Foods
·
2022
·Vol. 12
·期 1
·94
·
IF 5.561
Jingling Guo,Pan Wang,Yifan Cui,Xiaosong Hu,Fang Chen,Chen Ma
Resveratrol (RSV), a polyphenolic stilbene, has been widely studied for its protective effects against non-alcoholic fatty liver disease (NAFLD) by modulating intestinal microbiota. The microbial metabolites after RSV supplement would contribute to the bioeffects of RSV, while their impacts on NAFLD were unclear. Therefore, this study aimed to investigate the beneficial effects of the main microbial metabolites from RSV on lipid metabolism by combining in vitro and in vivo models. The mice were fed a high-fat diet and injected with RSV, 3-hydroxyphenyl propionic acid (3-HPP), and 4-HPP for 13 weeks (n= 6). Body weight, serum parameters, histological analysis, and gene expression involved in lipid metabolism were quantified. Our results suggested that 100 μM of 3-HPP and 4-HPP inhibited lipid accumulation more significantly than parent RSV in an oleic acid-induced HepG2 cell line. Furthermore, 3-HPP, 4-HPP, and RSV effectively reduced liver weight and body weight, improved hepatic steatosis, and alleviated systemic inflammation in NAFLD mice. In addition, the results of quantitative real-time PCR showed that 3-HPP and 4-HPP altered the expression of cholesterol influx and efflux genes to a stronger extent than RSV. These results indicate that 3-HPP and 4-HPP are effective in regulating hepatic lipid metabolism.Keywords:NAFLD;microbial metabolites;3-hydroxyphenyl propionic acid;4-hydroxyphenyl propionic acid;lipid metabolism
研究领域:
药理学
·
肝病学
·
微生物学
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DOI
PubMed
高影响因子
FUEL
·
2016
·
IF 3.611
Chun-feng Mao,Rong-xiang Zhao,Xiu-ping Li
A series of Brønsted-Lewis acidic deep eutectic solvents (DESs) of C 9 H 10 O 2 ·XZnCl 2 (X from 0.5 to 3) were synthesized by simple mixing. The DESs were characterized by infrared spectroscopy, electrospray ionization mass spectrometry, and thermogravimetric analysis. The desulfurization of a model oil was investigated using C 9 H 10 O 2 ·XZnCl 2 as the extraction agent and catalyst, and H 2 O 2 as the oxidant. The effects of the molar ratio of phenylpropanoic acid to zinc chloride, reaction temperature, oxygen to sulfur (O/S) molar ratio, and volume ratio of DESs to oil on the desulfurization rate were investigated. The optimal conditions for desulfurization were determined to be a phenylpropanoic acid to zinc chloride ratio of 2:1, a reaction temperature of 50 °C, an O/S ratio of 6, a DESs to oil volume ratio of 0.25:1, and a model oil dosage of 5 mL. Under the optimal conditions, a desulfurization rate of 99.23% in 80 min was achieved. The recyclability of the DESs was also investigated, and the results showed that the desulfurization rate decreased slightly after five runs. These results demonstrate that C 9 H 10 O 2 ·0.5ZnCl 2 DESs exhibit high desulfurization activity and stability in extraction and oxidation desulfurization systems.
研究领域:
化学工程
·
环境科学
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DOI
高影响因子
Horticulture Research
·
2021
·
IF 6.793
Zhou Wei,Shi Min,Deng Changping,Lu Sunjie,Huang Fenfen,Wang Yao,Kai Guoyin
Water-soluble phenolic acids are major bioactive compounds in the medicinal plant species Salvia miltiorrhiza. Phenolic acid biosynthesis is induced by methyl jasmonate (MeJA) in this important Chinese herb. Here, we investigated the mechanism underlying this induction by analyzing a transcriptome library of S. miltiorrhiza in response to MeJA. Global transcriptome analysis identified the MeJA-responsive R2R3-MYB transcription factor-encoding gene SmMYB1. Overexpressing SmMYB1 significantly promoted phenolic acid accumulation and upregulated the expression of genes encoding key enzymes in the phenolic acid biosynthesis pathway, including cytochrome P450-dependent monooxygenase (CYP98A14). Dual-luciferase (dual-LUC) assays and/or an electrophoretic mobility shift assays (EMSAs) indicated that SmMYB1 activated the expression of CYP98A14, as well as the expression of genes encoding anthocyanin biosynthesis pathway enzymes, including chalcone isomerase (CHI) and anthocyanidin synthase (ANS). In addition, SmMYB1 was shown to interact with SmMYC2 to additively promote CYP98A14 expression compared to the action of SmMYB1 alone. Taken together, these results demonstrate that SmMYB1 is an activator that improves the accumulation of phenolic acids and anthocyanins in S. miltiorrhiza. These findings lay the foundation for in-depth studies of the molecular mechanism underlying MeJA-mediated phenolic acid biosynthesis and for the metabolic engineering of bioactive ingredients in S. miltiorrhiza.
研究领域:
植物学
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分子生物学
·
药用植物研究
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DOI
PubMed
高影响因子
FITOTERAPIA
·
2019
·
IF 2.431
Jingwen Li,Menglong Duan,Xinsheng Yao,Danmei Tian,Jinshan Tang
Phytochemical studies of the air-dried pericarp of Citrus grandis led to the isolation of four new compounds including three prenylated benzenepropanoic acids ( 2 , 3 and 5 ) and one alkamidic glycoside ( 6 ), together with ten known compounds ( 1 , 4 and 7 – 14 ). The structures of these compounds were determined by the NMR spectroscopy, optical rotation data and modified Mosher's method. Meanwhile, the anti-neuroinflammatory activities of all isolated compounds were evaluated by detecting the production of nitric oxide (NO) in LPS-stimulated BV2 cells. The results showed that compounds 1 , 2 , 5 and 13 exhibited strong inhibition effects on NO production in LPS-stimulated BV2 cells. Mechanistically, compounds 1 , 2 and 5 could suppress the expressions of iNOS. In addition, compounds 1 , 2 and 5 also showed obvious inhibition effects on COX-2 expression, another vital enzyme in the inflammation process, in LPS-treated BV2 cells. These findings shed light on the potent anti-neuroinflammatory effects of Citrus grandis .
研究领域:
药理学
·
神经科学
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植物化学
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产品关联
DOI
PubMed
高影响因子
Polymer Chemistry
·
2018
·Vol. 9
·期 42
·5215-5223
·
IF 4.927
Wei Yuan,Weiwen Chi,Ting Han,Jun Du,Hongkun Li,Yongfang Li,Ben Zhong Tang
Cu(I)-mediated and metal-free click polymerizations of azides and terminal alkynes have been developed into powerful techniques for constructing linear and hyperbranched polytriazoles. However, the 1,3-dipolar cycloaddition of azides with internal alkynes is rarely employed as a synthetic tool to prepare functional polymers. In this work, metal-free phenylpropiolate-azide polycycloaddition was developed for efficient synthesis of poly(phenyltriazolylcarboxylate)s (PPTCs), a kind of polyester. The aromatic and aliphatic diphenylpropiolates could readily react with diazides under heating, affording soluble PPTCs with high molecular weights (Mw up to 28 500) in excellent yields (up to 98%). The polymerization is insensitive towards oxygen and moisture, and can proceed smoothly in an open atmosphere. The obtained PPTCs are thermally stable with 5% weight loss temperatures higher than 318 °C. As the polymers contained ester groups, they exhibited fast degradation under basic conditions. The PPTC containing tetraphenylethene moieties shows a unique phenomenon of aggregation-induced emission, and can be employed as a fluorescent chemosensor to sensitively detect explosives. Its thin film can generate two-dimensional fluorescent photopatterns by UV irradiation.
研究领域:
材料科学
·
高分子化学
·
有机化学
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产品关联
DOI
高影响因子
Polymers
·
2020
·Vol. 12
·期 9
·1977
·
IF 3.426
Kexin Nie,Shanshan Han,Jianmin Yang,Qingqing Sun,Xiaofeng Wang,Xiaomeng Li,Qian Li
Soft tissue engineering has been seeking ways to mimic the natural extracellular microenvironment that allows cells to migrate and proliferate to regenerate new tissue. Therefore, the reconstruction of soft tissue requires a scaffold possessing the extracellular matrix (ECM)-mimicking fibrous structure and elastic property, which affect the cell functions and tissue regeneration. Herein, an effective method for fabricating nanofibrous hydrogel for soft tissue engineering is demonstrated using gelatin–hydroxyphenylpropionic acid (Gel–HPA) by electrospinning and enzymatic crosslinking. Gel–HPA fibrous hydrogel was prepared by crosslinking the electrospun fibers in ethanol-water solution with an optimized concentration of horseradish peroxidase (HRP) and H2O2. The prepared fibrous hydrogel held the soft and elastic mechanical property of hydrogels and the three-dimensional (3D) fibrous structure of electrospun fibers. It was proven that the hydrogel scaffolds were biocompatible, improving the cellular adhesion, spreading, and proliferation. Moreover, the fibrous hydrogel showed rapid biodegradability and promoted angiogenesis in vivo. Overall, this study represents a novel biomimetic approach to generate Gel–HPA fibrous hydrogel scaffolds which have excellent potential in soft tissue regeneration applications.Keywords:fibrous hydrogel;enzymatic crosslinking;soft tissue engineering
研究领域:
组织工程
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生物材料科学
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生物医学工程
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DOI
PubMed
高影响因子
Food & Function
·
2018
·Vol. 9
·期 9
·4858-4864
·
IF 3.289
Bo Chen,Jie Zhou,Qilu Meng,Yang Zhang,Shihua Zhang,Liang Zhang
Tea polyphenols (TP) have many health benefits, but most are metabolized into low molecular-weight phenolic acids after oral administration. In the present study, the absorption, metabolism, and excretion of catechins in rats fed a normal chow diet and in obese rats fed a high-fat and high-sugar (HFHS) diet were compared. After a ten-day oral administration of TP (500 mg per kg bw), the plasma levels of (−)-epigallocatechin gallate (EGCG) and (−)-gallocatechin gallate (GCG) in obese rats were significantly lower than those in the normal group. In obese rats, the fecal levels of EGCG, (−)-epicatechin gallate (ECG) and GCG were significantly enhanced. Ten phenolic metabolites of TP were quantitatively analyzed, and the results showed that 4-hydroxyphenylacetic acid was the primary metabolite in feces and plasma. The plasma and fecal concentrations of 4-hydroxyphenylacetic acid in the obese group were significantly lower than those in normal rats, but the levels of 4-hydroxyphenylpropionic acid in plasma and feces were increased. The content of other phenolic acids was also dramatically changed. These results suggested that a HFHS diet might influence the excretion of tea catechins, leading to insufficient metabolism of catechins by the gut microflora.
研究领域:
营养学
·
药理学
·
代谢组学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2015
·
IF 2.912
Yang Li,Guisheng Zhou,Shihua Xing,Pengfei Tu,Xiaobo Li
Echinacoside (ECH) is one of the representative phenylethanoid glycosides. It is widely present in plants and exhibits various bioactivities. However, the extremely low oral bioavailability of ECH in rats implies that ECH may go through multiple hydrolysis steps in the gastrointestinal tract prior to its absorption into the blood. Therefore, the gastrointestinal metabolites of ECH are more likely to be the bioactive components. This study established an approach combining ultraperformance liquid chromatography–quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) with MSE technology and MetaboLynx software for rapid analysis of the ECH metabolic profile produced by human intestinal bacteria. As a result, 13 ECH metabolites and 5 possible metabolic pathways (including deglycosylation, dehydroxylation, reduction, hydroxylation, and acetylation) were identified. Furthermore, hydroxytyrosol (HT) and 3-hydroxyphenylpropionic acid (3-HPP) were found to be the two bioactive metabolites of ECH produced by human intestinal bacteria.
研究领域:
药理学
·
微生物学
·
分析化学
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DOI
PubMed
高影响因子
Acta Pharmaceutica Sinica B
·
2021
·
IF 11.614
Rui Li,Li Xie,Lei Li,Xiaojiao Chen,Tong Yao,Yuanxin Tian,Qingping Li,Kai Wang,Chenyang Huang,Cui Li,Yifan Li,Hongwei Zhou,Neil Kaplowitz,Yong Jiang,Peng Chen
Hepatic ischemia/reperfusion injury (HIRI) is a serious complication that occurs following shock and/or liver surgery. Gut microbiota and their metabolites are key upstream modulators of development of liver injury. Herein, we investigated the potential contribution of gut microbes to HIRI. Ischemia/reperfusion surgery was performed to establish a murine model of HIRI. 16S rRNA gene sequencing and metabolomics were used for microbial analysis. Transcriptomics and proteomics analysis were employed to study the host cell responses. Our results establish HIRI was significantly increased when surgery occurred in the evening (ZT12, 20:00) when compared with the morning (ZT0, 08:00); however, antibiotic pretreatment reduced this diurnal variation. The abundance of a microbial metabolite 3,4-dihydroxyphenylpropionic acid was significantly higher in ZT0 when compared with ZT12 in the gut and this compound significantly protected mice against HIRI. Furthermore, 3,4-dihydroxyphenylpropionic acid suppressed the macrophage pro-inflammatory response in vivo and in vitro . This metabolite inhibits histone deacetylase activity by reducing its phosphorylation. Histone deacetylase inhibition suppressed macrophage pro-inflammatory activation and diminished the diurnal variation of HIRI. Our findings revealed a novel protective microbial metabolite against HIRI in mice. The potential underlying mechanism was at least in part, via 3,4-dihydroxyphenylpropionic acid-dependent immune regulation and histone deacetylase (HDAC) inhibition in macrophages.
研究领域:
免疫学
·
胃肠病学
·
药理学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHYSICAL CHEMISTRY B
·
2021
·
IF 2.991
Lichuang Zhang,Hao Xu,Ziyi Cheng,Yan Wei,Ruize Sun,Ziwei Liang,Yinchun Hu,Liqin Zhao,Xiaojie Lian,Xia Li,Di Huang
Surface modification of nanoparticles with cellular protein components is a new biomimetic modification strategy, which utilizes the inherent affinity between homologous cells to introduce the same surface molecules into nanoparticles to improve the targeting performance. In this study, oleic acid (OA)-coated Fe3O4 nanocubes were prepared by a high-temperature thermal decomposition method and modified by 3, 4-dihydroxyphenylpropionic acid (DHCA); then, HeLa cell membranes were introduced onto the surface of the nanocubes through mixed coextrusion to try to endow them with the targeting function of natural cells. The results show that the prepared Fe3O4 nanocubes have high monodispersity, excellent water solubility, and biocompatibility. Moreover, the Fe3O4 nanocubes encapsulated by cellular protein show an obvious core–shell structure and the specific targeting property to HeLa cells is improved significantly, which is expected to be used in clinical targeted diagnosis and treatment of cancer.
研究领域:
纳米技术
·
癌症研究
·
生物医学工程
·
材料科学
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DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2025
·
IF 4.7
Yang Yang,Jingjing Zhang,Fei Kong,Yunyang Zhang,Jian Ge,Jingwu Song,Changyu Shao,Haoran Li,Yunjiu Ren,Chengyao Ni,Xianghui Kong,Yiming Ni,Xiangdong Kong,Ruibo Zhao
In the absence of effective therapeutics, valve replacement remains the sole intervention for severe calcific aortic valve disease (CAVD), which impacts 26% of adults aged 65 and even 50% of adults aged 85. Pericardium-based bioprosthetic heart valves benefit from marginating anticoagulation. Still, they face recalcification due to residual aldehydes and inadequate elastin stabilization from the glutaraldehyde cross-linking process, posing a significant clinical challenge. Herein, we propose a biprotein stabilized pericardium material (CHPP) that involves both collagen and elastin cross-linked by procyanidins (PC) with the assistance of 3,4-hydroxyphenylpropionic acid (HPA), which conferred improved stability to pericardium even under enzymatic attack, increasing biocompatibility and minimizing platelet absorption, and taken together significantly contributed to resisting calcification. Further, in vivo studies demonstrated the preservation of the structure of CHPP, and more importantly, CHPP can prevent surrounding tissue infiltration and immune responses, reducing the infiltration of macrophages and T cells, along with their cascading inflammatory processes and establishing an immune-privileged microenvironment that further enhances its anticalcification capacity. These advancements offer an enhancing approach using procyanidins to significantly stabilize biprotein components within the pericardium, thereby creating immune tolerance against calcification and providing an alternative for robust generation of valve materials.
研究领域:
生物医学工程
·
心血管医学
·
生物材料
·
组织工程
·
免疫调节
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Lili Hao,Shijia Zhao,Shiqi Hao,Yuxin He,Miao Feng,Ke Zhou,Yiyan He,Jiquan Yang,Hongli Mao,Zhongwei Gu
Three-dimensional (3D) bioprinting is a promising technique to construct heterogeneous architectures that mimic cell microenvironment. However, the current bioinks for 3D bioprinting usually show some limitations, such as low printing accuracy, unsatisfactory mechanical properties and compromised cytocompatibility. Herein, a novel bioink comprising hydroxyphenyl propionic acid-conjugated gelatin and tyramine-modified alginate is developed for printing 3D constructs. The bioink takes advantage of an ionic/covalent intertwined network that combines covalent bonds formed by photo-mediated redox reaction and ionic bonds formed by chelate effect. Benefiting from the thermosensitivity of gelatin and the double-crosslinking mechanism, the developed bioink shows controllable rheological behaviors, enhanced mechanical behavior, improved printing accuracy and structure stability. Moreover, the printed cell-laden hydrogels exhibit a homogeneous cell distribution and considerable cell survival because the pre-crosslinking of the bioink prevents cellular sedimentation and the visible light crosslinking mechanism preserves cell viability. Further in vivo studies demonstrate that resulting cell-laden hydrogels are beneficial for the reduction of inflammation response and the promotion of collagen deposition and angiogenesis, thereby improving the quality of skin wound healing. This convenient and effective strategy is of great significance for accelerating the development of multifunctional bioinks and broadening the biomedical applications of 3D bioprinting.
研究领域:
生物医学工程
·
组织工程
·
生物材料
·
伤口愈合
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）