Scientific Reports
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2019
·Vol. 9
·期 1
·1-11
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IF 4.011
Geng Qianqian,Li Tian,Wang Xin,Chu Weijing,Cai Mengling,Xie Jingchan,Ni Hanwen
In this work, the inclusion complexes of hydrophobic herbicide bensulfuron-methyl (BSM) with β-cyclodextrin (β-CD) and (2-hydroxypropyl)-β-CD (2-HP-β-CD) were prepared and characterized. Phase solubility study showed that both β-CD and 2-HP-β-CD increased the solubility of BSM. Three-dimensional structures of the inclusion complexes were simulated by the molecular docking method. The docking results indicated that guest BSM could enter into the cavities of host CDs, folded, and centrally aligned inside the inclusion complexes. The benzene ring of the guest molecule was close to the wide rim of the host molecules; the pyrimidine ring and side chains of the guest molecule were oriented toward the narrow rim of the host molecule. The inclusion complexes were successfully prepared by the coprecipitation method. The physiochemical characterization data of 1 H NMR, FT-IR, XRD, and DSC showed that the guest and host molecules were well included. BSM had lower soil adsorption and higher herbicidal activity in the complexation form with β-CD or 2-HP-β-CD than in the pure form. The present study provides an approach to develop a novel CDs-based formulation for hydrophobic herbicides.
研究领域:
农业科学
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环境科学
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药剂学
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DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2019
·
IF 2.87
Bojun Yan,Yuhua Zhang,Jun Li,Jiapeng Fang,Tingting Liu,Liyao Dong
Cytochrome P450s (P450s) confer resistance against herbicides, and this is increasingly becoming a concern for weed control. As a widespread Gramineae weed in paddy fields , Echinocloa glabrescens has become resistant to the acetolactate synthase (ALS)-inhibiting triazolopyrimidine herbicide penoxsulam. In this study, we found that the GR 50 of the resistant population (SHQP-R) decreased substantially from 25.6 to 5.0 and 6.2 g a.i. ha −1 after treatment with the P450 inhibitors piperonyl butoxide (PBO) and malathion , respectively. However, P450 inhibitors almost had no effects on the susceptibility of the sensitive population (JYJD-S) to penoxsulam. To investigate the mechanisms of metabolic resistance, transcriptome sequencing analysis was performed to find candidate genes that may confer resistance to penoxsulam in E. glabrescens . A total of 233 P450 differentially expressed genes (DEGs) were identified by transcriptome sequencing. We found that the metabolic process and metabolic pathways were the most highly enriched in DEGs. Further, twenty-seven candidate P450 DEGs were selected for qPCR validation analyses. After penoxsulam treatment, the relative expression levels were significantly higher in SHQP-R than in JYJD-S. Among these, the relative expression of twenty-three P450 DEGs (eighteen from the CYP72A-71C-74A-96A-734A subfamily; five from CYP81E1-94C1–94B3-714C1-714C2) were upregulated and four P450 DEGs (from CYP724B1-711A1-707A7-97B2) were downregulated. Changes in the expression of these candidate P450 genes in E. glabrescens were in response to penoxsulam, which provides preliminary evidence for the role of P450s in herbicide metabolism in E. glabrescens. However, further functional studies on metabolic resistance to penoxsulam in a resistant E. glabrescens population are required.
研究领域:
植物科学
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分子生物学
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杂草科学
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药理学
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PubMed
高影响因子
BIORESOURCE TECHNOLOGY
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2023
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IF 11.4
Yang Yang,Zhichao Kang,Jian Wang,Guanghui Xu,Yong Yu
Biochar-based bacteria are regarded as an efficient strategy for remediating organic pollutants in aquatic environments. Herein, a strain named Acinetobacter YH0317 that could degrade bensulfuron-methyl (BSM) at a lower temperature (15 °C) was isolated from a paddy rice field with long-term BSM application. Then Acinetobacter YH0317 was loaded on unmodified biochar (BC) and boron doping biochar (BBC). Results showed that BBC-based YH0317 significantly enhanced the removal efficiency of BSM (71.8–99.1%) compared with BC-based YH0317 (41.9–44.0%) and YH0317 alone (18.1–20.7%) in 24 h. BBC promoted the growth of YH0317 and secretion of extracellular secretions by providing a carrier and shelter for YH0317. The electrochemical analysis suggested BBC improved the electron transfer rate, which ultimately facilitated the removal of BSM. Hydroponic experiments indicated that BBC-based YH0317 effectively improved the growth of soybean. This work reports a novel BBC-based Acinetobacter YH0317 that could effectively remediate BSM contamination in the water environment.
研究领域:
环境科学
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微生物学
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生物修复
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农业科学
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DOI
PubMed
高影响因子
MARINE ENVIRONMENTAL RESEARCH
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2025
·
IF 3.2
Yufei Dai,Xianli Song,Jing Zhao,Limin Wang,Xiaoru Cui,Li Lu,Jingyu Zhang,Haoyu Zhang,Dahai Zhang,Keqiang Li
Pesticides occur widely in coastal waters and potentially pose a risk to marine ecosystems and human health. The spatiotemporal distribution of current-use pesticides (CUPs) was studied in Laizhou Bay (LZB) and the Xiaoqing River watershed in China during the summer of 2022 and spring of 2023. The pesticide composite index ( PCI ) and persistence index ( PI ) were established in association with the risk quotient ( RQ ) to assess the ecological risk in LZB. In this study, 17 and 12 kinds of CUP were detected in rivers and LZB seawater, respectively, with atrazine and neonicotinoid insecticides (NEOs) being the most frequently detected CUPs at higher concentrations. The average concentration of CUPs decreased from rivers to the coast and towards the center of the LZB, with higher levels in spring and lower levels in summer in rivers and a reversed pattern in seawater. The high-risk areas lie in the LZB coastal area and the estuary. Atrazine, chlorpyrifos, and imidacloprid might pose a high risk to aquatic organisms based on the RQ in the LZB, whereas the priority concern pesticides are atrazine and triazophos based on the PCI and PI . This study is of great significance for assessing the composite ecological risk of CUPs in aquatic environments.
研究领域:
环境科学
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海洋生态学
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毒理学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
·
IF 5.7
Fang Xu,Yukuai Zhang,Longfei Su,Zhenxing Guo,Qiang Cheng,Liqiang Xu,Feng Wang,Guoping Sheng
Understanding the environmental fate of chemical herbicides is crucial to sustainable agriculture. Due to their joint-use with nitrogen fertilizers, their residues often coexist with NO3– in agricultural drainages. In this study, tribenuron-methyl was used as a model to evaluate the role of NO3– in the phototransformation of chemical herbicides, which was characterized by a two-stage process. Initially, a slow hydrolysis occurs (kobs = 2.573 × 10–4 min–1), producing two hydrolysis products: methyl-2-(aminosulfonyl)-benzoate (MSB) and 2-methyl-4-methylamino-6-methoxy-1, 3, 5-triazine (MMT), which can be significantly accelerated by solar irradiation (kobs = 2.152 × 10–2 min–1). Subsequently, MSB undergoes a rapid NO3–-initiated photodegradation process (kobs = 2.251 × 10–2 min–1). MMT was identified as the refractory unit and undergoes a slow NO3–-initiated photodegradation process (kobs = 4.494 × 10–4 min–1). The underlying mechanisms were elucidated through electron paramagnetic resonance spectroscopy and reactive species quenching experiments. This study fills a knowledge gap on the interaction between NO3– and chemical herbicides, highlighting the pivotal role of NO3– in the phototransformation of chemical herbicides.
研究领域:
环境科学
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农业科学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2025
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IF 6.7
Menghan Liu,Ruyu Zhang,Jie Wang,Huiting Zhang,Jingying Tan,Qingzhi Liu,Lei Han,Lihua Lu
Bimetallic nanozymes not only have strong synergistic catalysis ability but also may improve the catalytic specificity of the nanozymes. Herein, the oxidase (OXD)-mimic Ir/Co bimetallic nanozymes were prepared through a coordination reaction between the Ir(III) complex with the 2-methylimidazole (MeIm) ligands in ZIF-67 under mild conditions without the pyrolysis and physical doping procedures. It is found that the trace amount of Ir-modified ZIF-67 (Ir@ZIF-67) possesses 18.17-fold that of catalytic ability and 7.86-fold that of the reaction rate as those of pristine ZIF-67. On the other hand, compared with ZIF-67, the OXD-like specificity of Ir@ZIF-67 has been increased 9.7%. The theoretical simulation shows that the high-performance of Ir@ZIF-67 comes from its higher O2 adsorption ability. In virtue of the high performing OXD-like catalytic property, Ir/Co bimetallic nanozymes are employed to establish a simple and sensitive colorimetric pesticides detection platform without deleterious H2O2, which offers excellent “switch-on” malathion (MAL) detection in the linear range of 0.05–25 μM with a limit of detection (LOD) of 11.8 nM. Moreover, Ir/Co bimetallic nanozymes are successfully fabricated into the MAL test swabs. Assisted by a color-reading APP, the test swabs can detect MAL with the LOD as low as 18.5 nM. Surprisingly, such a simple test swab can achieve such high sensitivity for MAL detection. The real sample analysis verifies the high potential of the test swabs in practical application. The excellent performance of the fabricated test swabs can promote the formation of the situation where pesticides detection can be tested at any time and any place and can be carried out by anyone. This work not only provides a novel approach to designing polymetallic nanozymes but also displays the promising application of Ir/Co bimetallic nanozymes in the field of colorimetric sensing.
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高影响因子
Environmental Technology & Innovation
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2025
·
IF 7.1
Xiang Li,Yumeng Dai,Xinyu Guan,Zhiyang Han,Xu Li,Xiujuan Wang,Zhencheng Su,Huiwen Zhang,Mingkai Xu
The overuse of sulfonylurea herbicides (SUHs) in agricultural environments has resulted in severe residues that damage ecosystems and harm human health. In this study, we described a microbial consortium L1, capable of broad-spectrum degradation of SUHs, which degraded 200 mg L -1 of bensulfuron-methyl, chlorimuron-ethyl, metsulfuron-methyl, tribenuron-methyl and thifensulfuron-methyl by 81.27%, 97.59%, 62.34%, 72.06% and 100%, respectively, within 7 days. The 16S rDNA and metagenome sequencing results showed that the dominant genera in consortium L1 under different SUHs were concentrated in Methyloversatilis , Pseudoxanthomonas and Chitinophaga . However, the diversity and structure of consortium L1 varied significantly under different SUHs, reflecting differences in both abundant and rare taxa, which could also interconvert. KEGG annotations showed that consortium L1 had the same functional composition when degrading different SUHs, but the number of gene annotations was significantly different, with benzoate degradation (ko00362) being the most enriched of all treatments. Furthermore, the results showed that functional redundancy existed in degradation high contribution genera, with abundant taxa having a greater impact on the degradation of different SUHs. Additionally, network analysis revealed that both rare and abundant genera had more positive edges, suggesting that the strains engaged in more mutualistic or symbiotic cooperation during the degradation process. More importantly, rare taxa play an important role in connectivity between modules. Overall, the assembly of abundant and rare taxa communities and functional composition were closely related to broad-spectrum consortium degrading. These findings deepen the understanding of bacterial consortia and provide valuable insights to enhance their potential in environmental remediation.
研究领域:
环境微生物学
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生物修复
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农药降解
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微生物生态学
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基因组学
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DOI
高影响因子
Food Bioscience
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2025
·
IF 5.9
Lu Zeng,Huan Lv,Xiangrui Li,Wenjia Yang,Wencan Luo,Linghong Kong,Qiujin Zhu
Cyclosulfamuron (CS) is a widely used sulfonylurea herbicide in plant cultivation and food production, and poses significant threats to ecological balance and food safety. To address this concern, we designed a novel CS hapten using computer-simulated semi-antigens. A monoclonal antibody (mAb) exhibiting high specificity and affinity was generated, with an affinity constant of 1.049×10 9 L/mol and a semi-inhibitory concentration (IC 50 ) of 4.94 ng/mL. Leveraging this anti-CS mAb, we constructed a rapid, visually detectable, quantitative lateral flow immunoassay (LFIA) to detect CS in cereals. Under the optimal conditions, the cut-off values were determined to be 100 μg/kg for brown rice and corn samples and 50 μg/kg for rice samples. The corresponding limits of detection values (LODs) were found to be 1.93 μg/kg in brown rice, 2.18 μg/kg in corn and 1.93 μg/kg in rice, respectively. The recovery rates ranged from 96.2% to 104.8% with coefficients of variation ranging from 3.5% to 6.4%, showing good agreement with the results obtained by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The proposed assay demonstrated robust analytical performance, with significant application potential for rapid, on-site detection.
研究领域:
食品安全
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分析化学
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免疫学
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DOI
高影响因子
Nano Research
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2024
·
IF 9.5
Lu Qianqian,Xu Xinxin,Qu Aihua,Liu Liqiang,Li Yuzhe,Sun Maozhong,Xu Chuanlai,Kuang Hua
Herbicide residues in agricultural products can have adverse effects on the environment and human health, therefore, there is an urgent need to establish a sensitive, rapid, and wide-ranging detection method. In this study, haptens of phenylurea herbicides (PUs) and sulfonylurea herbicides (SUs) were analyzed and designed based on computational simulation techniques, and two high-performance broad-spectrum monoclonal antibodies against PUs and SUs were prepared. On this basis, a multi-colloidal gold immunochromatography assay (multi-CGIA) was developed to simultaneously detect 13 herbicides in wheat. The visual limit of detection (vLOD) for PUs including diuron, chlortoluron, neburon, chlorbromuron, and linuron was 1–2 µg/kg. The vLOD for SUs including metsulfuron methyl, ethametsulfuron-methyl, sulfometuron-methyl, tribenuron methyl, cinosulfuron, triasulfuron, chlorimuron-ethyl, and chlorsulfuron was 2–10 µg/kg. The results of real sample determination indicated that the multi-CGIA is accurate, stable, and reliable, and adaptable to on-site preliminary screening of actual samples.
研究领域:
分析化学
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环境科学
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农业科学
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产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2022
·
IF 10.753
Jianan Liu,Jinjin Cheng,Chunli Zhou,Liya Ma,Xiaolong Chen,Yong Li,Xing Sun,Xiaolong Yan,Renhua Geng,Qun Wan,Xiangyang Yu
Food safety problems caused by pesticide residues have always been a concern for many people. In this study, we investigated the uptake, translocation and subcellular distribution of neonicotinoid insecticides, triazole fungicides, and sulfonylurea herbicides in rice plants ( Oryza sativa L.). The time-dependent uptake kinetics of the three categories of pesticides with different molecular structures fit a first-order one-compartment kinetic model. The neonicotinoids (log K ow −0.66–0.8) were mainly concentrated in the leaves, and the triazoles (log K ow 3.72–4.4) were mainly concentrated in the roots. Neonicotinoid pesticides in the roots were preferentially transported across the membrane through the symplastic pathway; triazole pesticides except for triadimefon and myclobutanil preferentially passed through the symplastic pathway; and sulfonylurea pesticides (log K ow 0.034–2.89) were first transported upward through the apoplastic pathway. In the roots, neonicotinoids, triazoles, and sulfonylurea herbicides were mainly concentrated in the soluble fractions, cell wall and apoplast fractions, respectively. In addition, there was a high positive correlation between the subcellular distribution of pesticides in the roots, stems and leaves. Molecular weight and log K ow jointly affected the enrichment of triazole pesticides in the roots, stems and leaves and the transfer from stems to leaves, while water solubility and log K ow commonly affected neonicotinoids. There was a correlation between pesticide absorption and the molecular structures of pesticides. To develop pesticides with strong uptake and transport capabilities, it is necessary to consider that the electronegativity of some atoms is stronger, the sum of the topological indices of heteroatoms can be large, and the van der Waals volume increases accordingly.
研究领域:
农业科学
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植物生理学
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环境毒理学
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农药化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2022
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IF 9.231
Ting Zhao,Xiaochen Liang,Xinjie Guo,Xingjie Yang,Jialiang Guo,Xia Zhou,Xueqin Huang,Wenqian Zhang,Yiqing Wang,Zhaowei Liu,Zhengjin Jiang,Hongke Zhou,Haibo Zhou
A simple, sensitive method for pesticide distinguishment based on a colorimetric sensor array using diverse gold nanoparticles (AuNPs) at room temperature is presented in this study. Acetylcholinesterase (AChE) hydrolysis ability was influenced by different pesticides and produced different concentrations of thiocholine by hydrolyzing acetylthiocholine iodide (ATCh). Thiocholine could be easily linked to the AuNPs through an Au − S covalent bond, and AuNPs underwent aggregation, resulting in a visible color change due to alteration of surface plasmon resonance properties. Based on these results, we successfully distinguished eight pesticides (glyphosate, thiram, imidacloprid, tribenuron methyl, nicosulfuron, thifensulfuron methyl, dichlorprop, and fenoprop) utilizing five different AuNPs by colorimetric assay. The limit of detection (LOD) of this visual method for all pesticides was less than 1.5 × 10 -7 M, which was more sensitive than the U.S. Environmental Protection Agency regulations specify (1.18 ∼ 3.91 × 10 -6 M). This method was further improved by combining a portable smartphone device with a color picking application using (color name AR) and RGB (red, green, blue) values. The method was successfully applied to pesticide residue distinguishment in real samples by linear discriminant analysis (LDA).
研究领域:
分析化学
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环境科学
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纳米技术
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食品安全
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）