INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 3
·2053
·
IF 5.6
Tianhua He,Nikita Lykov,Xu Luo,Huiling Wang,Zhanxiang Du,Ziyi Chen,Shitian Chen,Lin Zhu,Ye Zhao,Chimeng Tzeng
Fatty liver is one of the most pervasive liver diseases worldwide. Probiotics play an important role in the progression of liver disease, but their effects on host regulation are poorly understood. This study investigated the protective effects of lactobacillus gasseri (L. gasseri) against high-cholesterol diet (HCD)-induced fatty liver injury using a zebrafish larvae model. Liver pathology, lipid accumulation, oxidative stress and hepatic inflammation were evaluated to demonstrate the changes in a spectrum of hepatic injury. Moreover, multiple indexes on host gene expression profiles were comprehensively characterized by RNA screening. The results showed that treatment withL. gasseriameliorated HCD-induced morphological and histological alterations, lipid regulations, oxidative stress and macrophage aggregation in the liver of zebrafish larvae. Furthermore, the enrichment of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway revealed that the core pathways ofL. gasseriregulation were interleukin-17 (IL-17) signaling, phosphoinositide 3-kinase (PI3K)-AKT signaling pathway, the regulation of lipolysis and adipocytes and fatty acid elongation and estrogen signaling. The genes at key junction nodes, hsp90aa1.1, kyat3, hsd17b7, irs2a, myl9b, ptgs2b, cdk21 and papss2a were significantly regulated byL. gasseriadministration. To conclude, the current research extends our understanding of the protective effects ofL. gasseriagainst fatty liver and provides potential therapeutic options for fatty liver treatment.Keywords:fatty liver;Lactobacillus gasseri;high cholesterol diet;zebrafish;IL-17
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 2
·1593
·
IF 5.6
Ji Ma,Yang Deng,Tingting Yang,Maoru Li,Jing Shang
Non-alcoholic fatty liver disease (NAFLD), defined in recent years as metabolic-associated fatty liver disease (MAFLD), is one of the most common liver diseases in the world, with no drugs on market. Esculetin (ESC) is an active compound discovered in a variety of natural products that modulates a wide range of metabolic diseases and is a potential drug for the treatment of NAFLD. In this study, we used an HCD-induced NAFLD larval zebrafish model in vivo and an FFA-induced BRL-3A hepatocyte model in vitro to evaluate the anti-NAFLD effect of ESC. Lipid lowering, anti-oxidation and anti-inflammation effects were revealed on ESC and related gene changes were observed. This study provides a reference for further study and development of ESC as a potential anti-NAFLD/MAFLD drug.Keywords:esculetin;non-alcoholic fatty liver disease;lipid-lowering;antioxidant
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JOURNAL OF MEDICINAL CHEMISTRY
·
2023
·
IF 7.3
Mingming Qi,Hui Zhong,Zhaoyan Cheng,Shujie Chen,Han Xiao,Jing Shang,Li Chen,Jianbo Sun
Activating Nrf2 through inhibiting Keap1 has been proven to alleviate oxidative stress and related diseases, including nonalcoholic fatty liver disease (NAFLD). Traditional Keap1 inhibitors could not avoid the “off-target” effects, but using proteolysis targeting chimera (PROTAC) technology to induce Keap1 degradation might be an effective strategy to find potential NAFLD improving agents. Thus, several PROTACs were designed and synthesized by harnessing CDDO as the Keap1 ligand in this study. PROTAC I-d exhibited optimal Keap1 degradation activity, which could increase the Nrf2 level and alleviate oxidative stress in free fatty acid-induced AML12 cells and the liver of mice fed with a methionine-choline-deficient diet. Moreover, compared with CDDO, PROTAC I-d displayed significant advantages in inhibiting hepatic steatosis, steatohepatitis, and fibrosis in the in vivo and in vitro models of NAFLD. In addition, PROTAC I-d also showed lower in vivo toxicity than CDDO. All these results suggested that PROTAC I-d might be a potential improving agent for NAFLD.
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CHEMOSPHERE
·
2022
·
IF 8.943
Hui Su,Xudan Dou,Dandan Xu,Li Feng,Yongze Liu,Ziwen Du,Liqiu Zhang
The massive use of personal medicines makes them widely enter the aquatic environments and cause pollution, drawing a great deal of attention over the last few years. In this study, a novel nano Fe 0 -loaded superfine powdered activated carbon (Fe 0 @SPAC) was prepared via a simple ball milling method. Fe 0 @SPAC showed a rapid and effective removal for aqueous carbamazepine (CBZ) via the process of synergistic adsorption and persulfate (PDS) activation. The removal efficiency of CBZ (30 mg L −1 ) could be up to 96% by Fe 0 @SPAC (0.05 g L −1 ) with the presence of PDS (2 mM), and the maximum pseudo-first-order rate constant was 0.12 min −1 . The performance of Fe 0 @SPAC was superior to other reported iron-bearing activator materials, and its dosage was much lower. Fe 0 @SPAC was also effective to remove other typical drug pollutants and had excellent reusability in five cycles. The loaded Fe 0 could activate PDS to generate OH and SO 4 − , which played the major role for CBZ removal. It is interesting that carbon base of Fe 0 @SPAC could also activate PDS via surface defects , making the minor contribution to CBZ degradation. Besides, Fe 0 @SPAC showed rapid and high adsorption for CBZ due to the superfine particle diameter, partially contributing to CBZ removal. Finally, the possible break sites of CBZ and its degradation pathway were proposed based on DFT theoretical calculation and product identification. Fe 0 @SPAC would be a promising material for the removal of drug pollutants, and this study may help understand the mechanisms of synergistic adsorption and persulfate activation by carbon composite material.
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JOURNAL OF MEMBRANE SCIENCE
·
2023
·
IF 9.5
Huimin Zhou,Zhiwei Qiu,Jin Zeng,Ruobin Dai,Zhiwei Wang
Polyamide nanofiltration (NF) membranes are instrumental in water reuse due to their capability to efficiently remove trace organic contaminants (TrOCs), which pose potential health risks. However, while the current NF membranes offer acceptable TrOC rejection, their water permeance, which is highly related to the process energy consumption in water reuse, is relatively low. To address this limitation, we introduced a novel NF membrane by incorporating a lignocellulosic nanofibril interlayer—specifically, nanowires with alternating hydrophilic-hydrophobic regions (denoted as HONW). The resultant NF membrane achieved remarkable water permeance (52.4 L m −2 h −1 bar −1 ) while still ensuring high TrOC retention (including endocrine-disrupting compounds, pharmaceuticals, and antibiotics). A detailed mechanistic investigation revealed that the eliminated funnel effect or introduced gutter effect induced by the HONW interlayer, improved polyamide layer structure characterized by lower cross-linking degree and higher surface wettability , and reduced interlayer resistance (due to a unique water transport pattern: hydrophilic regions absorbed water molecules while hydrophobic regions strengthened water penetration) were significantly responsible for the enhanced membrane permeance. Moreover, the membrane also demonstrated low specific energy consumption as well as outstanding stability. This investigation unveils an appealing approach for constructing the ultra-permeable NF membrane for efficient water reuse.
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WATER RESEARCH
·
2023
·
IF 12.8
Xuecong Qian,Shilong Wang,Haijun Cheng,Ziye Jia,Da Wang,Yandong Xie,Jinhao Duan,Yu Tian,Jun Ma
The oxidation kinetics of Mn(II) by free chlorine is relatively low under near-neutral pH conditions which limits the Mn removal efficiency in drinking water treatment. Therefore, this study investigated the oxidation efficiency of Mn(II) by the UV-enhanced chlorination (UV/chlorine) system and identified the responsible reactive radical species. The results show that the oxidation kinetic of Mn(II) was greatly enhanced by the UV/chlorine system under near-neutral pH or even acidic conditions. The pseudo-first-order reaction rate of Mn(II) at pH 8.0 (within the first 20 min) increased from 2.60 × 10 −5 s −1 to 3.41 × 10 −4 s −1 . Based on the scavenging experiments and the steady-state kinetic modeling, ClO · and ClO 2 , whose steady-state concentration (∼10 −10 M and ∼10 −9 M, respectively at pH 8.0) was at least 4 orders of magnitude higher than that of HO · and Cl · , were recognized as the dominant reactive species contributing to the oxidation of Mn(II). Kinetic model calculations indicate that the contribution of ClO · to the oxidation of Mn(II) was consistently maintained above 70 %, and ClO 2 also played an important role in the oxidation of Mn(II) especially under acidic and alkaline conditions. In addition, the background components of HCO 3 − and Cl − had negligible influence on the oxidation efficiency because they barely changed the concentration of the ClO · and ClO 2 . This study first demonstrates the important role of ClO 2 in the oxidation of Mn(II) in the UV/chlorine system, and the possible role of ClO 2 in the degradation of some organic pollutants needs to be carefully evaluated in the future.
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Life-Basel
·
2021
·Vol. 11
·期 5
·445
·
IF 3.817
Yang Deng,Ji Ma,Xin Weng,Yuqin Wang,Maoru Li,Tingting Yang,Zhiyang Dou,Zhiqi Yin,Jing Shang
NAFLD (non-alcoholic fatty liver disease) is one of the most prominent liver diseases in the world. As a metabolic-related disease, the development of NAFLD is closely associated with various degrees of lipid accumulation, oxidation, inflammation, and fibrosis. Ilex chinensis Sims is a form of traditional Chinese medicine which is used to treat bronchitis, burns, pneumonia, ulceration, and chilblains. Kaempferol-3-O-glucuronide (K3O) is a natural chemical present in Ilex chinensis Sims. This study was designed to investigate the antioxidative, fat metabolism-regulating, and anti-inflammatory potential of K3O. A high-cholesterol diet (HCD) was used to establish steatosis in larval zebrafish, whereby 1mM free fatty acid (FFA) was used to induce lipid accumulation in HepG2 cells, while H2O2was used to induce oxidative stress in HepG2. The results of this experiment showed that K3O reduced lipid accumulation and the level of reactive oxygen species (ROS) both in vivo (K3O, 40 μM) and in vitro (K3O, 20 μM). Additionally, K3O (40 μM) reduced neutrophil aggregation in vivo. K3O (20 μM) also decreased the level of malondialdehyde (MDA) and significantly increased the level of glutathione peroxidase (GSH-px) in both the HCD-induced larval zebrafish model and H2O2-exposed HepG2 cells. In the mechanism study,keap1,nrf2,tnf-α, andil-6mRNA were all significantly reversed by K3O (20 μM) in zebrafish. Changes in Keap1 and Nrf2 mRNA expression were also detected in H2O2-exposed HepG2 cells after they were treated with K3O (20 μM). In conclusion, K3O exhibited a reduction in oxidative stress and lipid peroxidation, and this may be related to the Nrf2/Keap1 pathway in the NAFLD larval zebrafish model.Keywords:non-alcoholic fatty liver disease;kaempferol-3-O-glucuronide;oxidative stress;inflammation;high-cholesterol diet.
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WATER RESEARCH
·
2021
·
IF 11.236
Xiangyu Chen,Juanjuan Yao,Hongsen Dong,Mingjian Hong,Naiyun Gao,Zhi Zhang,Wenchao Jiang
A collaborative system including peroxymonosulfate (PMS) activation in a photocatalytic fuel cell (PFC) with an BiOI/TiO 2 nanotube arrays p-n type heterojunction as photoanode under visible light (PFC(BiOI/TNA)/PMS/vis system) was established. Xenon lamp was used as the light source of visible light. A 4.6 times higher pseudo-first-order bezafibrate (BZF) degradation rate constant was achieved in this system compared with the single PFC(BiOI/TNA)/vis system. The radical quenching experiments revealed that the contribution of reactive oxidative species (ROS) followed the order of 1 O 2 ≈ h + >> •OH > SO 4 •− >>O 2 •− . The EPR tests demonstrated that PMS addition enlarged the formation of 1 O 2 , •OH and SO 4 •− , but suppressed O 2 •− yield. Interestingly, 1 O 2 was further proved to dominantly originated from the priority reaction between positive photoinduced holes ( h + ) and negatively charged PMS. Besides, N 2 -purging tests and density functional theory calculation indicated that PMS probably reacted with residual photoinduced electron ( e − ) on the more negative conduction band (CB) of BiOI to form •OH and SO 4 •− , but competed with dissolved oxygen. Other e − transferred to the less negative CB of TNA through p-n junction will efficiently move to cathode through the external circuit. The greatly promoted power generation of PFC system was observed after PMS addition due to extra h + consumption and efficient e − separation and transfer. Besides, three possible pathways for BZF degradation were proposed including hydroxylation, fibrate chain substituent and amino bond fracture. This study can provide new insights into the mechanisms of PMS assisted photocatalysis and accompanying energy recovery.
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International Journal of Biological Sciences
·
2019
·
IF 4.067
Ji Ma,Hongli Yin,Maoru Li,Yang Deng,Owais Ahmad,Guohong Qin,Qiangqiang He,Jiajing Li,Kai Gao,Junyi Zhu,Bing Wang,Susu Wu,Tao Wang,Jing Shang
Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease around the world. However, there is still no drug for NAFLD in the market, the study of potential therapeutic drugs on NAFLD is extraordinarily pressing and urgent. The rodent models for NAFLD drugs' study are always with a long time cost. Therefore, we aim to establish a short-time NAFLD drug screening model. A laboratory-made high cholesterol diet was used on larval zebrafish for 3 weeks to establish the NAFLD screen model. Lipid metabolism, oxidant stress, and pathology were studied to comprehensively demonstrate the whole spectrum of NAFLD on this model. Bezafibrate and pioglitazone were used to evaluate the model. Moreover, mechanism research was performed on this model.The NAFLD larval zebrafish model was established with the comprehensive process of NAFLD. Moreover, multiple index on lipid metabolism, oxidant stress, hepatic steatosis, and hepatic inflammation can be easily tested for drug screening. Furthermore, this model can be used to perform the mechanism research by testing mRNA expression. The NAFLD larval zebrafish model is a comprehensive short-time screening method for NAFLD drugs.
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JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2019
·
IF 2.813
Nadeem Muhammad,Dandan Guo,Yun Zhang,Azeem Intisar,Qamar Subhani,Muhammad Abdul Qadir,Hairong Cui
Analysis of acidic pharmaceuticals in complex biological samples is a challenging and formidable task due to the existence of interfering constituents within the sample matrices. Therefore, in order to avoid analytical column clogging and suppression/enhancement of signals of the analyte of interest, herein a simple, cost-effective and quick online ion chromatography based clean-up setup was introduced. This system was further coupled with a cost-effective homemade photochemically induced fluorimetric (PIF) setup for direct online conversion of non-fluorescent acidic pharmaceutical drugs into their respective fluorescent species. This advantageous system was favorably applied for the determination of four non-fluorescent acidic compounds in two complex biological samples (human serum and oral fluid) with minimum labor and organic solvent consumption. At optimized conditions, the developed method has shown good sensitivity, selectivity, satisfactory recoveries (88.68–102.14%) and low limits of detection (0.35–8.10 μg/L) with minimum or zero matrix effect.
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JOURNAL OF HAZARDOUS MATERIALS
·
2019
·
IF 7.65
Yang Guo,Erzhuo Zhao,Jun Wang,Xiaoyuan Zhang,Haiou Huang,Gang Yu,Yujue Wang
The abatement of several emerging contaminants (ECs) in groundwater by conventional ozonation and three ozone-based advanced oxidation processes (AOPs) - catalytic ozonation with manganese dioxide (MnO 2 ), conventional peroxone (O 3 /H 2 O 2 ), and electro-peroxone (EP) - was compared in this study. The addition of MnO 2 , H 2 O 2 , or electro-generation of H 2 O 2 during ozonation enhanced ozone transformation to hydroxyl radicals to different extent. These changes did not considerably influence the abatement of ECs with moderate to high ozone reactivities ( k O 3 ≥500 M -1 s -1 ), whose abatements were similar with >90 % during all four processes. In comparison, the abatements of ozone-refractory ECs ( k O3 < 15 M -1 s -1 ) were lower during conventional ozonation (∼40–85 % abatement), but could be enhanced by ∼10–40 % during the three ozone-based AOPs. Besides enhancing ozone-refractory EC abatement, the three AOPs, especially the O 3 /H 2 O 2 and EP processes, reduced considerably bromate formation compared to conventional ozonation. These results demonstrate that the EP process performs similarly as catalytic ozonation and O 3 /H 2 O 2 processes in terms of EC abatement and bromate control. Considering its more convenient, flexible, and safer way of operation, the EP process may provide an attractive alternative to the two more traditional AOPs for water treatment.
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JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2016
·Vol. 126
·期 2
·959-967
·
IF 1.781
Shen Chang-shuai,Zhou Cai-rong
The thermal decomposition kinetics of bezafibrate in a nitrogen atmosphere has been investigated by analyzer DTG-60. The thermal behavior of bezafibrate at the different heating rates 4, 8, 12, 16 and 20 °C min −1 displays two-stage decomposition processes among 500–650 K. The activation energy ( E ) and pre-exponential factor ( A ) were calculated to be 128.72 kJ mol −1 and 3.56 × 10 10 min −1 by Flynn–Wall–Ozawa, Kissinger–Akahira–Sunose, Doyle, Kissinger and Šatava–Šesták methods for the second-stage decomposition process. The results show that the non-isothermal thermal decomposition mechanism of bezafibrate is classified as three-dimensional diffusion model, and the integral and differential equations are \(g(\alpha ) = [1 - (1 - \alpha )^{1/3} ]^{2}\) and \(f(\alpha ) = \frac{3}{2}\left( {1 - \alpha } \right)^{{{\raise0.7ex\hbox{$2$} \!\mathord{\left/ {\vphantom {2 3}}\right.\kern-0pt} \!\lower0.7ex\hbox{$3$}}}} \left[ {1 - \left( {1 - \alpha } \right)^{{{\raise0.7ex\hbox{$1$} \!\mathord{\left/ {\vphantom {1 3}}\right.\kern-0pt} \!\lower0.7ex\hbox{$3$}}}} } \right]^{ - 1}{.}\) The enthalpy (Δ H
≠ ), entropy (Δ S
≠ ) and Gibbs free energy (Δ G
≠ ) were calculated to be 123.74 kJ mol −1 , −91.07 J mol −1 K −1 and 178.29 kJ mol −1 , respectively.
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危险品化学品经营许可证（带存储）