SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2018
·
IF 2.88
Zuzhi Zhao,Fengwei Zhang,Zipin Zhang
Development of a technically simple yet effective method for paraquat (PQ) detection is of great importance due to its high clinical and environmental relevance. In this study, we developed a pyranine-based fluorescent “turn-off” method for PQ sensing based on pyranine-PQ interaction. We investigated the dependence of analytical performance of this method on the experimental conditions, such as the ion strength, medium pH, and so on. Under the optimized conditions, the method is sensitive and selective, and could be used for PQ detection in real-world sample. This study essentially provides a readily accessible fluorescent system for PQ sensing which is cheap, robust, and technically simple, and it is envisaged to find more interesting clinical and environmental applications.
研究领域:
分析化学
·
环境科学
·
临床化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Xingjie Wu,Hong Yang,Wei Li,Ling Tao,Qingteng Wang,Yingzu Jin,Xuecheng Sun,Xiangchun Shen
The prompt diagnosis of acute myocardial infarction (AMI) for patients with acute ischemic-type chest pain is critical for timely AMI treatment and lowering its casualty rate. In this article, we developed a multiparametric detection technique for AMI related microRNAs (miRNAs), which is based on the polymerization induced fluorescence-off (PIFO) signal amplification technique. PIFO can be implemented on a specially designed microfluidic chip equipping a fluorescence sensor, which is able to profile 3 miRNAs simultaneously in 45 min with a detection limit of 6100 miRNAs copies. Comparing with other polymerization-based signal amplification techniques, PIFO is rapid, oxygen-independent, multiparametric, and can be performed in ambient environment. The miniaturized PIFO device can be further applied to analyze AMI related miRNAs in clinical serum samples, and displays excellent performance in distinguishing AMI patients from patients with other cardiovascular diseases. Moreover, PIFO shows a high accuracy for AMI diagnosis among patients with low hypersensitivity troponin I level (< 0.1 ng mL −1 ) as a result of the early and fast respond to AMI onset by miRNAs.
研究领域:
医学诊断
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分子生物学
·
生物传感器
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Jian Zhu,Hui Chang,Jian-Jun Li,Xin Li,Jun-Wu Zhao
Both “turn-on” and “turn-off” fluorescent probe for the determination of melamine have been developed by tuning the inner filter effect (IFE) from CdTe quantum dots (QDs) to gold nanoparticles. Because of the melamine-induced aggregation of gold nanoparticles, the first absorption peak of the transverse surface plasmon resonance (SPR) at 520 nm fades down, whereas the second absorption peak of the longitudinal SPR around 620 nm gets intense. The decrease of the transverse SPR reduces the IFE of the bundled CdTe QDs with an emission band at 525 nm (the corresponding particle size is 1.98 nm), which results in the melamine concentration-dependent fluorescence recovery of CdTe QDs. The increase of the longitudinal SPR enhances the IFE of the bundled CdTe QDs with an emission band at 620 nm (the corresponding particle size is 3.68 nm), which results in the melamine concentration-dependent fluorescence decrease of CdTe QDs. The green mode detection with the fluorescence recovery has a linear response for melamine from 20 to 100 ng/mL with a theoretical detection limit of 8 ng/mL. The red mode detection with the fluorescence decrease has a linear response for melamine from 20 to 100 ng/mL with a theoretical detection limit of 11 ng/mL. Interference studies and real samples detection show that the melamine could be specifically detected using this dual-mode fluorescent probe.
研究领域:
分析化学
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纳米技术
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环境科学
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食品安全
✓ 已验证
DOI
高影响因子
ORGANIC & BIOMOLECULAR CHEMISTRY
·
2016
·Vol. 14
·期 9
·2684-2690
·
IF 3.559
Kui Wang,Jian-Hua Cui,Si-Yang Xing,Hong-Xi Dou
We have successfully implemented the supramolecular tandem assay principle for the real-time, continuous, direct, and label-free monitoring of alkaline phosphatase activity through a fluorescence “switch-off” assay based on a novel calixpyridinium/dye reporter pair. Because several diseases can be preliminarily diagnosed in light of an abnormal level of alkaline phosphatase in serum, the application of tandem assays to selectively monitor alkaline phosphatase activity has feasible implications in disease diagnosis.
研究领域:
生物化学
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分析化学
·
诊断学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Siyang Deng,Huan Liu,Chunhui Zhang,Xinting Yang,Christophe Blecker
Nitrite (NO 2 − ) is widely present in meat products as a vital food preservative, while excessive nitrite is a serious carcinogen to human beings, developing a sensitive and selective nitrite detection method is of great significance for food safety. Herein, a novel luminescent metal organic framework (LMOF) (Rh6G@MOF-5) is prepared through a facile one-pot synthesis as a fluorescent turn-off sensor for NO 2 − determination. Upon exposure to NO 2 − , significant fluorescence intensity decreases linearly along with NO 2 − concentration in the range of 0–200 μmol/L and the detection limit is estimated to be 0.2 μmol/L. The developed nanocomposite has been shown to be an ultrasensitive and selective nanoprobe for detecting NO 2 − in meat products, with recoveries ranging from 96.1% to 103.2% with the RSDs less than 4%. Rh6G@MOF-5 nanoprobe offers insight into the fabrication of MOF-based sensors with low cost and convenient operation in analyte detection field.
研究领域:
食品科学
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分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）