CHEMOSPHERE
·
2023
·
IF 8.8
Xu Zhao,Kan Fu,Kai-ping Xiang,Lan-ying Wang,Yun-fei Zhang,Yan-ping Luo
Glufosinate-ammonium, the second largest transgene crop resistant herbicide, is classified as a mobile persistent pollutant by the U.S. Environmental Protection Agencybecause of its slow decomposition and easy mobile transfer in a water environment. The chronic and multigeneration toxicity of this compound to environmental organisms are alarming. In this study, racemic glufosinate-ammonium and the effective isomer, l - glufosinate-ammonium, were used as the test agents. The developmental, neurotoxic and reproductive toxicities of Caenorhabditis elegans to their parents and progeny were studied by continuous exposure in water at concentrations of 0.1, 1, 10 and 100 μg/L. The causes of toxicity differences were analysed from oxidative stress and transcription levels. Through oxidative stress of C. elegans , racemic glufosinate-ammonium and l - glufosinate-ammonium both mediated the developmental toxicity (shortened developmental cycle, reduced body length and width, promoted ageingand decreased longevity), neurotoxicity (inhibited head swinging, body bending frequency and acetylcholinesterase [AchE] activity) and reproductive toxicity (significant reductions in the number of eggs and offspring in vivo and induced apoptosis of gonadal cells). These phenomena caused oxidative damage (protein and membrane lipid peroxidation) and further induced apoptosis. The changes in various indicators caused by racemic glufosinate-ammonium exposure were more significant than those caused by l - glufosinate-ammonium exposure, and the reproduction-related indicators were more significant than the developmental and neurological indicators. A continuous accumulation of toxicity was observed after multiple generations of continuous exposure. These research results provide a data reference for the ecotoxicological evaluation and risk assessment of glufosinate-ammonium and contribute to the revision and improvement of the related environmental policies of glufosinate-ammonium.
研究领域:
生态毒理学
·
环境科学
·
毒理学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL TOXICOLOGY
·
2023
·
IF 4.5
Yuhong Kou,Yongping Chen,Tongtong Feng,Luomeng Chen,Hui Wang,Ning Sun,Shuping Zhao,Tianyuan Yang,Wenjing Jiao,Guofeng Feng,Honggang Fan,Yuan Zhao
Glufosinate-ammonium (GLA) is a widely used herbicide, but less research has been done on its harmful effects on non-target organisms, especially aquatic organisms. In this study, 600 adult zebrafish were exposed to different concentration of GLA (0, 1.25, 2.5, 5, 10, and 20 mg/L) for 7 days, and the livers were dissected on the eighth day to examine the changes in liver structure, function, oxidative stress, inflammation, apoptosis, and Nrf2 pathway, and finally to clarify the mechanism of GLA induced liver injury in zebrafish. The levels of alanine aminotransferase, aspartate aminotransferase, reactive oxygen species, malondialdehyde, inflammatory factors (IL-6 and TNF-α), and caspase-3 gradually increased, while the levels of superoxide dismutase, catalase, glutathione, and glutathione peroxidase gradually decreased with the increase of GLA concentration. The Nrf2 pathway was activated at low concentrations (1.25–5 mg/L) and significantly inhibited at high concentrations (10 and 20 mg/L). These results suggested that GLA could cause oxidative stress, inflammation, and apoptosis in zebrafish liver. Therefore, GLA can cause liver injury in zebrafish, and at high concentrations, the inhibition of Nrf2 pathway is one of the important causes of liver injury.
研究领域:
水生毒理学
·
环境科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2025
·
IF 3.6
Jin Li-Qun,Liu Meng-Dan,Guan Zi-Yu,Li Yi-Xin,Xue Ya-Ping,Liu Zhi-Qiang,Zheng Yu-Guo
DAAO is applied as a potential catalyst in the biosynthesis of L-PPT. However, its low solubility expression constrains its broader industrial application. Herein, a novel DAAO derived from Cladophialophora carrionii ( Cc DAAO) was identified, which demonstrated superior catalytic performance toward D-Ala (specific activity: 106.38 ± 1.21 U/mg, K m : 1.56 ± 0.06 mM), along with remarkable thermostability and broad substrate spectrum. Under optimal culture conditions, the soluble expression level of Cc DAAO was enhanced through a co-expression strategy with molecular chaperones, and the enzyme activity increased by 36.3% compared with the initial level. Subsequently, Cc DAAO was constructed as a fusion protein (CGD) with catalase from Geobacillus sp. CHB1 ( Gb CAT) and applied in a D-amino acid aminotransferase (DAAT)-mediated cascade system. In a 2 L reaction system, this cascade system achieved complete conversion (> 99%) of 1 M D,L-PPT within 8 h, exhibiting a yield of 11.26 g/L/h for PPO, which represents a significant improvement over existing reports. This study presents a promising practical approach for the industrial production of optically pure L-PPT.
研究领域:
生物催化
·
酶工程
·
药物合成
·
工业微生物
·
蛋白质工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Yong Wang,Wanjun Gao,Yeyun Li,Yu Xiao,Wei Song,Ting Yao,Manhuan Cheng,Wenjuan Wang,Ruyan Hou
The tea shrub is grown in long-standing orchards, an environment that is suitable for persistent weed growth, which is increasingly controlled by herbicides. Therefore, there is increasing concern that tea consumers may be exposed to herbicide residues. In this study, the levels of glufosinate-ammonium (GLU), glyphosate [N-(phosphonomethyl) glycine; PMG], and its metabolite aminomethyl phosphoric acid (AMPA) were determined in tea samples by HPLC–MS/MS using several current purification methods and a new method that we developed herein. The matrix effect of our proposed method was between -27.3 and 27.7%, which was lower than that in other methods, indicating that this method effectively reduced the interference of tea matrix in the mass spectrometry process. This method was used to determine the levels of PMG, GLU, and AMPA in 780 samples, including six traditional Chinese teas (green tea, black tea, oolong tea, dark tea, white tea, and yellow tea) and a floral tea, from 14 provinces of China. Probability estimates showed that the 95th percentile risk entropy values of the three pesticide residues were far below the acceptable risk level. The risk assessment results showed that exposure to PMG, GLU, and AMPA caused by drinking tea beverages poses no significant risk to human health.
研究领域:
分析化学
·
环境科学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2018
·
IF 4.358
Luyao Zhang,Jinling Diao,Li Chen,Zikang Wang,Wenjun Zhang,Yao Li,Zhongnan Tian,Zhiqiang Zhou
Glufosinate-ammonium (GLA) is a spectrum herbicide that is widely used in agriculture. The toxic effects of GLA on plants and mammals have been extensively studied; however, little is known about its effects on reptiles. In this study, male lizards ( Eremias argus ) were exposed to GLA contaminated soil for 60 days. Physical conditions, organ coefficients, antioxidant enzyme activity , tissue distribution, histopathological damage, steroid hormones levels, and related gene expression of sex steroids were evaluated. In contrast to unexposed control lizards, the body mass index of the GLA group was decreased, which elucidated that GLA adversely affected the physical condition of E. argus . Changes in antioxidant enzyme activities in response to elevated malondialdehyde levels in lizard testis indicated that testes were strongly affected by oxidative damage, and the increased testis index was associated with severe testis lesions. Moreover, alterations of plasma sex hormone levels and related gene expression levels of sex steroids were also observed, and the mechanism underlying the induction of reproductive toxicity was clarified. The activity of glutamine synthetase was severely inhibited in the liver of the GLA exposure group. Based on the results of liver index and histopathology examinations, the hepatotoxicity effect of GLA was confirmed.
研究领域:
环境毒理学
·
生殖生物学
·
生态毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Luyao Zhang,Li Chen,Zhiyuan Meng,Ming Jia,Ruisheng Li,Sen Yan,Sinuo Tian,Zhiqiang Zhou,Jinling Diao
In the context of global warming , an important issue is that many pesticides become more toxic, putting non-target organisms at higher risk of pesticide exposure. Eremias argus (a native Chinese lizard) was selected as animal model in this study. As a kind of poikilothermic vertebrate, E.argus is sensitive to temperature change. The experimental design [(with or without L-Glufosinate-ammonium (L-GLA) pollution × two temperatures (25 and 30 °C)] was used in this study for 90 days to identify the chronic effects of the pesticide–temperature interaction on the lizards’ neuroendocrine-regulated reproduction. Survival rate, body weight, clutch characteristics, testicular histopathology , the content of neurotransmitters and related enzyme activity , the level of sex steroid, the expression of Heat shock protein 70 (HSP70), antioxidant system, the accumulation and degradation of L-GLA were examined. Results showed that L-GLA disrupt reproduction of lizards through hypothalamus-pituitary-gonad (HPG) axis. In addition, temperature can not only change the environmental behavior of pesticides, but also alter the physiological characteristics of lizards. Thus, our results emphasized that temperature is an essential abiotic factor that should not be overlooked in ecotoxicological studies.
研究领域:
生态毒理学
·
环境科学
·
生殖生物学
·
神经内分泌学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DEVELOPMENTAL CELL
·
2024
·
IF 10.7
Xinyue Lu,Qiyan Zhang,Zejia Wang,Xuanzhi Cheng,Huiru Yan,Shuyi Cai,Huawei Zhang,Qikun Liu
Plants demonstrate a high degree of developmental plasticity, capable of regenerating entire individuals from detached somatic tissues—a regenerative phenomenon rarely observed in metazoa. Consequently, elucidating the lineage relationship between somatic founder cells and descendant cells in regenerated plant organs has long been a pursuit. In this study, we developed and optimized both DNA barcode- and multi-fluorescence-based cell-lineage tracing toolsets, employing an inducible method to mark individual cells in Arabidopsis donor somatic tissues at the onset of regeneration. Utilizing these complementary methods, we scrutinized cell identities at the single-cell level and presented compelling evidence that all cells in the regenerated Arabidopsis plants, irrespective of their organ types, originated from a single progenitor cell in the donor somatic tissue. Our discovery suggests a single-cell passage directing the transition from multicellular donor tissue to regenerated plants, thereby creating opportunities for cell-cell competition during plant regeneration—a strategy for maximizing survival.
研究领域:
植物生物学
·
发育生物学
·
分子遗传学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yaowei Zhu,Ruijie Tang,Jun Zhao,Changlong Jiang,Lingfei Li,Bianhua Liu
Tetracyclines antibiotics (TCs) are widely used as broad-spectrum antibiotics, but their overuse leads to environmental pollution and threatens human health. Existing detection techniques (mass spectrometry, etc.) are complicated and cumbersome, and do not allow for rapid detection. Consequently, it is imperative to develop a sensing technology for rapid on-site detection of full-spectrum TCs residues. The UV–visible absorption peaks of TCs overlap with the blue fluorescence excitation wavelength range, which can “turn-off” the blue fluorescence based on the internal filtering effect. Additionally, the β-diketone bond of TCs can bind to Eu 3+ , sensitizing Eu 3+ to produce red fluorescence. Thus, we constructed a selective sensing chemo-fluorescent sensor using blue nitrogen-doped carbon dots (BCDs) and europium metal–organic framework (Eu-MOF). As the concentration of TCs increased from 0 to 2.6 μM, the sensor exhibited a continuous change in fluorescence color from blue to purple to red. The limit of detection is 1.37 nM with a response time of less than 1 s. Furthermore, this study presents a portable capillary chemo-fluorescent sensor detection kit, which consists of a capillary chemo-fluorescent sensor comprising BCDs/Eu-MOF encapsulated in a self-designed non-fluorescent glass capillary, as well as a color recognizer with a kit produced using 3D printing technology. This fluorescence sensing technology with excellent sensitivity and selectivity provides technical support for the rapid detection of TCs and the reduction of pollutant hazards to sustainable human development.
研究领域:
分析化学
·
环境科学
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Xin Liu,Shiyue Niu,Bin Yang,Jia Liu,Liqian Niu,Xian Wang,Daqian Song,Shuyun Bi
Ultrasensitive Surface-enhanced Raman spectroscopy (SERS) method for the detection of diquat/dipterex was established using bovine serum albumin (BSA)-protected silver nanoparticles (AgNPs) modified MIL-53(Al) (named as BSA/MIL-53(Al)/AgNPs). Compared with unmodified AgNPs, BSA/MIL-53(Al)/AgNPs significantly enhanced the Raman signals of diquat and dipterex and the enhancement factors (EFs) were 1.58 × 10 7 and 2.34 × 10 7 , respectively. The TEM, XRD, TGA, XPS, UV–vis and FT-IR were utilized to characterize BSA/MIL-53(Al)/AgNPs and the binding of the substrate with diquat/dipterex. The optimal measurement conditions were investigated in detail by single factor experiment and response surface model. The impacts of common pesticides and coexisting substances on the determination of diquat/dipterex were studied. Under optimum conditions, linear calibration curves for detecting diquat/dipterex were established with a limit of detection (LOD) of 0.17/0.89 pmol L −1 (3 S 0 / S ). The SERS approaches were used to detect diquat and dipterex in several fruits and vegetables. The recovery was 97.10 %–104.82 % with the relative standard deviation (RSD) of 1.04 %–4.15 % ( n = 5).
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Plants-Basel
·
2025
·Vol. 14
·期 22
·
IF 4.1
Dong Li,Xumin Wang,Yujiao Song,Jianchao Sun,Shuhan Yu,Bowei Zhu,Xin Liu,Guodong Zhao,Tongsheng Zhao,Limin Wang
Seed oil content and fatty acid (FA) composition collectively determine the quality and economic value ofBrassica napus. Little is known about the role of R2R3-MYB transcription factors (TFs) in regulating FA biosynthesis inB. napus. Here,BnaC04.MYB89was found to be expressed primarily in developing seeds. Overexpression ofBnaC04.MYB89consistently decreased FA levels, as evidenced by its effect in both theArabidopsis thaliana myb89-1mutant andB. napusseeds. RNA-seq of developing seeds at 30 DAP (days after pollination) revealed marked suppression of FA biosynthetic genes inBnaC04.MYB89-overexpressing plants compared to the K407 control. ChIP (Chromatin immunoprecipitation) analysis revealed that BnaC04.MYB89 directly inhibited the expression ofBnaA03.BCCP1andBnaC03.HDwhile indirectly regulating that ofBnaA09.BADC1,BnaA03.BADC3,BnaA03.MOD1, andBnaA08.FAT8, thereby reducing seed FA accumulation. Collectively, these results elucidate the role for BnaC04.MYB89 and provide new insights into the transcriptional regulatory network controlling seed oil accumulation inB. napus.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLANT CELL AND ENVIRONMENT
·
2025
·
IF 6.3
Zhiquan Qiang,Zhen Zeng,Danfeng Ma,Jinzhe Li,Yuang Zhao,Tao Qin
Drought serves as a major environmental stress that restricts both the yield and quality of perennial ryegrass. Therefore, it is important to identify the essential genes that determine drought tolerance in perennial ryegrasses. In this paper, we isolated a drought-induced NAC transcription factor LpNAC22 . Transcriptional activity assays in yeast and plant cells indicated that LpNAC22 has transcriptional activation function. Subcellular localization observations revealed that LpNAC22 localized in the nucleus, compatible with its function as a transcription factor. LpNAC22 overexpression plants had enhanced drought tolerance and reduced cell membrane damage, whereas the knockdown of LpNAC22 in perennial ryegrass reduced plant drought tolerance and led to aggravated cell membrane damage. Late embryogenesis abundant (LEA), well-known stress resistance proteins, can protect the cell membrane from damage during drought conditions. DNA affinity purification sequencing and transcriptional regulation analysis demonstrated that LpNAC22 upregulates two LEA family genes, LpLEA1 and LpLEA2-1 , by directly binding to their promoters. Furthermore, we demonstrated that overexpression of LpLEA1 and LpLEA2-1 in Arabidopsis enhanced drought tolerance and reduced cell membrane damage under drought conditions. Our findings provide evidence that LpNAC22 improves drought resistance by modulating the transcription of LEA family genes in perennial ryegrass.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Dong Li,Xumin Wang,Tiantian Liu,Yaping Song,Yujiao Song,Shuhan Yu,Xin Liu,Han Wang,Yingjie Wang,Jianchao Cui,Limin Wang,Da Zhang,Hongxia Zhang
Arabidopsis R-R-type MYB-like transcription factors AtDIV1 and AtDIV2 (DIVARICATAs) act as critical participants in response to salinity stress. However, their functions in flowering transition are unknown. Here, we reported the function of AtDIV1 in flowering regulation. Protein sequence and 3D structure comparison showed that AtDIV1 was highly conserved, and contained two MYB-like DNA binding domains and one typical 'SHAQKYF/Y' motif. Expression pattern analyses exhibited that AtDIV1 was highly expressed at the bolting stage in Arabidopsis. Overexpression of AtDIV1 in Arabidopsis promoted the flowering of transgenic plants, whereas mutation of AtDIV1 postponed the flowering of div1-1 and div1-2 mutants. Transcriptome analyses revealed that a number of plant hormone and flowering associated genes were up- or down-regulated in transgenic plants. ChIP and EMSA analyses demonstrated that AtDIV1 directly bound to the promoter regions of TEM1 , SOC1 and GA3ox1 . Further growth experiments in AtDIV1 transgenic plants and div1-1 mutant demonstrated that the content of GA in them was respectively increased and decreased, and the promoted and postponed flowering of them could be respectively rescued with the exogenous application of GA 3 and GA biosynthesis inhibitor paclobutrazol. Our results provide insights into the regulatory mechanism of AtDIV1 mediated flowering via GA signalling pathway in Arabidopsis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）