MICROCHEMICAL JOURNAL
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2024
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IF 4.9
Zihui Jin,Di Sun,Yifan Liu,Bo Xu,Yaqi Jin,Qiyang Zhao,Yue He,Jing Li,Yaohai Zhang,Yongliang Cui
Tangeretin, a polymethoxyflavone abundant in citrus fruits, is acclaimed for its diverse biological benefits to human health and is a significant component of traditional Chinese medicine. This study reports the development of an indirect competitive enzyme-linked immunosorbent assay (icELISA) with monoclonal antibody for quantifying tangeretin in various citrus and herbal medicines. The optimized icELISA demonstrated a half-maximal inhibitory concentration (IC 50 ) of 5.57 ng/mL, encompassing a dynamic range of 1.54–25.38 ng/mL, and showcased average recoveries ranging from 81.65 % to 105.75 % in citrus peel and pulp samples. Extensive analysis of six citrus cultivar samples and four herbal samples was conducted using the developed assay, with results corroborated by Ultra–Performance Liquid Chromatography Tandem Mass Spectrometry (UPLC–MS/MS). These findings confirm the assay’s efficacy and reliability for tangeretin detection, offering a practical method for evaluating this bioactive compound in food.
研究领域:
食品科学
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免疫学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2023
·
IF 2.9
Yuting Xue,Haotian Zhong,Bin Liu,Shuo Qin,Zhengbo Chen,Kai Li,Lirong Zheng,Xia Zuo
Nanozymes have been relatively well explored, and bimetal-doped nanozymes have attracted much exploration due to their superior catalytic activity . We developed bimetallic FeCu/NPCs and Cu/NPCs nanozymes, which have good catalytic properties due to the coordination of Fe and Cu with N and P. The nanozymes acted as sensing elements in a cascade reaction system to effectively recognize seven terpenoids , including menthol (Men), paeoniflorin (Pae), camphor (Cam), paclitaxel (Pac), andrographolide (Andro), ginkgolide A (Gin A), and piperone (Pip). Terpenoids act as inhibitors of acetylcholinesterase (AChE) and reduce the hydrolysis of acetylcholine (ATCh), providing insight into establishing a simple and distinct assay for terpenoids . Notably, the sensor array distinguished seven terpenoids with concentrations as low as 10 ng/mL and achieved high-precision detection of mixed samples with different molar ratios and 21 unknown samples. Finally, the sensor array successfully distinguished and identified multiple terpenoids in herbal samples.
研究领域:
分析化学
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纳米技术
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药学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Di Sun,Yifan Liu,Zihui Jin,Bo Xu,Yaqi Jin,Qiyang Zhao,Yue He,Jing Li,Yaohai Zhang,Yongliang Cui
Limonin is an intensely bitter and highly oxidized tetracyclic triterpenoid secondary metabolite, which is abundant in the Rutaceae and Meliaceae, especially in Citrus. In order to detect limonin content in complex substrates such as citrus and traditional Chinese medicine, monoclonal antibodies specifically recognizing limonin were prepared and an indirect competitive enzyme-linked immunosorbent assay (icELISA) was established. The median inhibition concentration (IC 50 ) was 5.40 ng/mL and the linear range was 1.25–23.84 ng/mL. The average recoveries from citrus peel and pulp samples were 95.9%–118.8% and 77.5%–113.1%, respectively. Moreover, the contents of limonin in 6 citrus samples and 4 herbal samples were analyzed by icELISA and UPLC-MS, and the results of the two methods were consistent. This validation is sufficient to demonstrate that the developed immunoassay is applicable for the detection of limonin in citrus and herbal samples and has the advantage of high efficiency, sensitivity, and convenience.
研究领域:
分析化学
·
免疫学
·
食品科学
·
生药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2019
·
IF 5.399
Yongliang Cui,Jing Zhao,Jie Zhou,Guiyu Tan,Qiyang Zhao,Yaohai Zhang,Baomin Wang,Bining Jiao
Nobiletin, a polymethoxyflavone mainly found in citrus fruits, have been reported to exhibit various beneficial biological activities for human health. It is an important bioactive compound in traditional Chinese medicine, Pericarpium Citri Reticulatae and Fructus Aurantii . To detect the contents of nobiletin in citrus and herb samples, we developed an indirect competitive enzyme-linked immunosorbent assay (icELISA) based on monoclonal antibodies. It possessed a median inhibition concentration (IC 50 ) of 2.43 ± 0.19 ng/mL and a working range of 0.52–12.3 ng/mL. The assay exhibited the average recoveries of 72.5–85.3% in citrus peel, pulp and juice samples. Moreover, eleven citrus cultivars samples and four herb samples were also detected by the icELISA. The nobiletin content varied in different citrus cultivars samples and herb samples, which were confirmed by the ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS). These results indicated that the developed immunoassay was suitable for detecting nobiletin in citrus and herb samples.
研究领域:
分析化学
·
药理学
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食品科学
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中医药
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）