Auni Aqilah Ahmad Tarmizi,Alina Wagiran,Faezah Mohd Salleh,Lee Suan Chua,Farah Izana Abdullah,Rosnani Hasham,Suraiya Binte Mostafiz
Labisia pumilais a precious herb in Southeast Asia that is traditionally used as a health supplement and has been extensively commercialized due to its claimed therapeutic properties in boosting a healthy female reproductive system. Indigenous people used these plants by boiling the leaves; however, in recent years it has been marketed as powdered or capsuled products. Accordingly, accuracy in determination of the authenticity of these modern herbal products has faced great challenges. Lack of authenticity is a public health risk because incorrectly used herbal species can cause adverse effects. Hence, any measures that may aid product authentication would be beneficial. Given the widespread use ofLabisiaherbal products, the current study focuses on authenticity testing via an integral approach of DNA barcoding and qualitative analysis using HPLC. This study successfully generated DNA reference barcodes (ITS2andrbcL) forL. pumilavar.alataandpumila. The DNA barcode that was generated was then used to identify species ofLabisia pumilain herbal medicinal products, while HPLC was utilized to determine their quality. The findings through the synergistic approach (DNA barcode and HPLC) implemented in this study indicate the importance of both methods in providing the strong evidence required for the identification of true species and to examine the authenticity of such herbal medicinal products.Keywords:Labisia pumila;DNA barcoding;ITS2;rbcL;herbal medicinal products (HMPs);HPLC;authentication
Ultrasonic extraction and nebulization in real-time/carbon fiber ionization mass spectrometry (UEN/CFI-MS) was developed to screen the synthetic drugs adulterated into herbal products such as antidiabetic drug , antihypertensive drug, and hypolipidemic drug . Recently, ambient ionization MS techniques have achieved great advance for rapid analysis of sample surface. However, direct analysis of the analytes inside samples remains a challenge due to a lack of effective online sample extraction procedures . Owing to disappointing desorption efficiency, analytes inside the sample suffer from low detecting sensitivity when applying ambient ionization MS techniques. In this study, online ultrasonic extraction combined with carbon fiber ionization was used for real-time extraction, nebulization and detection of the analytes inside samples. The ultrasonic atomizer could produce a high-frequency vibration to realize online extraction and nebulization of sample. Then, the produced sample droplets could be immediately ionized by the carbon fiber ionization mass spectrometry . UEN/CFI-MS has shown great compatibility to solvents and compounds with a wide range of polarity and has few limitations for the shape of sample. UEN/CFI-MS was successfully applied for the rapid screening of synthetic drugs adulterated into herbal products. Among 37 batches of herbal products, 1 batch of Chinese patent medicine and 6 batches of dietary supplements were detected to be adulterated with the synthetic chemicals without labeling.
危险品化学品经营许可证（带存储）