Molecular Medicine Reports
·
2019
·Vol. 20
·期 6
·5084-5090
·
IF 1.851
Jinsan Yang,Cao Ma,Maoshu Zhang
Diabetes mellitus adversely affects human bones and increases the risk of developing osteoporosis. In the present study, treatment with 30 mmol/l glucose was used to establish a high glucose (HG) cell model in vitro. Plasmids were used to overexpress the P2X purinoceptor 7 (P2X7) gene. Brilliant blue G and (4‑benzoyl‑benzoyl)‑ATP were used as a P2X7 antagonist and agonist, respectively. Proliferation of osteogenic MC3T3‑E1 cells and alkaline phosphatase (ALP) activity were determined using MTT and colorimetric assays, respectively. Alizarin Red S was used to assess calcification of MC3T3‑E1 cells. Western blotting and reverse transcription‑quantitative PCR were performed to determine protein and mRNA expression levels. The results demonstrated that HG inhibited MC3T3‑E1 cell proliferation and P2X7 expression, reduced calcification, and downregulated the expression levels of ALP and osteocalcin (Ocn) in MC3T3‑E1 cells. Overexpression of P2X7 in HG conditions increased calcification and proliferation, and upregulated the levels of ALP and Ocn in MC3T3‑E1 cells. Inhibition of P2X7 downregulated the expressions of ALP and Ocn in MC3T3‑E1 cells under HG conditions. Therefore, the present results indicated that HG caused damage to osteogenic MC3T3‑E1 cells. Thus, P2X7 may be a regulatory factor that may be used to counteract the effects of HG on osteogenesis.
研究领域:
内分泌学
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细胞生物学
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分子生物学
·
糖尿病研究
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骨骼生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Xuejing Lou,Yingge Hao,Huina Zhou,Zhen Song,Youmei Wang,Minghua Lu
The creation of defects in crystalline structures can tune metal-organic frameworks (MOFs) properties, such as improving their adsorptive and catalytic performance with producing more porosity and active sites. In this work, the bimetallic UiO-66 containing Zn and Zr was prepared. And then UiO-66 with missing cluster defects (UiO-66-1/3) were obtained by acid washing to remove the Zn nodes. UiO-66-1/3 was used as sorbent of dispersive solid-phase extraction (dSPE) to extract and enrich (BUs). Combination with high-performance liquid chromatography-diode array detector (HPLC-DAD) was developed to detect trace BUs in soil samples. Adsorption equilibrium can be reached in 3 min. The method possesses high enrichment factor (202-325), low detection limit (0.005-0.04 ng·mL -1 ), and wide linear range (0.02-200 ng·mL -1 ). In addition, the recovery rate of UiO-66-1/3 as an adsorbent was still higher than 95% after reused for 16 times. This work provides a new material for the enrichment and detection of benzoylurea insecticides in the environment.
研究领域:
环境科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 28
·12585-12592
·
IF 3.591
Shuwei Lin,Qiyun Tong,Pan Jiang,Baozong Li,Yi Li,Yonggang Yang
Three dipeptides based on the Ala–Ala building block with different substituent positions of the dodecyloxy group on the benzoyl residue at the N terminus were designed and synthesized (ortho-, meta- and para-16). All of them were able to spontaneously self-assemble in the mixed solvent of methanol and water (6/4, v/v). However, three dipeptides underwent a distinct kinetically controlled self-assembling process. The spectroscopic study also revealed that, for ortho-16, the phenylene groups and the peptide sequence loosely packed, while meta- and para-16 stacked into well-ordered structures with the formation of parallel β-sheets through supramolecular self-assembly. Intramolecular hydrogen bonding and steric hindrance of the dodecyloxy side chain were proposed to lead to the different self-assembling behaviour of the three isomers.
研究领域:
生物化学
·
超分子化学
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产品关联
DOI
高影响因子
Polymer Chemistry
·
2013
·Vol. 5
·期 8
·2951-2960
·
IF 5.231
Minghui He,Guangxue Chen,Xun Huang,Ruixin Xu,Zhaohua Zeng,Jianwen Yang
N-Phthaloyltranexamic acid ammonium salt derivatives integrating with peroxides can invoke redox photopolymerization and propagating polymerization. A series of N-phthaloyltranexamic acid ammonium salt derivatives based on benzophenone, thioxanthen, and naphtha chromophores as novel photocaged bases were synthesized and characterized. In combination with a benzoyl peroxide initiator, these structures were able to initiate the amine-mediated redox photopolymerization of acrylates. In particular, the covalent binding of N-phthalimidoamino acid and type II chromophores (thioxanthone, benzophenone) improved remarkably the photoreactivity of photocaged bases (thioxanthen-DBU and benzophenone-DBU) compared to the parent compound. Furthermore, naphthal-DBU matches well the emission spectra of the mercury lamp with a dominant emission wavelength at 365 nm. More importantly, phthal-DBU & BPO as a new photocaged redox combination can invoke controlled redox polymerization within an irradiation time of 3 min. Finally, due to the persistent interaction of the produced longeval amine with peroxides, remarkable post conversion after irradiation, which is significant for preparation of photo-screened materials, was thus initially achieved in the redox photopolymerization.
研究领域:
材料化学
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高分子科学
·
有机化学
✓ 已验证
DOI
高影响因子
Journal of Environmental Sciences
·
2024
·
IF 5.9
Hongxing Yang,Wangjin Yang,Xiangli Nan,Ning Tang,Chong Han
The photochemical conversion of SO 2 to sulfates on 4–(benzoyl) benzoic acid (4–BBA) was investigated deeply in the presence of anionic and cationic surfactants. The types of surfactants determined their effect behaviors, and cationic surfactants can significantly enhance the SO 2 oxidation on 4–BBA under irradiation, as shown by larger SO 2 uptake coefficients and sulfate production. Hydrophilic moieties in cationic surfactants have a greater enhancement effect on the photochemical conversion of SO 2 to sulfates than the corresponding hydrophobic moieties. Cationic surfactants obviously increased the accumulation of H 2 O on the surface, which was proved to be the main factor influencing the SO 2 uptake and the sulfate formation on 4–BBA. SO 2 lifetime and sulfate formation rate in the mixture system of photosensitive substances with surfactants were evaluated to be 2.25 days and 0.09 μg/(m 3 ·h), respectively.
研究领域:
环境科学
·
大气化学
✓ 已验证
DOI
高影响因子
CHEMISTRY AND PHYSICS OF LIPIDS
·
2012
·
IF 2.571
Songbai Liu,Huina Ruan
A highly sensitive method for quantification of phytosterols based on HPLC has been developed by derivatization with the benzoyl chromophore. Introduction of the chromophore, benzoyl group, to phytosterols via simple and inexpensive derivatization greatly improved the UV response at 254 nm. Quantification of phytosterols was effectively performed by HPLC analysis with methyl benzoate as the internal standard after derivatization. This new method demonstrated outstanding yield of recovery (>95%) and excellent sensitivity (ng level) and was applicable for sterols from either plant or animal sources. This method is generally useful in phytosterol studies.
研究领域:
分析化学
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植物学
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食品科学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 97
·95283-95291
·
IF 3.289
Lu Hu,Heng Qian,Xiaoling Yang,Songqing Li,Sanbing Zhang,Runhua Lu,Wenfeng Zhou,Haixiang Gao
A novel and simple method for effervescence-assisted dispersive liquid–liquid microextraction based on the solidification of a floating ionic liquid using a special collection method combined with high-performance liquid chromatography is developed for the determination of four benzoylureas in water samples. In this method, the ionic liquid (IL), trihexyl(tetradecyl)phosphonium tetrafluoroborate, is used rather than organic drops, which have limitations, as the extraction solvent for the first time in dispersive liquid–liquid microextraction based on the solidification of floating organic drops (DLLME-SFO). Due to the high viscosity of the IL, it can be quickly collected in the lid of the centrifuge tube after solidification, which combines the advantages of both dispersive liquid–liquid microextraction and liquid phase microextraction based on the solidification of a floating IL. Furthermore, effervescence is used to disperse the IL, thereby avoiding the use of an organic dispersant. The influence of various experimental factors, such as the form of the effervescent material, IL quantity, base to acid ratio in the effervescent tablet, weight ratio of IL to effervescent tablet, ultrasonication time, centrifugation time, extraction temperature, sample pH, extraction time and salt addition, are optimized using a central composite design and the one-factor-at-a-time approach. Under the optimal conditions, good linearity was obtained for the four BUs from 2 to 500 μg L−1, with correlation coefficients ranging from 0.9994 to 0.9995. The recoveries were 90.2–100.2% with relative standard deviations ranging from 1.3–4.4%. The limits of detection for the analytes were between 0.77 to 1.58 μg L−1, and the enrichment factors ranged from 206 to 228. Additionally, this method was successfully applied for the determination of four BUs in real water samples.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2015
·
IF 4.074
Weiqing Kong,Qingqing Dai,Junli Ren,Nianfang Ma
A new xylan ester (xylan 3,5-dinitrobenzoate) as a creatinine adsorbent was prepared by the homogeneous acylation of xylan with 3,5-dinitrobenzoyl chloride in 1-butul-3-methylimidazolium chloride ionic liquid. The influences of reaction conditions on the degree of substitution values of xylan esters were discussed. Results indicated that xylan esters with the degree of substitution range from 1.34 to 1.77 were obtained under the given conditions. The FTIR and 13 C NMR spectroscopies provided the evidence of grafting 3,5-dinitrobenzoyl groups onto the backbone of xylan. Moreover, the adsorption properties of the xylan ester for creatinine were also investigated. Isotherm studies showed that the sorption capacities for creatinine were 2.45, 2.08 and 1.86 mg/g for 23, 30 and 37 °C, respectively. Thermodynamic studies performed indicated the sorption process mainly was controlled by the chemical adsorption. Therefore, xylan 3,5-dinitrobenzoate displayed the promising application in the treatment of chronic renal failure by the creatinine adsorption as the new oral adsorbent.
研究领域:
生物化学
·
医学材料
·
药物化学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 185
·期 1
·1-8
·
IF 4.58
Wang Juntao,Jiao Caina,Li Menghua,Wang Xiaolan,Wang Chun,Wu Qiuhua,Wang Zhi
Porphyrin-based porous organic polymers (P-POPs) are amorphous polymers linked by strong covalent bonds between the porphyrin subunits that act as building blocks. The authors describe a magnetic P-POP that possesses high surface area, a highly porous structure, and strong magnetism. The MP-POP was employed as a magnetic sorbent for the extraction of benzoylurea insecticides from cucumber and tomato samples prior to their determination by HPLC. The sorbent has a typical sorption capacity of 1.90–2.00 mg∙g −1 . The method exhibits a good linear range (0.8–160 ng·g −1 ), low limits of detection (0.08–0.2 ng·g −1 ), and high method recoveries (81.8–103.5%) for cucumber and tomato samples. The MP-POP has different adsorption capabilities for the benzoylurea insecticides, phenylurea herbicides and phenols compounds, and the adsorption mechanism is found to be based on π-stacking, hydrogen-bonding, and hydrophobic interactions.
A novel magnetic porphyrin-based porous organic polymer was fabricated and used as the adsorbent for the efficient extraction of benzoylurea insecticides.
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MINERALS ENGINEERING
·
2021
·
IF 4.765
Huifang Yang,Kaihua Huang,Xiaoyu Cao,Xiaoping Huang,Zhanfang Cao,Hong Zhong,Hu Zhou,Jianxian Zeng,Jianrong Xue,Rui Zhang
The flotation performance and hydrophobic mechanism of O-ethyl-N-benzoyl thionocarbamate (EBZTC) on chalcopyrite were first estimated by single flotation experiments, batch adsorption experiments, contact angle measurements, DFT calculation and FTIR analysis. Single flotation experiments illustrated that a flotation recovery of 96.7% was achieved to chalcopyrite when EBZTC was used compared to 90% with SIBX at pH 6.5. Adsorption experiments indicated that the adsorption process of EBZTC on chalcopyrite surface was an exothermic monolayer chemisorption process. Hydrophobic mechanism demonstrated that EBZTC chemisorbed onto the chalcopyrite surface through the chemical reactions of C O and C S groups to the copper species with the formation of C O Cu and C S Cu bonds, which resulted in the coverage of the hydrophobic groups of EBZTC onto the chalcopyrite surface to enhance its hydrophobicity.
研究领域:
矿物加工工程
·
理论化学
·
材料表面科学
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产品关联
DOI
高影响因子
KOREAN JOURNAL OF CHEMICAL ENGINEERING
·
2012
·Vol. 29
·期 10
·1388-1392
·
IF 0.991
He Minqiang,Pan Aixia,Xie Jimin,Li Huamin,Yuan Xinhua,Cheng Xiaonong,Chen Min
A novel one-pot catalytic synthesis of 1-benzoylpyrene through acylation of pyrene with benzoic anhydride catalyzed by several heteropoly acids (HPAs) was investigated. Pure 1-benzoylpyrene was obtained and its structure was identified by GC/MS, FT-IR and 1 H NMR spectra. Silica-supported phosphotungstic heteropoly acid (PW/SiO 2 ) was found to be the most active catalyst in the acylation. The yield and the selectivity of 1-benzoylpyrene were up to 62.5% and 100%, respectively. The effects of experimental parameters on the catalytic acylation reaction, and the possibility of reusability of PW/SiO 2 catalyst were studied. PW/SiO 2 catalyst is easily separable from the reaction mixture and reusable without loss of its activity.
研究领域:
有机化学
·
催化材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2020
·
IF 4.049
Huixiao Duo,Xiaofen Lu,Xiaobin Nie,Licheng Wang,Shuai Wang,Xiaojing Liang,Yong Guo
Ferric oxide/carbon (Fe 2 O 3 @C) was fabricated via direct carbonization of metal-organic framework of iron (MOF-235) under argon atmosphere. The magnetic Fe 2 O 3 nanoparticles are evenly embedded in porous carbon matrix, while original morphology of MOF-235 was well-maintained. The synthesized Fe 2 O 3 @C was used as magnetic sorbent for extracting five benzoylurea insecticides (BUs). The materials exhibited excellent extraction performance, which benefited not only from the strong π-π interaction and hydrophobic interaction (π-conjugated system), but also to the abundant adsorption sites and flexible transport channel (the interconnected 3D porous structure). A three-factor-three-level Box-Behnken design (BBD) was selected to optimize three greatly influential parameters: amount of adsorbent (A), desorption time (B) and volume of desorption solvent (C) by response surface methodology. The established method coupled to HPLC-UV detection showed wide linearity with the range of 0.2–450 μg•L −1 , relatively low limits of detection (0.05–0.10 μg•L −1 ) with the relative standard deviation (RSD) ( n = 7) lower t than 5.47%. Moreover, the proposed method was successfully applied to analyze BUs in tea samples and investigate the removal effect of different washing on BUs residues from tea leaf. These results indicated that the synthesized Fe 2 O 3 @C is a promising adsorbent material for magnetic solid phase extraction of BUs at trace concentrations from tea samples.
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）