Frontiers in Microbiology
·
2023
·
IF 5.2
Junyuan Qin,Lei Yu,Fu Peng,Xin Ye,Gangmin Li,Chen Sun,Fang Cheng,Cheng Peng,Xiaofang Xie
Staphylococcus aureus is an opportunistic pathogen. Due to the widespread use and abuse of antibiotics, various drug-resistant strains of S. aureus have emerged, with methicillin-resistant Staphylococcus aureus (MRSA) being the most prevalent. Bacterial biofilm is a significant contributor to bacterial infection and drug resistance. Consequently, bacterial biofilm formation has emerged as a therapeutic strategy. In this study, the chemical constituents, antimicrobial and antibiofilm properties of tannins isolated from Penthorum chinense Pursh (TPCP) were investigated. In vitro , TPCP exhibited antimicrobial properties. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) for methicillin-sensitive Staphylococcus aureus (MSSA) and MRSA were 156.25 and 312.5 μg/mL, and 312.5 and 625 μg/mL, respectively. According to the growth curves, TPCP significantly inhibited the growth of MSSA and MRSA. The results of the crystal violet biofilm assay in conjunction with confocal laser scanning and scanning electron microscopy demonstrated that TPCP destroyed preformed MSSA and MRSA biofilms. TPCP significantly decreased the secretion of exopolysaccharides and extracellular DNA. Subsequently, the mechanism was investigated using RT-PCR. Examining the expression of ica A, cid A, sig B, agr A, and sar A genes in MRSA, we discovered that TPCP inhibited biofilm formation by affecting the quorum-sensing system in bacteria. Our study demonstrates that TPCP exerts antibacterial effects by disrupting the formation of bacterial biofilms, suggesting that TPCP has clinical potential as a novel antibacterial agent for the prevention and treatment of MSSA and MRSA infections.
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JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Ying Wang,Xiaoguo Ma,Yuqi Peng,Yiyang Liu,Hengyuan Zhang
Selective and fast adsorption of five broad-spectrum β-lactam antibiotics included amoxicillin, cephalexin, cefazolin, penicillin G and oxacillin was achieved by novel surface molecular imprinting polymers (MIPs) with magnetic hybrid carbon material as the substrate. The characteristics of MIPs were studied by scanning electron microscope , Fourier transform infrared spectrometer , thermogravimetric analysis , etc. And through a series of adsorption experiments to examine the kinetics, isotherms, thermodynamics, selectivity and reusability for sorption of β-lactam antibiotics onto the MIPs. The adsorption equilibriums were accomplished in about 60 min with adsorption capacities of 4.57–24.55 mg g −1 , while the adsorption process was preferably fitted with pseudo-second-order kinetic model and Freundlich model. The imprinting factors ranged from 1.88 to 9.94 indicated the MIPs possessed excellent recognition ability, and its good reusability was demonstrated after five times adsorption–desorption cycles without a large drop in adsorption capacity. Furthermore, a new detection method was developed by combining of MIPs with solid phase extraction and high performance liquid chromatography (MIPs-SPE-HPLC). Under the optimal SPE conditions, the limits of detection (LODs) for the five BLAs were 0.24–0.56 µg L −1 , with RSDs of 0.76–5.39%. The synthesized MIPs and the proposed MIPs-SPE-HPLC method can be applied for the efficient, simultaneous separation and detection of BLAs.
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Nanoscale
·
2015
·Vol. 7
·期 39
·16381-16388
·
IF 7.394
Yanni Chen,Yongwei Wang,Liqiang Liu,Xiaoling Wu,Liguang Xu,Hua Kuang,Aike Li,Chuanlai Xu
A novel gold immunochromatographic assay (GICA) based on anti-β-lactam receptors was innovatively developed that successfully allowed rapid and simultaneous detection of fifteen β-lactams in milk samples in 5–10 minutes. By replacing the antibodies used in traditional GICA with anti-β-lactam receptors, the difficulty in producing broad specific antibodies against β-lactams was overcome. Conjugates of ampicillin with BSA and goat anti-mouse immunoglobulin (IgG) were immobilized onto the test and control lines on the nitrocellulose membrane, respectively. Since goat anti-mouse IgG does not combine with receptors, negative serum from mice labelled with gold nanoparticles (GNP) was mixed with GNP-labelled receptors. Results were obtained within 20 min using a paper-based sensor. The utility of the assay was confirmed by the analysis of milk samples. The limits of detection (LOD) for amoxicillin, ampicillin, penicillin G, penicillin V, cloxacillin, dicloxacillin, nafcillin, oxacillin, cefaclor, ceftezole, cefotaxime, ceftiofur, cefoperazone, cefathiamidine, and cefepime were 0.25, 0.5, 0.5, 0.5, 1, 5, 5, 10, 25, 10, 100, 10, 5, 5, and 2 ng mL−1, respectively, which satisfies the maximum residue limits (MRL) set by the European Union (EU). In conclusion, our newly developed GICA-based anti-β-lactam receptor assay provides a rapid and effective method for one-site detection of multiple β-lactams in milk samples.
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