JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2025
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IF 2.8
Zhen Lu,Yingjian Zeng,Zhongkang Zhang,Xiaonan Lu,Guangbin Shang
Sarcandra Glabra is used in the treatment of Immune thrombocytopenia (ITP) clinically. Extractions of Total Flavonoids from Sarcandra Glabra (ETFSG) can improve platelet count in vivo and in vitro. However, the underlying mechanisms of ETFSG on ITP remain unclear. This study aims to investigate the mechanisms of ETFSG in the treatment of ITP via metabolomics and network pharmacology. Specifically, the number of differentiated megakaryocytes and platelet count in ITP model rats can be enhanced by the administration of ETFSG. Untargeted plasma metabolomics analysis revealed that ETFSG regulates the metabolic disorders of amino acids metabolism, arachidonic acid metabolism, pyrimidine metabolism, and citrate cycle metabolism pathways in the ITP animal model. Metabolomics-based network pharmacology analysis suggests that the PI3K-AKT signaling pathway may be involved in ETFSG's promotion of megakaryocyte differentiation. Western blot results indicate that ETFSG increases the expression of P-PI3K and P-AKT. In summary, these findings lay the groundwork for further exploration of the underlying mechanisms.
研究领域:
药理学
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血液学
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系统生物学
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代谢组学
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植物化学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BMC Complementary and Alternative Medicine
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2016
·Vol. 16
·期 1
·1-10
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IF 1.987
Liu Jingjing,Li Xican,Lin Jian,Li Yunrong,Wang Tingting,Jiang Qian,Chen Dongfeng
Background Sarcandra glabra (Caoshanhu) is a traditional Chinese herbal medicine used for treating various oxidative-stressed diseases. The present work evaluated its protective effect on mesenchymal stem cells (MSCs) from oxidative stress and then discussed possible mechanisms underlying this observation. Methods Ethanolic extract of S. glabra (ESG) was investigated by chemical methods for its content of total phenolics, rosmarinic acid, and astilbin. ESG, along with rosmarinic acid and astilbin, was investigated for the effect on the viability of Fenton-treated MSCs using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl (MTT) assay. The observed cell protective effect was further explored by mechanistic chemistry using various antioxidant assays, including DNA protection, •OH-scavenging, •O 2 − -scavenging, FRAP (ferric ion reducing antioxidant power), ABTS + •-scavenging, DPPH•-scavenging, and Fe 2+ -chelating assays. Results Analysis of ESG revealed a content of 46.31 ± 0.56 mg quercetin/g total phenolics, 0.78 ± 0.01 % rosmarinic acid, and 3.37 ± 0.01 % astilbin. Results from the MTT assay revealed that three compounds (rosmarinic acid>astilbin>ESG) could effectively increase the survival of Fenton-treated MSCs. Similarly, in •O 2 − -scavenging, DPPH•-scavenging, and Fe 2+ -chelating assays, rosmarinic acid exhibited more activity than astilbin; while in FRAP, ABTS + •-scavenging assays, astilbin was stronger than rosmarinic acid. Conclusion S. glabra can prevent MSCs from •OH-induced oxidative stress. Such protective effect can be attributed to its antioxidant ability and the presence of two kinds of phytophenols, i.e. caffeoyl derivatives and flavonoids. As the respective representatives of caffeoyl derivatives and flavonoids, rosmarinic acid and astilbin may exert the antioxidant action via direct ROS-scavenging and indirect ROS-scavenging (i.e. Fe 2+ -chelating). The direct ROS-scavenging ability involves hydrogen atom transfer (HAT) and/or electron transfer (ET) pathway. Astilbin engages the latter pathway more, which can be attributed to the larger planar conjugation in A/C fused rings. Rosmarinic acid, on the other hand, shows more HAT and Fe 2+ -chelating potential, which may be due to rosmarinic acid bearing one more catechol moiety whereas astilbin has steric-hindrance from 3-α-L-rhamnose and an H-bonding between 4,5 sites. The antioxidant features of rosmarinic acid can be generalized to other caffeoyl derivatives, while that of astilbin cannot be generalized to other flavonoids because of the difference in chemical structures.
研究领域:
药理学
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细胞生物学
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药物化学
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中医药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）