JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2021
·
IF 3.935
Yan-Ting Fan,Ping Zhong,Jin-Di Xu,Shan-Shan Zhou,Ming Kong,Gui-Rong Zhou,Qian Mao,Song-Lin Li
Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown. Herein, a new strategy by integrating multiple-chromatographic approaches to characterize both polysaccharides and small molecules was developed to compare the chemical consistency between MHD and cSHD. Sixteen small molecules were simultaneously qualified and quantified by UPLC-QTOF-MS/MS, the molecular weight distribution of polysaccharides was characterized by HPGPC-ELSD, while the monosaccharide composition and total saccharides content were determined by HPLC-PDA and UV-VIS, respectively. It was found that the molecular weight range and monosaccharide composition of polysaccharides, as well as the composition of small molecules, were identical between MHD and cSHD. However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides. Whether or not these quality variations affect the efficacy and safety of CKF deserves further investigation.
研究领域:
中药学
·
色谱分析
·
药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 7
·期 3
·1036-1045
·
IF 1.938
Xiaozhi Wang,Weijun Zhao,Lingfeng Li,Xiangyang Wang,Peng Li,Yu Wang,Jikui Luo
Sulfites (sulfur dioxide) and inorganic sulfites are types of food additives and preservatives, widely used in the production of food and herbal medicines (HMs). However, the overingestion of sulfites and its associates are harmful to human health and may cause medical complications. Various methods and instruments have been developed for measuring sulfites in foods and HMs, but they typically suffer from many issues, such as high detection limitation, inaccurate and non-reliable results, time and labor-intensive sample preparation, and high cost. Herein, we present a fast, sensitive, and quantitative method to determine sulfites in HMs using field asymmetric-wave ion mobility spectrometry (FAIMS) coupled with a headspace bubbling method. The headspace air bubbling method is effective and efficient at generating stable sulfur dioxide (SO2) in the gas phase for FAIMS analysis. It allows sulfites with a concentration down to 1 mg kg−1 to be easily detected by this new method in 20 min, much shorter than with current technologies. The limits of detection (LOD) and limits of quantification (LOQ) are 1 mg kg−1 and 3 mg kg−1 in HMs, respectively. The new method is of great significance to ensure medical safety and for HM production quality control.
研究领域:
分析化学
·
药学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2024
·
IF 5.6
Min Fan,Tongen Ye,Zitong Wang,Qunchen Li,Chunrui Li,Qingxing Shi,Hualong Qiu,Tao Ma,Xiaoge Nian,Yurong He,Desen Wang
The red imported fire ant (RIFA), Solenopsis invicta Buren, is an invasive pest that is extremely aggressive and thus poses a strong threat to human health, agricultural production, equipment and the environment. In this study, we evaluated the repellent effects of eight Chinese herbal essential oils (EOs) on RIFAs, and identified the main repellent components in the EOs. Results showed that all eight EOs had outstanding repellency to RIFAs, among which A. argyi and S. album EOs had the strongest repellent effects on RIFAs. GC-MS revealed 34 ingredients in A. argyi EO, among which sesquiterpenoids and their oxygenated derivatives accounted for the greatest proportion (50 %), followed by monoterpenes and their oxygenated derivatives (47.06 %) and unknown components (2.94 %). Dipentene, 1-caryophyllene, p-cymene, α-terpineol, and Δ-cadinene were the most abundant compounds. A total of 61 ingredients were identified in S. album EO, including monoterpenes and their oxygenated derivatives (40.98 %), sesquiterpenoids and their oxygenated derivatives (39.34 %), diterpenoids and their oxygenated derivatives (3.28 %), aromatic compounds (3.28 %), and unknown components (13.12 %). Among them, the most abundant compounds were diethyl phthalate, α-cetone, 2-phenylethanol and sandacanol. The GC-EAD results showed that RIFA workers exhibited strong electroantennographic responses to dipentene, α-terpineol and 1-caryophyllene from A. argyi EO, as well as to linalool, 2-phenylethanol and benzyl acetate from S. album EO. Furthermore, all of the above six ingredients had significant repellent effects against RIFAs based on behavioral experiments. In conclusion, A. argyi and S. album EOs have good repellent effects on RIFAs, and the active ingredients include dipentene, α-terpineol, 1-caryophyllene, linalool, 2-phenylethanol and benzyl acetate. Our findings provide new information for the development of green, safe, and efficient repellents against RIFAs.
研究领域:
昆虫学
·
植物化学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Pharmacology
·
2023
·
IF 5.6
Lin Fei,Lin Xinhua,Wang Xuewen,Mei Guanghui,Chen Bing,Yao Hong,Huang Lingyi
Introduction: Selaginella doederleinii Hieron is a traditional Chinese herbal medicine, the ethyl acetate extract from Selaginella doederleinii (SDEA) showed favorable anticancer potentials. However, the effect of SDEA on human cytochrome P450 enzymes (CYP450) remains unclear. To predict the herb-drug interaction (HDI) and lay the groundwork for further clinical trials, the inhibitory effect of SDEA and its four constituents (Amentoflavone, Palmatine, Apigenin, Delicaflavone) on seven CYP450 isoforms were investigated by using the established CYP450 cocktail assay based on LC-MS/MS.Methods: Appropriate substrates for seven tested CYP450 isoforms were selected to establish a reliable cocktail CYP450 assay based on LC-MS/MS. The contents of four constituents (Amentoflavone, Palmatine, Apigenin, Delicaflavone) in SDEA were determined as well. Then, the validated CYP450 cocktail assay was applied to test the inhibitory potential of SDEA and four constituents on CYP450 isoforms.Results: SDEA showed strong inhibitory effect on CYP2C9 and CYP2C8 (IC50 ≈ 1 μg/ml), moderate inhibitory effect against CYP2C19, CYP2E1 and CYP3A (IC50 < 10 μg/ml). Among the four constituents, Amentoflavone had the highest content in the extract (13.65%) and strongest inhibitory effect (IC50 < 5 μM), especially for CYP2C9, CYP2C8 and CYP3A. Amentoflavone also showed time-dependent inhibition on CYP2C19 and CYP2D6. Apigenin and Palmatine both showed concentration-dependent inhibition. Apigenin inhibited CYP1A2, CYP2C8, CYP2C9, CYP2E1 and CYP3A. Palmatine inhibited CYP3A and had a weak inhibitory effect on CYP2E1. As for Delicaflavone, which has the potential to develop as an anti-cancer agent, showed no obvious inhibitory effect on CYP450 enzymes.Conclusion: Amentoflavone may be one of the main reasons for the inhibition of SDEA on CYP450 enzymes, the potential HDI should be considered when SDEA or Amentoflavone were used with other clinical drugs. On the contrast, Delicaflavone is more suitable to develop as a drug for clinical use, considering the low level of CYP450 metabolic inhibition.
研究领域:
药理学
·
中药学
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2023
·
IF 3.4
Jieyu Zhang,Yan Chen,Wenxiang Fan,Linnan Li,Yueming Ma,Zhengtao Wang,Rong Shi,Li Yang
The combined efficacy in lowering serum lipid levels and increasing kidney protection of Plantago asiatica L. seed (Plantago) and Coptis chinensis Franch. rhizoma (Coptis) is far better than the effects of either herb alone. This finding suggests that there must be some degree of herb-herb interactions (HHI) affect potency. Here, we chose geniposidic acid (GPA), acteoside (ACT), and plantagoamidinic acid A (PLA) as active components in Plantago, and berberine (BBR) as the active component in Coptis, and, using transporter gene-transfected Madin-Darby canine kidney (MDCK) cells in combination with specific substrates and inhibitors, investigated Plantago- Coptis HHIs. We also established a UPLC-MS/MS analytical method to determine substrate content. Results showed that PLA in Plantago was a substrate of rOCT1/2 and rMATE1, and had inhibitory effects on rOCT2 and rMATE1. We also found that ACT is a substrate of rMATE1, but GPA was not a substrate of any transporter that we investigated. When BBR was used as the substrate, the inhibition rate of 10 μM PLA was 53.6% on rOCT2 and 31.5% on rMATE1. The inhibition rates of 30 μM ACT and 30 μM GPA on rMATE1 were 47.0% and 31.0%, respectively. Thus, our findings suggest that GPA, ACT, PLA, and BBR have competitive interactions that are driven by the rOCT2 and rMATE1 transporters. These interactions affect the transport and excretion of compounds and result in efficacy changes after co-administration.
研究领域:
药理学
·
药学
·
中药学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2024
·
IF 3.7
Yanting Xu,Jing Zhuang,Shuo Wang,Jianying Dai,Zhi-Long Xiu
The aim of this work was to develop an integrated process of 2,3-butanediol production and extraction of active components from Chinese herbs via fermentation, and evaluate their potential as cosmetic ingredients in vitro. A GRAS strain DL2–2 of Bacillus licheniformis was isolated and the fermentation medium was optimized using the ingredients that could be used in cosmetics. Four types of Chinese herbs were added solely or in combination into the fermentation medium to perform 2,3-butanediol production and active components extraction simultaneously. The release of polysaccharides and flavonoids was promoted, and the antioxidant, whitening and antibacterial abilities were improved. The highest antioxidant and antibacterial activity was obtained from the ferment filtrate of Dendrobium devonianum and Gastrodia elata f. glauca , resulting from the highest contents of flavonoids (0.44 g/L) and polyphenols (0.31 g/L), and 42.2 g/L 2,3-butanediol was also produced. The highest polysaccharide concentration was obtained from the ferment filtrate containing Polygonatum kingianum , which exhibited the highest inhibitory effect on tyrosinase (65 %), similar to that of 1 g/L arbutin. Therefore, the filtrate of 2,3-butanediol-Chinese herbal fermentation broth is promising as an ingredient in the production of natural high-value cosmetics.
研究领域:
生物技术
·
化妆品科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Yulin Zhai,Guoli Li,Kai Peng,Zhaosong Ge,Weibing Zhang,Duxin Li
The resolving power of multiple dimensional liquid chromatography (mD-LC) is multiplicative as it adds dimensions. However, the issue in creating a preparative mD-LC system is that the higher the dimensionality, the more complicated the system configuration. Thus, we presented a new configuration of preparative mD-LC using one set of LC modules and trapping array-based multiple heart-cut interfaces. A preparative two-dimensional liquid chromatography (2D-LC) separation of herbal medicine formulation produced 40 compounds with a purity of >90%. During the separation process, the interface stores the fractions and allocates positions for the fractions from a different dimension; LC draws the fraction from the interface, makes nD separation, and sends isolated fractions to the interface. By repeating this process, we achieved variable dimensionality of LC separations. We also presented a preparative 3D-LC separation of herbal medicines to validate the principle of “less configuration and more dimensionality”. Thus, we can explore the higher dimensional preparative separations. The developed preparative mD-LC displayed exceptional power in the isolation of various compounds and has great potential in the application of natural products.
研究领域:
分析化学
·
天然产物化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2014
·
IF 3.259
Junyan Wang,Minmin Huang,Haihong Hu,Lushan Yu,Su Zeng
The induction of drug-metabolising enzymes is being increasingly recognised for its clinical importance in drug research and development. In the present study, we used a pregnane X receptor (PXR)-mediated reporter gene assay to evaluate the activating effects of various food, herb, and horticultural plant constituents on the PXR and a UGT1A1 reporter gene DNA construct. The results showed that of 102 naturally occurring constituents tested, 50 activated the PXR signaling pathway and induced expression of the UGT1A1 -reporter gene DNA construct. Eleven constituents strongly induced gene expression (a response of >70% compared to that associated with rifampicin), and were further evaluated in in vitro experiments to confirm the induction of UGT1A1 mRNA and protein expression. The results suggest that foods and herbs containing these constituents should be consumed with caution to avoid undesirable drug–drug interactions with co-administered drugs that are substrates for UGT1A1.
研究领域:
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Energy Storage
·
2023
·
IF 9.4
Weiwei Zhu,Dekui Shen,Haijiao Xie
Nitrogen-doped hierarchical porous carbon (N-HPC) was prepared from houttuynia cordata under the combined action of chemical activation and nitrogen-doping. The etching behavior of activator was enhanced by nitrogen doping while the proportion of pyridinic and pyrrolic nitrogen was promoted by the etching behavior. Theoretical calculations reveal that the activator is more likely to adsorb on the nitrogen-doped carbon surface and tend to etch carbon atoms besides the pyridinic, graphitic nitrogen and pyridine-N-oxide. The prepared N-HPC displays a large specific surface area of 2491.5 m 2 g −1 , and the nitrogen atoms in N-HPC exist mainly in the form of pyridinic and pyrrolic nitrogen. The N-HPC electrode exhibits a high specific capacitance of 220.5 F g −1 at 0.5 A g −1 and good rate characteristic in 6 M KOH electrolyte. The assembled symmetric supercapacitors exhibit a high energy density of 9.82 W h kg −1 at a power density of 162.33 W kg −1 . This work reveals the interaction between nitrogen-doping process and etching behavior of the activator, and proposes a novel route for the synthesis of biomass-based carbon material for supercapacitors.
研究领域:
材料科学
·
能源科学
·
植物学
✓ 已验证
产品关联
DOI
高影响因子
Biofabrication
·
2025
·Vol. 17
·期 2
·025006
·
IF 8
Yiran Li,Hongxing Xu,Wenwen Zhao,Li Zhang,Shaohua Wu
The design and development of advanced surgical sutures with appropriate structure and abundant bio-functions are urgently required for the chronic wound closure and treatment. In this study, an integrated technique routine combining modified electrospinning with hot stretching process was proposed and implemented to fabricate poly(L-lactic acid) (PLLA) nanofiber sutures, and the Salvia miltiorrhiza Bunge-Radix Puerariae herbal compound (SRHC) was encapsulated into PLLA nanofibers during the electrospinning process to enrich the biofunction of as-generated sutures. All the PLLA sutures loading without or with SRHC were found to exhibit bead-free and highly-aligned nanofiber structure. The addition of SRHC was found to have no significant influences on the fiber morphology, diameter, and the crystallinity of as-prepared PLLA sutures. Importantly, all the SRHC-contained PLLA nanofiber sutures possessed excellent tensile and knot strength, which were of significant importance for the surgical suture applications. Besides, the antioxidant and anti-inflammatory properties of these sutures obviously enhanced with the increasing of SRHC concentration. Furthermore, the in vitro cell tests illustrated that the high fiber orientation of the sutures was able to efficiently induce the human dermal fibroblasts (HDFs) to migrate in a rapid manner, and the sutures loaded with high content of SRHC could significantly promote the attachment and proliferation of HDFs in comparison. The in vivo diabetic mouse model experiments revealed that all the as-developed PLLA sutures could effectively close the wound, but the PLLA sutures containing high content of SRHC could dramatically promote the wound healing with high quality by shortening the healing time, improving the collagen deposition, neovascularization, and the regeneration of hair follicles, especially compared with commercial polyester (PET) suture. This study offers a simple and easily-handling strategy to develop robust, biodegradable, bioactive, and nanostructured PLLA sutures, which shows huge potential for the treatment of hard-to-heal diabetic wounds.
研究领域:
生物材料学
·
组织工程学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Luwei Fang,Minglei Tian,Xuemin Yan,Wei Xiao,Kyung Ho Row
A convenient, and efficient multi-phase extraction method was used to isolate aristolochic acid from real samples. To increase the efficiency, a new dual ionic liquid-immobilized silica was used as a sorbent and the effects of the adsorption conditions were investigated. IM-BIM@Sil in a methanol/water (60:40, v/v) solution at 60 °C extracted the highest amount of aristolochic acid (16.69 mg/g) compared to the other sorbents examined. The sorbent was then applied to the multi-phase extraction of aristolochic acid. After extraction eight times, 2.4–70.9 × 10 −3 mg/g of aristolochic acid was isolated from nine real samples after several washing and elution steps. The recoveries of aristolochic acid ranged from 70.0% to 110.6% with relative standard deviations of 3.5%–9.1%, highlighting the accuracy of this method.
研究领域:
分析化学
·
药物学
·
植物化学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Xinlin Chen,Shang Xue,Yaolan Lin,Jianguang Luo,Lingyi Kong
In terms of ligand fishing, the amount and the relative activity recovery of enzymes immobilized on magnetic particles and nanoparticles are not preeminent. Therefore, the metal-organic framework (MOF) UiO-66-NH 2 was synthesized to immobilize the porcine pancreatic lipase (PPL) via precipitation-cross-linking, and the resulting novel biological matrices named PPL@MOF manifested high PPL loading capacity (98.31 mg/g) and relative activity recovery (104.4%). Moreover, the novel enzyme-MOF composite was applied to screen lipase inhibitors from Prunella vulgaris L. to enrich and improve the techniques of ligand fishing. As a result, 13 lipase ligands were obtained, and 12 compounds were determined by HPLC-Q-TOF-MS/MS. All of these ligands were further confirmed to be potential inhibitors through the verification of the activity assay and molecular docking . The proposed approach based on PPL@MOF was superior in terms of abundant protein loading capacity, high enzyme catalytic activity and easy preparation process. Taken together, our newly developed method provided a new platform for efficient discovering bioactive molecules from natural herbs.
研究领域:
生物化学
·
药物发现
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）