POLYMER
·
2021
·
IF 4.43
Yongjie Dong,Siqi Chen,Piao Ning,Ka Lu,Song Ma,Yingjie Wang,Shaoyu Lü
Self-healing hydrogels based on phenylboronate ester bonds are of huge interest in polymer science. However, most of these hydrogels only heal themselves at pH above their pKa (mostly > 8.0), which reduces their potential application in physiologic environments. Here, we showed that a small amount of phosphate produced stable hydrogels, able to support their self-healing at neutral pH. The hydrogel was formed by poly (3-acryloxymethylphenylboronic acid- co -N, N-dimethyl acrylamide) and poly (D-gluconamidoethyl methacrylate- co -N, N-dimethyl acrylamide) in phosphate buffer. Self-healing was observed at pH = 7.0. Intermolecular interactions between phenylboronic acid and phosphate were suggested by rheology, DSC determination and QTAIM calculations. The study represents a new mechanism to promote phenylboronate-based hydrogel formation and self-healing at neutral environment and, more importantly, provides considerable insight into their application in physiologic conditions including sensors and tissue engineering.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Huihui Li,Wei Liu,Fengyuan Zhang,Xinyue Zhu,Liqiu Huang,Haixia Zhang
The detection of tyrosinase, a biomarker for melanoma, is of great significance. Herein, a fluorescent tyrosinase probe, with resorufin as the fluorophore and m-tolylboronic acid pinacol ester as the receptor, is proposed. The response relies on the tyrosinase-catalyzed hydroxylation of phenylboronic acid pinacol ester at an adjacent position followed by 1,6-rearrangement elimination to release resorufin. This probe well quantifies tyrosinase in the range from 1 to 100 U mL–1 with a detection limit of 0.5 U mL–1. Importantly, the probe exhibits high selectivity for tyrosinase over other biological substances including reactive oxygen species. In addition, it is successfully applied to the imaging of tyrosinase in cells. This probe provides a novel platform for selective detection of tyrosinase in biosystems.
研究领域:
生物化学
·
医学检测
·
肿瘤研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 14
·2775-2780
·
IF 4.3
Jin Shanxia,Li Yaqin,Yang Liuquan,Li Wei,Zhou Ping
The affinity interactions between boronic acids and sugars have been successfully exploited in many fields, such as the sensing of saccharides, selective enrichment of glycoconjugates, and drug delivery. However, despite multiple techniques having been adopted to investigate the reaction of boronate affinity, the pathway of boronate esters formation under aqueous conditions remains controversial. We report a MALDI-MS approach to investigate the interactions between phenylboronic acid and monosaccharides in neutral aqueous solution by using polylevodopa as an innovative substrate instead of conventional matrix. A series of unusual tri-benzeneboronic esters were then revealed. The mass spectrometry data indicate that they bear a dibenzenepyroboronate cyclic ester moiety with seven-membered ring or eight-membered ring. With the aid of theoretical computations, their most likely geometrical structures are elucidated, and these tri-benzeneboronic esters are proposed to be formed via a boroxine binding monosaccharide pathway. This work provides more insight into the mechanism of boronate affinity interaction between boronic acid and sugars and proves the developed MALDI-MS approach is promising for studying interactions between small molecules. Graphical Abstract
研究领域:
分析化学
·
理论化学
·
糖化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
OPTICS EXPRESS
·
2022
·Vol. 30
·期 15
·26975-26987
·
IF 3.833
Wei Wang,Meijing Hong,Nai-Kei Wong,Jianbin Deng,Zesen Li,Yang Ran,Jie Li,Lipeng Sun,Long Jin,Bai-Ou Guan
A micro-nanostructure-based surface-modified fiber-optic sensor has been developed herein to selectively detect hydrogen peroxide (H2O2). In our design, phenylboronic ester-modified polymers were used as a modified cladding medium that allows chemo-optic transduction. Sensing is mechanistically based on oxidation and subsequent hydrolysis of the phenylboronic ester-modified polymer, which modulates hydrophobic properties of fiber-optic devices, which was confirmed during characterization of the chemical functional group and hydrophobicity of the active sensing material. This work illustrates a useful strategy of exploiting principles of chemical modifications to design surface-wettable fiber-optic sensing devices for detecting reactive species of broad relevance to biological and environmental analyses.
研究领域:
生物传感
·
环境分析
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Ming Li,Xinglei Shi,Bo Yang,Jiajun Qin,Xiaobao Han,Wanjia Peng,Yiyan He,Hongli Mao,Deling Kong,Zhongwei Gu
Hydrogel tissue adhesives that currently available are often fabricated by mixing two or more polymeric components. Single-component hydrogels afford injectability, strong and reversible adhesion remain a formidable challenge. This research describes the creation of the first single-component hyaluronic acid hydrogel adhesive-based on phenylboronic acid-diol ester linkages. Phenylboronic acid can not only serve as a cross-linker to form hydrogel, but also act as an adhesion site for glycosyl compounds found in biological cell membranes. The rheological and compressive tests for the hydrogel show that it has excellent self-healing properties, good injectability and strong compressive strength . Adhesion tests demonstrated that the hydrogel has significantly greater adhesion strength than commercial fibrin glue. These findings suggest that the rational design of hydrogel precursors facilitates the formation of single-component networks and multiple functionalities. In vivo studies further proved the hydrogel was an ideal bio-adhesive with biocompatibility, absorbed wound exudate and hemostasis , and accelerated wound closure.
研究领域:
生物材料学
·
医学工程
·
组织修复与再生
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2019
·
IF 8.355
Danzhao Guo,Shuwen Wu,Xuechao Xu,Xiangheng Niu,Xin Li,Zhenjiang Li,Jianming Pan
In this work, a novel label-free hypochlorite amperometric sensor based on the direct oxidation of benzeneboronic acid pinacol ester (BAPE) induced by hypochlorite was developed. 4-Aminophenylboronic acid pinacol ester (ABAPE) chemically bonded to functionalized multi-walled carbon nanotubes (f-MWCNTs) was employed to recognize the hypochlorite species. In nearly neutral solutions, the ABAPE probe was able to provide a distinct oxidation signal at approximately 0.65 V vs. Ag/AgCl. When hypochlorite was added, it reacted with ABAPE rapidly to form a stable hydroxyl derivative, resulting in a significant decrease of the current at 0.65 V. Therefore, the hypochlorite target could be detected by recording the reduced oxidation signal of ABAPE. Under optimized conditions, the fabricated sensor offered linear amperometric responses for the target in the concentration ranges of 0.01–0.1 mM and 0.1–1 mM, providing a limit of detection down to 5.3 μM based on S/N = 3. The presence of common anions, including carbonate, sulfate, nitrate, and halide ions, had no obvious effects on the selective determination of hypochlorite. The amperometric sensor also exhibited excellent accuracy and precision in monitoring the hypochlorite level in tap water. These results suggest that the new sensor has great promise for use in various fields such as water pollution control, environmental monitoring, and food safety.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
Biomedical Materials
·
2023
·Vol. 18
·期 5
·055001
·
IF 4
Guifei Li,Zhen Shi,Hongjie Zong,Kunxi Zhang,Shifeng Yan,Jingbo Yin
A new generation of osteochondral integrated scaffolds is needed for articular osteochondral regeneration, which can not only facilitate the accurate construction of osteochondral scaffolds in a minimally invasive manner but also firmly combine the subchondral bone layer and cartilage layer. Herein, an osteochondral integrated hydrogel scaffold was constructed by the poly(L-glutamic acid) (PLGA) based self-healing hydrogels with phenylboronate ester (PBE) as the dynamic cross-linking. The bone layer self-healing hydrogel (hydrogel O-S) was prepared by physically blending nanohydroxyapatite into the self-healing hydrogel PLGA-PBE-S, which was fabricated by 3-aminophenylboronic acid/glycidyl methacrylate-modified PLGA (PLGA-GMA-PBA) and 3-amino-1,2-propanediol/N-(2-aminoethyl) acrylamide-modified PLGA (PLGA-ADE-AP). The cartilage layer self-healing hydrogel (hydrogel C-S) was prepared by PLGA-GMA-APBA and glucosamine- modified PLGA-ADE-AP (PLGA-ADE-AP-G). Excellent injectability and self-healing profiles of hydrogel O-S and C-S were observed, the self-healing efficiencies were 97.02% ± 1.06% and 99.06% ± 0.57%, respectively. Based on the injectability and spontaneous healing on the interfaces of hydrogel O-S and C-S, the osteochondral hydrogel (hydrogel OC) was conveniently constructed in a minimally invasive manner. In addition, in situ photocrosslinking was used to enhance the mechanical strength and stability of the osteochondral hydrogel. The osteochondral hydrogels exhibited good biodegradability and biocompatibility. The osteogenic differentiation genes BMP-2, ALPL, BGLAP and COL I of adipose-derived stem cells (ASCs) in the bone layer of the osteochondral hydrogel were significantly expressed, and the chondrogenic differentiation genes SOX9, aggrecan and COL II of ASCs in the cartilage layer of the osteochondral hydrogel were obviously upregulated after 14 d of induction. The osteochondral hydrogels could effectively promote repair of osteochondral defects after 3 months post-surgery.
研究领域:
生物材料学
·
再生医学
·
骨科
✓ 已验证
产品关联
DOI
PubMed
高影响因子
METABOLIC BRAIN DISEASE
·
2025
·Vol. 40
·期 5
·1-14
·
IF 3.5
Zhao Hexiang,Yang Ping,Zhang Mou,Zheng Wenshu,Qi Renli,Zhu Xiaofeng,Li Jinghui,Li Shipeng
Reperfusion, while essential for restoring blood supply, paradoxically exacerbates neuronal damage through cerebral ischemia-reperfusion injury (CIRI). This study aimed to develop a reactive oxygen species (ROS)-responsive drug delivery system (DDS) loaded with edaravone (EDA) to enhance targeted therapy for CIRI. The stimuli-responsive DDS was synthesized using dextran (DEX) as the biocompatible carrier and benzeneboronic acid pinacol ester (BAPE) as the ROS-sensitive moiety. The physicochemical characteristics of the DEX-BAPE/EDA (DB/EDA) micelles were systematically evaluated. In vitro studies assessed the anti-inflammatory, antioxidant, and anti-apoptotic effects of DB/EDA. Moreover, the neuroprotective efficacy of DB/EDA in vivo was analyzed via behavioral tests, infarct volume measurement, ELISA assays of inflammatory cytokines and OS markers, and Western blot analysis of Nrf2-related pathways. Pharmacokinetics and biosafety were analyzed through plasma profiling and H&E staining. DB/EDA exhibited high stability, efficient drug encapsulation, and ROS-responsive drug release. Cellular uptake studies confirmed enhanced internalization of DB/EDA micelles in BV2 cells. In the oxygen-glucose deprivation/reoxygenation (OGD/R) model, DB/EDA significantly suppressed TNF-α, IL-1β, IL-6, and MDA, restored SOD levels, and attenuated apoptosis. In the middle cerebral artery occlusion/reperfusion (MCAO/R) mice, DB/EDA administration effectively improves cognition and mitigates neuronal damage. Mechanistically, DB/EDA activated the Nrf2/HO-1 pathway, amplifying antioxidant and anti-inflammatory responses. Pharmacokinetic analysis revealed prolonged circulation and increased brain accumulation, and histopathological analysis demonstrated the safety profile of DB/EDA. The ROS-responsive DB/EDA nano-micelles provided targeted EDA delivery to ischemic brain regions, alleviating CIRI via Nrf2 activation, suggesting that DB/EDA is a promising strategy for CIRI treatment. Graphical abstract
研究领域:
药剂学
·
神经科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymers
·
2023
·Vol. 15
·期 9
·1998
·
IF 5
Rong Xu,Jiafeng Tian,Yusheng Song,Shihui Dong,Yongjun Zhang
Multiple responsive hydrogels are usually constructed by the addition of many different functional groups. Generally, these groups have different responsive behaviors which lead to interleaved and complex modes of the multi-response system. It is difficult to get a practical application. In this study, we show that multi-response hydrogels can also be constructed using dynamic bonds as crosslinks. The multiple responsive hydrogel films with thicknesses on the sub-micrometer or micrometer scale can be fabricated from P(DMAA-3-AAPBA), a copolymer of N,N-dimethylacrylamide, 3-(acrylamido)phenylboronic acid, and poly(vinylalcohol) (PVA) though a simple layer-by-layer (LbL) technique. The driving force for the film build up is the in situ-formed phenylboronate ester bonds between the two polymers. The films exhibit Fabry–Perot fringes on their reflection spectra which can be used to calculate the equilibrium swelling degree (SDe) of the film so as to characterize its responsive behaviors. The results show that the films are responsive to temperature, glucose, and fructose with simple and practical linear response modes. More importantly, the speed of which the films respond to glucose or fructose is quite fast, with characteristic response times of 45 s and 7 s, respectively. These quick response films may have potential for real-time, continuous glucose or fructose monitoring. With the ability to bind with these biologically important molecules, one can expect that hydrogels may find more applications in biomedical areas in the future.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2023
·
IF 6.6
Yu Xia,Runhai Chen,Ye Ke,Qiaoyi Han,Zhifang Ma,Qiang Shi
Inflammatory and thrombotic complications, and low loading of dual drugs with different hydrophilicity remain challenges to treat thrombosis with drug delivery systems (DDS). Here, the reactive oxygen species (ROS)-responsive amphiphilic block polymer poly(ethylene glycol)-b-2-((((4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzyl)oxy)carbonyl)oxy)ethyl methacrylate (PEG-b-PTBEM) is synthesized. And the nanovesicles (PPTV) are prepared successfully for the drug delivery platform by controlling the hydrophilic/hydrophobic ratio of molecular chains and molecular self-assembly. The anti-inflammatory drug indomethacin (IDM) is loaded in the wall of nanovesicels and the thrombolytic enzyme nattokinase (NK) is encapsulated in the aqueous cavity of nanovesicles. Both drugs can be rapidly released in the site of thrombus and/or inflammation with excessive ROS concentration. The dual drug-loaded nanovesicles not only eliminate ROS, but also alleviate inflammation and dissolve generated thrombus, showing significant therapeutic efficacy in the in-vivo mouse model of carrageenan tail thrombosis. Therefore, drug-delivery nanovesicles play multiple roles in the treatment of inflammation-induced thrombotic disorders, which offers a promising treatment for inflammatory and thrombotic complications.
研究领域:
药学
·
纳米技术
·
治疗学
·
药物递送系统
·
炎症
·
血栓
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2024
·
IF 3.7
Yurong Leng,Yanmei Wu,Wenjing Xiao,Xiaoquan Su,Zhe Liu
Nanomedicine provides promising new methodologies for the treatment of tumors but still faces several limitations, including poor colloidal stability, uncontrollable drug release, and insufficient drug targeting. Herein, hyaluronic acid (HA) was used to modify the surface of mesoporous silica nanoparticles (MSNs) via a dynamic-covalent linker, phenylborate ester (PBAE), termed MA. The HA modifier provided enhanced colloidal stability to the hybrid nanoparticles. As expected, MA exhibited an improved biocompatibility and high potential for biomedical applications. Moreover, MA with a negatively charged surface effectively adsorbed the drug Doxorubicin (DOX) inside the carriers, ensuring minimal drug leakage. In an acidic and reactive oxygen species (ROS)-containing condition mimicking the tumor microenvironment, MA@DOX (MAD) continuously released its payloads, likely due to the cleavage of the pH/ROS-sensitive PBAE. Compared with free DOX, MAD had 2.2 times higher accessibility to tumor cells than free DOX. The favorable stability and cancer-selective drug release make this nanoformulation a promising platform for potent cancer treatment.
研究领域:
纳米医学
·
药学
·
癌症治疗
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
·
2023
·
IF 5.8
Lei Liu,Zhifang Ma,Qiaoyi Han,Wei Meng,Hongbo Ye,Tianci Zhang,Yu Xia,Zehong Xiang,Yue Ke,Xinghua Guan,Qiang Shi,Fazly I. Ataullakhanov,Mikhail Panteleev
The brain and liver are more susceptible to ischemia and reperfusion (IR) injury (IRI), which triggers the reactive oxygen species (ROS) burst and inflammatory cascade and results in severe neuronal damage or hepatic injury. Moreover, the damaged endothelial barrier contributes to proinflammatory activity and limits the delivery of therapeutic agents such as some macromolecules and nanomedicine despite the integrity being disrupted after IRI. Herein, we constructed a phenylboronic-decorated chitosan-based nanoplatform to deliver myricetin, a multifunctional polyphenol molecule for the treatment of cerebral and hepatic ischemia. The chitosan-based nanostructures are widely studied cationic carriers for endothelium penetration such as the blood–brain barrier (BBB) and sinusoidal endothelial barrier (SEB). The phenylboronic ester was chosen as the ROS-responsive bridging segment for conjugation and selective release of myricetin molecules, which meanwhile scavenged the overexpressed ROS in the inflammatory environment. The released myricetin molecules fulfill a variety of roles including antioxidation through multiple phenolic hydroxyl groups, inhibition of the inflammatory cascade by regulation of the macrophage polarization from M1 to M2, and endothelial injury repairment. Taken together, our present study provides valuable insight into the development of efficient antioxidant and anti-inflammatory platforms for potential application against ischemic disease.
研究领域:
纳米技术
·
药学
·
神经科学
·
肝病学
·
炎症研究
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）