MICROCHEMICAL JOURNAL
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2020
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IF 3.594
Lu Xu,Qiong Shi,Daowang Lu,Liuna Wei,Hai-Yan Fu,Yuanbin She,Shunping Xie
The feasibility of simultaneous detection of multiple cheap materials in kiwifruit juice (KFJ) was studied by two fluorescent methods and chemometrics. Because the actual frauds usually involve many known and unknown adulterants, the traditional methods to detect one or more known components are insufficient to identify adulterated KFJ. Therefore, class models of pure KFJ were established using one-class partial least squares (OCPLS) to perform untargeted analysis. To enhance the detection specificity, traditional and double-quantum-dots enhanced fluorescent spectroscopic techniques were combined to characterize pure and adulterated KFJ. By data fusion and using standard normal variables (SNV) transformation, OCPLS model with sensitivity of 0.929 was obtained. The proposed method could detect adulterations of 2% (w/w) or more syrup and artificial fruit powder. The fusion of traditional and quantum dots enhanced fluorescence was demonstrated to provide a rapid and highly sensitive method for untargeted analysis of adulterated KFJ.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Sensors & Diagnostics
·
2022
·Vol. 2
·期 1
·168-175
·
IF 0
Lingfeng Xu,Yuefeng Wang,Chuan Jiang,Yanrong Huang,Genhe He,Limin Liu
Liquid safety has become one of the urgent affairs in global public health studies, and a noninvasive and effective viscosity inspection method is expected to ease the burden of continuously increasing health problems. In this study, one kind of activatable molecular sensor (DTPMP) based on a salicylaldehyde derivate has been synthesized via the Schiff base condensation reaction. This sensor is comprised of a donor–acceptor conjugated structure. We proposed the viscosity of the liquid microenvironment as a marker, and a rotatable conjugate structure was utilized as the recognition site. The molecular sensor was synthesized in a one-step facile way, and DTPMP displayed a longer emission wavelength and larger Stokes shift (142.5 nm in water, 116.8 nm in glycerol), which endows the sensor with the capacity of avoiding auto-fluorescence and achieving high signal-to-noise ratio imaging. The rotation of aromatic rings in DTPMP is restricted in a high-viscosity microenvironment, with the gradually increasing fluorescence emission signal at 532 nm. Moreover, the DTPMP sensor exhibits high adaptability, selectivity, sensitivity, and good photostability in various commercial liquids. Significantly, this sensor has successfully been applied not only in the determination of the thickening effects of food gum but also in the detection of viscosity enhancement during the liquid food spoilage process, which may be helpful in screening the metamorphic stage. We expected that this unique strategy will reinvigorate the continued perfection of the fluorescence technique toward liquid safety investigation.
研究领域:
分析化学
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生物医学工程
·
公共健康
✓ 已验证
DOI
Chemosensors
·
2025
·Vol. 13
·期 3
·107
·
IF 3.7
Haiyan Chang,Hailong Wu,Tong Wang,Xiaozhi Wang,Ruqin Yu
In this study, an excitation–emission–pH multi-way fluorescence technique coupled with a third-order calibration method based on an alternating quadrilinear decomposition (AQLD) algorithm was proposed for the simultaneous determination of thiabendazole (TBZ) and carbaryl (CAR) in apples. AQLD can be considered a “mathematical separation” technique that extracts the pure signal of the target analyte from complex mixed signals, thereby effectively addressing fluorescence peak overlap and unknown interference. The average spiked recoveries of the target analytes ranged from 98.4% to 101.9%, and the relative standard deviation was less than 5.6%. To evaluate the performance of the method, a number of parameters were calculated, including sensitivity (SEN), selectivity (SEL), limit of detection (LOD), limit of quantification (LOQ), and intra-day and inter-day precision. The results of the third-order calibration method were compared with those of the second-order calibration method (based on excitation–emission matrix fluorescence). These results showed that the former was superior. In short, the proposed strategy is simple, cost-effective, and anti-interference, providing a valuable reference for accurate quantification of TBZ and CAR in complex food matrices with uncalibrated interferences.
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2016
·
IF 2.653
Yunchang Fan,Xing Dong,Xiaojing Li,Yingying Zhong,Jichuan Kong,Shaofeng Hua,Juan Miao,Yan Li
The effects of ionic liquids (ILs) on the lipase activity were studied by UV–Vis spectroscopy and the IL-lipase interaction mechanism at the molecular level was investigated by fluorescence technique. Experimental results indicated that the lipase activity was inhibited by ILs and the degree of inhibition highly depended on the chemical structures of ILs. The inhibitory ability of the Cl − - and Br − -based ILs increased with increasing the alkyl chain length in the IL cation. Thermodynamic parameters, enthalpy change (Δ H ) and entropy change (Δ S ) were obtained by analyzing the fluorescence behavior of lipase with the addition of ILs. Both Δ H and Δ S were positive suggesting hydrophobicity was the major driven force for the Cl − - and Br − -based ILs. For the BF 4 − -, CF 3 SO 3 − -, ClO 4 − - and N(CN) 2 − -based ILs, hydrogen bonding was the main driven force. For a more comprehensive understanding of the effects of ILs on lipase activity, the roles of hydrophobicity and hydrogen bonding must be considered simultaneously. A regression-based equation was developed to describe the relationship of the inhibitory ability of ILs and their hydrophobicity and hydrogen bonding ability.
研究领域:
生物化学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Environmental Technology & Innovation
·
2024
·
IF 6.7
Di Ju,Chao Hu,Yuntong Lv,Yuting Li,Ping Gao,Xueqing Yang
The insecticide resistance development poses challenges to the pest control effectiveness. As phase III detoxification enzyme, ATP-binding cassette (ABC) transporters play a crucial role in conferring resistance by facilitating the elimination of insecticides. However, the precise involvement of ABCs in insecticides metabolism remains unclear. In this study, a competitive assay utilizing microinjection of the ABC substrate Texas Red (TR) was established in Lepidoptera for the first time to investigate the contribution of ABCs to the detoxification process of Cydia pomonella towards lambda -cyhalothrin (LCT) and abamectin (ABM). Results revealed that the elimination of TR was hindered by ABC inhibitor verapamil, LCT, or a combination of both, implying a shared subsets of ABCs involved in the transportation of TR and LCT. Moreover, the application of ABM alone led to marked inhibition of TR elimination, with verapamil not further exacerbating this inhibitory effect, indicating that ABM exhibited a highly competitive ABC substrate characteristic and has a strong interaction with them. Furthermore, silencing key resistance associated ABC genes through RNAi resulted in increased mortality of larvae under LCT or ABM exposure, affecting TR elimination and ATP hydrolysis. These findings imply that ABC genes are critical for the active transport function of ABCs energized by ATP, ultimately reducing larvae tolerance to insecticides. In conclusion, our findings not only validated the feasibility of a competitive bioassay using fluorescent ABC substrate in lepidopteran larvae but also highlighted that ABCs in enhancing the efflux capacity of C. pomonella larvae, facilitating the binding and transportation of insecticides and their metabolites.
研究领域:
昆虫学
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分子生物学
·
毒理学
✓ 已验证
DOI
高影响因子
CORROSION SCIENCE
·
2024
·
IF 7.4
Xin Guo,Xiaolun Ding,Yaxin Wang,Jinke Wang,Weimin Tan,Yiran Li,Zhibin Chen,Zongbao Li,Weiting Chen,Lingwei Ma,Dawei Zhang
A high-throughput screening method is developed for rapid evaluation of corrosion inhibitors. By employing a fluorometric technique, this method quickly assesses the concentration of iron ions converted from corrosion products in the reaction pool to rapidly evaluate the corrosion inhibition performance of amino acid and surfactants. Utilizing this high-throughput screening platform, the corrosion inhibition effects of L-cysteine mixed with quaternary ammonium salts of different alkyl chain length were evaluated, and their optimal mixing ratios were determined. Through corrosion experiments and molecular simulations, the synergistic mechanisms of the inhibitor mixtures are revealed.
研究领域:
材料科学
·
化学工程
·
腐蚀科学
✓ 已验证
产品关联
DOI
高影响因子
CONSTRUCTION AND BUILDING MATERIALS
·
2019
·
IF 4.046
Lei Cao,Chun Liu,Hongyan Tian,Dandan Jia,Dongjun Wang,Yang Xu,Jintang Guo
The mechanism of synthetic polymer fluid loss additive (FLA) was based on adsorption of FLA on the surface of hydrating cement particles and the formation of a complex network structure, resulting in decline of cement slurry filtrate. However, due to the complexity of early hydration environment of cement slurry, there was lack of effective and intuitive way to characterize the adsorption interaction between FLA and cement particles. In this study we described our quest in furthering understanding of the adsorption action of fluid loss additive on the cement minerals. By fluorescence staining technique (combined polymer additives with organic fluorescent dyes), we observed that the skin of the hydrated particles was coated with dyed polymer, which led to a successful fluid loss additive. More importantly, the analysis of the fluorescence intensity of luminous polymer in cement pore solution distinguished the respective adsorption capacity of various admixtures (dyed and undyed) on the surface of the cement particles, so as to solve the problem of the traditional method in characterizing the competitive adsorption between polymers, thus gave a convenient way to analyze the interaction mechanism between polymer admixture and cement particles from the theory and technology.
研究领域:
材料科学
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化学工程
·
土木工程
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Zaifa Pan,Di Yang,Jing Lin,Kang Shao,Shuxian Shi,Yuan-Jie Teng,Huijun Liu,Yuanbin She
Carcinoembryonic antigen (CEA) is an important biomarker in the diagnosis of cancer. The increase of CEA in malignant pleural effusion appears earlier and possesses higher clinical diagnostic value than that in the serum. Conventional fluorescent probes suffer from the interference of strong biotissue auto-fluorescence, which limits severely their applications in biology detection. Herein, a novel fluorescence aptasensor was designed with near-infrared persistent luminescence nanoparticles (PLNPs) for accurate detection of carcinoembryonic antigen in pleural effusion by FRET quenching and recovery mechanism. The strong background interference from the autofluorescence of pleural effusion samples can be effectively eliminated and extra increments of measured values originated from the background of different samples were ruled out, benefit from the long decay time of PLNPs and time-resolved fluorescence technology . The detection results show high accuracy of the measured values of carcinoembryonic antigen both in cancer and benign disease group with low detection limit up to 0.0851 pg mL −1 . Furthermore, excellent selectivity from coexisting biomarker was achieved by the hybridization between the aptamer and the complementary DNA on PLNPs surface. Hence, the established near-infrared PLNPs-based aptasensors offer excellent performance with high selective, accuracy and signal-to-noise ratio for detection of carcinoembryonic antigen in pleural effusion.
研究领域:
癌症诊断
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生物医学工程
·
纳米技术
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Xiqing Liu,Tao Wang,Yongqing Wang
Fluorescence technology enabled highly sensitive, real-time and multi-scenario detection of lysozyme (Lys). However, fluorescent nanomaterials suffer from aggregation-induced quenching, environmental interference and lack of selective recognition capability. In this work, the ZIF-8 was used as a framework to support red and blue fluorescence quantum dots for constructing ratiometric fluorescence sensor. At the same time, combined with molecular imprinting technology, a Molecular Imprinting Polymers (MIPs)-based ratiometric fluorescence sensor was designed for improving the sensitive and selective. The MIPs-based ratiometric fluorescence sensor exhibited highly sensitively and selectively for the detection of lysozyme. Benefited from ratiometric design, the sensor could realize the effect of visual detection of Lys and the limit of detection (LOD) was reached 0.048 mg/L. The HPLC method was used to further confirm the sensor has the potential for practical application. Furthermore, to the demand of an affordable and portable strategy for visual detection Lys, a dexterous and inexpensive test paper was made in this work.
研究领域:
分析化学
·
纳米技术
·
荧光传感
·
材料科学
·
生物传感
·
环境监测
✓ 已验证
产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2025
·
IF 5.6
Zhuo Xiong,Qingfeng He,Yong Zeng,Jimin Chen
To address the issue that traditional glass processing technologies struggle to achieve complex structural forming and precise doping of functional elements, this study employed vat photopolymerization (VPP) additive manufacturing technology to fabricate quartz glass with high-quality mechanical and optical properties. Moreover, doping was performed on the glass slurry, enabling the conversion of quartz glass into ultraviolet-excited luminescent glass. A comprehensive investigation was conducted on process optimization, microstructural characteristics, mechanical properties, and fluorescence performance. By optimizing the rheological properties of the photocurable slurry containing 58 wt% SiO 2 and the vacuum sintering process (sintered at 1240°C), highly dense amorphous glass was successfully prepared, which exhibited a light transmittance of >80%, a Vickers hardness of 758.2 HV, and a relative density of >99.9%. A specific doping method was designed to achieve the controllable doping of Ce 3+ ions. Under natural light, the doped and undoped regions are indistinguishable. Using this technology, luminescent glass with controllable doped regions was fabricated. This technology demonstrates the capability of photoluminescent site-selective doping and exhibits differentiated optical responses under visible light and ultraviolet light, thereby providing a customizable solution for next-generation information encryption materials.
研究领域:
材料科学
·
增材制造
·
光学材料
·
荧光技术
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信息安全
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）