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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES · 2025 · IF 8.5

Preparation of DCNC chemically cross-linked CMC/PVA composite film for sustainable and strawberry preservation active packaging

Xue Fu,Shuyan Xu,Mingyu Ma

In recent years, with the increasing attention to food safety and environmental protection, the development of biodegradable food packaging materials with comprehensive performance has become a hotspot. This article presents the preparation of a polyvinyl alcohol carboxymethyl cellulose composite film with barrier property. This study utilized formaldehyde nanocellulose as a crosslinking agent to chemically modify composite membranes. Further promote its application in food preservation. The mechanical properties and barrier properties of formaldehyde cellulose (DCNC) polyvinyl alcohol (PVA) carboxymethyl cellulose (CMC) composite films were studied. Apply the prepared DCNC/PVA/CMC composite film to strawberry preservation experiments. The results showed that cross linked modified composite film can more effectively reduce indicators such as weight loss rate, titratable acid content and ascorbic acid content of strawberries, thus effectively prolonging strawberry shelf life by 4 days. This indicates that modified composite films have certain potential application value in the field of food packaging.

CAS / MDL: 5470-11-1
研究领域: 食品科学 · 材料科学
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POSTHARVEST BIOLOGY AND TECHNOLOGY · 2025 · IF 6.8

Biaxially oriented polyethylene (BOPE) and exogenous H2S extend strawberry shelf-life by increasing antioxidant activity and inhibiting pigment accumulation

Ying-Chun Song,Yu-Qi Zhao,Chi-Ming Tang,Pedro Garcia-Caparros,Si-Yu Yang,Jia-Qi Yu,Xiu-Ting Hu,Kang-Di Hu,Zhuo Han,Hua Zhang,Gai-Fang Yao

Hydrogen sulfide (H 2 S) is a gaseous signaling molecule that extends the postharvest storage period of fruit. Biaxially oriented polyethylene (BOPE) films are widely used in fruit packaging due to their excellent properties. By analyzing the phenotypic characteristics of strawberry fruits by comparing with the control, PE and BOPE or in combination with H 2 S at 4 ℃, it was shown that the H 2 S+BOPE treatment reduced decay and water loss, and maintained the best lustre and firmness than other groups. A total of 7,258 co-differentially expressed genes (DEGs) were identified by comparing H 2 S, H 2 S+PE and H 2 S+BOPE with Control by transcriptome analysis. GO, WGCNA and KEGG analyses showed that DEGs were mainly enriched in starch and sucrose metabolism, pigment metabolism and antioxidant activity from the MEyellow and MEblack modules. Moreover, these candidate genes were screened, and the expression level of CHS , PAL and DFR were significantly decreased in H 2 S+BOPE group than other groups by RT-qPCR. Physiological assessments indicated that H 2 S+BOPE treatment significantly delayed chlorophyll degradation, anthocyanin accumulation and TA content, increased reducing sugar content, carotenoid and POD activity, and maintained low MDA content in strawberries. PCA and heatmap showed that POD and pigments were main factors and the correlation analysis proved that POD activity exhibited a positive correlation with the expression of DFR (0.57) and bHLH3 (0.56), carotenoid content (0.54), and it showed a negative correlation with chlorophyll contents (-0.37). These results indicated that BOPE could effectively improve the appearance and antioxidant activity of strawberry fruits, thereby prolonging their postharvest storage period.

CAS / MDL: 207683-19-0
研究领域: 植物学 · 食品科学
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Horticulturae · 2025 ·Vol. 11 ·期 12 ·1417 · IF 3

Comparative LC-MS/MS Metabolomics of Wild and Cultivated Strawberries Reveals Enhanced Triterpenoid Accumulation and Superior Free Radical Scavenging Activity in Fragaria nilgerrensis

Mingzheng Duan,Liuyuan Bao,Ting Jiang,Kangjian Song,Yubo Chen,Sijiu He,Xiande Duan,Muhammad Ikram,Shunqiang Yang,Muhammad Junaid Rao

Strawberry fruit quality is linked to its phytochemical composition, yet the diversity of non-volatile terpenoids remains largely unexplored. Therefore, a comparative LC-MS/MS-based metabolomic analysis of terpenoid profiles was conducted using three commercial cultivars (Fragaria×ananassa) and a wild accession ofFragaria nilgerrensis(HM). Results from this study showed that the HM cultivar had a total terpenoid abundance 5–6 times higher than the commercial cultivars. The HM cultivar was uniquely enriched in specific triterpenoids, such as 3β,6β,19α,24-Tetrahydroxyurs-12-en-28-oic acid and 13,27-Cyclo-2,3-Dihydroxy-11,19(29)-Ursadien-28-Oic Acid, which was over 450 times higher than the ‘Danxue’ and ‘Fenyu’ commercial strawberry cultivars. Conversely, sesquiterpenoids like Alismol and Pterocarpol were 100 times lower in HM than in the commercial cultivars. This enhanced triterpenoid accumulation strongly correlated with a superior capacity to scavenge free radicals in vitro, with HM showing maximum capacity, as measured by the DPPH assay. These findings highlight the value of wild strawberry germplasm as a reservoir of biochemical diversity for breeding strawberries with enhanced functional quality.

CAS / MDL: 1898-66-4
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ACTA PHYSIOLOGIAE PLANTARUM · 2014 ·Vol. 36 ·期 9 ·2441-2451 · IF 1.524

Detachment-accelerated ripening and senescence of strawberry (Fragaria × ananassa Duch. cv. Akihime) fruit and the regulation role of multiple phytohormones

Chen Jingxin,Mao Linchun,Mi Hongbo,Zhao Yuying,Ying Tiejin,Luo Zisheng

To study the detachment stress on the ripeness of strawberry fruit, physiological characteristics of strawberry fruit on and off plant during ripeness and senescence processes were investigated. The results indicated that the ripeness of strawberry fruit upon detachment was accelerated, in terms of firmness, soluble solid content and especially color development. The color of fruit off plant changed rapidly from white to full red in 1–2 days. The respiratory rate in fruit off plant was strengthened, higher than that on plant. Abscisic acid level and ethylene production in fruit off plant were also higher than those on plant and auxin degradation was exacerbated by detachment. Expression levels of FaMYB1 , FabHLH3 and FaTTG1 were generally reduced with phenotypes of redder color and more anthocyanin accumulation in fruit off plant. Results also suggested that the detachment initially stimulated ethylene and abscisic acid production and auxin degradation, which modulated ripening-related gene expression and at last enhanced fruit pigmentation.

CAS / MDL: 128-37-0
研究领域: 植物学 · 生理学
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES · 2021 ·Vol. 23 ·期 1 ·101 · IF 5.924

FvMYB79 Positively Regulates Strawberry Fruit Softening via Transcriptional Activation of FvPME38

Jianfa Cai,Xuelian Mo,Chenjin Wen,Zhen Gao,Xu Chen,Cheng Xue

Strawberry is a soft fruit with short postharvest life, due to a rapid loss of firmness. Pectin methylesterase (PME)-mediated cell wall remodeling is important to determine fruit firmness and softening. Previously, we have verified the essential role ofFvPME38in regulation of PME-mediated strawberry fruit softening. However, the regulatory network involved in PME-mediated fruit softening is still largely unknown. Here, we identified an R2R3-type MYB transcription factor FvMYB79, which activates the expression level ofFvPME38, thereby accelerating fruit softening. During fruit development,FvMYB79co-expressed withFvPME38, and this co-expression pattern was opposite to the change of fruit firmness in the fruit of ‘Ruegen’ which significantly decreased during fruit developmental stages and suddenly became very low after the color turning stage. Via transient transformation, FvMYB79 could significantly increase the transcriptional level ofFvPME38, leading to a decrease of firmness and acceleration of fruit ripening. In addition, silencing ofFvMYB79showed an insensitivity to ABA-induced fruit ripening, suggesting a possible involvement ofFvMYB79in the ABA-dependent fruit softening process. Our findings suggest FvMYB79 acts as a novel regulator during strawberry ripening via transcriptional activation ofFvPME38, which provides a novel mechanism for improvement of strawberry fruit firmness.Keywords:fruit softening;strawberry;FvMYB79;FvPME38

研究领域: 植物生物学 · 分子遗传学 · 农学
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LWT-FOOD SCIENCE AND TECHNOLOGY · 2023 · IF 6

Characterization of chitosan/eugenol-loaded IRMOF-3 nanoparticles composite films with sustained antibacterial activity and their application in postharvest preservation of strawberries

Kejun Chen,Charles Brennan,Jianxin Cao,Guiguang Cheng,Lin Li,Yuyue Qin,Haiyan Chen

In this work, we prepared chitosan/eugenol-loaded IRMOF-3 nanoparticles (CS/IRMOF-3-EU) composite films using (0, 2, 4, and 6 wt%) isoreticular metal-organic framework (IRMOF-3) nanoparticles loaded with eugenol (IRMOF-3-EU), co-blended with chitosan to develop environmentally friendly food packaging materials with sustained antimicrobial activity. The microstructure and physicochemical properties of the composite films were investigated. The addition of IRMOF-3-EU to the CS matrix significantly enhanced the UV-blocking property and elongation at the break of the composite films; the CS/IRMOF-3-EU composite films inherited the antioxidant and antibacterial properties of eugenol (EU), and the DPPH radical scavenging ability was increased to 53.8% with the addition of 6% IRMOF-3-EU, while it showed excellent antibacterial ability; in addition, the CS/IRMOF-3-EU composite films achieved the sustainable release of eugenol to achieve the long-lasting antibacterial effect. Experiments on strawberry preservation applications showed that the CS/IRMOF-3-EU films slowed down the deterioration of strawberry quality, delayed the decline of hardness, effectively suppressed weight loss, H 2 O 2 content, and malondialdehyde content, and delayed the decline of catalase activity and peroxidase activity. It showed that the CS/IRMOF-3-EU composite films could be used to maintain the postharvest quality and prolong the shelf life of strawberries.

CAS / MDL: 97-53-0
研究领域: 食品科学 · 材料科学 · 植物病理学
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JOURNAL OF EXPERIMENTAL BOTANY · 2018 ·Vol. 69 ·期 10 ·2595-2608 · IF 5.354

Reduced Anthocyanins in Petioles codes for a GST anthocyanin transporter that is essential for the foliage and fruit coloration in strawberry

Luo Huifeng,Dai Cheng,Li Yongping,Feng Jia,Liu Zhongchi,Kang Chunying

The red color of the foliage and fruit in strawberry comes from anthocyanins stored in the vacuole; however, how this anthocyanin accumulation is regulated remains unclear. A reduced anthocyanin in petioles (rap) mutant was identified in an N-ethyl-N-nitrosourea (ENU) mutagenized population of YW5AF7, a white-fruited variety of the wild strawberry Fragaria vesca. The causative mutation was identified to be a premature stop codon in a glutathione S-transferase (GST) gene. In addition to the foliage coloration, RAP also mediates fruit pigmentation and acts downstream of the fruit-specific transcription factor FvMYB10. Among all eight GST genes in the same subfamily, RAP is most abundantly expressed in the ripening fruit. Expression analysis and transient expression assays demonstrated that RAP is the principal transporter of anthocyanins among the paralogs. Moreover, domain-swap experiments showed that both the N- and C-terminals of RAP are essential for the binding capability of anthocyanins. In addition, transient knock-down of RAP resulted in reduced fruit coloration in cultivated strawberry. Collectively, our results demonstrate that RAP encodes the principal GST transporter of anthocyanins in the strawberry foliage and fruit, and it could be modified to alter the fruit color in strawberry.

研究领域: 植物学 · 遗传学 · 分子生物学
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FOOD CHEMISTRY · 2024 · IF 8.5

Preparation and characterization of quercetin@ZIF-L/GO@AgNPs nanocomposite film for room-temperature strawberry preservation

Chen Yin,Xin Ding,Zhihao Lin,Jiayong Cao,Weiye Shi,Jian Wang,Dan Xu,Donghui Xu,Yuan Liu,Guangyang Liu

Fresh strawberries are easily contaminated by microorganisms after picking. Therefore, how to effectively store and keep fresh strawberries has been a hot topic for scientists to study. In this study, we prepared a leaf shaped metal organic framework nanomaterial loaded with quercetin (Quercetin@ZIF-L) at first, which can achieve effective loading of quercetin (96%) within 45 min and has a controlled release effect under acidic conditions. In addition, by cleverly combining satellite graphene oxide @ silver nanoparticles (GO@AgNPs) with slow precipitation performance, Quercetin@ZIF-L/GO@AgNPs nanocomposite film with larger pore size and larger specific surface area was prepared by scraping method. The characterization data of water flux , retention rate, flux recovery rate and water vapor permeability show that the composite film has good physical properties. The experiment of film packaging showed that the fresh life of strawberry could be extended from 3 to 8 days, which significantly improved the storage and freshness cycle of strawberry. At the same time, the metal migration test proved that the residual amount of silver ion in strawberry met the EU standard and zinc ions are beneficial to the health, enriching the types of high-performance fresh-keeping materials and broadening the application.

CAS / MDL: 693-98-1 25322-68-3
研究领域: 食品科学 · 材料科学
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JOURNAL OF FOOD BIOCHEMISTRY · 2018 ·Vol. 42 ·期 3 ·e12527 · IF 1.552

Quality and postharvest-shelf life of cold-stored strawberry fruit as affected by gum arabic (Acacia senegal) edible coating

Haroon Elrasheid Tahir,Zou Xiaobo,Shi Jiyong,Gustav Komla Mahunu,Xiaodong Zhai,Abdalbasit Adam Mariod

The impact of gum arabic (GA) (10 and 15% w/v) on the quality and postharvest-shelf life of strawberry at storage condition of 4 ± 1°C for 10 days were evaluated. Significant ( p < .05) differences were found for the overall 15% GA-coating as compared with the control. The application of GA-coatings maintained the total contents of phenolic, anthocyanin and total soluble solid (TSS) but significantly ( p < .05) retarded the increase in polyphenol oxidase (PPO) activity, reduced weight loss, and completely inhibited fungal infections. Moreover, the 15% GA edible coating retained the color, improved the firmness, and increased antioxidant activity compared with control. Likewise, sensory analysis results also showed the effectiveness of 15% GA-coating by retaining the quality of strawberry fruits. The results show that 15% of GA-coating can be an effective strategy for enhancing the quality of strawberry fruits during cold storage without any negative effect. Practical applications The edible coating has demonstrated to be a promising food preservation method and is progressively used for the commercial processing of many fruits and vegetables. However, gum arabic has not been used for preserving the quality of highly perishable fruit such as strawberries. The result suggests that gum arabic coatings can be a valuable biochemical means of preserving strawberries during refrigerated storage and could be used as an alternative to the synthetic formulas of fruit and vegetable packaging. Thus, gum arabic coating could provide horticultural and food processors a useful way to improve the quality, prolong shelf life, and marketing strategy.

CAS / MDL: 25249-54-1 9003-39-8
研究领域: 食品科学 · 植物生理学
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PLANTA · 2015 ·Vol. 243 ·期 1 ·183-197 · IF 3.263

Transcriptome profiling of postharvest strawberry fruit in response to exogenous auxin and abscisic acid

Chen Jingxin,Mao Linchun,Lu Wenjing,Ying Tiejin,Luo Zisheng

Main conclusion Auxin and abscisic acid regulate strawberry fruit ripening and senescence through cross-talk of their signal transduction pathways that further modulate the structural genes related to physico-chemical properties of fruit. The physiological and transcriptomic changes in harvested strawberry fruits in responses to IAA, ABA and their combination were analyzed. Exogenous IAA delayed the ripening process of strawberries after harvest while ABA promoted the postharvest ripening. However, treatment with a combination of IAA and ABA did not slow down nor accelerate the postharvest ripening in the strawberry fruits. At the molecular level, exogenous IAA up regulated the expressions of genes related to IAA signaling, including AUX/IAA , ARF , TOPLESS and genes encoding E3 ubiquitin protein ligase and annexin, and down regulated genes related to pectin depolymerization, cell wall degradation, sucrose and anthocyanin biosyntheses. In contrast, exogenous ABA induced genes related to fruit softening, and genes involved in signaling pathways including SKP1 , HSPs , CK2 , and SRG1 . Comparison of transcriptomes in responses to individual treatments with IAA or ABA or the combination revealed that there were cooperative and antagonistic actions between IAA and ABA in fruit. However, 17 % of the differentially expressed unigenes in response to the combination of IAA and ABA were unique and were not found in those unigenes responding to either IAA or ABA alone. The analyses also found that receptor-like kinases and ubiquitin ligases responded to both IAA and ABA, which seemed to play a pivotal role in both hormones’ signaling pathways and thus might be the cross-talk points of both hormones.

CAS / MDL: 128-37-0
研究领域: 植物学 · 分子生物学
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LWT-FOOD SCIENCE AND TECHNOLOGY · 2020 · IF 4.006

Improvement of storage quality of strawberries by pullulan coatings incorporated with cinnamon essential oil nanoemulsion

Yifu Chu,ChengCheng Gao,Xiaoya Liu,Ni Zhang,Tian Xu,Xiao Feng,Yuling Yang,Xinchun Shen,Xiaozhi Tang

This study was aimed at evaluating the effect of pullulan coating incorporated with cinnamon essential oil (CEO) nanoemulsion on the shelf life and senescence of fresh strawberries during room storage. Pullulan-CEO nanoemulsion prepared using ultrasound treatment had smaller particle size (162.1 nm) and more uniform distribution of oil droplets. After six days of storage, pullulan-CEO nanoemulsion coating remarkably lowered the loss in fruit mass, firmness, total soluble solids and titratable acidity of strawberries. In addition, pullulan-based nanoemulsion coated strawberries showed the strongest antimicrobial activity against bacteria and molds (2.544 and 1.958 log CFU/g, respectively) compared to control and pure pullulan coating groups. It is mainly due to the antimicrobial activities of CEO dispersed in nano size in pullulan matrix. This study revealed that pullulan-CEO nanoemulsion coating showed great potential to prolong the shelf life of fresh strawberries during room storage.

CAS / MDL: 7385-67-3
研究领域: 食品科学 · 植物病理学
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JOURNAL OF FOOD PROTECTION · 2022 · IF 2.745

Stereoselective behavior and residues of the imazalil during strawberry growth and strawberry wine production

Jia Zhang,Wei Jiang,Zhihang Jia,Wenjie Zhang,Ting Zhang,Meng Wei

Imazalil is a chiral fungicide widely used to protect strawberries against gray mold, which may pose threats to food safety. This study aims to investigate the stereoselective behavior of imazalil during strawberry growth and strawberry wine production. A method was proposed and validated for the extraction and quantitative analysis of imazalil residues in strawberry, strawberry pomace, and strawberry wine by using ultra-high performance liquid chromatography-tandem mass spectrometry. The method exhibited mean recoveries ranging from 86.2% to 119.7% with relative standard deviations of 0.1–11.3%. The dissipation curve of imazalil during strawberry growth followed the first-order kinetic model with a half-life ranging from 6.5 to 7.1 days. Significant enantioselectivity of imazalil was observed in strawberry grown under field conditions and strawberry wine production process, with enantiomeric fraction values ranging from 0.51 (2 h) to 0.42 (27d) and from 0.48 (0d) to 0.52 (10d), respectively. (+)-imazalil was preferentially degraded in strawberry under field conditions, while (−)-imazalil was preferentially degraded during the fermentation process. The processing factor was lower than 1 for each procedure, indicating that the wine-making process can reduce imazalil residue in strawberry. These findings may facilitate a more accurate risk assessment of imazalil and provide important guidance for the safe and efficacious use of imazalil in agriculture.

CAS / MDL: 35554-44-0
研究领域: 食品科学 · 农业化学
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