. The hub genes were fynb, mapk12b, scarb1, pik3r2, prkg3, srfa, arhgef2, cldn15la , and cldn15lb in the TCS treatment, and plg, serping1, masp2, fgg, vtnb, mmp9, serpine1, il1b, sb:cb37 and stat3 in the TCC treatment. Molecular docking simulation demonstrated that both TCS and TCC were stably docked with their target hub genes, and that their target molecules for inducing immunotoxicity were different. The differential target molecules and action pathways induced by TCS and TCC exposure provide us with diagnostic targets and toxicological endpoints.
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Xiaoyun Sun,Ke Shi,Siqi Mo,Jinfeng Mei,Jian Rong,Shaomang Wang,Xudong Zheng,Zhongyu Li
A reinforced-concrete-structured sponge based on amino-terminated polydimethylsiloxane (PDMS) and carboxyl carbon nanotubes (CCNTs) is synthesized, without any templates or coatings. The covalent bonding of PDMS and CCNTs generate amide groups which provide considerable hydrogen bonds. The synergistic effect of dynamic hydrogen bonds and flexible PDMS chains contribute to the high elasticity, while CCNTs affect the stress. The compressive and tensile stress-train curves both overlap with the first curves during multiple load-release cycles, at the compressive strain of 80% and the tensile strain of 100%, respectively. The amount of CCNTs determines the porosity, and significantly affects the hydrophobicity. The sponge is applied to static oil adsorption, oil/water emulsion separation and oil-spill treatment. Due to the high elasticity and anti-fatigue performance, the sponge can be repeatedly regenerated using a simple squeezing method. The adsorption capacity still reaches 99.45% after 30 adsorption–desorption cycles. The weight, morphology and hydrophobicity of the sponge maintain unchanged, either. The simulation of oil-spill treatment demonstrates that the sponge can also work efficiently under dynamic conditions. The high elasticity, recyclability and stability propose the sponge to be a promising material for oily-water treatment in complex circumstances.
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产品关联
DOI
高影响因子
ACS Applied Polymer Materials
·
2022
·
IF 4.855
Yan Yu,Jing Wang,Xing-wang Wang,Yan Huang,Ren-yun Li,You-xiang Wang,Ke-feng Ren,Jian Ji
Blood contact medical devices require a lubricant and anticoagulant surface to reduce tissue trauma, thrombosis, and other complications. Hydrogel coating is a promising strategy but usually shows poor adhesion and weak mechanical properties, leading to the failure of the coating by delamination or fracture during clinical applications. Herein, we report a tough and slippery hydrogel coating through the procedures of photoinitiator entrapment, pregel solution dip-coating, and UV-curing. The surface-embedded initiator and in situ polymerization attribute to the robust interfacial bonding, and double-network (DN) hydrogel formulation improves the toughness of the hydrogel coating itself. We characterize the morphology, composition, thickness, lubrication, and anticoagulant capabilities of the coating. Furthermore, we confirm the toughness of the coating by sonicating, soaking, repeated friction, tension, and the bending test. Results show that the DN hydrogel coating has a low coefficient of friction (∼0.03) after 3000 cycles of friction with a 20 N load (∼33 kPa). This coating method may bring clinical benefits in the use of vascular catheters as well as other interventional devices.
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DOI
高影响因子
危险品化学品经营许可证（带存储）