JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
·
2025
·
IF 6.2
Guodong Hong,Zhenqiang Yu,Dexin Kong,Taoli Huhe,Rui Shan,Haoran Yuan,Yong Chen
Currently, the selection of iron valence states in composite-modified magnetic biochar prepared by co-pyrolysis exhibits a high degree of blindness. Therefore, this study compares the performance of biochar preparation via co-pyrolysis of three different iron valences (Fe 2 + , Fe 3+ , and Fe 6+ ) and potassium oxalate (K 2 C 2 O 4 ) composite modification for tetracycline (TC) removal. The results indicate that the co-activation of potassium oxalate with different iron valences led to the formation of magnetic biochar with varying elemental contents, morphologies, and structures. Batch adsorption experiments demonstrate that KF3-BC has a high tetracycline removal rate over a wide pH range (3 – 11) and at high coexisting ion concentrations (1 – 100 mmol/L). Furthermore, experiments with synthetic swine wastewater in different matrices, the adsorption capacity of KF3-BC for TC (at a concentration of 100 mg/L) exceeds 81.09 mg/g. Additionally, under optimal conditions of 30 °C and pH = 5, the theoretical maximum adsorption capacity (Q max ) for TC is as follows: KF3-BC (236.91 mg/g) > KF6-BC (169.42 mg/g) > KF2-BC (51.34 mg/g) > BC (38.41 mg/g). The adsorption processes of KF2-BC, KF3-BC, and KF6-BC all conform to the Pseudo-second-order and Freundlich models, implying that TC adsorption occurs at the multilayer molecular adsorption on heterogeneous surfaces, primarily driven by chemisorption. The primary adsorption mechanism between KF-BCs and TC molecules is the π–π EDA interactions. Additionally, hydrogen bonding, cation–π complexation, electrostatic interactions, and pore filling also contribute to the adsorption process. This study can assist in selecting the optimal iron source and chemical activator for composite modification of biochar for the adsorption of TC in wastewater.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 6
·840
·
IF 4.7
Jianghui Yao,Enhe Bai,Yanwen Duan,Yong Huang
The purpose of this study was to evaluate the efficacy of ethanol extracts fromTorreya grandisseed (EST) as a functional food in hyperuricemia mice. We investigated EST by analyzing its chemical composition. Using a mouse model of hyperuricemia induced by potassium oxonate (PO), we evaluated the effects of EST on uric acid (UA) production, inflammation-related cytokines, and gut microbiota diversity. The primary constituents of EST consist of various flavonoids and phenolic compounds known for their antioxidant and anti-inflammatory properties in vitro. Notably, our findings demonstrate that EST significantly reduced UA levels in hyperuricemia mice by 71.9%, which is comparable to the effects observed with xanthine treatment. Moreover, EST exhibited an inhibitory effect on xanthine oxidase activity in mouse liver, with an IC50value of 20.90 μg/mL (36%). EST also provided protective effects to the mouse kidneys by modulating oxidative stress and inflammation in damaged tissues, while also enhancing UA excretion. Finally, EST influenced the composition of the intestinal microbiota, increasing the relative abundance of beneficial bacteria such asAkkermansia muciniphila,Corynebacterium parvum,Enterorhabdus, Muribaculaceae,Marvinbryantia, andBlautia. In summary, our research unveils additional functions ofTorreya grandisand offers new insights into the future of managing hyperuricemia.
研究领域:
药理学
·
营养学
·
生物化学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2018
·
IF 3.47
Rui-dan Wang,Guan-hua Su,Lin Wang,Qing Xia,Rui Liu,Qun Lu,Jiu-liang Zhang
This study was aimed at evaluating the hypouricemic effect of rape bee pollen from Qinghai province in China. Rape bee pollen crude extracts (RPCE) was obtained by n-butanol extraction and purified by polyamide resin and AB-8 resin respectively to generate five fractions (fraction 1–5). In vitro , fraction 5 had strongest inhibition on xanthine oxidase (XO) (IC 50 = 0.21 ± 0.02 mg mL −1 ) among five fractions and had reversible and mixed inhibitory effect on XO. Fraction 5 was further purified and then generated fraction 5′. In vivo , fraction 5′ had lower serum UA level and could reduce serum BUN, Cr levels and hepatic XO activity more effectively than RPCE in potassium oxonate-induced hyperuricemic mice . Moreover, fraction 5′ had higher CAT activity and content of GSH in hyperuricemic mice. Six compounds including quercetin-3-O-(2″-O-glucopyranosyl)-glucopyranoside (peak 1), quercetin-3-O-(2″-O-glucopyranosyl)-rhamnopyranoside (peak 2), kaempferol-3-O-(2″-O-glucopyranosyl)-glucopyranoside (peak 3) and N′, N″-di- p -coumaroylspermidine (peak 4–6) in RPCE were identified by using HPLC-ESI-QTOF-MS/MS, whereas only peak 4 to 6 were found in fraction 5′. This study shows that rape bee pollen has potential clinical application value as an effective anti-hyperuricemia bioactive drug or functional food.
研究领域:
药理学
·
生物化学
·
食品科学
·
医学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF PHARMACY AND PHARMACOLOGY
·
2020
·Vol. 72
·期 4
·551-560
·
IF 2.571
Xu Wen-hao,Wang Han-tao,Sun Ying,Xue Zhen-cheng,Liang Ming-li,Su Wei-ke
ObjectivesTo investigate the antihyperuricemia and nephroprotective effects of Orthosiphon stamineus extracts on hyperuricemia (HUA) mice and explore the potential mechanisms.MethodsOrthosiphon stamineus extracts were extracted using 50% ethanol and enriched using ethyl acetate, and characterised utilising UPLC/ESI-MS. A potassium oxonate (PO) induced hyperuricemic mouse model was used to evaluate antihyperuricemia and nephroprotective effects of O. stamineus ethyl acetate extracts (OSE).Key findingsEight constituents from OSE were identified and OSE treatment ameliorated HUA by regulating key indicators of kidney dysfunction and xanthine oxidase, adenosine deaminase activity and urate transporters in hyperuricemic mice. Moreover, in renal histopathology analysis, OSE significantly alleviated kidney injury.ConclusionsThese findings demonstrate that OSE has antihyperuricemic and nephroprotective effects on PO-induced HUA mice and those results indicate that OSE could be a safe and effective agent or functional ingredient for treating HUA.
研究领域:
药理学
·
肾病学
·
植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Li Hao,Yulian Ding,Yan Fan,Chensi Xia,Yuqian Meng,Qiannan Jia,Jian Zhang,Changhu Xue,Hu Hou
Hyperuricemia (HUA) endangers renal function and induces gout. Although peptides from Antarctic Krill (AKP) showed anti -hyperuricemic effects, the underlying mechanism remained unclear. The study investigated the anti -hyperuricemic effect via xanthine oxidase (XOD) inhibitory activity, an adenosine-induced HK-2 cell model, and a potassium oxonate (PO)-induced mouse model. The AKP prepared using alkaline protease exhibited a strong XOD inhibitory activity with a low IC 50 value of 3.232 mg/mL. AKP (5 mg/mL) reduced the uric acid (UA) content by 40.50% in HK-2 cells ( p < 0.01). In addition, AKP (600 mg/kg/d) reduced serum UA level by 54.37% in a mouse model ( p < 0.01) and had an alleviating effect on HUA-induced inflammation. Besides, AKP supplementing could regulate the mRNA levels of renal UA urate transporters (GLUT9, ABCG2, OAT1, OAT3, and NPT1) to promote UA excretion. According to non-targeted metabolomics, AKP regulated metabolic disorders in HUA mice by proximal tubule bicarbonate reclamation, taurine and hypotaurine metabolism, and butanate metabolism, etc, providing useful clues for research on the molecular mechanism of AKP. In addition, AKP alleviated gout by reducing paw swelling and inflammatory factors (IL-6, TNF-α, IL-1β) levels.
研究领域:
药理学
·
生物化学
·
代谢组学
·
分子生物学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2022
·
IF 10.753
Tao Wang,Lu Xue,Yonghong Liu,Lu Zhang,Baoshan Xing
In this study, we developed a simple strategy to synthesize a N self-doped hierarchically porous carbon adsorbent (LPC-NC) derived from biomass using potassium oxalate monohydrate and calcium carbonate and remove tetracyclines that are major antibiotics frequently measured in surface water. In the pyrolysis process, the N-enriching lotus seed pots biomass decomposed and formed a porous carbon matrix with self-doped N. The LPC-NC displayed high adsorption amount (506.6 mg/g for tetracycline (TTC) and 445.3 mg/g for oxytetracycline (OTC)), short equilibrium time (30 min) and stable reusability (the decline efficiency<8.0% after five cycles). Batch adsorption experimental and theoretical studies showed that the high adsorption capacity of LPC-NC for tetracyclines was mainly ascribed to the self-doped pyridinic-N species and the adsorption capacity of pyridinic-N species at the edge location was better than that of pyridinic-N species at the vacancy location. Importantly, we believe that the high adsorption performance of LPC-NC for tetracyclines is due to the activation of carbon π electrons by destroying the integrity of conjugation on LPC-NC, thus enhancing the π-π interaction between LPC-NC and tetracyclines. In addition, the results of solid-state nuclear magnetic resonance (NMR) confirmed that the hierarchically porous structure of LPC-NC was conducive to the adsorption of tetracyclines. These insights provide new ideas for the rational design of N-doped carbon-based adsorbents for the efficient removal of tetracyclines.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Bioengineered
·
2021
·
IF 3.269
Xia Zhao,Jing Wang,Liying Tang,Pei Li,Jing Ru,Yuzhi Bai
The study was designed to explore the effects of Withaferin A (WFA) on hyperuricemia-induced kidney injury and its action mechanism. Potassium oxonate (PO) was employed to establish the hyperuricemic mouse model. The pathological changes of renal tissue were evaluated by hematoxylin-eosin and masson trichrome staining. The levels of creatinine, blood urea nitrogen (BUN), uric acid (UA) and xanthine oxidase (XOD) were detected using corresponding commercial kits. Expressions of collagen-related and apoptosis-associated proteins in renal tissues were, respectively, evaluated by immunofluorescence and western blotting. Cell apoptosis was detected by TUNEL assay, and transporter expressions using western blotting. Followed by WFA, NRK-52E cells were treated with UA before evaluation of apoptosis and fibrosis. Results indicated that WFA ameliorated renal damage, improved kidney function, and decreased levels of creatinine, BUN, UA, and XOD in PO-induced hyperuricemic mice. Furthermore, WFA significantly prevented renal fibrosis and increased the expression of collagen-related proteins. Similarly, WFA markedly inhibited renal apoptosis, accompanied by changes of apoptosis-related proteins. Importantly, expression of transporters responsible for the secretion of organic anion transporter 1 (OAT1), OAT3, ATP-binding cassette subfamily G member 2 (ABCG2) was remarkably enhanced whereas that of urate transporter 1 (URAT1) and glucose transporter 9 (GLUT9) was reduced in renal tissues of mice with hyperuricemia. In vitro study revealed that WFA notably ameliorated UA-induced cell fibrosis and apoptosis. Taken together, WFA improves kidney function by decreasing UA via regulation of XOD and transporter genes in renal tubular cells.
研究领域:
药理学
·
肾病学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Chao Li,Kai Sun,Yifan Sun,Yuewen Shao,Guoming Gao,Lijun Zhang,Shu Zhang,Xun Hu
Pre-carbonization is one frequently used pre-treatment for production of activated carbon (AC) from activation of biomass, which impacts not only nature of AC but also composition of bio-oil and gases in activation of biomass. In this study, activation of sawdust/cellulose/lignin directly or via pre-carbonization were conducted, with a particular focus on effects of pre-carbonization on evolution of the various products and environment. The results indicated that the pre-carbonization removed significant portion of aliphatic structures, creating difficulty for developing pores from the resulting biochar. This, however, enhanced overall yields of AC and gases at expense of bio-oil with K 2 C 2 O 4 , CO 2 or H 2 O as activators. The high yield and high specific surface area of AC could not be obtained simultaneously and needs to be balanced in application. In converse, ZnCl 2 or H 3 PO 4 as activators generated more AC with developed pore structures via direct activation of biomass, as they catalyzed the dehydration of aliphatic structures in biomass, but not in biochar. The life cycle assessment (LCA) indicated that the pre-carbonization led to a higher impact to environment and energy consumption than the direct activation. ZnCl 2 and CO 2 showed the smallest influence on the fossil fuel depletion and global warming. Direct activation exhibited lower environmental impact in the production of AC with more developed pore structures, rendering it a more desirable route for production of AC.
研究领域:
环境科学
·
材料科学
·
化学工程
·
可持续性
✓ 已验证
产品关联
DOI
高影响因子
Food & Function
·
2024
·Vol. 15
·期 11
·6068-6081
·
IF 5.1
Haonan Yu,Zhenyou Lou,Tingbo Wu,Xiaochun Wan,Haitao Huang,Yuanyuan Wu,Bo Li,Youying Tu,Puming He,Junsheng Liu
Epigallocatechin gallate (EGCG), a prominent bioactive compound found in tea, offers numerous health benefits. Previous studies have highlighted its potential in mitigating hyperuricemia. In this study, hyperuricemic mice induced by potassium oxonate (PO) were treated with EGCG or the anti-hyperuricemia medication allopurinol (AP) to investigate the mechanisms underlying their anti-hyperuricemic effects. The results demonstrated that both EGCG and AP significantly reduced serum uric acid (UA) levels. Further analysis revealed that EGCG promoted the expression of UA secretion transporter genes (Oat1 and Oct1) while inhibiting the expression of UA reabsorption transporter genes (Urat1 and Glut9) in the kidney. By 16S rDNA sequencing, EGCG, but not AP, was found to alter the composition of the gut microbiota. Notably, EGCG induced significant changes in the relative abundance of specific bacteria such as Lactobacillus, Faecalibaculum, and Bifidobacterium, which displayed high correlations with serum UA levels and UA-related gene expression. Metabolomic analysis suggested that EGCG-induced modifications in bacterial metabolites might contribute to the alleviation of hyperuricemia. Transcriptomic analysis of the intestinal epithelium identifies 191 differentially expressed genes (DEGs) in EGCG-treated mice, including 8 purine-related genes. This study elucidates the anti-hyperuricemic mechanisms of EGCG, particularly its influence on the gut microbiota and gene expression in the intestinal epithelium.
研究领域:
药理学
·
微生物学
·
分子生物学
·
营养学
·
转录组学
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
·
2023
·
IF 7.5
Xianghao Lin,Xiaojuan Zou,Baifei Hu,Dongyun Sheng,Tianxiang Zhu,Mingzhu Yin,Hui Xia,Haiming Hu,Hongtao Liu
This study aimed to evaluate the preventive effect of Bi Xie Fen Qing Yin (BXFQY) decoction on hyperuricemic nephropathy (HN). Using an HN mouse model induced by oral gavage of potassium oxonate and adenine, we found that BXFQY significantly reduced plasma uric acid levels and improved renal function. Further study shows that BXFQY suppressed the activation of the NLRP3 inflammasome and decreased the mRNA expressions of pro-inflammatory and fibrosis-associated factors in renal tissues of HN mice. Also, BXFQY prevented the damage to intestinal tissues of HN mice, indicative of suppressed colonic inflammation and increased gut barrier integrity. By 16 S rDNA sequencing, BXFQY significantly improved gut microbiota dysbiosis of HN mice. On the one hand, BXFQY down-regulated the abundance of some harmful bacteria, like Desulfovibrionaceae, Enterobacter , Helicobacter, and Desulfovibrio . On the other hand, BXFQY up-regulated the contents of several beneficial microbes, such as Ruminococcaceae, Clostridium sensu stricto 1 , and Streptococcus . Using gas or liquid chromatography-mass spectrometry (GC/LC-MS) analysis, BXFQY reversed the changes in intestinal bacterial metabolites of HN mice, including indole and BAs. The depletion of intestinal flora from HN or HN plus BXFQY mice confirmed the significance of gut microbiota in BXFQY-initiated treatment of HN. In conclusion, BXFQY can alleviate renal inflammation and fibrosis of HN mice by modulating gut microbiota and intestinal metabolites. This study provides new insight into the underlying mechanism of BXFQY against HN.
研究领域:
药理学
·
肾病学
·
微生物学
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）