JOURNAL OF FOOD BIOCHEMISTRY
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2025
·Vol. 2025
·期 1
·3869156
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IF 4.2
Tao Chen,Meiquan Yang,Tianmei Yang,Weize Yang,Jinyu Zhang
Amomum tsao-ko (AT) is an important medicinal and edible plant. Its fruit has various colors, commonly available in green, red and dark red colors. There may be significant differences in chemical composition and pharmacological activity among ATs with different fruit colors. In this study, we systematically analyzed the metabolite composition of ATs of different fruit colors and their potential pharmacological mechanisms of action using a combination of widely targeted metabolomics and network pharmacology. Through widely targeted metabolomics techniques, we identified and quantified a variety of metabolites in ATs, including flavonoids, phenolic acids, terpenes, and alkaloids. The results showed that there were significant differences in metabolite composition among AT with different fruit colors, especially in the contents of flavonoids and phenolic acids. Among them, flavonoid components such as kaempferol, isorhamnetin derivatives, and epigallocatechin analogs play a major role in the color formation of ATs. Furthermore, through the network pharmacological analysis, we constructed the metabolite-target-disease network of AT and revealed the potential pharmacological mechanism of AT with different fruit color. Five compounds, ent-Epicatechin, (4e_(6e))-1,ent-Epicatechin(4-hydroxyphenyl)hepta-4,6-dien-3-one, beta-daucosterol_qt, (−)-catechin and quercetin, which are effective in the treatment of tumor, were obtained by screening. They had good docking ability to the protein target of tumor. After screening the metabolites of four kinds of fruit color, quercetin was the common component of four kinds of fruit color AT, but the other four compounds could not be detected. In terms of the relative content of the detected compounds, light red AT > dark red AT > red AT > green AT; generally speaking, the content is relatively high, the more potential biological activity, so in theory, the light red AT is more effective in the treatment of tumor, but further pharmacological verification is needed. This study not only provides a new scientific basis for the chemical differences and pharmacological effects of different fruit colors, but also provides theoretical support.
研究领域:
天然药物化学
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代谢组学
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网络药理学
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药用植物学
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肿瘤药理学
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植物次生代谢
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Miao Liang,Zhimin Zhang,Yajian Wu,Rui Wang,Yuping Liu
A novel headspace solid-phase microextraction method combined with gas chromatography–mass spectrometry was developed to extract volatiles from fresh Amomum tsaoko (FAT) from four districts. The single-factor experiment found an optimum extraction fiber (2 cm) coating with 50/30-μm divinylbenzene-carbon-polydimethylsiloxane, and the response surface design experiment determined that optimal extraction was achieved with equilibration and extraction times of 31 and 32 min at 42°C, respectively. Fifty compounds were identified in FATs from four regions, of which the main volatiles, including ( E )-2-octenal, ( E )-2-decenal, 2-isopropylbenzaldehyde, neral, geranial , and eucalyptol , accounted for a large proportion. Additionally, of the 50 compounds, 32 volatiles determined by orthogonal partial least squares-discriminant analysis contributed to the differentiation of FATs from the four distinct regions. Hierarchical clustering analysis revealed that four FATs were divided into three groups based on the volatiles identified. The four FATs had similar odor profiles, but the odor intensity of the Kongdang sample was weaker than that of the other samples. Therefore, this study not only developed a feasible method to extract volatiles from FAT but also established a theoretical basis for differentiating FATs from different regions.
研究领域:
分析化学
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植物化学
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食品科学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yajian Wu,Runhu Xin,Miao Liang,Wei Guan,Rui Wang,Zhiwei Miao,Yuping Liu
To decode the key odorants in Amomum tsao-ko cobs (ATC) and to examine the impact of extraction methods on key odorants, volatiles of ATC were isolated by both hydrodistillation (HD) and solvent extraction combined with solvent-assisted flavor evaporation (SE-SAFE). In total, 49 aroma-active compounds with flavor dilution (FD) factors between 1 and 8192 were detected by gas chromatography–olfactometry-mass spectrometry, of which 39 and 42 odorants were identified in the HD and SAFE extracts, respectively. The 34 odorants (FD ≥ 16) were further quantitated; odor activity value calculations, aroma reconstitution and omission experiments were conducted to evaluate their contributions to the characteristic odor of ATC, and 11 compounds were confirmed as key odorants of ATC. Furthermore, the majority of the potent odor-active compounds in the two extracts were identical, but their concentrations were significantly different. The outcomes will give a theoretical foundation for developing and utilizing ATC.
研究领域:
食品科学
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分析化学
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香气化学
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产品关联
DOI
高影响因子
Food & Function
·
2021
·Vol. 13
·期 1
·437-450
·
IF 5.396
Xiao-Feng Zhang,Yu-Jun Tang,Xiao-Xian Guan,Xin Lu,Jiao Li,Xiao-Li Chen,Jin-Lan Deng,Jian-Ming Fan
Amomum tsao-ko Crevost et Lemarie (A. tsao-ko) is a well-known dietary spice and traditional Chinese medicine. This study aimed to identify the flavonoids in A. tsao-ko and evaluate their antioxidant and antidiabetic activities in in vitro and in vivo studies. A. tsao-ko methanol extracts possessed a high flavonoid content (1.21 mg QE per g DW) and a total of 29 flavonoids were identified by employing UPLC-MS/MS. In vitro, A. tsao-ko demonstrated antioxidant activity (ORAC value of 34276.57 μM TE/100 g DW, IC50 of ABTS of 3.49 mg mL−1 and FRAP value of 207.42 μM Fe2+ per g DW) and α-amylase and α-glucosidase inhibitory ability with IC50 values of 14.23 and 1.76 mg mL−1, respectively. In vivo, type 2 diabetes mellitus (T2DM) models were induced by a combined high-fat diet (HFD) and streptozotocin (STZ) injection in rats. Treatment with the A. tsao-ko extract (100 mg freeze-dried powder per kg bw) for 6 weeks could significantly improve impaired glucose tolerance, decrease the levels of fasting blood glucose (FBG), insulin, and malondialdehyde (MDA), and increase the superoxide dismutase (SOD) level. Histopathology revealed that the A. tsao-ko extract preserved the architecture and function of the pancreas. In conclusion, the flavonoid composition of A. tsao-ko exhibits excellent antioxidant and antidiabetic activity in vitro and in vivo. A. tsao-ko could be a novel natural material and developed as a related functional food and medicine in T2DM management.
研究领域:
药理学
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营养学
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药物化学
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糖尿病研究
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DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·
IF 3.3
Yulin Zhang,Xusheng Li,Ruijing Liu,Xin Huang,Yiting Yang,Jingquan Yuan,Ying Zhang,Jianxia Sun,Weibin Bai
BACKGROUND Diminished ovarian reserve has a serious impact on female reproduction with an increasing incidence every year. An important cause of this is oxidative stress. Rubi fructus , a traditional medicinal and edible plant, has shown therapeutic effects against gynecological diseases. Vanillic acid, isoquercitrin, kaempferol-3- O -rutinoside, kaempferol-3- O -sophoroside, oleanolic acid, tormentic acid, tiliroside, and ellagic acid are the major bioactive components in R. fructus . However, studies involved in the effectiveness and mechanism of these components in oxidative stress-induced ovarian dysfunction are scarce. RESULTS In this study, the protective mechanisms of the bioactive components were evaluated in human ovarian granulosa cells. Isoquercitrin was significantly superior to other bioactive components in relieving damage in human ovarian granulosa cells induced by 2,2-azobis (2-methylpropionamidine) dihydrochloride, considering enhanced cell viability, reduced reactive oxygen species accumulation, and improved mitochondrial membrane potential level. Isoquercitrin protected human ovarian granulosa cells from oxidative stress by regulating the enzyme activity of glutathione peroxidase, inhibiting cell apoptosis, improving the expression of genes related to oxidative stress, and ameliorating heme oxygenase 1 protein expression. CONCLUSION Isoquercitrin, a bioactive component in R. fructus , has a significant protective effect on oxidative damage induced by 2,2-azobis (2-methylpropionamidine) dihydrochloride in human ovarian granulosa cells, providing evidence for its potential application in protecting ovarian function. © 2024 Society of Chemical Industry.
研究领域:
药理学
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生殖生物学
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氧化应激
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2023
·
IF 4.3
Yajian Wu,Runhu Xin,Miao Liang,Zhimin Zhang,Wei Guan,Rui Wang,Yuping Liu
To characterize the key odorants in Amomum tsao-ko (AT) and to investigate their changes during natural drying, volatile compounds of AT were extracted and analyzed by solvent extraction combined with solvent-assisted flavor evaporation and gas chromatography mass spectrometry–olfactometry. A total of 41 aroma-active compounds with flavor dilution factors ranging from 3 to 6561 were identified in AT. Fourteen odorants were determined to be the key odorants by quantitation analyses, odor activity value (OAV) calculations, recombination and omission experiments. To further investigate the changes in aroma components in AT during natural drying, the concentrations and OAVs of odor-active compounds were measured at 0, 2, 4, 6, 8, 12, 16, and 20 days. The results showed that the number of aroma-active components did not change during natural drying, but their content changed with different trends. The concentration of geraniol showed a continuous upward trend; those of hexanal , octanal , nonanal and pinocarvone decreased first and then increased; those of α -pinene, sabinene , 2-nonanone, ( E )− 2-octenal, 2-nonanol, 4-terpineol, epidolichodial and ( E , E )− 2,4-dodecadienal changed on a fluctuating trend, while those of the other 28 compounds showed a trend of first increasing and then decreasing. These results provide a theoretical basis for further utilization of AT.
研究领域:
食品科学
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农学
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化学工程
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分析化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）