EUROPEAN JOURNAL OF PHARMACOLOGY
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2022
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IF 5.195
Yang Lin,Zi-Yuan Wang,Ma-Jie Wang,Zheng-Meng Jiang,Ya-Qiu Qin,Tian-Qing Huang,Yu Song,Hui-Ting Liang,E-Hu Liu
In this work, we examined whether baicalin (BC), a bioactive flavonoid in Scutellaria baicalensis Georgi, can reduce high-fat diet (HFD)-induced metabolic syndrome (MetS) in mice. The UPLC-QTOF/MS was used for metabolome profiles analysis, and an analysis of bacterial 16S rDNA in feces was used to examine the effects of BC on gut microbiota composition. Our results showed that BC (400 mg/kg) could reduce the body weight gain, decrease hepatic fat accumulation and abnormal blood lipids, and increase insulin sensitivity after 8 weeks of treatment. BC could reverse the alteration of 7 metabolites induced by HFD and the metabolic pathways responsive to BC intervention including citrate cycle, alanine , aspartate and glutamate metabolism, glycerophospholipid metabolism, and aminoacyl-tRNA biosynthesis. 16S rDNA analysis demonstrated that BC altered the composition and function of gut microbiota in MetS mice. Notably, we found that the change in succinic acid was negatively associated with the changes in Bacteroides and Sutterella , and positively associated with the change in Mucispirillum . Moreover, we confirmed that succinic acid displayed a metabolic protective effect on MetS mice. The antibiotic treatment verified that BC exert s metabolic protection through gut microbiota. Our findings suggested BC may be a potential therapeutic drug to ameliorate diet induced MetS and gut microbiome may be a novel mechanistic target of BC for treatment of MetS.
研究领域:
药理学
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代谢组学
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微生物学
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营养学
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产品关联
DOI
PubMed
高影响因子
Foods
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2023
·Vol. 12
·期 9
·1772
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IF 5.2
Shihua Wu,Xianxiang Chen,Ruixin Cai,Xiaodie Chen,Jian Zhang,Jianhua Xie,Mingyue Shen
This study aimed to test the preventive anti-inflammatory properties of Chinese yam polysaccharides (CYP) and sulfated Chinese yam polysaccharides (SCYP) on LPS-induced systemic acute inflammation in mice and investigate their mechanisms of action. The results showed that SCYP can efficiently reduce plasma TNF-α and IL-6 levels, exhibiting an obvious anti-inflammation ability. Moreover, SCYP reduced hepatic TNF-α, IL-6, and IL-1β secretion more effectively than CYP, and significantly altered intestinal oxidative stress levels. In addition, a 16S rRNA gene sequencing analysis showed that CYP regulated the gut microbiota by decreasingDesulfovibrioandSutterellaand increasingPrevotella. SCYP changed the gut microbiota by decreasingDesulfovibrioand increasingCoprococcus, which reversed the microbiota dysbiosis caused by LPS. Linear discriminant analysis (LDA) effect size (LEfSe) revealed that treatment with CYP and SCYP can produce more biomarkers of the gut microbiome that can promote the proliferation of polysaccharide-degrading bacteria and facilitate the intestinal de-utilization of polysaccharides. These results suggest that SCYP can differentially regulate intestinal flora, and that they exhibit anti-inflammatory effects, thus providing a new reference to rationalize the exploitation of sulfated yam polysaccharides.Keywords:yam polysaccharide;sulfation;LPS;anti-inflammation;gut microbiota
研究领域:
免疫学
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微生物学
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药理学
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DOI
PubMed
高影响因子
Journal of Functional Foods
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2025
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IF 4
Huilin Deng,Yaozhong Zheng,Qiongyao Wang,Jiaqi Peng,Weibin Bai,Lingmin Tian,Zouyan He,Rui Jiao
Mangiferin (MGF), a C-glycosyl xanthone from the mango plant, offers various health benefits but has low absorption, mostly remaining in the gut where it interacts with microbes. This study examines MGF's effects on human gut microbiota and metabolite profiles after in vitro digestion and fermentation with feces. Findings indicate MGF had limited permeability (under 2 %) across the Caco-2 monolayer, with most being fermented by microbes. MGF utilization increased post-digestion and fermentation, significantly altering gut metabolite profiles by boosting lipid and benzenoid abundance while reducing organic acids. It also promoted beneficial bacteria like Bacteroides and Bifidobacterium , decreasing harmful genera such as Sutterella . Metabolomic analysis revealed enhanced pathways for unsaturated fatty acid synthesis and longevity, primarily through oleic and linolenic acids, highlighting MGF's role in modulating gut health.
研究领域:
胃肠科学
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微生物学
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营养生物化学
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代谢组学
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产品关联
DOI
高影响因子
FEBS Open Bio
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2023
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IF 2.6
Di Wang,Wenbao Wang,Xue Bing,Chenguang Xu,Jiahua Qiu,Jiangang Shen,Jinwen Huang,Junda Li,Pi Liu,Biao Xie
Ulcerative colitis (UC) is a recurrent inflammatory disease related to gut microbiota disorder. Metabolites and their sensors play an important role in the communication between gut microbes and their host. Our previous study revealed that G protein-coupled receptor 35 (GPR35) is a key guardian of kynurenic acid (KA) and a core element of the defense responses against gut damage. However, the mechanism remains unknown. In this study, a DSS-induced rat colitis model was established and 16S rRNA sequencing was applied to explore the influence of GPR35-mediated KA sensing on gut microbiota homeostasis. Our results demonstrated that GPR35-mediated KA sensing is a necessary component in maintaining gut barrier integrity against DSS-induced damage. Furthermore, we provide compelling evidence suggesting that GPR35-mediated KA sensing plays a crucial role in maintaining gut microbiota homeostasis, which contributes to alleviation of DSS-induced colitis. In addition, five classes (Actinobacteria, Beta-/Gamma-proteobacteria, Erysipelotrichi, and Coriobacteriia) and six genera ( Corynebacterium , Allobaculum , Parabacteroides , Sutterella , Shigella , and Xenorhabdus ) were identified as the marked bacterial taxa that characterized the progression and outcome of colitis and are regulated by GPR35-mediated KA sensing. Our findings highlight that GPR35-mediated KA sensing is an essential defense mechanism against disorder of gut microbiota in UC. The results provide insights into the key role of specific metabolites and their monitor in maintaining gut homeostasis.
研究领域:
免疫学
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微生物学
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胃肠病学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
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