FOOD CHEMISTRY
·
2023
·
IF 8.8
Zuo-Qin Liang,Dong-Dong Song,Zhuo-Cheng Li,Su-Hang Xu,Guo-Liang Dai,Chang-Qing Ye,Xiao-Mei Wang,Xu-Tang Tao
Photoactivatable probes can switch fluorescence on from a weak or nonemission state to improve the sensitivity of the sensing system. In this work, we successfully constructed three highly emissive photoactivatable probes, 2-DP, 1-2-DP and 2-2-DP, for Cu 2+ detection. Under UV irradiation, the photoluminescence quantum yields of 2-DP, 1-2-DP and 2-2-DP display approximately 52.4-, 11.5- and 49.2-fold enhancement, respectively. Cu 2+ selectively quenches the bright photoactivated fluorescence, resulting in an approximately 38-fold fluorescence reduction. The highly selective fluorescence response to Cu 2+ yields an excellent low detection limit of 5.8 nM. Moreover, the photoactivatable probes were successfully applied for Cu 2+ determination in tap water and tea samples with recovery ranges of 95%–105% and 97%–106%, respectively. This work provides a more sensitive and efficient methodology for Cu 2+ detection in heavy metal pollution and food safety.
研究领域:
分析化学
·
环境科学
·
食品安全
✓ 已验证
DOI
PubMed
高影响因子
Food & Function
·
2015
·Vol. 6
·期 6
·2008-2016
·
IF 2.791
Liang Zeng,Jingna Yan,Liyong Luo,Dongying Zhang
Disorders of blood lipid metabolism are the primary risk factors for many diseases. Recently, the effect of Pu-erh tea on blood lipid metabolism has received increasing attention. However, the mechanism underlying its ability to regulate blood lipid metabolism is unclear. We set out to study this through assessing the effects of Pu-erh tea aqueous extract (PTAE) on the central enzymes of blood lipid metabolism, including lipoprotein-associated phospholipase A2 (Lp-PLA2), lecithin: cholesterol acyltransferase (LCAT), 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) and pancreatic lipase (PL). We find that the Lp-PLA2, HMRG and PL activities are inhibited by PTAE in a dose-dependent manner and that the LCAT activity tends to increase with increasing PTAE concentrations. Lineweaver–Burk plot analyses reveal that PTAE acts as a competitive inhibitor for HMGR and PL and as a noncompetitive inhibitor for Lp-PLA2. Moreover, we determine that its active ingredients include catechins, gallic acid, caffeine, free amino acids, and soluble sugar. However, the effect of each ingredient and whether any of them have synergistic effects are still unknown. The results suggest that Pu-erh tea has a potent ability to regulate blood lipid metabolism and knowledge of the mechanisms provides insights into its potential therapeutic application as an alternative hypolipidemic drug.
研究领域:
营养学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Foods
·
2025
·Vol. 14
·期 16
·2759
·
IF 5.1
Mingming Zhang,Zhihui Feng,Fang Wang,Jianxin Chen,Yifan Li,Yuqiong Chen,Junfeng Yin
Appropriate aroma extraction methods are crucial prerequisites for accurately and objectively characterizing the authentic aroma profile of samples. Purified water and ionized water were used as brewing water, and the effects of different tea-to-water ratios, extraction temperatures, and extraction times on the aroma authenticity and component enrichment of tea infusions were compared. The conditions of a tea-to-water ratio of 1 g:10 mL, extraction at 30 °C for 30 or 45 min were identified as the optimal parameter range, which could maximize the enrichment of aroma while maintaining fidelity. The cosine value of the aroma attribute scores between the optimal parameter set and the control group (tea brewed at 1 g:10 mL ratio for 4 min) exceeded 0.979, and the correlation coefficient surpassed 0.828. Test evaluation results indicate the method had good reproducibility and effectively highlighted the differential impacts of ionic content in brewing water on tea aroma constituents. This approach effectively solved the problem of sensory distortion caused by conventional high-temperature and long-duration extraction, enabling precise analysis of how water quality authentically influences tea infusion aroma characteristics.
研究领域:
食品科学
·
分析化学
·
感官科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Wenqing Li,Jingyue Zhang,Linhan Fan,Yun Zhao,Changyan Sun,Wenjun Li,Zhidong Chang
Aniline is an organic pollutant with carcinogenicity and teratogenicity, while F - and Hg 2+ are toxic ions that are easily soluble in water. When they are released to the environment, they will pose a threat to human health. Designing a material that can simultaneously detect three types of pollutants is of great significance. In this paper, a novel rare earth metal organic framework material (Eu-MOF) with three-dimensional structure based on 1-methylimidazole-4,5-dicarboxylic acid was synthesized for the first time through solvent thermal method. It has excellent luminescent performance and can be used as a multifunctional fluorescent probe to detect aniline, F - , and Hg 2+ based on photoinduced electron transfer, energy competitive absorption, and ion exchange mechanisms, with detection limits of 1.79 × 10 -8 , 8.13 × 10 -8 , and 8.83 × 10 -7 M, respectively. It is worth noting that Eu-MOF can detect F - and Hg 2+ in real water samples, such as lake water and green tea water, with favorable recovery rates.
研究领域:
材料科学
·
环境化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Jia Yu,Yuanjie Wu,Chaoxi Zeng,Haoren Wu,Gaofeng Jia,Jianhui Ye,Si Qin,Zhonghua Liu,Meng Shi
Dark tea has long been recognized for its health-promoting benefits, attributed to its complex phytochemical composition. However, the specific bioactive compounds responsible for these beneficial effects remain inadequately characterized. This study aimed to explore the impact of dark tea water extract (DTE) on digestive enzyme activity, antioxidant capacity, and glucose-lipid balance. DTE was fractioned into four fractions using gel separation, followed by analysis with high-performance liquid chromatography and quasi-targeted metabolomics. The 30 % ethanol elution (EEA) and 50 % ethanol elution (EEB) fractions showed stronger antioxidant and enzyme inhibition effects compared to the whole DTE. The EEA fraction was rich in 5 catechins and 26 additional phytochemicals, while the EEB fraction contained high levels of caffeine, ECG, and 29 other phytochemicals. Notably, significant correlations were observed between quercetin-3,4′-O-di-β-glucopyranoside and 3-(2-Naphthyl)-L-alanine with digestive enzyme inhibition. Cellular studies revealed the ability of EEA and EEB to reduce lipid accumulation, improve glycolipid metabolism, and alleviate oxidative stress by increasing SOD, CAT, and GSH levels while decreasing MDA and ROS in HepG2 cells. Furthermore, 34 flavonoids, 2 alkaloids, 2 terpenes, 2 alcohols and polyols, 2 phenylpropanoids and polyketides, 1 organoheterocyclic compound were directly linked to the antioxidant activity and the modulation of glucose and lipid levels. These findings offer valuable insights into the phytochemical profiles of dark tea and its potential health benefits.
研究领域:
食品科学
·
营养学
·
药理学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子