JOURNAL OF CONTROLLED RELEASE
·
2017
·
IF 7.786
Jingjing Hu,Yanchun Quan,Yuping Lai,Zhao Zheng,Zhiqi Hu,Xinyu Wang,Tianjiao Dai,Qiang Zhang,Yiyun Cheng
Aminoglycosides are a family of critically important antibiotics for the treatment of serious infections including multidrug-resistant pathogens. However, clinical use of aminoglycoside antibiotics is compromised by bacterial biofilm formation at subinhibitory concentrations or adverse side effects such as ototoxicity and nephrotoxicity at high antibiotic doses. Preparation of aminoglycoside formulation that allows on-demand drug delivery is a solution to this sticky issue. Here, we designed a new type of aminoglycoside hydrogels by cross-linking oxidized polysaccharides such as dextran , carboxymethyl cellulose , alginate , and chondroitin using aminoglycosides as cross-linkers. The hydrogel modulus, degradation rate and release kinetics can be precisely modulated by tailoring the aminoglycoside dose during gel formation. The aminoglycoside hydrogel showed fast gelation , self-healing and on-demand drug release behaviors, and high antibacterial activities in vitro and in vivo against both Gram-positive and Gram-negative bacteria. This study provides a facile and promising strategy to develop smart hydrogels for topical administration of aminoglycoside antibiotics.
研究领域:
药学
·
生物材料
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NANO
·
2013
·
IF 1.167
RUIYONG WANG,SHUMIN FAN,RUIQIANG WANG,RUI WANG,HUANJING DOU,LVJING WANG
A sensitive and selective colorimetric biosensor for determination of gentamicin, amikacin and tobramycin was proposed with the unmodified gold nanoparticles (GNPs) as the sensing element. Gentamicin, amikacin and tobramycin can rapidly induce the aggregation of gold nanoparticles and is accompanied by a color change from red to blue. The concentration of gentamicin, amikacin and tobramycin can be determined by using UV-Vis spectrometer. The experimental parameters were optimized with regard to pH, incubation time and the concentration of the GNPs. Under optimal experimental conditions, the linear range of the colorimetric sensor for gentamicin/amikacin/tobramycin were 2.67–33.93 ng mL -1 , 13.33–66.67 ng mL -1 and 20–180 ng mL -1 , respectively. The corresponding limit of detection (3σ) was 0.354 ng mL -1 , 0.999 ng mL -1 and 0.579 ng mL -1 , respectively. This assay was simple and used to detect aminoglycoside antibiotics in milk and medicine products.
研究领域:
分析化学
·
生物传感
·
药学
✓ 已验证
产品关联
DOI
高影响因子
WATER AIR AND SOIL POLLUTION
·
2024
·Vol. 235
·期 6
·1-12
·
IF 3.8
Shi Yanfeng,Li Bingxu,Shi Yuzhi,Sun Yuanyuan,Ding Botao,Zhang Yanhao,Zhang Xu,Zhang Zhibin,Li Fulin
The antiviral drug ribavirin is widely used and continuously released into the environment through various pathways, which will cause serious water pollution and virus resistance. However, only few works have examined ribavirin retention and transport groundwater environment. This study investigates the transport behavior of ribavirin in saturated quartz sand and limestone porous media, considering ionic strength (IS), ionic type and humic acid (HA), through column experiments and mathematical modeling. The findings reveal that, ribavirin exhibits strong mobility within saturated quartz sand media is less affected by different hydrochemical factors, posing a high risk of migration. In limestone porous media, the migration of ribavirin decreased. In comparison to Na + , the presence of Ca 2+ causes the effluent recovery rate of ribavirin decreases. And as the IS of Ca 2+ background solution increases, the effluent recovery rate of ribavirin also decreases. The presence of HA causes more ribavirin to flow out of the column, and its effect becomes more pronounced with increasing concentration. Furthermore, simulated precipitation scenarios, the ribavirin adsorbed on the of media surface is released again and transport with the water flow. These findings enhance our understanding about the environmental fate of ribavirin and facilitating ecological restoration efforts.
研究领域:
环境科学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Guoxu Ao,Zhaoxuan Wang,Yueqi Shi,Hongzhi Ling,Shanshan Sun,Wenxiang Ping
Efficient elimination of antibiotics, particularly aminoglycosides, and Antibiotic resistance genes (ARGs) from agricultural and livestock waste, as well as enhancing the level of humification, remains a major challenge in the pursuit of high-value utilization of agricultural waste. Here, the roles of bacteria and fungus in the dissipation of aminoglycoside antibiotics, ARGs and the enhancement of humification with the addition of humic acid (HA) to compost systems were investigated. Compared to the control, the contents of humus (HS) and HA were increased by 41.3 and 143.3 %, respectively, and the content of fulvic acid (FA) was decreased by 74.1 % with HA addition. Interestingly, the abundance of aminoglycoside ARGs was decreased significantly ( p < 0.05) as well as the abundance of functional genes involved in oxidative stress response and type IV secretion system (T4SS). The bacterium Nocardiopsis related to carbon cycle, and the fungi Ascomycota and Basidiomycota associated with ligocellulose degradation were significantly enriched ( p < 0.05). Structural equation models revealed that the decrease in aminoglycoside ARGs abundance was attributed to bacteria promoting HA synthesis, thereby inhibiting the multiplication of aminoglycoside ARGs and ultimately mitigating oxidative damage to microbial cells. These findings shed light on the mechanism by which HA removes aminoglycoside ARGs, and provide valuable insights into enhancing the biosafety of compost products.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2019
·Vol. 25
·期 1
·100
·
IF 3.06
Gaofei Liu,Tiantong Cheng,Jianlin Chu,Sui Li,Bingfang He
Purine nucleoside phosphorylases (PNPs) are promising biocatalysts for the synthesis of purine nucleoside analogs. Although a number of PNPs have been reported, the development of highly efficient enzymes for industrial applications is still in high demand. Herein, a new trimeric purine nucleoside phosphorylase (AmPNP) fromAneurinibacillus migulanusAM007 was cloned and heterologously expressed inEscherichia coliBL21(DE3). TheAmPNP showed good thermostability and a broad range of pH stability. The enzyme was thermostable below 55 °C for 12 h (retaining nearly 100% of its initial activity), and retained nearly 100% of the initial activity in alkaline buffer systems (pH 7.0–9.0) at 60 °C for 2 h. Then, a one-pot, two-enzyme mode of transglycosylation reaction was successfully constructed by combining pyrimidine nucleoside phosphorylase (BbPyNP) derived fromBrevibacillus borstelensisLK01 andAmPNP for the production of purine nucleoside analogs. Conversions of 2,6-diaminopurine ribonucleoside (1), 2-amino-6-chloropurine ribonucleoside (2), and 6-thioguanine ribonucleoside (3) synthesized still reached >90% on the higher concentrations of substrates (pentofuranosyl donor: purine base; 20:10 mM) with a low enzyme ratio ofBbPyNP:AmPNP (2:20 μg/mL). Thus, the new trimericAmPNP is a promising biocatalyst for industrial production of purine nucleoside analogs.Keywords:trimeric nucleoside phosphorylase;thermostability;purine nucleoside analogs;enzyme ratio
研究领域:
生物化学
·
生物技术
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Dan Wu,Peng Liu,Wenjia Fan,Ke Shi,Xi Luo,Feng Zheng,Shunli Ji
Bio-based and low-cost hybrid polyvinyl alcohol (PVA) and gelatin (Gel) hydrophilic macromolecular complex coated microspheres were prepared based on one-pot process, characterized, and applied as novel sorbent materials for the purification of trace aminoglycosides from complex matrices. PVA acts as a "rigid" component in the hybrid complex to enhance its mechanical properties, while Gel's "flexible" role is to improve the swelling properties of the hybrid complex in water. It is shown that hybrid PVA/Gel-functionalized sorbents are more efficient than the respective PVA or Gel sorbents since the presence of Gel increases the material selectivity for aminoglycosides, which is due to the specific interactions occurring between the targets and amino acid residues in the hybrid materials. Under the optimum conditions, material preparation and pretreatment processes were entirely carried out in single water system without toxic organic solvent. The detection limit (LOD) of spectinomycin, kanamycin, streptomycin and dihydrostreptomycin in honey were 0.811, 0.303, 0.168, 0.045 μg⋅kg −1 respectively. Linearity was obtained in the range of 20 to 2000 ug⋅kg −1 , relative recovery yield up to 84.1-111.7% were obtained and matrix effect of all four aminoglycosides was within 100.8-107.6%. Intra-day and inter-day precision under four spiking levels (5, 200, 500 and 1000 ug⋅kg −1 ) were less than 10.9% (n=6) and 13.6% (n=3) respectively. In addition, the sorbents exhibited excellent reusability even after six recycles. This work demonstrates the potential of bio-based and low-cost hybrid polymer extraction platforms as promising bonded phase alternatives, in which eco-friendly and natural-based polymers can be used to improve the material selectivity and are conducive to the realization of "green chemistry".
研究领域:
分析化学
·
环境科学
·
生物材料
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Shiyao Hua,Jiayue Liu,Sijia Pu,Liang Zou,Lei Zhang,Peng Li
Comprehensive elucidation of the chemical constituents of Astragali Radix is crucial for its quality control and edible product development. Herein, online comprehensive two-dimensional hydrophilic interaction liquid chromatography × reversed phase liquid chromatography tandem high-resolution mass spectrometry was elaborately designed to separate, identify, and qualitatively compare various chemical components of Astragali Radix from two species. After selection and optimization of stationary phase, mobile phase, elution program and interface, an optimum method suitable for chemical components in Astragali Radix was established successfully. Under the optimized separation conditions, the orthogonality reached 99 %. A total of 212 compounds were identified and tentatively identified (198 from A. membranaceus var. mongholicus and 177 from A. membranaceus ). Additionally, the fragmentation pattern of flavonoids and saponins mass data in Astragali Radix have also been systematically studied. These findings not only unveiled multi-component complexity and diversity in two Astragalus species, but also proposed an innovative strategy for the rapid separation and identification of other complex systems.
研究领域:
中药学
·
分析化学
·
植物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
·
2015
·
IF 3.564
Xue-Jie Wang,Jin-Zong You
The thermal decomposition processes of eight purine nucleoside analogs were measured with thermogravimetry (TG) and differential scanning calorimetry (DSC). The IR spectra and high-performance liquid chromatography (HPLC) of purine nucleoside analogs and their residues from thermal decomposition at various temperatures were determined. The molecular bond orders were calculated with an ab initio method from the GAMESS program. The mechanisms of thermal decomposition for purine nucleoside analogs were discussed. The results indicate that the main decomposition pathway of purine nucleoside analogs is the fracture of the N -glycosidic bond first, and the residues of the initial stage of decomposition are mainly corresponding purines. A part of purine nucleoside analogs decomposes directly to small molecules and insoluble substances at the first stage. When the N -glycosidic bond order is close to that of the weakest bond within purine ring, most of purine nucleoside analogs will decompose directly. The amino group at purine ring is easy to be oxidized, some oxidation products can be found in the residues of some purine nucleoside analogs. There is a positive correlation between the calculated weakest bond orders and the decomposition temperatures of purine nucleoside analogs. The stronger the weakest bond order, the higher the decomposition temperature. The molecular bond orders can serve as the basis to judge molecular thermal stability for analog compounds with similar molecular structure, size, and energy. The substituent group affects the thermal stability of the purine nucleoside analogs. Increasing the number of electron-donating groups on the purine ring and furanose ring will enhance the N -glycosidic bond, will increase the temperature of thermal decomposition, and be likely to change the mechanism of thermal decomposition.
研究领域:
生物化学
·
药物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF FLUORESCENCE
·
2015
·Vol. 25
·期 2
·451-463
·
IF 1.927
Li Zhen,Li Zhigang,Yang Lingling,Xie Yuanzhe,Shi Jie,Wang Ruiyong,Chang Junbiao
In this paper, the interactions of pepsin with CYD (cytidine) or nucleoside analogs, including FNC (2′-deoxy-2′-β-fluoro-4′-azidocytidine) and CMP (cytidine monophosphate), were investigated by fluorescence, UV–visible absorption and synchronous fluorescence spectroscopy under mimic physiological conditions. The results indicated that FNC (CYD/CMP) caused the fluorescence quenching by the formation of complex. The binding constants and thermo-dynamic parameters at three different temperatures were obtained. The hydrophobic and electrostatic interactions were the predominant intermolecular forces to stabilize the complex. The F atom in FNC might weaken the binding of nucleoside analog to pepsin. Results showed that CYD was the strongest quencher and bound to pepsin with higher affinity.
研究领域:
生物化学
·
结构生物学
·
药物设计
·
分子诊断
·
酶学
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2018
·Vol. 25
·期 17
·17128-17136
·
IF 2.8
Tan Lin-Rui,Xia Peng-Fei,Zeng Raymond J.,Li Qian,Sun Xue-Fei,Wang Shu-Guang
The interactions between antibiotics and microorganisms have attracted enormous research attentions. In this study, we investigated the effects of two typical aminoglycoside antibiotics on the aggregation of the model cyanobacterium, Synechococcus elongatus , and the dominating strain in algal blooms, Microcystis aeruginosa , via the analysis of zeta potentials, hydrophobicity, and extracellular polymeric substances (EPS) secretion . The results showed that low-level antibiotics promoted the aggregation of S. elongatus and M. aeruginosa by 40 and 18% under 0.10 and 0.02 μg/mL of kanamycin, respectively, which was mainly attributed to the combined effects of increased zeta potentials and the ratio between extracellular proteins and polysaccharides. Tobramycin exerted similar effects. Additionally, we discovered that at low pH (pH 5) and ionic strength (1 mM Na + and 2 mM Mg 2+ ), the inducing effects of antibiotics would be even larger than those with higher pH and ionic strength. As aggregation is important to cyanobacteria in either the basic physiology of biofilm formation or the algal bloom, our study demonstrated that low-level antibiotics exert ecological impacts via interfered aggregation. We believe this study will shed light on the mechanisms underlying antibiotic-induced biofilm formation and help with the evaluation of the environmental and ecological risks of antibiotics and other emerging pollutants.
研究领域:
环境科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHARMACOLOGICAL RESEARCH
·
2020
·
IF 5.893
Ruocong Yang,Hao Liu,Chen Bai,Yingchao Wang,Xiaohui Zhang,Rui Guo,Siying Wu,Jianxun Wang,Elaine Leung,Hang Chang,Peng Li,Tiegang Liu,Yi Wang
The Coronavirus Disease 2019 (COVID-19) pandemic has become a huge threaten to global health, which raise urgent demand of developing efficient therapeutic strategy. The aim of the present study is to dissect the chemical composition and the pharmacological mechanism of Qingfei Paidu Decoction (QFPD), a clinically used Chinese medicine for treating COVID-19 patients in China. Through comprehensive analysis by liquid chromatography coupled with high resolution mass spectrometry (MS), a total of 129 compounds of QFPD were putatively identified. We also constructed molecular networking of mass spectrometry data to classify these compounds into 14 main clusters, in which exhibited specific patterns of flavonoids (45 %), glycosides (15 %), carboxylic acids (10 %), and saponins (5 %). The target network model of QFPD, established by predicting and collecting the targets of identified compounds, indicated a pivotal role of Ma Xing Shi Gan Decoction (MXSG) in the therapeutic efficacy of QFPD. Supportively, through transcriptomic analysis of gene expression after MXSG administration in rat model of LPS-induced pneumonia, the thrombin and Toll-like receptor (TLR) signaling pathway were suggested to be essential pathways for MXSG mediated anti-inflammatory effects. Besides, changes in content of major compounds in MXSG during decoction were found by the chemical analysis. We also validate that one major compound in MXSG, i.e. glycyrrhizic acid, inhibited TLR agonists induced IL-6 production in macrophage. In conclusion, the integration of in silico and experimental results indicated that the therapeutic effects of QFPD against COVID-19 may be attributed to the anti-inflammatory effects of MXSG, which supports the rationality of the compatibility of TCM.
研究领域:
药理学
·
医学
·
中医药
·
生物信息学
·
分子生物学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2024
·
IF 3.1
Jingru Song,Yanping Huang,Lu Liu,Dengcheng Hui,Zheng Wang,Dong Xie,Yulang Jiang,Hongyan Cao,Yancheng Dai,Guan Ye,Shibing Su,Mingmei Zhou,Qin Zhang,Mingyu Sun
Yinchenhao decoction (YCHD) is widely used in the treatment of damp-heat syndrome of chronic hepatitis B (CHB), but it remains unclear about the active compounds in YCHD and its potential mechanism for treating CHB. The purpose of this work is to evaluate the clinical efficacy of YCHD combined with nucleoside analogues (NAs) for the treatment of CHB. Besides, based on the exact clinical efficacy, we combined serum metabolomics and network pharmacology to screen differential metabolites and related pathways regulated by YCHD to investigate the possible mechanism for treating CHB. It revealed that NAs plus YCHD could significantly improve alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, increase HBV-DNA negative rate ( P <0.05), reduce the levels of inflammatory factors and LSM (both P <0.05), regulate lipids ( P <0.05), and improve the symptoms of traditional Chinese medicine (TCM) ( P <0.05) in CHB patients. YCHD was relatively safe. It showed 30 active compounds including chlorogenic acid, geniposide, emodin, quercetin, kaempferol, β-sitosterol and aloe emodin, and 115 key targets which were related to the regulation of lipids and reduction of oxidative stress related to the effect of YCHD in CHB in the network pharmacology analysis. We found 9 core targets and 4 key metabolites according to metabolomics, which were partly consistent with the network pharmacology findings. It proved that network pharmacology combined with metabolomics can well explain the “multi-component-multi-target” mechanism of complex TCM.
研究领域:
中药学
·
肝脏病学
·
代谢组学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）