DYES AND PIGMENTS
·
2018
·
IF 3.767
Zhuo Ye,Chao Xiong,Jian Pan,Dongdong Su,Lintao Zeng
A series of highly photostable, lysosome-targeted BODIPYs with tunable fluorescence from green to the near-infrared region was developed. The probes were found to show an accurate lysosome-targeting ability with high selectivity and high photostability under continuous excitation irradiation. The results show that these lysosome-targeted BODIPYs offer a simple and convenient tool for fluorescence imaging of lysosomes, which will be valuable for understanding the activity and function of lysosomes in related cells.
研究领域:
细胞生物学
·
生物化学
✓ 已验证
DOI
高影响因子
Nanoscale
·
2020
·Vol. 12
·期 24
·13010-13016
·
IF 6.895
Qiaoling Liu,Xiuyun Niu,Yan Zhang,Ying Zhao,Kaixin Xie,Boru Yang,Qing He,Shiyou Lv,Lin Li
The abnormal concentrations of both biothiols and pH in lysosomes are seriously related to many major diseases, such as Parkinson's and Alzheimer's diseases. Up to now, there are few reports that clearly illustrate the relationship between lysosomal pH and biothiols via fluorescence assay. Herein, novel carbon dots (Scy-CDs) are prepared with good water dispersibility and excellent photostability, and a large Stokes shift of 106 nm is exhibited under an excitation wavelength of 450 nm. The remarkable pH-dependent behavior of Scy-CDs is presented with the fluorescence quenching based on the donor-excited photoinduced electron transfer (d-PET) process. The pKa value is 5.30, which is in good agreement with the range of the normal and abnormal lysosomal pH. Upon the addition of cysteine (Cys) or homocysteine (Hcy), the d-PET process is effectively inhibited with fluorescence recovery totally. The significant co-localization of Scy-CDs with Lyso-Tracker Deep Red in HEp-2 cells and the Pearson correlation coefficient 0.88 strongly suggest that the Scy-CDs can target lysosomal pH and Cys/Hcy in living cells.
研究领域:
细胞生物学
·
医学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
Pharmaceutics
·
2024
·Vol. 16
·期 6
·744
·
IF 4.9
Qianqian Long,Xinmin Zhao,Lili Gao,Mengyuan Liu,Feng Pan,Xihui Gao,Changyou Zhan,Yang Chen,Jialei Wang,Jun Qian
Background:Nanoparticles conjugated with fluorescent probes have versatile applications, serving not only for targeted fluorescent imaging but also for evaluating the in vivo profiles of designed nanoparticles. However, the relationship between fluorophore density and nanoparticle behavior remains unexplored.Methods:The IR783-modified liposomes (IR783-sLip) were prepared through a modified ethanol injection and extrusion method. The cellular uptake efficiency of IR783-sLip was characterized by flow cytometry and fluorescence microscope imaging. The effects of IR783 density on liposomal in vivo behavior were investigated by pharmacokinetic studies, biodistribution studies, and in vivo imaging. The constitution of protein corona was analyzed by the Western blot assay.Results:Dense IR783 modification improved cellular uptake of liposomes in vitro but hindered their blood retention and tumor imaging performance in vivo. We found a correlation between IR783 density and protein corona absorption, particularly IgM, which significantly impacted the liposome performance. Meanwhile, we observed that increasing IR783 density did not consistently improve the effectiveness of tumor imaging.Conclusions:Increasing the density of modified IR783 on liposomes is not always beneficial for tumor near-infrared (NIR) imaging yield. It is not advisable to prematurely evaluate novel nanomaterials through fluorescence dye conjugation without carefully optimizing the density of the modifications.
研究领域:
药剂学
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生物医学工程
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分子影像学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
·
2020
·Vol. 146
·期 4
·875-882
·
IF 3.656
Wang Maonan,Wang Lishan,Feng Huan,Jiang Hui,Zhou Jiahua,Wang Xuemei
Purpose Presently, liver cancer is still one of the malignant tumors with high mortality. As far as the treatment of liver cancer is concerned, the most effective method is still liver transplantation. But every year, many liver cancer patients die from the lack of a proper liver transplant, or from waiting for a liver transplant. Therefore, it is very important to find new and effective treatment for patients with liver cancer. Methods Herein, the cell model and the orthotropic liver tumor mice model have been performed to verify the results of our treatment. We found that the in situ synthesized gold nanocluster–PTEN (GNC–PTEN) complexes can effectively target and realize the fluorescence imaging of the liver tumor. Results GNC–PTEN complexes could inhibit the proliferation, invasion, and metastasis of liver cancer cells. And the results also showed that GNC–PTEN complexes could be well targeted liver tumor at 6 h and the liver tumor in mice group treated with GNC–PTEN complexes almost disappeared. Conclusion This is a simply and effectively method to realize liver cancer imaging and inhibition. This may raise the possibility for the accurate image/diagnosis and simultaneously efficient treatment of liver cancer in the relevant clinic application.
研究领域:
肿瘤学
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分子生物学
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纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nanoscale
·
2019
·Vol. 11
·期 17
·8458-8463
·
IF 6.97
Haifang Liu,Yuanqiang Sun,Zhaohui Li,Jie Yang,Aaron Albert Aryee,Lingbo Qu,Dan Du,Yuehe Lin
Formaldehyde (FA) is involved in many biological processes and is closely connected with many diseases including Alzheimer's disease and cancer. Therefore, methods for sensitive and selective detection of FA in living cells are highly demanded. As a new class of carbon nanomaterials, carbon dots (CDs) have attracted great attention owing to their robust photostability, good biocompatibility and environmental friendliness. In this manuscript, the first lysosome-targeted CDs for ratiometric fluorescence detection of FA were efficiently prepared from dexamethasone and 1,2,4,5-tetraaminobenzene through the microwave-assisted hydrothermal method. These CDs show highly selective and sensitive sensing ability towards FA with fast response and great changes of ratio values. The CDs exhibit robust photostability and good biocompatibility and were successfully employed in ratiometric fluorescence bioimaging of FA fluctuations in lysosomes of living cells, which demonstrates their great practicability in FA-related bioanalysis and biological studies.
研究领域:
生物化学
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纳米医学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Le Liang,Su-qian Cai,Yan-li Leng,Chan Huang,Yong-qing Liu,Ye Wang,Li Luo,Mei Han,Xiao-hong Li,Xiao-hua Cai
Ciprofloxacin (CIP) and gallium (Ga) are widely used in many fields and their excessive presences will pose serious threats to the environment and life health. Therefore, developing an intelligent detection method for rapidly tracking and determination of CIP and Ga 3 ⁺ concentration in environments and organisms is of great significance. In this work, a europium-doped carbon dots (Eu-CDs) with unique structure and performances was prepared by a one-pot hydrothermal method. Eu-CDs can efficiently overcome the problem of aggregation-induced fluorescence quenching in complex environments to track CIP and Ga 3⁺ with highly sensitivity. The "antenna effect" of non-radiative energy transfer is introduced to explain the pathway on fluorescence transition of Eu-CDs through DFT theoretical calculations and mechanism investigations, which provide a theoretical basis for the design and development of novel carbon dots with specific functions. Eu-CDs can successfully be applied to fluorescence imaging in organisms, moreover, it also exhibits excellent application potentials in the field of anti-tumor and antibacterial. In addition, a portable intelligent detection platform based on Eu-CDs-hydrogel device have been developed, which provides a new strategy for rapidly detection of CIP and Ga 3⁺ in real samples.
研究领域:
分析化学
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环境科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2024
·
IF 3.7
Shiqiang Hou,Ning Lin,Yi Wang
Red-emitting fluorescent carbon dots (CDs) have garnered significant attention due to their wide-ranging applications in biological fields. However, challenges such as complex precursors, labor-intensive preparation processes, and low quantum yields have hindered their broader utilization. In this study, we developed a simple and efficient solvothermal method to synthesize fluorescent CDs with tunable emission wavelengths using aniline derivatives as precursors. The emission wavelengths of the synthesized CDs were influenced by the functional groups at the para-position of the aniline derivatives with stronger electron-donating effects leading to a red shift in emissions. Notably, bright red-emitting CDs with a quantum yield of 19.42% and excellent photobleaching resistance were obtained by using p-phenylenediamine as the sole precursor. These CDs exhibited sensitivity to Fe3+ ions, demonstrating a strong linear detection range (R2 = 0.999) from 0 to 50 μM. Additionally, the CDs were uniform in size (2–5 nm), emitted stable red fluorescence in pH conditions ranging from 4 to 10, and were successfully internalized by glioma cells, enabling precise fluorescence imaging of gliomas both in vitro and in vivo.
研究领域:
生物医学工程
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材料科学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2021
·Vol. 9
·期 22
·4577-4586
·
IF 6.331
Yongjia Tang,Haoran Xu,Yaxue Dai,Fang Wang,Wenjing Huang,Peifei Liu,Yueqing Gu
Hepatocellular carcinoma (HCC) is one of the most common malignant tumors with limited diagnosis. Mesenchymal epithelial transition factor (c-Met) has become a hot target for cancer diagnosis and therapy, which is overexpressed in HCC. In this study, we labeled a novel c-Met targeting peptide YQ-M3 with a near-infrared fluorescent dye MPA and a radionuclide technetium-99m for HCC detection. YQ-M3-MPA showed high affinity for c-Met positive HepG2 tumor in vitro and higher tumor uptake and higher T/N ratio than GE137-MPA (a positive tracer for c-Met) in HepG2 tumor-bearing mice in vivo by fluorescence imaging. In addition, 99mTc-HYNIC-YQ-M3 also showed significant tumor uptake in vivo through SPECT imaging. These results indicated that c-Met positive tumors were successfully detected via fluorescence and SPECT imaging using YQ-M3-MPA and 99mTc-HYNIC-YQ-M3, respectively, and further suggested that YQ-M3-MPA and 99mTc-HYNIC-YQ-M3 have some possibly potential clinical applications for HCC diagnosis.
研究领域:
肿瘤学
·
分子生物学
·
医学成像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2013
·
IF 7.604
Ke Ding,Lihong Jing,Chunyan Liu,Yi Hou,Mingyuan Gao
Magnetically engineered Cd-free CuInS 2 @ZnS:Mn quantum dots (QDs) were designed, synthesized, and evaluated as potential dual-modality probes for fluorescence and magnetic resonance imaging (MRI) of tumors in vivo . The synthesis of Mn-doped core–shell structured CuInS 2 @ZnS mainly comprised three steps, i.e., the preparation of fluorescent CuInS 2 seeds, the particle surface coating of ZnS, and the Mn-doping of the ZnS shells. Systematic spectroscopy studies were carried out to illustrate the impacts of ZnS coating and the following Mn-doping on the optical properties of the QDs. In combination with conventional fluorescence, fluorescence excitation, and time-resolved fluorescence measurements, the structure of CuInS 2 @ZnS:Mn QDs prepared under optimized conditions presented a Zn gradient CuInS 2 core and a ZnS outer shell, while Mn ions were mainly located in the ZnS shell, which well balanced the optical and magnetic properties of the resultant QDs. For the following in vivo imaging experiments, the hydrophobic CuInS 2 @ZnS:Mn QDs were transferred into water upon ligand exchange reactions by replacing the 1-dodecanethiol ligand with dihydrolipoic acid-poly(ethylene glycol) (DHLA-PEG) ligand. The MTT assays based on HeLa cells were carried out to evaluate the cytotoxicity of the current Cd-free CuInS 2 @ZnS:Mn QDs for comparing with that of water soluble CdTe QDs. Further in vivo fluorescence and MR imaging experiments suggested that the PEGylated CuInS 2 @ZnS:Mn QDs could well target both subcutaneous and intraperitoneal tumors in vivo .
研究领域:
生物医学工程
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材料科学
·
影像学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2020
·Vol. 8
·期 35
·8002-8009
·
IF 5.344
Yuman Dong,Pengcheng Du,Peng Liu
Carbon dots (CDs) have attracted intense attention in tumor nanotheranostics recently; however, those nanotheranostics exhibited similar fluorescence in both normal and tumor tissues, limiting their practical application. In the present work, absolutely “off–on” fluorescent CD-based nanotheranostics was designed for tumor intracellular real-time imaging and pH-triggered DOX delivery via both static quenching by the crosslinking of benzaldehyde-containing diblock copolymers and dynamic quenching because of the surrounding conjugated DOX molecules. The proposed PPEGMA42-b-PFPMA122–(CDs)–DOX nanotheranostics did not exhibit fluorescence in a normal physiological medium, while strong fluorescence recovery occurred in the tumor intracellular microenvironment due to pH-triggered disintegration, releasing the CDs and DOX. The pH-triggered DOX release and absolute “off–on” fluorescence make the proposed nanotheranostics promising for tumor-specific pH-triggered DOX delivery and imaging.
研究领域:
生物医学工程
·
纳米医学
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Dan Zhao,Xiaoyun Li,Mengyu Xu,Yan Jiao,Huan Liu,Xincai Xiao,Haiyan Zhao
The preparation of functional long-wavelength-emitting nanomaterials and the researches on their applications in antibacterial and antibiofilm fields have important significance. This paper reports the preparation of yellow-green-fluorescent and high- quantum yield carbon dots (4-ACDs) with 4-aminosalicylic acid and polyethylene imine as raw materials through one-step route, and the impacts of raw material structure and the reaction conditions upon the optical properties of the products have been investigated. 4-ACDs exhibit excellent broad-spectrum antibacterial activity , and their good biocompatibility ensures them as ideal fluorescent nano-probe for cell imaging . However, 4-ACDs could not effectively eliminate the biofilm of Staphylococcus aureus ( S. aureus ). CDs-LZM complex was prepared through the coupling between 4-ACDs and lysozyme (LZM) and the complex showed strong antibacterial activity against Gram-positive bacteria, particularly with MIC against S. aureus at 5 μg mL −1 . Besides, CDs-LZM showed excellent ability against the biofilm of S. aureus . At the concentration of 60 μg mL −1 , its inhibition rate against the growth of biofilm was 86 %, and elimination rate against biofilm reached 76 %. CDs-LZM exhibited obvious antibiofilm ability through removing extracellular matrix of biofilm, greatly reducing the thickness of biofilm under confocal microscopy . The application of novel long-wavelength-emitting nanomaterial in eliminating pathogenic bacteria is of great significance.
研究领域:
生物医学
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纳米材料科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Laser & Photonics Reviews
·
2024
·Vol. 18
·期 12
·2400786
·
IF 9.8
Wang Wang,Jinqiu Yang,Bolin Ma,Haoyu Zhu,Yaqi Zhu,Yuexi Yi,Xiaodan Li,Qiqing Li,Haoran Chen,Bin Li,Xianggui Kong,Yulei Chang
Fluorescence/luminescence bioimaging, utilizing rare-earth doped nanocrystals (RENCs) within the NIR-IIb (1500–1700 nm) sub-window, has drawn interest for its deep penetration depth and superior resolution. Herein, Er 3+ /Yb 3+ couple is rationally designed in topologically arranged RENCs that facilitate a boosted 1530 nm emission of Er 3+ via premeditated Yb 3+ -mediated energy transfer processes under 808 nm excitation. To maximize this modulation effect for boosting 1530 nm emission, a regional active ions concentration and spatial regulation strategy is explored in the optimized concentration and core–shell–shell structure (NaErF 4 :20%Yb@NaYbF 4 :20%Er@NaYF 4 with a quantum yield of 16.46% in NIR-IIb region). Leveraging the superior emission and multi-wavelength excitation characteristics of the as-obtained nanocrystals, a NIR-IIb-based ratiometric fluorescence imaging measurement is achieved under 808 and 980 nm excitation, which cannot only show the 2D distribution of probes but also provide spatial depth information (range from 0 to 2.8 mm with a tissue depth resolution of 66 µm). The findings hold significant potential for advancing both vascular science and clinical medicine.
研究领域:
生物医学工程
·
材料科学
·
光学成像
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）