MACROMOLECULAR BIOSCIENCE
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2018
·Vol. 18
·期 6
·1800045
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IF 3.392
Xi Yang,Shihong Shen,Li Guo,Jidong Tan,Henxin Lei,Jianghan Wu,Lei Zhao,Tao Xiong,Youshen Wu,Yilong Cheng,Yanfeng Zhang
The development of inexpensive and highly efficient enzyme-responsive polymers has significantly contributed to targeted drug delivery systems. Here, a superamphiphile with a capability of fluorescent dissociation sensing is designed. It is constructed with negatively charged adenosine 5′-triphosphate (ATP) and negatively charged fluorescein diphosphate (FDP), which are used as fluorescence detection, and a cationic diblock copolymer methoxy-poly(ethylene glycol) 113 - b -poly(2-dimethyl-aminoethyl methacrylate) 70 . Upon addition of calf intestinal alkaline phosphatase, the superamphiphile disintegrates, presumably due to the enzymatic hydrolysis of ATP. This process is accompanied by an increase in the fluorescence emission intensity of fluorescein owing to the hydrolysis of FDP. The in vitro application of the superamphiphile is also proven. Thus, the “turn-on” fluorescence of the superamphiphile serves as a real-time module for detection of the disintegration of superamphiphile.
研究领域:
生物材料学
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药物化学
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生物医学工程
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DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2015
·
IF 3.966
Kangnan Wang,Lin Ma,Guoqun Liu,Duxia Cao,Ruifang Guan,Zhiqiang Liu
Two novel diethylaminecoumarin Schiff's base chemosensors for cyanide with pyridine or thiophene as terminal group have been synthesized. Their photophysical properties as well as recognition properties for cyanide anions have also been examined. Both the compounds exhibit strong green fluorescence in all the solvents. Density functional theory calculation indicates that both the compounds exhibit intramolecular charge transfer from diethylaminecoumarin group to terminal pyridine or thiophene group. The compounds can recognize cyanide anions with fluorescence turn-on response based on copper ion complex displacement mechanism and obvious color change from red to yellow-green can be observed by naked eyes. The chemosensors exhibit a degree of repeatability and can be used repeatedly to recognize cyanide anions for several times.
研究领域:
分析化学
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生物化学
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高影响因子
Chemistry-An Asian Journal
·
2012
·Vol. 8
·期 1
·276-281
·
IF 4.5
Jian Chen,Huping Jiao,Wenying Li,Dongli Liao,Huipeng Zhou,Cong Yu
Graphical A desired breakdown : A novel tetracationic perylene probe (probe 1 ) was designed and synthesized. Probe 1 was used for the real-time fluorescence turn-on assay of alkaline phosphatase (ALP) activity and inhibitor screening. ALP triggered the degradation of ATP, the breakdown of the ATP/probe 1 complex, and the recovery of the probe 1 monomer fluorescence. en A tetracationic perylene probe (probe 2 ) was designed and synthesized. Probe 1 was used for the real-time fluorescence turn-on assay of alkaline phosphatase (ALP) activity and inhibitor screening. Probe 1 monomer fluorescence could be very efficiently quenched by ATP through the formation of an ATP/probe 1 complex. ALP triggered the degradation of ATP, the breakdown of the ATP/probe 1 complex, and the recovery of the probe 1 monomer fluorescence. In the presence of an ALP inhibitor, a decrease in fluorescence recovery was observed. zh 1
研究领域:
分析化学
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生物化学
·
分子生物学
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PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Shuya Wang,Yipeng Huang,Linchun Zhang,Feiming Li,Fangyuan Lin,Yiru Wang,Xi Chen
Highly fluorescent methylammonium lead bromide (MAPbBr 3 ) perovskite could in situ grow in a sulfydryl functionalized mesoporous alumina film when methylammonium bromide (MABr) meets the sulfydryl-captured Pb(II). In this work, the extraction ability of the mesoporous alumina film towards Pb(II) was found to drastically enhanced after sulfydryl modification. Under the optimal conditions, the Pb(II) adsorption capacity of the sulfydryl functionalized film was 94.9 μg/g. With the strong extraction capability to Pb(II), fluorescence turn-on determination of Pb(II) was enabled by in-situ formation of MAPbBr 3 perovskite in the sulfydryl functionalized alumina film, and a limit of detection (LOD) as low as 5 × 10 −3 μg/mL was achieved. This method integrates the extraction and background-free determination of Pb(II), well addressed the low concentration determination issue and facilitating the whole analysis processes.
研究领域:
分析化学
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环境科学
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Tianhong Wu,Shiqing Huang,Xing Feng,Xiaogang Liu,Tony D. James,Xiaolong Sun,Xuhong Qian
In this research, we developed a photoluminescent platform using amine-coupled fluorophores, generated from a single conjugate acceptor containing bis-vinylogous thioesters. Based on the experimental and computational results, the fluorescence turn-on mechanism was proposed to be charge separated induced energy radiative transition for the amine-coupled fluorophore, while the sulfur-containing precursor was not fluorescent since the energy internal conversion occurred through vibrational 2RS– (R represents alkyl groups) as energy acceptor(s). Further utilizing the conjugate acceptor, we establish a new fluorogenic approach via a highly cross-linked soft material to selectively detect cysteine under neutral aqueous conditions. Turn-on fluorescence emission and macroscopic degradation occurred in the presence of cysteine as the stimuli, which can be visually tracked due to the generation of an optical indicator and the cleavage of linkers within the matrix. Furthermore, a novel drug delivery system was constructed, achieving controlled release of sulfhydryl drug (6-mercaptopurine) which was tracked by photoluminescence and high-performance liquid chromatography. The photoluminescent molecules developed herein are suitable for visualizing polymeric degradation, making them suitable for additional “smart” material applications.
研究领域:
生物化学
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材料科学
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药物递送
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2021
·
IF 4.098
Kaiyu Zhang,Yuxin Sang,Yingdi Gao,Qinxing Sun,Weina Li
In this paper, a simple and effective fluorescence turn-on approach for highly sensitive and selective monitoring Hg 2+ ions was designed by using carbon dots (CDs) and silver nanoparticles (AgNPs). It reveals that the fluorescence of CDs solution can be quenched in the presence of AgNPs through inner filter effect (IFE) and the quenched CDs-AgNPs system is turned on after addition of Hg 2+ ions, which is due to higher affinity of Hg 2+ and AgNPs than that of CDs and AgNPs, thus resulting the disappearance of AgNPs from the CDs-AgNPs composites and leading to the fluorescence turn-on of CDs. The developed fluorescence turn-on approach exhibited high selectivity and sensitivity for detection of Hg 2+ . Under the optimum experimental conditions, good linearity was achieved over the range of 100–160 μM and the limit of detection (LOD) was estimated to be 2.22×10 -8 M for Hg 2+ . The recoveries of Hg 2+ spiked in real samples ranged from 98.4% to 101.6%. Results of this study suggest that the fluorescence turn-on approach can be used to the detection of Hg 2+ in real water samples.
研究领域:
分析化学
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环境科学
·
纳米技术
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DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2013
·Vol. 130
·期 2
·939-943
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IF 1.395
Lin Wang,Guangyou Zhang,Meishan Pei,Linlin Hu,Enyong Li,Huizhi Li
A lable-free, simple, and sensitive fluorescence “turn-on” approach is designed to rapidly detect protein using a conjugated polythiophene derivative (PDPMT-Cl). The fluorescence of PDPMT-Cl solution can be efficiently quenched by PtCl42− ions. Upon adding trypsin to the (bovine serum albumin, BSA) PDPMT-Cl–PtCl42− solution, the BSA is cleaved into amino acid or peptide fragments, which are stronger PtCl42− ions chelators to form more stable complexes with PtCl42− ions. Thus, the PtCl42− ion is displaced from PDPMT-Cl and the fluorescence of PDPMT-Cl is recovered. By triggering the “turn-on” signal of PDPMT-Cl, it is successful to detect the protein in real time. “Turn-on” response as readout signal is able to effectively reduce background noise and increase detection sensitivity. This method offers good selectivity for detecting protein in the presence of other common amino acids and metal ions. Under optimized conditions, the concentration of BSA in the range of 0.0004–1.75 mg/mL exhibits a linear relationship with the relative fluorescence intensity, and the correlation coefficient is 0.9997. The limit of detection is 4.47 × 10−4 mg/mL. The system is successfully applied for detecting protein in milk and egg. Due to the simplicity, sensitivity, and rapid response, this assay shows great potential for protein detection in the future. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 130: 939-943, 2013
研究领域:
分析化学
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生物化学
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高影响因子
RSC Advances
·
2022
·Vol. 12
·期 46
·30024-30029
·
IF 4.036
Baozhu Zhang,Ziyao Yang,Yuxia Li,Ling Ma,Fenfang Li,Xiuqing Lv,Guangming Wen
A label-free aptasensor has been fabricated in order to detect adenosine triphosphate (ATP) using turn-on fluorescence DNA-Ag NCs. The fluorescence of the DNA-Ag NCs could increase remarkably with the addition of ATP mainly because ATP specifically interacts with its aptamer to change the microenvironment of the darkish DNA-Ag NCs located at one terminus or two termini due to the conformational alteration of the aptamer structure. The proposed sensor can detect ATP in a linear range of 6–27 mM with a good detection limit of 5.0 μM. Additionally, the proposed method succeeded in detecting ATP in fetal bovine serum.
研究领域:
分析化学
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生物传感器
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纳米技术
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Yixia Yang,Behafarid Ghalandari,Liyun Lin,Xiao Sang,Wenqiong Su,Adeleh Divsalar,Xianting Ding
The abuse application of glyphosate can result in a potential hazard for environment and human, however its ultrasensitive detection remains challenging. Herein, a Cu 2+ modulated DNA-templated silver nanoclusters (DNA-AgNCs) sensor was constructed to sensitively determine glyphosate based on the turn-on fluorescence strategy. The fluorescence quenching of DNA-AgNCs occurred with the existence of Cu 2+ . Upon the presence of glyphosate, the functional groups on the surface of glyphosate could chelate with Cu 2+ , following the fluorescence recovery of DNA-AgNCs. Through the stoichiometric methods, we unveil that Cu 2+ -trigged fluorescence quenching mode is a combination of static and dynamic quenching with the static mode being predominant. In DNA-AgNCs/Cu 2+ system, the carboxylate, amine, and phosphonate groups of glyphosate interact with Cu 2+ through chelation, in which the carboxylate oxygen, the phosphonate oxygen atoms, and the monoprotonated secondary amine nitrogen atom and Cu 2+ form chelate rings. This fluorescence sensor showed a desired linearity of glyphosate analysis under the optimum conditions, ranging from 15 to 100 μg/L with a low detection down to 5 μg/L. Moreover, the proposed sensor was successfully utilized to measure glyphosate in real samples, indicating a promising application in pesticide residues detection.
研究领域:
分析化学
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环境科学
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纳米技术
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DOI
PubMed
高影响因子
ANALYST
·
2013
·Vol. 138
·期 17
·4737-4740
·
IF 3.969
Chenchen Ge,Junhua Chen,Wei Wu,Zhiyuan Fang,Lingbo Chen,Qi Liu,Lin Wang,Xuerong Xing,Lingwen Zeng
An enzyme-free and label-free fluorescence turn on biosensor for amplified copper(II) ion (Cu2+) detection has been constructed based on self-assembled DNA concatamers and Sybr Green I. This assay is simple, inexpensive and sensitive, enabling quantitative detection of as low as 12.8 pM Cu2+.
研究领域:
分析化学
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生物传感器
·
纳米技术
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Jian Zhu,Hui Chang,Jian-Jun Li,Xin Li,Jun-Wu Zhao
Both “turn-on” and “turn-off” fluorescent probe for the determination of melamine have been developed by tuning the inner filter effect (IFE) from CdTe quantum dots (QDs) to gold nanoparticles. Because of the melamine-induced aggregation of gold nanoparticles, the first absorption peak of the transverse surface plasmon resonance (SPR) at 520 nm fades down, whereas the second absorption peak of the longitudinal SPR around 620 nm gets intense. The decrease of the transverse SPR reduces the IFE of the bundled CdTe QDs with an emission band at 525 nm (the corresponding particle size is 1.98 nm), which results in the melamine concentration-dependent fluorescence recovery of CdTe QDs. The increase of the longitudinal SPR enhances the IFE of the bundled CdTe QDs with an emission band at 620 nm (the corresponding particle size is 3.68 nm), which results in the melamine concentration-dependent fluorescence decrease of CdTe QDs. The green mode detection with the fluorescence recovery has a linear response for melamine from 20 to 100 ng/mL with a theoretical detection limit of 8 ng/mL. The red mode detection with the fluorescence decrease has a linear response for melamine from 20 to 100 ng/mL with a theoretical detection limit of 11 ng/mL. Interference studies and real samples detection show that the melamine could be specifically detected using this dual-mode fluorescent probe.
研究领域:
分析化学
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纳米技术
·
环境科学
·
食品安全
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2015
·
IF 4.513
Yingfeng Qin,Yefei Ma,Xue Jin,Liangliang Zhang,Gaojie Ye,Shulin Zhao
As an important antitumor drug, bleomycin (BLM) is widely used in the treatment of a variety of cancers. In addition, nucleases play a crucial role in DNA replication, recombination and repair which are associated with cancer development. Thus, the development of BLM and nuclease detection methods is of great significance in cancer therapy and related biological mechanism research. Here, a WS 2 nanosheet-based turn-on fluorescent sensing platform for simple, fast and sensitive detection of BLM and nuclease was reported. WS 2 nanosheet exhibits different affinity toward ssDNA with different length and excellent fluorescence quenching ability. A fluorescein (FAM)-labeled long ssDNA could be adsorbed on the surface of WS 2 nanosheet and the fluorescence was therefore quenched. In the presence of BLM·Fe(II) or S1 nuclease (a ssDNA-specific nuclease which was used as a model enzyme), an irreversible scission of long ssDNA was underwent through the BLM-induced oxidation cleavage or S1 nuclease-induced enzymatic hydrolysis. Short FAM-linked oligonucleotide fragments which could not be adsorbed on the nanosheet surface were then produced, resulting in a weak fluorescence quenching after mixing WS 2 nanosheets. Thus, the fluorescence signal was restored. The proposed sensor displays a wide linear range and a high sensitivity with a detection limit of 0.3 nM for BLM and 0.01 U mL −1 for S1 nuclease. It also exhibits a good performance in complex biological samples. This method not only provides a strategy for BLM or S1 nuclease assay but also offers a potential application in biomedical and clinical study.
研究领域:
生物化学
·
分子生物学
·
分析化学
·
癌症研究
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）