MOLECULES
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2018
·Vol. 23
·期 9
·2290
·
IF 3.098
Liyan Liu,Yaohui Lei,Jianhui Zhang,Na Li,Fan Zhang,Huaqiao Wang,Feng He
Fluorophores with aggregation-induced emission enhancement (AIEE) properties have attracted more attention in recent years. In order to realise more valuable applications, the different kinds of AIEE molecules are in serious need of further development. Therefore, a novel flavone-based AIEE system derived from restriction of intramolecular rotation (RIR) was designed and synthesized in this work. The results revealed that six of the compounds showed typical AIEE characteristics, with fluorescence emissions from purple, blue, cyan to green, tunable by changing substituent groups. This flavone-based AIEE system has never been reported before. The AIEE characteristics were investigated by optical spectroscopy, fluorescence photographs, scanning electron microscopy (SEM), fluorescence quantum yields (ФF) and fluorescence lifetime in the CH3OH/H2O mixed solution. Moreover, benefiting from the simple structures and small molecular weight, they could permeate cells faster than current high-molecular-weight AIEE molecules. Furthermore, to examine possible biomedical applications, fluorescence imaging in living A549 lung cells and cell viabilities were examined, and the results displayed that these fluorophores showed good cellular uptake and low cytotoxicity within the experimental concentration range. In addition, these AIEE compounds possessed excellent specificity for mitochondrial targeting and mitochondrial morphological change tracking, besides, they displayed superior photostability, which indicated they are potential candidates for mitochondrial imaging.Keywords:AIEE;multicolor;flavone;fluorophores;mitochondria
研究领域:
化学
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材料科学
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生物医学工程
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细胞生物学
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DOI
PubMed
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 59
·34652-34657
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IF 3.049
Shengling Li,Duanlin Cao,Zhiyong Hu,Zhichun Li,Xianjiao Meng,Xinghua Han,Wenbing Ma
In this study, a highly selective chemosensor ML based on a BODIPY fluorescent chromophore was synthesized for sequential recognition of Cu2+ and HSO3− in a CH3OH/H2O (99 : 1 v/v) system, which contained three recognition sites and its structure characterized by 1H NMR, 13C NMR and ESI-HR-MS. The sensor ML showed an obvious “on–off” fluorescence quenching response toward Cu2+ and the ML-Cu2+ complex showed an “off–on” fluorescence enhancement response toward HSO3−. The detection limit of the sensor ML was 0.36 μM to Cu2+ and 1.4 μM to HSO3−. In addition, the sensor ML showed a 1 : 3 binding stoichiometry to Cu2+ and the recovery rate of ML-Cu2+ complex identifying HSO3− could be over 70%. Sensor ML showed remarkable detection ability in a pH range of 4–8.
研究领域:
分析化学
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生物化学
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DOI
高影响因子
DYES AND PIGMENTS
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2021
·
IF 4.889
Peng Wu,Yuan Xiong,Chen Lei,Yuan Li,Xiang Liu,Cheng Zhang,Yudie Sun,Jian Zhang,Chun-Sing Lee,Kui Zhang
Carbon dots or Carbon quantum dots (herein after abbreviated as CDs) with bight luminescence, derived from carbohydrates such as citric acid , glucose or sucrose, have been widely reported in recent publications. However, as to which functional groups and structures in CDs are responsible for luminescence, most explanations in current references are normally based on assumptions, instead of being convinced by the accurate information resting on experimental facts. Herein, we conducted an experimental study targeting at finding the fluorophores of CDs prepared from citric acid and glycine by fluorescent quenching. The experimental results revealed that the essence of fluorescence was manipulated by fluorophores of conjugated C C double bonds and aldehyde groups. The destruction of aldehyde groups or C C double bonds in the CDs led to a complete decay of luminescence. In addition, the results of this experiment were similar to those of CDs prepared from the sole citric acid, glucose or sucrose, which predicted the analogy of the CDs in chemical structures . Such information about fluorophore of CDs was valuable for further exploring more extensive application of CDs in sense technologies.
研究领域:
材料科学
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化学
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DOI
高影响因子
Analytical Methods
·
2020
·Vol. 12
·期 12
·1617-1623
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IF 2.596
Guoqiang Liu,Hongwei Ge,Ranhao Yin,Long Yu,Congming Sun,Weiru Dong,Zhenli Sun,Khalid A. Alamry,Hadi M. Marwani,Suhua Wang
To discriminate hydrogen sulfide (H2S) from thiol nucleophiles with similar reactivity is always a major challenge in developing new detection methods. In this paper, we report a colorimetric and ratiometric fluorescence probe selectively reactive to H2S, which was synthesized by hybridizing the fluorescent nitro-benz-2-oxa-1,3-diazole (NBD)-based derivative P-NBD with carbon dots (CDs). The selective reaction can be monitored by distinct ratiometric fluorescence or colorimetric variation. The nanohybrid probe exhibited two fluorescence bands centered at 440 nm and 543 nm, respectively. The yellow fluorescence at 543 nm from P-NBD is quenched by H2S, while the blue fluorescence is enhanced by weakening of the Förster resonance energy transfer (FRET) mechanism. It was observed that the apparent fluorescence color evolved from yellow to blue when exposed to different amounts of H2S, accompanied by the color change of the probe solution from yellow to red under natural light. The detection limit was estimated to be 57 nM in aqueous solution. We successfully applied this probe for the detection of H2S in real water samples, suggesting its potential application in environmental monitoring and protection.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
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2015
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IF 6.723
Xuehong Min,Yuan Zhuang,Zhenyu Zhang,Yongmei Jia,Abdul Hakeem,Fuxin Zheng,Yong Cheng,Ben Zhong Tang,Xiaoding Lou,Fan Xia
We demonstrate an ultrasensitive microRNA detection method based on an extremely simple probe with only fluorogens but without quencher groups. It avoids complex and difficult steps to accurately design the relative distance between the fluorogens and quencher groups in the probes. Furthermore, the assay could accomplish various detection limits by tuning the reaction temperature due to the different activity of exonuclease III corresponding to the diverse temperature. Specifically, 1 pM miR-21 can be detected in 40 min at 37 °C, and 10 aM (about 300 molecules in 50 μL) miR-21 could be discriminated in 7 days at 4 °C. The great specificity of the assay guarantees that the real 21 urine samples from the bladder cancer patients are successfully detected by our method.
研究领域:
癌症研究
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分子生物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Chinese Physics B
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2021
·Vol. 30
·期 9
·097802
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IF 1.494
Guohua Cao,Zhifei Wei,Yuehong Yin,Lige Fu,Yukun Liu,Shengli Qiu,Baoqing Zhang
In citric acid-based carbon dots, molecular fluorophore contributes greatly to the fluorescence emission. In this paper, the nitrogen and sulfur co-doped carbon dots (N,S-CDs) were prepared, and an independent sulfur source is selected to achieve the doping controllability. The influence of sulfur doping on the molecular fluorophore was systematically studied. The introduction of sulfur atoms may promote the formation of molecular fluorophore due to the increased nitrogen content in CDs. The addition surface states containing sulfur were produced, and S element exists as –SO3, and –SO4 groups. Appreciate ratio of nitrogen and sulfur sources can improve the fluorescence emission. The photoluminescence quantum yields (PLQY) is increased from 56.4% of the single N-doping CDs to 63.4% of double-doping CDs, which ascribes to the synergistic effect of molecular fluorophores and surface states. The sensitivity of fluorescence to pH response and various metal ions was also explored.
研究领域:
材料科学
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光学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
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2021
·
IF 9.229
Xuanying Chen,Jiacheng Chen,Guangchen Sun,Lifang Guo,Jianhua Su,Zhiyun Zhang
Herein, a molecular pixel system for full-color luminescence reproduction is achieved by adjusting the colorless mixtures of two matching fluorophores, i.e., polarity-insensitive 9,14-diphenyl-9,14-dihydrodibenzo[a,c]phenazine (DPAC) as the fixed red primary color and polarity-sensitive dansylamide (DSA) as dynamic blue to green primary colors. DPAC and DSA possess independent emission properties free from electron and energy transfer crosstalk between them because of their close frontier molecular orbitals as well as similar absorptions below 400 nm. According to the additive color theory, under diverse mixing ratios and various polarities, a smooth emission color change is realized in the triangle surrounded by the luminophores in the chromaticity diagram with accurate prediction and expedient reproduction. The principle of this system may open an innovative route for the development of powerful full-color luminescent materials, for example, ratiometric fluorescent polarity sensors and invisible fluorescent crayons.
研究领域:
化学
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材料科学
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DOI
高影响因子
Current Protocols
·
2021
·Vol. 1
·期 10
·e274
·
IF 0
Jingyi Wang,Jiachen Shang,Yu Xiang,Aijun Tong
Functionalized oligonucleotides (ONs) are widely applied as target recognition molecules for biosensing and gene regulation. Herein, we describe a general method for post-synthetic modification of ONs based on the oxidative amination of 4-thio-2′-deoxyuridine (4SdU) with sodium periodate and several amines. Alkyne-/azide-, biotin-, and fluorophore-modified ONs were prepared by modifying 4SdU-containing ONs with the corresponding amines and characterized for their bioorthogonal reactivity, streptavidin-binding affinity, and fluorescence properties, respectively. We synthesized three fluorophore-modified ONs with and without the aromatic fluorophores conjugated to modified nucleobases and investigated their emission properties. © 2021 Wiley Periodicals LLC. Basic Protocol 1 : Post-synthetic modification of ONs Supporting Protocol : Preparation of dansyl ethylenediamine Basic Protocol 2 : Quantification of ON reaction yields Basic Protocol 3 : Characterization of modified ONs
研究领域:
生物化学
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分子生物学
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化学生物学
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DOI
TETRAHEDRON
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2019
·
IF 2.379
Zhengzhou Liu,Zheng Lu,Weifeng Peng,Yang Liu,Jianghuai Hu,Ke Zeng,Gang Yang
In this paper, an adenine-derived fluorescent chromophore (HCC-APA) was synthesized to detect its interaction with iron ions . The results showed that a large stokes shift ( υ = 10981.5 cm −1 ) fluorescence of the HCC-APA was appeared. The fluorescence quantum yields of HCC-APA in DMF and THF were 3.49% and 17.54%, respectively, demonstrating its higher fluorescence performance than unmodified adenine . Besides, the fluorescence quenching of HCC-APA with iron ions was further detected. Fluorescence titration experiments confirmed that the detection limit of HCC-APA for Fe 3+ was 3.69 μM. The interaction between iron ions and HCC-APA was investigated by continuous variable method, indicating that the binding ratio is 1:1. And the interaction site was deeply explored by 1 H NMR and molecular simulation.
研究领域:
化学
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生物学
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DOI
高影响因子
TETRAHEDRON
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2020
·
IF 2.233
Jian Chen,Kai Chen,Baichuan Han,Yunting Xue,Wanjuan Chen,Zhenzhong Gao,Xianfeng Hou
A single-fluorophore-based ratiometric fluorescent probe based on anthracene carboximide was developed for detection of formaldehyde. In the presence of formaldehyde, the fluorescence emission would change from red to yellow-green, and the probe showed excellent sensitivity, selectivity and anti-interference. Furthermore, the response mechanism was illustrated to be that change in fluorescent signal is resulted from variation of substituent group on the 6th position of anthracene ring. A test paper was developed by loading probe onto filter paper strip. With this test paper, the detection of gaseous formaldehyde could be carried out conveniently, visually and quickly. 2009 Elsevier Ltd. All rights reserved.
研究领域:
分析化学
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环境科学
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生物医学
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产品关联
DOI
高影响因子
DYES AND PIGMENTS
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2021
·
IF 4.889
Selvaraj Muthusamy,Long Zhao,Kanagaraj Rajalakshmi,Dongwei Zhu,Rodah Soy,John Mack,Tebello Nyokong,Shengjun Wang,Kang-Bong Lee,Weihua Zhu
Tracking the biothiol cysteine (Cys) in living systems is a significant responsibility to balance the redox environment and oxidative stress. A quinoline-7-nitro-1,2,3-benzoxadiazole ( Q-NBD ) fluorophore has been synthesized and characterized towards examination of Cys. The probe forms a quinoline-substituted phenol ( Q-Ph-OH ) after thiolysis of the NBD ether bond, leading to an increase of fluorescence at green channel. The turn-on sensing mechanism originates from the change in intramolecular charge transfer (ICT-OFF) along with an aggregation-induced emission (AIE) as suggested by spectroscopy measurements in solutions, time-dependent density-functional theory (TD-DFT) calculations and 1 H NMR titration examination. Importantly, Q-NBD exhibited great sensitivity with a low limit of detection value of 89.5 nM and remarkable selectivity in various biothiols towards Cys. The sensor probe was successfully used for detecting both endogenous and exogenous Cys in PC3 living cells and spiked Cys in human urine samples.
研究领域:
生物化学
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分子生物学
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分析化学
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DOI
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2015
·Vol. 33
·期 8
·939-947
·
IF 1.578
Guo-Feng Zhang,Tao Chen,Ze-Qiang Chen,Matthew P. Aldred,Xianggao Meng,Ming-Qiang Zhu
We have synthesized a series of fluorene-based fluorophores, in which a central fluorene core has been modified with different peripheral arylene vinylene substituents that are able to activate aggregation-induced emission (AIE) characteristics. 9,9-Dioctylfluorene doubly end-capped at the 2,7-positions with triphenylethene groups, such as 4-(2,2-diphenylvinyl)phenyl (F1-(2,2)-HTPE) and 4-(1,2-diphenylvinyl)phenyl (F1-(1,2)-HTPE) were synthesized and compared to the tetraphenylethene analogue (F1-TPE). Both F1-(2,2)-HTPE and F1-(1,2)-HTPE glow with a deep blue fluorescence in THF solution with emission maxima ( λ em ) of 426 and 403 nm, respectively. The λ em slightly red-shifts in the solid-state to 458 nm for F1-(2,2)-HTPE and 437 nm for F1-(1,2)-HTPE. The fluorescence quantum yields ( ø F ) of F1-(2,2)-HTPE ( ø F =35.1 %) and F1-(1,2)-HTPE ( ø F =26.2%) were found to be higher in solution compared to the near quenching of F1-TPE ( ø F =0.1%). Consequently, this results in weaker AIE-stability of F1-(2,2)-HTPE ( α AIE =1.5) and F1-(1,2)-HTPE ( α AIE =1.9) compared to F1-TPE ( α AIE =125), suggesting that four phenyl groups are necessary for efficient AIE-activity of these fluorene bridged arylene vinylene type materials. In addition, decreasing the steric hindrance around the arylene vinylene moiety by removal of a phenyl ring is another method to decrease the AIE characteristics, in a similar manner to the commonly known "phenyl-locking". Non-polar triphenylethenes are poorer AIE materials than their tetraphenylethene analogues. Replacing the hydrogen atom of F1-(2,2)-HTPE with a cyano group affords fluorene end-capped with 2,3,3-triphenylacrylonitrile (F1-TPAN), which boosts the AIE-effect to α AIE =90.5 and red-shifts the solid-state emission ( λ em =528 nm) with near quenching in THF solution ( ø F =0.12%). X-ray crystallographic analysis of F1-TPAN indicates that the introduction of cyano groups can not only diminish the intramolecular steric hindrance in comparison of F1-TPE, but also improve the molecular cohesion ability via multiple C H···N interactions.
研究领域:
有机化学
·
材料科学
·
光学
✓ 已验证
DOI
高影响因子
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