ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Qi Liu,Shushu Ding,Rui Gao,Guoyue Shi,Anwei Zhu
ATP-sensitive potassium (K ATP ) channels couple intracellular metabolism to the electrical activity by regulating K + flux across the plasma membrane, thus playing an important role in both normal and pathophysiology. To understand the mechanism of ATP regulating biological ion channels, developing an ATP-responsive artificial nanochannel is an appealing but challenging topic because K ATP channel is a heteromultimer of two subunits (potassium channel subunit (Kir6.x) and sulfonylurea receptor (SUR)) and exhibit dynamic functions with adjustability and reversibility. Inspired by the structure of K ATP channels, we designed a smart copolymer modified nanochannel that may address the challenge. In the tricomponent poly( N -isopropylacrylamide) (PNIPAAm, PNI)-based copolymer system, phenylthiourea was used to bind the phosphate units of nucleotides and phenylboronic acid was introduced to combine the pentose ring of the nucleoside unit. Besides, a –COOH group with electron-withdrawing property was added into the phenylthiourea units, which may promote the hydrogen-bond-donating ability of thiourea . Specially, the smart copolymer not only provided static binding sites for recognition but also translated the recognition of ATP into their dynamic conformational transitions by changing the hydrogen-bonding environments surrounding PNIPAAm chains, thus achieving the gating function of nanochannel, which resembled the integration and coordination of Kir6.x and SUR units in active K ATP . The ATP-regulated ion channel exhibited excellent stability and reversibility. This study is the first example showing how to learn from nature to assemble the ATP-responsive artificial nanochannel and demonstrate the possible mechanism of ATP gating.
研究领域:
生物化学
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材料科学
·
分子生物学
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DOI
高影响因子
POLYHEDRON
·
2018
·
IF 2.067
Xianghua Zeng,Chunhua Gong,Haiyang Guo,Hao Xu,Junyong Zhang,Jingli Xie
A novel phenylthiourea modified Mn-Anderson polyoxometalate , [N(C 4 H 9 ) 4 ] 3 [MnMo 6 O 18 {(OCH 2 ) 3 CNHCSNHPh}{(OCH 2 ) 3 CNH 2 }] ( 1 ) has been synthesized and characterized by IR, NMR, UV–Vis spectroscopy, thermogravimetric, electrochemical and single-crystal X-ray diffraction analysis. Structural analysis reveals that compound 1 crystallizes in the orthorhombic crystal system , space group Pcab with a = 17.7477(9) Å, b = 33.6245(16) Å, c = 32.322(2) Å, V = 19288.7(19) Å 3 and Z = 8. Noticeably, the asymmetric POM cluster with terminal modification could be connected through H-bonding interactions of C H⋯O to form an infinite supramolecular chain. Furthermore, the adjacent 1D chains are linked by H-bonding interactions of N H⋯O to generate a 2D supramolecular layer.
研究领域:
无机化学
·
材料科学
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DOI
高影响因子
Molecular Catalysis
·
2019
·
IF 2.938
Chaokun Yang,Yanglin Chen,Ping Xu,Li Yang,Jiaxu Zhang,Jianmin Sun
Multifunctional monocomponent zinc halide-based ionic liquids, amidinothiourea-ZnI 2 (ATUI) and N-phenylthiourea-ZnI 2 (NTUI), were facilely developed as homogeneous catalysts for efficient conversion of CO 2 with epoxides to cyclic carbonates. On account of synergistic effects of multifunctional components in ATUI, the propylene carbonate yield could reach 95% under 110 °C, 1.0 MPa, 4.0 h or even mild conditions. There was a slight decrease of propylene carbonate yield because of the loss of ATUI during the reuse process. Additionally, the universality of ATUI was also studied to other terminal epoxides. Meanwhile, a plausible reaction mechanism was proposed where the synergistic effects involving cation structure and halogen anion of ATUI guarantee the cycloaddition reaction to proceed effectively under solvent and co-catalyst free condition. Finally, reaction kinetics were undertaken to compare the catalytic efficiency of ATUI and NTUI so as to compare the catalytic activity of different numbers of hydrogen bond donor in amidinothiourea and N-phenylthiourea.
研究领域:
化学
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材料科学
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环境科学
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产品关联
DOI
高影响因子
Nano-Micro Letters
·
2024
·Vol. 17
·期 1
·1-14
·
IF 31.6
Ma Mengen,Zhang Cuiling,Ma Yujiao,Li Weile,Wang Yao,Wu Shaohang,Liu Chong,Mai Yaohua
Gas quenching and vacuum quenching process are widely applied to accelerate solvent volatilization to induce nucleation of perovskites in blade-coating method. In this work, we found these two pre-crystallization processes lead to different order of crystallization dynamics within the perovskite thin film, resulting in the differences of additive distribution. We then tailor-designed an additive molecule named 1,3-bis(4-methoxyphenyl)thiourea to obtain films with fewer defects and holes at the buried interface, and prepared perovskite solar cells with a certified efficiency of 23.75%. Furthermore, this work also demonstrates an efficiency of 20.18% for the large-area perovskite solar module (PSM) with an aperture area of 60.84 cm 2 . The PSM possesses remarkable continuous operation stability for maximum power point tracking of T 90 > 1000 h in ambient air.
研究领域:
材料科学
·
可再生能源
·
太阳能电池技术
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产品关联
DOI
PubMed
高影响因子
Journal of Pharmaceutical Analysis
·
2023
·
IF 8.8
Chen Hong,Yifan Zhang,Lili Yang,Haoyang Xu,Kang Cheng,Zhi Lv,Kaixian Chen,Yiming Li,Huali Wu
Epimedin B (EB) is one of the main flavonoid ingredients present in Epimedium brevicornum Maxim., a traditional herb widely used in China. Our previous study showed that EB was a stronger inducer of melanogenesis and an activator of tyrosinase (TYR). However, the role of EB in melanogenesis and the mechanism underlying the regulation remain unclear. Herein, as an extension to our previous investigation, we provide comprehensive evidence of EB-induced pigmentation in vivo and in vitro and elucidate the melanogenesis mechanism by assessing its effects on the TYR family of proteins (TYRs) in terms of expression, activity, and stability. The results showed that EB increased TYRs expression through microphthalmia-associated transcription factor-mediated p-Akt (referred to as protein kinase B [PKB])/glycogen synthase kinase 3β (GSK3β)/β-catenin, p-p70 S6 kinase cascades, and protein 38 (p38)/MAP kinase (MAPK) and extracellular regulated protein kinases (ERK)/MAPK pathways, after which EB increased the number of melanosomes and promoted their maturation for melanogenesis in melanoma cells and human primary melanocytes/skin tissues. Furthermore, EB exerted repigmentation by stimulating TYR activity in hydroquinone- and N -phenylthiourea-induced TYR inhibitive models, including melanoma cells, zebrafish, and mice. Finally, EB ameliorated monobenzone-induced depigmentation in vitro and in vivo through the enhancement of TYRs stability by inhibiting TYR misfolding, TYR-related protein 1 formation, and retention in the endoplasmic reticulum and then by downregulating the ubiquitination and proteolysis processes. These data conclude that EB can target TYRs and alter their expression, activity, and stability, thus stimulating their pigmentation function, which might provide a novel rational strategy for hypopigmentation treatment in the pharmaceutical and cosmetic industries.
研究领域:
药理学
·
化妆品学
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细胞生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Xiuru Zhang,Zhuangzhuang Liu,Yuna Li,Jingqin He,Lulu Yang,Yulin Yan,Shanshan Tong,Yeqi Wang,Tao Zhang
Lead (Pb), a persistent heavy metal, has caused widespread environmental contamination due to its extensive industrial use. Among its compounds, lead acetate (PbAc) is particularly hazardous in aqueous form, exhibiting neurotoxic potential. However, its toxic effects on the integrity of the blood-brain barrier (BBB) and the underlying mechanisms remain poorly understood. This study demonstrates that zebrafish embryos exposed to varying concentrations of PbAc exhibit dose-dependent morphological abnormalities, including growth retardation, delayed hatching, and cardiovascular dysfunction, along with pronounced neurobehavioral deficits in both embryonic and adult zebrafish. Live imaging analysis revealed that both acute and chronic exposure to different concentrations of PbAc can induce cerebral hemorrhage and BBB dysfunction. Additionally, PbAc selectively disrupted cerebral angiogenesis without affecting trunk intersegmental vessels and significantly reduced pericyte coverage within both cerebral and trunk vessels. Mechanistically, RNA sequencing and qPCR validation revealed significant upregulation of MMP-9 and MMP-13 in PbAc-exposed embryos. Pharmacological inhibition of MMP-9/13 using polygalacic acid significantly reduced cerebral hemorrhage and restored pericyte coverage, suggesting that these proteases mediate PbAc-induced cerebrovascular damage. Overall, our findings underscore the ecological and public health risks associated with lead contamination and provide a basis for future therapeutic strategies targeting MMPs to mitigate heavy metal toxicity.
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Fei Fang,Bo Peng,Yanjuan Zhu,Wenping Xu,Yang Zhang,Jiagao Cheng
Comprehensive understanding the toxic effects of pesticide with relative long half-life properties are always hot research topics in the world. In this study, the immunotoxic effects of Bixafen (BIX), a prominent Succinate Dehydrogenase Inhibitor (SDHI) fungicide, were systematically assessed at concentrations of 0.05, 0.1, and 0.2 μM, using a zebrafish ( Danio rerio ) larval model. Neutral red staining and confocal microscopy quantification showed that BIX exposure reduced immune cell numbers in a concentration-dependent manner. qPCR and Western blot validation revealed upregulated pro-inflammatory factors ( IL-1β , IL-8 , TNF-α ) and TLR4 gene expression, while the lymphocyte marker gene rag1 was downregulated. Protein - level detection found significantly increased p-p65/p65 and TLR4 expression. Molecular docking experiments showed that BIX forms multiple interactions with the TLR4 protein. RNA-Seq analysis further confirmed that BIX interferes with DNA replication and metabolic pathways. Our results suggest that BIX may activate proinflammatory responses and disrupt immune cell homeostasis via the TLR4 signaling pathway, posing a potential immunotoxicity risk to non-target organisms. These findings provide insights into the molecular mechanisms of BIX-induced immunotoxicity and are critical for assessing the environmental safety and health risks associated with SDHI fungicides.
研究领域:
毒理学
·
环境科学
·
免疫学
·
分子生物学
·
水生毒理学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2026
·Vol. 27
·期 5
·2111
·
IF 5.6
Mengting Xu,Yunmiao Ma,Mengxin Liu,Yueqiu Chen,Zongyi Duan,Zhenya Shen,Yanchao Han
Congenital valve defects account for a substantial proportion of cardiovascular malformations, yet the molecular mechanisms orchestrating cardiac valve development remain incompletely elucidated. While Bone morphogenetic protein (BMP) signaling is essential for valvulogenesis, the specific contributions of individual BMP ligands, particularly the teleost-specificbmp16, have not been characterized. Using the CRISPR/Cas9 system, we generated abmp16null knockout and delineated critical roles of this ligand in valvular morphogenesis.bmp16knockout embryos display a significant reduction in Sox9-positive valvular cells and exhibit severely dysplastic arterial valves, characterized by increased interleaflet distance, thickened leaflets, and shortened leaflet lengths. These morphological abnormalities correlate with impaired valve function, culminating in progressive blood regurgitation, ventricular dilation, and pericardial edema. Mechanistically, loss ofbmp16or pharmacological inhibition of BMP signaling significantly downregulatesnotch1bexpression in developing valves, while pharmacological activation of Notch signaling rescues the regurgitation phenotype inbmp16mutants. Collectively, our findings establishbmp16as a novel regulator of valve development and uncover a functional BMP-Notch signaling axis required for vertebrate valvulogenesis, providing new insights into the molecular mechanisms that govern cardiac valve formation and pathogenesis.
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Zhao-Yun Wang,Li-Xin Yu,Yu-Qing Feng,Lei Jin,Jia-Qiang Yang,Yi Zhao,Tao Song,DongPing Zhan,Daquan Yu,Fang-Zu Yang,Shi-Gang Sun
Leveler is the key additive for achieving microvia void-free filling in the high-density integration of electronic components for miniaturization, lightweighting, and intelligent development of electronic devices. Traditional organic levelers are mostly quaternary ammonium salts and nitrogen-containing heterocyclic compounds. In this study, thiosemicarbazide (TA) and 1-phenyl 2-thiourea (PTA), which are the thioamino compounds with different substituents of amino and phenyl, are used for the first time as levelers for microvia filling in an acidic copper sulfate bath of Cu electroplating (Cu-EP). The results demonstrate that only the PTA, when used as the leveler in combination with poly(ethylene glycol) (PEG) as the suppressor and bis(sodium sulfopropyl)-disulfide (SPS) as the accelerator, can achieve microvia void-free filling. Chronopotentiometric analyses reveal that both TA and PTA accelerate the reduction of cupric ions. The accelerator of SPS with TA synergistically enhances accelerating effect, while with PTA, it behaves with competitive adsorption to weaken this effect. The suppressor of PEG can cooperate with PTA to show a strong inhibiting effect on cupric ion reduction but presents competitive adsorption with TA, weakening the inhibiting effect of PEG. Under such complex interactions of these additives, it has been found that only the combination of PTA, SPS, and PEG exhibits convection-dependent adsorption behavior, which results in a more negative reduction potential of cupric ions at the mouth of the microvia than that at the bottom of the microvia, indicating a theoretical feasibility of achieving microvia void-free filling. Cyclic voltammetry experiments further demonstrate that the PTA can accelerate the reduction of cupric ions, but this acceleration effect is weakened by SPS due to competitive adsorption. PTA synergizes with PEG to greatly enhance the inhibiting effect on cupric ions reduction. According to electrochemical properties and interactions of the additives, the essence of the PTA-based acidic Cu sulfate bath for achieving microvia void-free filling is elucidated through electroplating simulations. When the three additives coexist, the adsorption coverage of PTA–PEG gradually decreases from the mouth to the bottom. As PTA–PEG exhibits a synergistic inhibition effect, such a gradient significantly inhibits Cu deposition at the mouth; simultaneously, the adsorption coverage of SPS, which behaves with an accelerating effect, gradually increases from the mouth to the bottom, facilitating the reduction of cupric ions at the bottom. The dual gradient effects enable the microvia void-free filling.
研究领域:
材料科学
·
电化学
·
电子器件
·
表面工程
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产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2025
·
IF 5.6
Jia-Qiang Yang,Tao Song,Zhao-Yun Wang,Lei Jin,Yi Zhao,Ren Hu,Jian-Jia Su,Fang-Zu Yang,Dongping Zhan,Lianhuan Han
Metal Interconnection has been becoming the core technology in integrate circuit (IC) industry at post-Moore's law era from chip manufacturing through advanced 3D packaging to high density interconnection (HDI) of printed circuit boards (PCB), which is realized by electroplating copper (Cu) to fill the micro/nano-vias with various aspect ratio. However, the rational screening of Cu-electroplating additives remains challenges because there is no sufficient instrumental method for the real-time observation of the dynamic filling process. To this, we designed a visible electrolytic cell integrated with a microvia chip and recorded the dynamic microvia filling process in operando with a high-resolution video camera. The effects of suppressor, accelerator and leveler and the technique parameters were evaluated in a seeing-is-believing way, and were examined by the practical microvia filling experiments in PCB. This instrumental method, opens the “black box” of microvia filling process for metal interconnection, provides the most direct way for in-situ screening electroplating additives and will definitely facilitate the fundamental research and technical development of metal interconnection of IC manufacturing.
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产品关联
DOI
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
·
2025
·
IF 4.3
Yunshuo Chen,Yachan Gong,Dan Li,Weiwei Feng,Yao Chen,Ting Zhao,Liuqing Yang,Guanghua Mao,Xiangyang Wu
As a typical neonicotinoid insecticide, dinotefuran (DIN) has immunotoxicity, but its immunotoxicity effects on aquatic organisms and its molecular mechanisms are not known. In this study, zebrafish embryos were used as model to reveal its immunotoxicity and its mechanism from the perspective of inflammatory response. Zebrafish larvae were exposed to DIN at environmentally relevant concentrations (0, 2, 200, and 2000 mg/L) for 120 h, followed by comprehensive analyses of immune cell populations, immune marker activities, oxidative stress levels, and gene expression profiles. The results showed that the number of neutrophils and macrophages, two types of innate immune cells, was significantly reduced, and the activities of the immune markers, lysozyme (LYS), immunoglobulin M (IgM), and complement protein C3, were significantly inhibited. The level of oxidative stress in zebrafish larvae was significantly elevated and antioxidant enzyme activities were markedly inhibited in a dose-dependent manner after exposure to DIN. In this case, pro-inflammatory cytokines such as TNF-α, IL-6 and IL-1β were also abnormally expressed, and the expression levels of the genes related to the NF-κB pathway and the JNK-STAT pathway, tlr4a, myd88, nf-κb p65, jak1, jak2 , and stat3 , were elevated. The expression levels of genes related to the antioxidant signaling pathway Nrf2-keap1 signaling pathway were suppressed. Taken together, our results suggest that DIN exposure causes damage to the immune system of zebrafish embryos-larvae, generating oxidative stress and inflammatory responses that lead to immunotoxicity.
研究领域:
环境科学
·
毒理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TOXICOLOGY LETTERS
·
2025
·
IF 2.9
Xiaoyu Mao,Dashuang Mo,Yuqin Cheng,Mengzhu Lv
N -(1,3-Dimethylbutyl)- N ′-phenyl- p -phenylenediamine (6PPD), a commonly used antioxidant in tire manufacturing, has been widely detected in the environment and shown to exhibit acute toxicity in several organs. However, the effects of 6PPD on immune responses, particularly following injury, remain poorly understood. In this study, we investigated the impact of 6PPD exposure on immune responses using zebrafish as a model. 6PPD exposure disrupted caudal fin regeneration at various stages of the regenerative process. Further analysis revealed that 6PPD impaired immune responses following fin amputation, as evidenced by the reduced number of lyz + /mpx + neutrophils and the downregulation of key immune-related genes. Besides, the morphology of neutrophils was changed upon 6PPD exposure, indicating the defective migration of immune cells. The incubation of zebrafish larvae with lipopolysaccharide (LPS), which induces global immune responses, also exhibited impaired immune function when combined with 6PPD exposure. Additionally, the injection of LPS into the egg yolk or trunk exacerbated immune responses at the injury site, yet 6PPD exposure significantly reduced neutrophil accumulation and downregulated the expression of immune-related genes, confirming the toxicity of 6PPD in immune responses. These findings provide new insights into the toxic effects of 6PPD on immune responses during injury, highlighting its potential to impair immune function in animals and human.
研究领域:
毒理学
·
免疫学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）