TALANTA
·
2025
·
IF 6.1
Yifei Ma,Lijie Zhang,Hong Yang,Shanshan Zhu,Jinhua Liu
Highly selective and sensitive determination of dopamine (DA) from multiple sources remains a persistent and significant challenge. Here, we develop an imidazole-triggered in situ fluorescence reaction system for highly selective and sensitive determination of DA from various sources (human, horse, dog, rabbit, and mouse). The system operates by catalyzing the oxidation of DA with 1,5-Dihydroxynaphthalene (1,5-DHA) through a Lewis base formed by imidazole, leading to the rapid generation of yellow azamonardine fluorescent compounds (AFC). Notably, the system demonstrates minimal background noise and a high signal-to-noise ratio of up to 300-fold with a determination limit of 33.33 pM, making it 10-100 times more sensitive than conventional enzyme-linked immunosorbent assay (ELISA) methods. Moreover, selectivity tests reveal that our system can effectively distinguish between several common interfering substances, even at concentrations as low as 10 nM. The developed system shows promising results in detecting DA from diverse sources (humans, horses, dogs, rabbits, and mice), including urine samples from clinical patients, exhibiting good agreement with traditional ELISA kits. Therefore, the established in situ fluorescence reaction system holds great potential for the determination of DA-related disorders due to its impressive analytical capabilities.
研究领域:
分析化学
·
神经生物学
·
医学检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2020
·
IF 0.936
Jia-Hui ZHAO,Guo-Yong LIU,Shuang WANG,Sha-Sha LU,Jian SUN,Xiu-Rong YANG
Dopamine is a crucial monoamine neurotransmitter involved in multiple cognitive functions and pathological processes. Herein, we described a rapid chromogenic and fluorogenic reaction between dopamine and naphthoresorcinol under ambient conditions, and found that the product possessed intense visible absorbance and high fluorescence quantum yield (78%) in aqueous solution. Through electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) analysis, the plausible reaction mechanism between dopamine and naphthoresorcinol was deduced, and the structure of the reaction product was confirmed. Inspired by the explicit mechanism and accessible reaction conditions, a straightforward and selective assay for dopamine concentration was developed with colorimetric and fluorescent dual-modal signals based on the in situ reaction.
研究领域:
生物化学
·
分析化学
·
神经科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL PAPERS
·
2022
·Vol. 76
·期 7
·4235-4244
·
IF 2.146
Ding Yu,Yang Li-Hong,Shen Ji-Wei,Wei Yin-Mao,Wang Chao-Zhan
In this work, simple, cost efficient, highly selective and sensitive assay methods for dopamine (DA) concentration and tyrosinase (TYR) activity were established based on a benzoyl peroxide (BPO) facilitated in situ “turn-on” fluorogenic reaction between resorcinol (RS) and DA, in which BPO was introduced in the system to accelerate DA oxidation, thereby realizing a faster reaction with RS to form the fluorescent substance, azamonardine, which was verified by HPLC, 1 H NMR, 13 C NMR and high resolution mass spectrometry. The introduction of BPO remarkably improved the sensitivity, linearity range and response speed for assay of DA and TYR. For the determination of DA concentration, the concentration has a good linear relationship in the range of 4.0–1.2 μM, and the detection limit is as low as 0.8 nM, and it was successfully applied to measuring DA in human urine and serum samples. In addition, a fluorescent method for determining the TYR activity was further established based on the enzymatic oxidation of tyramine to DA, the detection limit is 0.0013 U mL −1 . And the method was used to determine TYR in human serum and screening of TYR inhibitors.
研究领域:
生物化学
·
医学检验
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Jiahui Zhao,Guoyong Liu,Jian Sun,Qifeng Wang,Zong-Jun Li,Xiurong Yang
Analyte-responsive chromo-fluorogenic reactions under accessible conditions are important for designing small-molecule spectroscopic probes. We describe a series of newly constructed motifs based on the chromo-fluorogenic reaction between catechol derivatives (typically hydroxytyrosol, dopamine, and levodopa) and naphthoresorcin (NR) in aqueous solution under ambient conditions. The weakly absorptive and fluorogenic catechols/NR was converted to products having visible absorption and bright fluorescence within several minutes. The chromo-fluorophores produced from this reaction had a maximum absorbance at 458 nm and emission at 480 nm with high fluorescence quantum yields (30–84%). Inspired by the tyrosinase-catalyzed hydroxylation of monophenols to catechols, the tyrosinase-enabled chromo-fluorogenic reaction was verified by using monophenol (typically tyrosol) as the substrate. In this regard, a dual-readout tyrosinase activity assay was developed by virtue of the in situ “turn-on” optical signals. Furthermore, a test of tyrosinase inhibition, by using a common inhibitor kojic acid, demonstrated the potential of the chromo-fluorogenic reaction for developing other tyrosinase related assays and signal transduction.
研究领域:
生物化学
·
分析化学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Zhiyan Chen,Wen-Tao Wang,Wenchao Wang,Jinsong Huang,Jia-Yu Liao,Su Zeng,Linghui Qian
Owing to its important biological functions, RNA has become a promising molecular biomarker of various diseases. With a dynamic change in its expression level and a relatively low amount within the complicated biological matrix, signal amplification detection based on DNA probes has been put forward, which is helpful for early diagnosis and prognostic prediction. However, conventional methods are confined to cell lysates or dead cells and are not only time-consuming in sample preparation but also inaccessible to the spatial–temporal information of target RNAs. To achieve live-cell imaging of specific RNAs, both the detection sensitivity and intracellular delivery issues should be addressed. Herein, a new cascaded fluorogenic system based on the combination of hybridization chain reactions (HCRs) and proximity-induced bioorthogonal chemistry is developed, in which a bioorthogonal reaction pair (a tetrazine-quenched dye and its complementary dienophile) is brought into spatial proximity upon target RNA triggering the HCR to turn on and amplify the fluorescence in one step, sensitively indicating the cellular distribution of RNA with minimal false positive results caused by unspecific degradation. Facilitated by a biodegradable carrier based on black phosphorus with high loading capacity and excellent biocompatibility, the resulting imaging platform allows wash-free tracking of target RNAs inside living cells.
研究领域:
分子生物学
·
生物医学工程
·
分析化学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2020
·Vol. 13
·期 4
·1008-1016
·
IF 2.667
Zha Yonghong,Liu Xiaolei,Hu Pan,Lu Shiying,Ren Honglin,Liu Zengshan,Liang Xiongyan,Yang Yuying,Li Yansong,Zhou Yu
Enzyme-linked immunosorbent assay (ELISA), as an immunoassay platform, usually suffers from poor sensitivity due to the limited signal intensity. To overcome this obstacle, an alkaline phosphatase–triggered immunoassay based on fluorogenic reaction of o -phenylenediamine and ascorbic acid was developed for sensitive detection of chloroacetamide herbicides in corn. The new immunoassay revealed limits of detection (LODs) for acetochlor (ATC), metolachlor, and propisochlor of 1.19 ng/mL, 2.34 ng/mL, and 3.40 ng/mL, respectively. The average recoveries of the three chloroacetamide herbicides in corn sample were 88.4%, 91.8%, and 87.6%, respectively. Such fluorescent immunoassay via in situ formation of fluorescent signal from scratch provides a new strategy with excellent sensitivity in point-of-care test of chloroacetamide herbicides.
研究领域:
分析化学
·
环境科学
·
农业科学
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Yuexin Pang,Zerui Ma,Qian Song,Zhenguang Wang,Yu-e Shi
A fluorogenic reaction between the chelate of Mn(II)-citric acid and terephthalic acid (PTA) was discovered, which was carried out through heating the aqueous mixture of Mn 2+ , citric acid and PTA. Detailed investigations indicated the reaction products were 2-hydroxyterephthalic acid (PTA-OH), which was attributed to the reaction between PTA and OH, formed by the triggering of Mn(II)-citric acid in the presence of dissolved O 2 . PTA-OH showed a strong blue fluorescence, peaked at 420 nm, and the fluorescence intensity presented a sensitive response to pH of the reaction system. Based on these mechanisms, the fluorogenic reaction was used for the detection of butyrylcholinesterase activity, achieving a detection limit of 0.15 U/L. The detection strategy was successfully applied in human serum samples, and it was also extended for the detection of organophosphorus pesticides and radical scavengers. Such a facile fluorogenic reaction and its stimuli-responsive properties offered an effective tool for designing detection pathways in the fields of clinical diagnosis, environmental monitoring and bioimaging.
研究领域:
生物化学
·
临床诊断
·
环境监测
·
生物成像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）