SEPARATION AND PURIFICATION TECHNOLOGY
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2024
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IF 8.1
Ruimiao Chang,Huichun Wang,Shumian Liu,Yijing Dong,Guangbin Zhang,Anjia Chen,Ruiping Zhang,Yong Li
For rapid separation of quercetin from apple peel, a novel magnetic nanomaterial was prepared by a simple and economical thermal alkaline hydrolysis method in this study. This magnetic nanomaterial was obtained by mixing and then heating magUiO-66-NH 2 (magnetic University of Oslo 66-NH 2 ) with 0.05 mol·L −1 KOH aqueous solution, and was named magUiO-66-NH 2 -MH 0.05 (magUiO-66-NH 2 -mixed heating 0.05 ), which has better magnetic properties, thermal stability, pore capacity and average pore size than magUiO-66-NH 2 . The obtained magUiO-66-NH 2 -MH 0.05 had strong adsorption capacity for quercetin, and the maximum adsorption capacity was 65.30 mg·g −1 . MagUiO-66-NH 2 -MH 0.05 tends to be monolayer-layer adsorption for quercetin, and electron sharing or transfer may occur during the adsorption process. Compared with naringenin, naringin and gallic acid, magUiO-66-NH 2 -MH 0.05 showed good recognition ability for quercetin, and the recovery rate was good. After 7 cycles, the removal rate of magUiO-66-NH 2 -MH 0.05 for quercetin was 96.3 % of the initial adsorption capacity. A magnetic solid-phase extraction method coupled with High-Performance Liquid Chromatography based on magUiO-66-NH 2 -MH 0.05 was successfully used for rapid separation and determination of quercetin in apple peel. This study will help to discover the experimental law of the treatment of UiO-66-NH 2 nanomaterials by hot alkaline hydrolysis, reveal the reaction mechanism, and expand the application of UiO-66-NH 2 nanomaterials in food analysis.
研究领域:
食品科学
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分析化学
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产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2023
·
IF 4.1
Wen Xia,Bingbing Song,Tong Li,Rui Hai Liu
BACKGROUND Blueberries and apples exhibit favorable bioactivity and health benefits as a result of their rich phytochemicals. Natural phytochemicals exist in complex forms, but there are few reports on whether have additive, synergistic or antagonistic effects between different phytochemicals. The present study aimed to elucidate the synergistic effects of blueberry extract (BE) and apple peel extract (APE) together with respect to inhibiting the proliferation of HepG2 liver cancer cells. Meanwhile, phytochemical characterization of BE and APE was conducted by HPLC, and total antioxidant activity was determined via a cellular antioxidant activity assay, oxygen radical absorption capacity assay and peroxy radical scavenging capacity assay. RESULTS The results showed that BE and APE were rich in phytochemicals and had potent antioxidant activities, which synergistically inhibited cell proliferation. In the bilateral combination, the dose reduction index value increased by two-fold, and the combination index value at 95% inhibition was less than 1. Additionally, BE + APE supplementation could promote the expression levels of p53 and c-myc genes. In conclusion, the BE and APE had strong antioxidant activity and exhibited synergistic inhibition against proliferation of HepG2 cells. CONCLUSION The present study can provide a theoretical basis for the synergistic effect of different phytochemicals in health care. © 2023 Society of Chemical Industry.
研究领域:
植物化学
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肿瘤学
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营养学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
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2017
·Vol. 40
·期 8
·1651-1659
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IF 2.557
Tong Wang,Hai-Long Wu,Li-Xia Xie,Li Zhu,Zhi Liu,Xiao-Dong Sun,Rong Xiao,Ru-Qin Yu
In this work, a smart chemometrics-enhanced strategy, high-performance liquid chromatography, and diode array detection coupled with second-order calibration method based on alternating trilinear decomposition algorithm was proposed to simultaneously quantify 12 polyphenols in different kinds of apple peel and pulp samples. The proposed strategy proved to be a powerful tool to solve the problems of coelution, unknown interferences, and chromatographic shifts in the process of high-performance liquid chromatography analysis, making it possible for the determination of 12 polyphenols in complex apple matrices within 10 min under simple conditions of elution. The average recoveries with standard deviations, and figures of merit including sensitivity, selectivity, limit of detection, and limit of quantitation were calculated to validate the accuracy of the proposed method. Compared to the quantitative analysis results from the classic high-performance liquid chromatography method, the statistical and graphical analysis showed that our proposed strategy obtained more reliable results. All results indicated that our proposed method used in the quantitative analysis of apple polyphenols was an accurate, fast, universal, simple, and green one, and it was expected to be developed as an attractive alternative method for simultaneous determination of multitargeted analytes in complex matrices.
研究领域:
食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Foods
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2023
·Vol. 12
·期 2
·310
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IF 5.2
Haiyan Li,Yugang Liu,Shiyin Guo,Meng Shi,Si Qin,Chaoxi Zeng
Extracting ursolic acid (UA) from plant resources using organic solvents is incompatible with food applications. To address this, in this study, 15 edible hydrophobic deep eutectic solvents (HDESs) were prepared to extract UA from apple peel, the extraction conditions were optimized, and the optimization strategies were compared. It was found that the solubility of UA in the HDESs can be 9 times higher than the traditional solvent such as ethanol. The response surface optimization concluded that temperature had the greatest effect on the extraction and the optimized test conditions obtained as follows: temperature of 49 °C, time of 32 min, solid–liquid ratio of 1:16.5 g/mL, respectively. Comparing the response surface methodology (RSM) and artificial neural networks (ANN), it was concluded that ANN has more accurate prediction ability than RSM. Overall, the HDESs are more effective and environmentally friendly than conventional organic solvents to extract UA. The results of this study will facilitate the further exploration of HDES in various food and pharmaceutical applications.Keywords:apple peel;ultrasonic-assisted extraction;response surface methodology;artificial neural network
研究领域:
食品科学
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药学
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化学工程
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生物技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2024
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IF 3.3
Wanyu Yan,Cong Chen,Yuanru Zheng,Jingwen Xu,Yihai Wang,Xiangjiu He
BACKGROUND Apples are among the most nutritionally valuable fruits and have a history of use in traditional Chinese medicine. Triterpenoids, the primary bioactive compounds found in apples, demonstrate significant antitumor activity. RESULTS Following enrichment and optimization, the total content of major triterpenoids in total triterpenoids from apple peels (ATT) reached 5.76 g kg −1 . The growth of MDA-MB-231 xenograft tumors was significantly inhibited after treatment with ATT. Network pharmacology analysis conclusively identified a close association between the antitumor effect of ATT and the phosphatidylinositol 3-kinase–protein kinase B (PI3K-Akt) signaling pathway. Experimental validation using MDA-MB-231 cells and a xenograft nude mouse model confirmed that ATT suppressed tumor cell proliferation effectively by modulating the PI3K-Akt signaling pathway, which was consistent with the findings from network pharmacology. The total triterpenoids from apple peels also induced cell apoptosis by mediating the PI3K-Akt signaling pathway. CONCLUSION The total triterpenoids from apple peels can inhibit tumor cell proliferation and induce cell apoptosis effectively through the PI3K-Akt signaling pathway, suggesting that ATT holds promise as a prospective therapeutic agent for breast cancer treatment. © 2024 Society of Chemical Industry.
研究领域:
药理学
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肿瘤学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2025
·
IF 9.8
Muhammad Nawaz,Hafiz Muhammad Rizwan,Zia Ud Din Momand,Irfan Ullah,Jiayi He,Qian Zhou,Mingfu Wang
Apple peel, a byproduct of apple processing or consumption, is often discarded, contributing to environmental pollution and a considerable loss of valuable bioresources. This study explored the health-promoting potential of apple peel through fermentation with Lactobacillus plantarum ATCC14917. Our previous study indicated that this strain could increase the in vitro antioxidant activity of apple juice. In this study, we examined the phytochemical changes of apple peel extract (APe) fermented by Lactobacillus plantarum ATCC14917, as well as evaluating its beneficial effects against advanced glycation end products (AGEs)-induced cellular responses. In particular, the primary phytochemicals were identified as chlorogenic acid, rutin, isoquercitrin, quercetin-3-xyloside, and quercitrin in APe. After fermentation by L. plantarum ATCC14917, APe exhibited changes in phytochemical content, as well as induced pronounced anti-inflammatory and antioxidant activities. Notably, fermentation increased the levels of quercitrin and quercetin-3-xyloside, while slightly decreased the levels of chlorogenic acid, rutin, and isoquercitrin. The highest antioxidant activities were observed in APe at a concentration of 2.0 mg/mL after 72 h of fermentation (F-APe). Furthermore, F-APe significantly reduced the ROS level and inflammatory responses in RAW 264.7 cells by modulating RAGE/MAPKs/NF-κB signaling pathway, demonstrating enhanced potentials compared to non-fermented APe (NF-APe). Collectively, our findings provide the first evidence that L. plantarum ATCC14917 fermentation specifically converts apple peel waste into bioactive compounds with enhanced beneficial effects, offering a novel strategy for the prevention and management of diseases linked to AGEs.
研究领域:
食品科学
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微生物学
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天然产物化学
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分子营养学
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代谢疾病
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功能食品
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生物转化
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产品关联
DOI
PubMed
高影响因子
CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS
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2018
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IF 2.701
Xue-Qin Zhang,Sheng-Xian Li,Zeng-Ping Chen,Yao Chen,Ru-Qin Yu
Due to the practical impossibility in controlling the number and distribution of "hot spots" on or close to the surfaces of enhancing substrates, internal standards are generally used to improve the accuracy and precision of quantitative surface-enhanced Raman spectroscopy (SERS). Traditional internal standard methods based on either univariate ratiometric models or conventional multivariate calibration models can use the information of only one characteristic SERS peak of only one internal standard, which weakens the efficiency of traditional internal standard methods in improving the quantitative results of SERS assays. In this contribution, the concept of using multiple internal standards was introduced for the first time to quantitative SERS assays by incorporating a unique spectral shape deformation (SSD) quantitative theory with multiple-internal-standard embedded core-shell nanoparticles . The quantification of phosmet residues on apple skins by SERS technique with Au-core/Ag-shell nanoparticles embedded with both 2-MB and PATP as enhancing substrate was used to illustrate the applicability of the proposed strategy. Experimental results demonstrated that the proposed multiple-internal-standard strategy based on SSD achieved much more accurate and precise concentration predictions for phosmet residues on apple skin than traditional single-internal-standard methods based on either univariate ratiometric calibration models or conventional multivariate calibration models. The SERS quantitative results of the multiple-internal-standard strategy were comparable to those obtained by LC-MS/MS. The average relative deviation between the quantitative results of the proposed method and those of ICP-MS was about 8%. The good accuracy of the proposed method makes it a promising alternative for quantitative SERS assays.
研究领域:
分析化学
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食品科学
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纳米技术
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DOI
高影响因子
Innovative Food Science & Emerging Technologies
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2025
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IF 6.8
Huizhong Hu,Peijian Cai,Yi Liu,Yufu Li,Jinhong Zhong,Fansheng Kong
Apple peel polyphenols (APPs) have high bioactivity, but are susceptible to pH and temperature changes and have low bioavailability. In this study, liposomes encapsulating APPs were prepared from reverse evaporation, and surface-modified with chitosan (CS) and sodium alginate (SA) to enhance the physicochemical stability, gastrointestinal stability, and bioactivity of APPs. Liposomes were characterized using DLS, FTIR, SEM, and TEM. After layer-by-layer modification, the particle size of the liposomes increased from 267.03 ± 1.04 to 421.86 ± 3.84 nm. The encapsulation rate increased from 86.52 ± 0.60 to 92.04 ± 0.13, and polydispersity index was 0.29 ± 0.01. Double-layered modified liposomes exhibit excellent encapsulation efficiency and form nanoscale spheres. SA-CS-APPL demonstrates significantly enhanced in vitro antioxidant, hypoglycemic, and antibacterial activities compared to CS-APPL and APPL. SA-CS-APPL exhibits superior physicochemical stability over CS-APPL and APPL under various storage conditions, pHs, and temperatures. In vitro digestion experiments indicate SA-CS-APPL exhibits higher stability and a slower release rate of APPs compared to CS-APPL and APPL. The bioavailability of SA-CS-APPL is 39.63 %, representing a 28.28 % increase compared to free APPs. Therefore, SA-CS double-layered modified nanoliposomes can serve as a potential nanocarrier for APPs, aiding in the development of more effective bioactive substance release systems.
研究领域:
食品科学
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纳米技术
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药剂学
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生物技术
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生物化学
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产品关联
DOI
高影响因子
Food & Function
·
2023
·Vol. 14
·期 14
·6678-6689
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IF 6.1
Ziqian He,Na Deng,Bisheng Zheng,Yingjie Gu,Jian Chen,Tong Li,Rui Hai Liu,Ling Yuan,Wenzhi Li
The intestine and its flora have established a strong link with each other and co-evolved to become a micro-ecological system that plays an important role in human health. Plant polyphenols have attracted a great deal of attention as potential interventions to regulate the intestinal microecology. In this study, we investigated the effects of apple peel polyphenol (APP) on the intestinal ecology by establishing an intestinal ecological dysregulation model using lincomycin hydrochloride-induced Balb/c mice. The results showed that APP enhanced the mechanical barrier function of mice by upregulating the expression of the tight junction proteins at the transcriptional and translational levels. In terms of the immune barrier, APP downregulated the protein and mRNA expression of TLR4 and NF-κB. As for the biological barrier, APP promoted the growth of beneficial bacteria as well as increasing the diversity of intestinal flora. In addition, APP treatment significantly increased the contents of short-chain fatty acids in mice. In conclusion, APP can alleviate intestinal inflammation and epithelial damage as well as inducing potentially beneficial changes in the intestinal microbiota, which helps to reveal the potential mechanisms of host–microbial interactions and polyphenol regulation of intestinal ecology.
研究领域:
药理学
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微生物学
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免疫学
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营养学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）