JOURNAL OF APPLIED POLYMER SCIENCE
·
2019
·Vol. 136
·期 45
·48186
·
IF 2.188
Xiaoguang Ying,Anshun Kang,Xiaomei Zhu,Xiao Li
Electrospinning is an important tool for generating continuous fibers with large surface area and high pore ratio. The combination of nanofiber membrane and molecular imprinting can effectively measure specific adsorption of putrescine with coloring procedure. In this work, molecularly imprinted electrospun chromogenic membrane was synthesized with 1,4-butanediol (BDO) as imitate template. Poly(vinyl alcohol) (PVA) is used as spinning solution and ninhydrin as chromogenic agent for visualizing detection of target molecular putrescine. The adsorption concentration on molecularly imprinted polymer is illustrated by blue-purple colored stains and recorded by interactive three-dimensional surface plot of ImageJ. The results indicated that a uniform and bead-free PVA nanofiber was obtained using 10% (w/w) PVA solution, 15 kV voltage, 15 cm spinning distance, and 600 rpm roller speed. Also, the adsorption performance of molecular imprinting chromogenic membrane was investigated in detail, showing a higher adsorption capacity of 33.16 μg cm −2 and maximum imprinting efficiency of 2.30 under the best recognition conditions of 315 μL ninhydrin, 0.25% (w/w) BDO, and 0.4 cm sensing distance, as well as a short adsorption equilibrium time of 40 min. In addition, three amine competitors were discussed to reveal the better selectivity and higher specific recognition ability in the imprinted membrane. HIGHLIGHTS The molecular imprinting and coloring structure is constructed in nanofiber membrane with an enhanced specific surface area. The adsorption on imprinted membrane is visualized by chromogenic reaction between putrescine and ninhydrin to form a three-dimensional interactive surface proceeded using the ImageJ software. The imprinted nanofiber membrane with large area and high pore ratio is applied in detecting target in trace concentration within shortened time. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136 , 48186.
研究领域:
材料科学
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分析化学
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纳米技术
·
高分子科学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2013
·
IF 3.498
Jiao Li,Yuanyuan Li,Tong Chen,Luan Xu,Xiaoyan Liu,Xiaoli Zhang,Haixia Zhang
In this paper, linear and cyclic tetrapeptide bonded stationary phases, were synthesized by the solid-phase synthesis method. Each amino acid coupling step was monitored by the ninhydrin test using UV/visible spectrophotometry. The proposed materials were characterized by UV spectroscopy , Fourier transform infrared spectroscopy, elemental analysis and thermogravimetric analysis , which proved the successful immobilization of tetrapeptide on the silica support. The cyclopeptide stationary phase displayed mixed-mode behavior and had the size selectivity for polycyclic aromatic hydrocarbon such as anthracene , while the linear peptide stationary phase only exhibited typical hydrophilic interaction chromatography (HILIC) characteristics and was more hydrophilic under the same mobile phase condition. Retention behaviors of polar compounds on the two stationary phases were studied through varying column temperature, the water content, pH and ionic strength in mobile phase. Applications in the separation of the mixture of nucleosides , sulfa compounds, organic acids and isomers of substituted benzoic acid compounds were demonstrated.
研究领域:
化学
·
生物学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
·
2025
·
IF 3
Yilin Huang,Xingli Huo,Huijun Liu,Danni Li,Zongning Yin
Lysine succinylation is a major post-translational modification affecting diverse proteins, and its excessive occurrence can lead to protein misfolding and aggregation—hallmarks of various proteinopathies, such as amyloid-β and Tau tangle formation in Alzheimer's disease and islet amyloid polypeptide aggregation in type 2 diabetes. Here, we investigated the inhibitory effects of bile acid metabolites (deoxycholic acid, glycocholic acid, and taurocholic acid sodium) and natural polyphenols (anthocyanin and salidroside) on succinylation and succinylation-induced amyloid aggregation. Succinylation levels were evaluated using the ninhydrin assay before and after treatment, and aggregation behavior and structural alterations were characterized by SDS-PAGE, inverted fluorescence microscopy, and intrinsic fluorescence spectroscopy. Additionally, fluorescence quenching and molecular docking were used to explore the underlying mechanisms. All five small molecules significantly reduced succinylation in a concentration-dependent manner (p < 0.05). Among bile acids, taurocholic acid sodium exhibited the strongest suppression of aggregation (40.99 %), followed by glycocholic acid (28.32 %) and deoxycholic acid (27.94 %). Anthocyanin showed greater inhibition (33.81 %) than salidroside (26.54 %) (all p < 0.05). The results suggest that these small molecules inhibit succinylation-induced aggregation potentially by interacting with proteins and altering their conformations, thereby preventing excessive succinylation at lysine residues. This study provides new insight into the interplay between bile acid metabolism and protein homeostasis and highlights the therapeutic potential of natural compounds in preventing protein aggregation-related diseases.
研究领域:
生物化学
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分子生物学
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神经退行性疾病
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代谢
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药理学
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结构生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Liru Zhang,Jie Liu,Xuejing Zheng,Ali Zhang,Xianliang Zhang,Keyong Tang
Herein the construction of a strong gelatin hydrogel is presented by using pullulan dialdehyde (PDA) as a macromolecular crosslinker. The resultant PDA crosslinked gelatin hydrogels (G-PDA) exhibit extremely high mechanical strength, manifested in the achieved optimal compressive stress of 5.80 MPa at 80% strain, which is up to 152 times higher than pure gelatin hydrogel. The G-PDA were characterized by Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). The extent of crosslinking was determined by ninhydrin assay. The results suggested that the synergistic effect of dual-crosslinking, which is composed of short- and long-range covalent crosslinking and thermoreversible physical crosslinking, may played a key role in enhancing the load-bearing capacity of ensuing hydrogels. The swelling and enzymatic degradation of G-PDA are gradually limited with increasing PDA concentration. The result from MTT assay demonstrated that G-PDA is non-cytotoxic against MC3T3 cells, regardless of the concentrations of PDA.
研究领域:
材料科学
·
生物医学工程
·
生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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