Agronomy-Basel
·
2024
·Vol. 14
·期 1
·156
·
IF 3.3
Zuguo Xi,Huiyan Jia,Yifan Li,Jinqing Ma,Mengqian Lu,Zhihui Wang,Dexu Kong,Wei-Wei Deng
Tea is a healthy beverage made from the leaves of the tea plant [Camellia sinensis(L.) O. Kuntze]. The tea plant is a perennial evergreen plant that is widely distributed in tropical and subtropical regions. PR proteins (pathogenesis-related proteins, PRs) are defense-related proteins induced under pathogenesis-related conditions. Currently, there are a few studies on PRs in plants. Tea leaf color mutants have been of wide interest to scientists as special materials. A tea plant displaying variegated spontaneous albinism found in a local tea plantation in Huangshan City, Anhui Province, China, was used as the material sample in this study. An analysis of preexisting transcriptomic and proteomic data revealed the significant upregulation of three classes of PRs. In order to investigate the correlation between PRs and variegated tea plants, a series of studies were conducted onPRgenes. The results revealed the identification of 17CsPR1, 3CsPR4and 31CsPR5genes in tea plants using bioinformatics methods, and their structures and promoter sequences. The expression of threePR1, twoPR4and onePR5genes was determined to be induced in stress treatment experiments involving mechanical damage, insect bites, low temperature treatment, and fungal infections. Additionally, the ribonuclease activity of CsPR4 was successfully verified. This is the first study to report the ribonuclease activity of CsPR4 in tea plants. The results can serve as a reference for future studies on PRs in tea plants, offering new insights into information on albinism in tea plants.Keywords:identification;function analysis;pathogenesis-related protein;variegated tea plants
研究领域:
植物生物学
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分子生物学
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基因组学
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植物病理学
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DOI
高影响因子
PLANT DISEASE
·
2019
·Vol. 103
·期 10
·
IF 3.583
Yuchun Wang,Fei Xiong,Qinhua Lu,Xinyuan Hao,Mengxia Zheng,Lu Wang,Nana Li,Changqing Ding,Xinchao Wang,Yajun Yang
Several Pestalotiopsis -like species cause gray blight disease in tea plants, resulting in severe tea production losses. However, systematic and comprehensive research on the diversity, geographical distribution, and pathogenicity of pathogenic species associated with tea plants in China is limited. In this study, 168 Pestalotiopsis -like isolates were obtained from diseased tea plant leaves from 13 primary tea-producing provinces and cities in China. Based on a multilocus (internal transcribed spacer, translation elongation factor 1-α, and β-tubulin gene region) phylogenetic analysis coupled with an assessment of conidial characteristics, 20 Neopestalotiopsis unclassified isolates, seven Pestalotiopsis species, including two novel ( Pestalotiopsis menhaiensis and Pestalotiopsis sichuanensis ), four known ( Pestalotiopsis camelliae , Pestalotiopsis chamaeropis , Pestalotiopsis kenyana , and Pestalotiopsis rhodomyrtus ) and one indistinguishable species, and three Pseudopestalotiopsis species, including two known ( Pseudopestalotiopsis camelliae-sinensis and Pseudopestalotiopsis chinensis ) and one indistinguishable species, were identified. This study is the first to evaluate Pestalotiopsis chamaeropis on tea plants in China. The geographical distribution and pathogenicity tests showed Pseudopestalotiopsis camelliae-sinensis to be the dominant cause of gray blight of tea plants in China. In vitro antifungal assays demonstrated that theobromine not only derepressed mycelial growth of the 29 representative isolates but also increased their growth. Correlation analysis revealed a linear positive relationship between the mycelial growth rate and pathogenicity ( P = 0.0148).
研究领域:
植物病理学
·
真菌学
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产品关联
DOI
PubMed
高影响因子
PLANT FOODS FOR HUMAN NUTRITION
·
2011
·Vol. 66
·期 2
·169-174
·
IF 2.463
Chen Yulong,Jiang Yueming,Shi John,Xu Xinlan,Yang Shaoyu,Duan Jun
The contents of starch and catechins in the fresh leaf of ‘Huang Zhi Xiang’ Oolong tea trees girdled at the bottom, middle (on the big branches) and top (on the small branches) were determined. The study demonstrated that the starch contents from girdled trees were significantly higher ( p < 0.05) than that from non-girdled ones. Furthermore, the contents of (−)−epicatechin (EC), (−)−epigallocatechin (EGC), total catechins (TC) and simple catechins (SC) from girdled trees were significantly higher ( p < 0.05) than those from non-girdled ones. Especially, the contents of (−)−epigallocatechin gallate (EGCG), (−)−epicatechin gallate (ECG) and catechin gallate (CG) from girdled at the middle were also significantly higher ( p < 0.05) than those from the non-girdled. The starch contents were negatively correlated with the contents of (−)−gallocatechin (GC), EC, SC, TC and EGC, while positively correlated with the contents of EGCG and CG in fresh shoots.
研究领域:
植物学
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茶叶科学
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DOI
PubMed
高影响因子
PLANT BIOTECHNOLOGY JOURNAL
·
2025
·
IF 10.5
Mingyue Zhao,Jingming Wang,Xinyuan Hao,Jieyang Jin,Junwei Tang,Yueyue Wang,Mengting Zhang,Tingting Jing,Wilfried Schwab,Ting Gao,Xinchao Wang,Chuankui Song
Summary Cold stress severely limits tea plant ( Camellia sinensis ) productivity, yet the molecular mechanisms underlying cold adaptation remain elusive. Here, we identified a cold-inducible glycosyltransferase, CsUGT71A60, through integrative genome-wide association studies (GWAS) and proteomic profiling. Natural variation in CsUGT71A60 was strongly associated with cold tolerance, as evidenced by linkage disequilibrium analysis of flanking SNPs. Functional characterization revealed that CsUGT71A60 specifically catalyses the glycosylation of cis-zeatin to form cis-zeatin 9-O-glucoside in vitro and in vivo. Overexpression of CsUGT71A60 in Arabidopsis enhanced cold tolerance and agronomic traits, including germination rate, tiller number and seed weight, while delaying flowering. Transient silencing of CsUGT71A60 in tea plants disrupted cis-zeatin homoeostasis, impairing antioxidant defences and osmotic regulation under cold stress. Mechanistically, the transcription factor ARR (TEA021099) directly binds to CRM elements in the CsUGT71A60 promoter, activating its expression to fine-tune cytokinin signalling. This study unveils a dual-function glycosyltransferase that orchestrates stress tolerance and developmental plasticity, offering a strategic target for breeding climate-tolerance crops without yield penalties.
研究领域:
植物学
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遗传学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Lijiao Yu,Lihong Deng,Jieyu Wu,Subhan Mahmood,Ke Yuan,Haoran Wu,Xiaodong Wang,Jing Zhang,Shun Yao
As a key component in tea tree oil (TTO), terpinen-4-ol exhibits ideal physiological activities. However, the high content of terpenes in crude TTO makes it challenging to enrich terpineol-4-ol. In this study, molecular simulations combined with experimental methods were used to select the optimal hydrogen bond acceptor (HBA), tetrabutylammonium chloride (TBAC), which forms a deep eutectic solvent (DES) with terpinen-4-ol in situ. Combined with necessary post-treatment process, it provided a feasible way for purification of TTO. Related DESs were prepared and characterized, and their main properties were also investigated. When the amount of TBAC was three times that of terpinen-4-ol, the formation of hydrogen bonds became more pronounced. At this point, the DES achieved its highest distribution coefficient for terpinen-4-ol (D t = 15.02), as well as superior selectivity for terpinen-4-ol (S t/c = 24.59, S t/γ = 57.69). Water as the back-extractant and n-hexane as the auxiliary extractant facilitated the deep purification of terpinen-4-ol. Response surface analysis and experimental results confirmed that the optimal enrichment conditions were 30 min of enrichment time, an enrichment temperature of 60℃, and 0.5 g/g of n-hexane dosage. The back-enrichment and recovery of TBAC also proved to be satisfactory, and when applied to real tea oil, the developed method achieved over 90 % enrichment efficiency for terpinen-4-ol.
研究领域:
化学工程
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植物化学
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产品关联
DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 7
·1160
·
IF 5.1
Huijuan Zhou,Jinlin Li,Chengwei Yu,Mingming Hu,Bizhen Zhong,Zongcai Tu,Bin Peng
Squid muscle is delicious and nutritious, but it is highly susceptible to spoilage, severely limiting its market development. This study comprehensively evaluated the effects of gelatin (Gel), essential oil (Ess), and Gel + Ess coating on the quality, flavor, and microorganisms of squid muscle during refrigerated storage (4 °C). The results showed that squid muscle treated with Gel + Ess exhibited higher water-holding capacity and sensory evaluation, but lower pH value, chromaticity, and texture parameters than those treated with sterile water (control) during cold storage. The total volatile basic nitrogen (TVB-N) result demonstrated that the shelf life of squid muscle treated with Gel + Ess coating was extended to 12 days at 4 °C, longer than the control group. The Gel + Ess group had the best inhibitory effect on aldehydes and ketones in refrigerated squid muscle, and it could effectively maintain the flavor quality. In addition, the Gel + Ess coating showed an additive inhibition on the growth ofCockerellaandShiwanellacompared to the individual compounds. The Gel + Ess coating is a novel strategy for improving the quality, flavor, and micromolecular metabolites of squid muscle during cold storage.
研究领域:
食品科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Mengli Chen,Zhanyi Xu,Guoxing Chen,Peitong Chen,Chunxia Tian,Jiali Qian,Tiefeng Song,Yongfeng Jin,Guonian Zhu,Ru Yan
Numerous essential oils have been well acknowledged as eco-friendly alternatives to combat insect pests due to synthetic insecticide-induced pest resistance and environment pollution. As a highly commercial essential oil, tea tree oil exhibits excellent insecticidal and repellent activities. However, the molecular mechanism of the olfactory system mediating the tea tree oil-induced repellency against insect pests remains unknown. In our study, mosquito was used as a suitable model to examine the molecular mechanism of tea tree oil-induced repellency against insect pests. The results showed that tea tree oil exhibited excellent spatial and oviposition repellency against Aedes aegypti adults and outstanding repellency against larvae, which were conferred by the main constituent terpinen-4-ol. The reduced repellency in the Orco–/– mutant strain revealed that tea tree oil-induced repellency against mosquitoes was dependent on odorant receptor(s). Moreover, we identified one antenna-biased odorant receptor, AaOr96, that was involved in detecting constituents of tea tree oil to elicit repellency, and the predicted protein–ligand complex indicated that AaOr96 interacted with terpinen-4-ol via van der Waals forces from five key residues. Finally, knocking out AaOr96 resulted in a reduced spatial repellency against A. aegypti by tea tree oil and terpinen-4-ol, and a reduced oviposition repellency by terpinen-4-ol, but not by tea tree oil. Our study not only reveals that tea tree oil has great potential in pest management but also provides more insights into the molecular basis of repellency of essential oils.
研究领域:
昆虫学
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分子生物学
·
害虫管理
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产品关联
DOI
PubMed
高影响因子
PHYTOPATHOLOGY
·
2023
·
IF 3.2
Mr. Jiayu Yin,Ms. Shuang Wu,Ms. Yongli Yang,Dr. Delu Wang,Prof. Yue Ma,Dr. Yongtian Zhao,Prof. Sujitraj Sheth,Miss Honglin Huang,Dr. Baoan Song,Dr. Zhuo Chen
Carvacrol expresses a wide range of biological activities, but the studies of its mechanisms focused on bacteria, mainly involving the destruction of the plasma membrane. In this study, carvacrol exhibited strong antifungal activities against several phytopathogenic fungi and determined a novel antifungal mechanism against Lasiodiplodia theobromae. RNA sequencing indicated that many genes of L. theobromae hyphae were predominately induced by carvacrol, particularly those involved in replication and transcription. Hyperchromic, hypsochromic, and bathochromic effects in the UV-visible absorption spectrum were observed following titration of calf thymus DNA (ctDNA) and carvacrol, which indicated the formation of a DNA–carvacrol complex. Circular dichroism (CD) spectroscopy indicated the response of DNA to carvacrol was similar to 4’, 6-diamidino-2-phenylindole (DAPI), but different from that of ethidium bromide (EB), implying the ionic bonds between carvacrol and ctDNA. Fluorescence spectrum (FS) analysis indicated that carvacrol quenched the fluorescence of double-stranded DNA more than single-stranded DNA, indicating that carvacrol mainly bound to double-stranded DNA. A displacement assay showed that carvacrol reduced the fluorescence intensity of the DNA–DAPI complex through competing with DAPI, but this did not occur for DNA–EB. FS assay revealed that carvacrol bound to AAA sequence on the minor groove of ds-oligonucleotides. The hydroxyl of carvacrol was verified to bind to ctDNA through a comparative test in which structural analogs of carvacrol, including thymol, 4-ethyl-1,2-dimethyl, etc. were analyzed. The current study indicated carvacrol can destruct plasma membranes and bind to the minor groove of DNA, which inhibited the fungal proliferation by disturbing the stability of dsDNA.
研究领域:
植物病理学
·
分子生物学
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DOI
高影响因子
Scientific Reports
·
2015
·Vol. 5
·期 1
·1-15
·
IF 5.578
Jiang Xiaolan,Liu Yajun,Wu Yahui,Tan Huarong,Meng Fei,Wang Yun sheng,Li Mingzhuo,Zhao Lei,Liu Li,Qian Yumei,Gao Liping,Xia Tao
In the present study, proanthocyanidins were qualitatively and quantitatively identified using hydrolysis and thiolysis assays, NP-HPLC, HPLC-ESI-MS, MALDI-TOF-MS, 1 H-NMR and 13 C-NMR techniques in different organs of tea plants. The results showed that in leaves, the tri-hydroxyl, cis - and galloylated flavan-3-ols were the main monomeric catechins units and (epi)catechin was found to be the major unit of polymeric flavan-3-ols when the degree of polymerization was greater than five. In roots, the PAs were found to be abundant and epicatechin formed the predominant extension unit of oligomeric and polymeric PAs. In order to understand the mechanism of proanthocyanidins polymerization, auto-condensation of the flavan-3-ols was investigated. The results showed that the same trimers ( m/z 865) were detected in the extracts of tea plants and in the non-enzymatic in vitro assay, in weak acid as well as weak alkaline solutions at room temperature, when the substrates used were either procyanidin B2 and monomeric flavan-3-ols (epicatechin or catechin), or only procyanidin B2. This suggested that procyanidin B2 not only released carbocation as electrophilic upper units, but also could be used as nucleophilic lower units directly itself, to form the procyanidin trimer in vitro or in vivo .
研究领域:
植物化学
·
代谢组学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2022
·
IF 5.437
Dahe Qiao,Mengsha Tang,Ling Jin,Xiaozeng Mi,Hongrong Chen,Junyan Zhu,Shengrui Liu,Chaoling Wei
Monoterpenes and sesquiterpenes are the most abundant volatiles in tea plants and have dual functions in aroma quality formation and defense responses in tea plants. Terpene synthases (TPS) are the key enzymes for the synthesis of terpenes in plants; however, the functions of most of them in tea plants are still unknown. In this study, six putative terpene biosynthesis gene clusters were identified from the tea plant genome. Then we cloned three new TPS-b subfamily genes, CsTPS08 , CsTPS10 and CsTPS58 . In vitro enzyme assays showed that CsTPS08 and CsTPS58 are two multiple-product terpene synthases, with the former synthesizing linalool as the main product, and β-myrcene, α-phellandrene, α-terpinolene, D-limonene, cis-β-ocimene, trans-β-ocimene and (4E,6Z)-allo-ocimene as minor products are also detected, while the latter catalyzing the formation of α-pinene and D-limonene using GPP as the substrate. No product of CsTPS10 was detected in the prokaryotic expression system , but geraniol production was detected when transiently expressed in tobacco leaves. CsTPS08 and CsTPS10 are two functional members of a monoterpene synthase gene cluster, which were significantly induced during both Ectropis oblique feeding and fresh leaf spreading treatments, suggesting that they have dual functions involved in tea plant pest defense and tea aroma quality regulation. In addition, the differences in their expression levels in different tea plant cultivars provide a possibility for the subsequent screening of tea plant resources with a specific aroma flavor. Our results deepen the understanding of terpenoid synthesis in tea plants.
研究领域:
植物学
·
分子生物学
·
基因组学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 10
·2011
·
IF 5.2
Lilai Mo,Xinzhuan Yao,Hu Tang,Yan Li,Yujie Jiao,Yumei He,Yihe Jiang,Shiyu Tian,Litang Lu
Cuticular wax is a complex mixture of very long-chain fatty acids (VLCFAs) and their derivatives that constitute a natural barrier against biotic and abiotic stresses on the aerial surface of terrestrial plants. In tea plants, leaf cuticular wax also contributes to the unique flavor and quality of tea products. However, the mechanism of wax formation in tea cuticles is still unclear. The cuticular wax content of 108 germplasms (Niaowang species) was investigated in this study. The transcriptome analysis of germplasms with high, medium, and low cuticular wax content revealed that the expression levels ofCsKCS3andCsKCS18were strongly associated with the high content of cuticular wax in leaves. Hence, silencingCsKCS3andCsKCS18using virus-induced gene silencing (VIGS) inhibited the synthesis of cuticular wax and caffeine in tea leaves, indicating that expression of these genes is necessary for the synthesis of cuticular wax in tea leaves. The findings contribute to a better understanding of the molecular mechanism of cuticular wax formation in tea leaves. The study also revealed new candidate target genes for further improving tea quality and flavor and cultivating high-stress-resistant tea germplasms.Keywords:cuticle wax;KCSgene family;tea plant (Camellia sinensis);wax biosynthesis
研究领域:
植物学
·
遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 15
·5889
·
IF 4.6
Yue Zhu,Teng Chen,Tingting Feng,Jiaojiao Zhang,Zejing Meng,Ning Zhang,Gang Luo,Zuhua Wang,Yuxin Pang,Ying Zhou
Nanoemulsion is a new multi-component drug delivery system; the selection of different oil phases can give it special physiological activity, and play the role of “medicine and pharmaceutical excipients all-in-one”. In this paper, we used glycyrrhizic acid as the natural surfactant, andBlumea balsamiferaoil (BB) and tea tree oil (TTO) as the mixed oil phase, to obtain a new green functional composite nanoemulsion. Using the average particle size and polydispersion index (PDI) as the evaluation criteria, the effects of the oil ratio, oil content, glycyrrhizic acid concentration, and ultrasonic time on the nanoemulsion were systematically investigated. The stability and physicochemical properties and biological activities of BB-TTO NEs prepared via the optimum formulation were characterized. The optimal prescription was BB: TTO = 1:1, 5% oil phase, 0.7% glycyrrhizic acid, and 5 min ultrasonication time. The mean particle size, PDI, and zeta potential were 160.01 nm, 0.125, and −50.94 mV, respectively. The nanoemulsion showed non-significant changes in stability after centrifugation, dilution, and 120 days storage. These nanoemulsions were found to exhibit potential antibacterial and anti-inflammatory activities. The minimal inhibitory concentration (MIC) of BB-TTO NEs againstEscherichia coli,Staphylococcus aureus, andPseudomonas aeruginosais 2975 μg/mL, 2975 μg/mL, and 5950 μg/mL, respectively. A lower level of inflammatory cell infiltration and proportion of fibrosis were found in the synovial tissue of AIA rats treated with BB-TTO NEs. These findings demonstrate that the BB-TTO NEs produced in this study have significant potential for usage in antibacterial and anti-inflammatory areas.Keywords:nanoemulsion;Blumea balsamiferaoil;tea tree oil;glycyrrhizic acid;anti-inflammatory;antibacterial
研究领域:
药剂学
·
纳米技术
·
生物科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）