ELECTROCHIMICA ACTA
·
2020
·
IF 6.215
Qiaoxia Zhang,Haoyuan Lyu,Ning Li,Qing Liu,Xianxi Zhang,Xiao Zhang,Xiutong Wang,Qingyun Liu
In this work, a sensitive signal-off photoelectrochemical (PEC) thrombin aptasensor with satisfying specificity is proposed, in which the evident initial photocurrent response is produced by the photoanode and the signal amplification strategy toward the target is based on the specific bio-affinity properties between thrombin and their aptamers. The photoanode was fabricated with uniform TiO 2 nanotube arrays decorated with CuO x and perylene-3,4,9,10-tetracarboxylic acid (PTCA). After the decoration of CuO x and PTCA, both possessing a strong absorption in visible region, the photocurrent response is improved greatly. Pt nanoparticles were modified on the photoanode electrode to immobilize thrombin aptamers, which could obviously improve the conductivity of the photoanode electrode owing to the excellent conductivity of Pt nanoparticles. The non-conductive complex leads to dramatical decrease in the photocurrent value due to the steric hinder effect. Based on the joint action of evident photocurrent response of photoanode and significant signal amplification strategy, the aptasensor in this project offers a wide linear range from 0.0003 nM to 10 nM, with a detection limit (3S/N) of thrombin down to 55 fM. Moreover, the as-fabricated aptasensor exhibits acceptable selectivity and stability, providing a meaningful attempt for the detection of thrombin.
研究领域:
生物传感
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分析化学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Miao Zhang,Rana Dalapati,Jiangfan Shi,Chenglong Liao,Qingyun Tian,Chuanyi Wang,Xiaomei Yang,Shuai Chen,Marc D. Porter,Ling Zang
This paper describes the development and proof-of-concept testing of an easy-to-use trace analysis technique, namely F-SPE, by coupling fluorescent sensor with solid phase extraction (SPE). F-SPE is a two-step methodology that concentrates an analyte from a liquid sample onto a fluorophore-modified membrane and measures the amount of analyte from the extent the extracted analyte quenches the emission of the fluorophore. By applying the principle of negligible depletion (ND) intrinsic to SPE , the procedure of F-SPE for analyzing a sample can be markedly simplified while maintaining the ability to detect analytes at low limits of detection (LOD). The merits of this approach are demonstrated by impregnating a SPE membrane with a perylene diimide (PDI) fluorophore, N,N′-di(nonyldecyl)-perylene-3,4,9,10-tetracarboxylic diimide (C9/9-PDI), for the low-level detection of organic amines (e.g., aniline) and amine-containing drugs (e.g., Kanamycin). The sensing mechanism is based on the donor-acceptor quenching of PDI by amines, which, when coupled with the concentrative nature of SPE, yields LODs for aniline and Kanamycin of 67 nM (∼6 ppb) and 32 nM (∼16 ppb), respectively.
研究领域:
分析化学
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环境科学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2014
·Vol. 16
·期 48
·26917-26928
·
IF 4.198
Mingguang Li,Jiangang Liu,Xinxiu Cao,Ke Zhou,Qiaoqiao Zhao,Xinhong Yu,Rubo Xing,Yanchun Han
Herein, balanced intermixed and pure crystalline phases in N,N′-bis(1-ethylpropyl)-perylene-3,4,9,10-tetracarboxylic diimide (EP-PDI)-based non-fullerene organic solar cells (OSCs) were achieved via selective solvent additives (SAs). Poly[[4,8-bis[(2-ethylhexyl)oxy]benzo[1,2-b:4,5-b′]dithiophene-2,6-diyl][3-fluoro-2-[(2-ethylhexyl)carbonyl]thieno[3,4-b]thiophenediyl]] (PTB7) and 7,7′-(4,4-bis(2-ethylhexyl)-4H-silolo[3,2-b:4,5-b′]dithiophene-2,6-diyl)bis(6-fluoro-4-(5′-hexyl-[2,2′-bithiophen]-5-yl)benzo[c][1,2,5]thiadiazole) (F-DTS) possessing different compatibilities with EP-PDI were selected as model systems to investigate the guideline of SAs selection for different non-fullerene-based systems. According to the solubility parameter difference (Δδ) between EP-PDI and SAs, five different SAs were divided into two types: (I) strong intermolecular interactions with EP-PDI molecules (with Δδ values less than 5 MPa1/2), (II) weak intermolecular interactions with EP-PDI molecules (with large Δδ values). For PTB7:EP-PDI system with large and obvious phase separation, the introduction of type (II) SAs provided extra interactions with EP-PDI molecules, thus effectively reducing EP-PDI aggregate domains and increasing intermixed fractions. The incorporation of type (II) SAs resulted in a greater yield of dissociated polarons, and the final device efficiency increased from 0.02% to 1.65%. On the contrary, for finely mixed F-DTS:EP-PDI systems, type (I) SAs were considerably more effective because of the fact that the required pure crystalline phases were readily induced by the unfavorable interactions. The charge transport pathways optimized by type (I) SAs improved device efficiency from 0.18% to 2.82%. Hence, by processing selective SAs, the fraction of intermixed and pure crystalline phases for PDI-based non-fullerene OSCs can be well regulated; therefore, the final performance for both systems can be significantly improved.
研究领域:
材料科学
·
有机电子学
·
太阳能
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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