CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yijun Yao,Jiannan Xue,Miao Wang,Dong Fu,Yanqin Shen,Ying Xue,Fan Zhang,Pengbi Liu,Hongru Wang,Hailiang Wu
Herein, a facile, green, and universal method to prepare polymer fluorescent films was proposed. Firstly, hollow cellulose-based microspheres were prepared by cross-linking copolymerization and self-assembly strategy. Then, after encapsulating the hydrophobic fluorescent dye fluorescein isothiocyanate (FITC) into the cavity of a hollow cellulose-based microsphere through the ultrasonic/dialysis method, a series of flexible fluorescent microsphere films with tunable fluorescence was obtained. The formation of hollow cellulose-based microspheres was confirmed by SEM. DLS, AFM, and UV–vis analysis provided evidences for the successful encapsulation of FITC in hollow cellulose-based microspheres. Benefiting from the synergistic effects of “anchoring”, “diluting” and “isolation” for cellulose polymer chain as well as the hollow structure, the aggregation and self-quenching of FITC were effectively inhibited, which promoted the fluorescent films with different loading amount to emit green fluorescence under 365 nm UV light irradiation, and the fluorescence intensity was tunable. Moreover, the fluorescent films exhibited strong UV absorption, excellent optical stability, foldability, and easy scrollability. Particularly, compared with the luminescent film constructed by whisker-like cellulose-based microgel, the hollow cellulose-based fluorescent microsphere film obtained in this work had higher solvent resistance, water contact angle, and thermal stability. Due to the high stability and easy processability of hollow cellulose-based fluorescent microspheres, they could be processed into coating materials and fibers that were durable and widely used. They have potential application value in the fields of light-emitting devices, safety warnings, smart labels, and anti-counterfeiting materials, etc.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Yayue Mei,Xiaoyan Pan,Jianzhang Pan,Mengting Zhang,Hong Shen
MicroRNAs (miRNAs) have emerged as essential biomarkers for disease diagnosis, and several techniques are available to determine type 2 diabetes (T2D) relevant miRNAs. However, detecting circulating miRNAs can be challenging due to their small size, low abundance, and high sequence similarity, often requiring sensitive detection approaches combined with additional amplification processes. Laser-induced fluorescence (LIF) is a classic analytical method suitable for sensitively detecting trace amounts of nucleotide acid. Duplex-specific nuclease (DSN)-mediated amplification recently gained attention due to its catalytic activity based on target recycling, demonstrating a promising approach for miRNA amplification. This work developed a novel N -annulated perylene fluorescent dye to create a biosensor to analyze the miRNA (miR-223) relevant to T2D. The amine-reactive fluorescent dye assists the amidation reaction for nucleotide labeling, giving the oligonucleotide probe a high fluorescence quantum yield and sufficient water solubility. By combining the locked nucleic acid (LNA) modified oligonucleotide fluorescent probe to enhance the stability of LNA/RNA hybrids, thereby improving the DSN-mediated target miR-223 recycling for signal amplification, the proposed biosensor can highly selectively determine miR-223 with a limit of detection (LOD, S/N = 3) of 9.5 pM. When applied to real-world samples, the biosensor demonstrated its potential to distinguish between T2D patients and healthy controls.
研究领域:
生物化学
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医学诊断
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Tuanwei Li,Le Liu,Titao Jing,Zheng Ruan,Pan Yuan,Lifeng Yan
Photobleaching and biotoxicity are the main bottlenecks for organic fluorescent dyes applied in real-time dynamic monitoring of living cells. Here, an unnatural amino acid, 4-nitro-3-phenyl-l-alanine (NPA), was used as a scaffold to covalently link a near-infrared fluorophore Cy5.5 and an amphiphilic polypeptide, poly[oligo(ethylene glycol) methyl ether methacrylate]-block-poly[2-amino-N4-(2-diisopropylamino-ethyl)-l-aspartic acid] (P(OEGMA)21-P(Asp)16-iPr), was then conjugated for increasing the photostability and improving the biocompatibility simultaneously. The protective agent of NPA can service as an effective triplet state quenching by intramolecular electron transfer between Cy5.5 and NPA. The less sensitivity of the electron-transfer process for molecular oxygen makes it an ideal photostabilized strategy for fluorophores applied in live-cell imaging. Bonding to copolymer is a common way for hydrophobic dyes to expand their application in biomedical imaging and increase their functionality, depending on the delivery system. The results indicate that Cy5.5–NPA-linked polypeptide copolymer exhibited an enhanced photostability and an excellent biocompatibility, which means this scaffolding strategy has a potential application in fluorescence-guided surgery, lived-cell imaging, and super-resolution microscopy.
研究领域:
生物化学
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材料科学
·
细胞生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2021
·Vol. 139
·期 6
·51625
·
IF 3.125
Yingshi Zhao,Qinyong Song,Yuebin Lin,Feng Chu,Yanchun Wei,Sen Liu,Changjiang Pan,Li Quan,Yiqing Wang
Fluorescein is a traditional organic fluorescent dye that has the disadvantage of agglomeration-induced fluorescence quenching and photobleaching due to its large hydrophobic conjugate π-π system, which makes them have limitations in many fields of application. A method of polymer modification is revealed to address this issue. In this study, fluorescein molecules were modified by polycaprolactone (PCL) to obtain PCL grafting fluorescein (P x F), and subsequently assembled with PEG-PCL to obtain fluorescent nanoparticles (PPxF). PPxF would realize long term cellular fluorescence imaging. PCL polymerization induces self-assembly, which effectively avoids fluorescence quenching caused by fluorescent molecules aggregation, and at the same time well inhibit the energy transfer with oxygen to improve photostability. This method is a good way to improve the fluorescence properties of fluorescent dyes, to be suitable for all traditional organic fluorescent dyes to greatly improve their fluorescence and hydrophilic properties and enhance their application fields.
研究领域:
生物材料学
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分子生物学
·
化学工程
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Lulu Lv,Junhao Qu,Zihua Yu,Daihuan Chen,Chunxia Zhou,Pengzhi Hong,Shengli Sun,Chengyong Li
Microplastics (particle size <5 mm) are an emerging contaminant for aquatic environmental, which have attracted increasing attention in worldwide range. In this study, an improved fluorescent staining method for detection and quantification of microplastics was developed based on thermal expansion and contraction. This method is effective in detection of polyethylene, polystyrene , polyvinyl chloride and polyethylene terephthalate plastic particles. In order to avoid error statistics caused by pretreatment , various characterizations of microplastics were measured after heated, such as microstructure, compositions and thermostability. The results showed that there was no significant damage to microplastics even under heating condition at 75 °C for 30 min, and the stained microplastics had strong stability for up to two months. Moreover, this method has been successfully applied to the quantification of microplastics in biological samples and result showed there were about 54 particles g −1 (dry weight) microplastics in the Sipunculus nudus. This new method provides a reliable method for quantitative analysis of microplastics in environment and biological tissue.
研究领域:
环境科学
·
生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF LUMINESCENCE
·
2024
·
IF 3.3
Yining Zhang,Bingrong Shi,Baiyu Zhang,Hao Lv,Shuaiyi Zhang,Maorong Wang,Xia Wang
This study presents a novel approach in coherent dye random laser (RL) generation by utilizing the special properties of Enteromorpha prolifera (EP) within a micro-glass tube configuration. The methodological simplicity of injecting a mixed solution of dye and EP into the tube at room temperature achieves a novel way of realizing the RL system, characterized by tunable random lasing thresholds contingent upon the concentration of EP. This tunability stems from the synergistic interplay between photon enhancement and fluorescent dye absorption facilitated by the unique nanoporous structure of EP, culminating in the attainment of a low lasing threshold of 21.7 μJ/mm 2 at an optimized EP concentration of 2 mg/mL. With an excess EP concentration, the nanoporous structure could absorb large amounts of dye, resulting in dye fluorescence reabsorption, and causing the threshold to increase. A power Fourier transition was performed to calculate the effective optical cavity of the RL system, depicting the process of the coherent RL generation. Furthermore, a comprehensive investigation into the emission stability of the RL system revealed remarkable consistency in emission intensity over a prolonged excitation duration of 20 min, underscoring the potential for integration with optoelectronic devices . The L-shape mask imaging of the RL provides high-quality images with low spatial coherence, paving a useful application for speckle-free imaging. These results offer an affordable approach to achieving high-quality and low-threshold RLs using novel biomaterials.
研究领域:
光学
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生物材料科学
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产品关联
DOI
高影响因子
SEED SCIENCE AND TECHNOLOGY
·
2012
·Vol. 40
·期 3
·396-403
·
IF 0.62
Tian Y.X.,Guan Y.J.,Hu J.,Wang Q.T.,Hu Q.J.,Zhu S.J.
Five fluorescent dyes – rhodamine B (RB), rhodamine 6G (R6G), lissamine rhodamine B (LRB), 3, 6-diamincacridine hulmisulfate (DH) and safranine T (ST) – were used to label seeds as an anti-counterfeiting technology. The effects of the d yes on physiological parameters of
rape and the patterns of fluorescence of rape seedlings were investigated. The results showed that s oaking seeds for 24 hours in 0.1 mg RB ml−1, 0.1 mg LRB ml−1 or 0.1 mg ST ml−1 had no significant effects on seed germination, seedling growth,
seedling protective enzyme activities or chlorophyll content. RB and LRB emitted bright red and bright orange fluorescence under a fluorescence microscope, when excited by green light (546 nm) and blue light (495 nm), respectively, whereas ST showed little difference compared with the control.
Moreover, the vascular bundles of stem, seedling roots and aerial parts of seedlings treated with RB all emitted brilliant fluorescence for a long time, which could be used as a marker in seedlings. Our results suggest that labelling rape seeds with RB dye at a suitable concentration can be
used as an anti-counterfeiting technique.
研究领域:
植物学
·
农业科学
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry C
·
2020
·Vol. 8
·期 35
·12308-12313
·
IF 7.059
Ying Tang,Wenqian Cao,Lijia Yao,Yuanjing Cui,Yang Yu,Guodong Qian
Encapsulating luminescent dyes in metal–organic frameworks (MOFs) for white-light illumination and displays has received tremendous attention in recent years due to the unique colour-tuning flexibility and designability. However, the luminescent dyes may suffer from degradation due to moisture and oxygen under operating conditions. The stability and reliability of dye-encapsulated MOF phosphors remain challenges to be addressed. Herein, a broadband-emitting MOF phosphor is prepared via an ambient self-assembly process with pyrromethene 546 (pm546), pyrromethene 605 (pm605) and sulforhodamine 101 (SRh101) simultaneously confined in porous nanoscale zeolitic imidazolate framework-8 (ZIF-8). The photostability and thermostability of the pristine phosphor are evaluated in detail. By further coating it with thermoplastic polyurethane (TPU), the relative fluorescence intensity of the pristine phosphor is remarkably enhanced by 52.54%. A highly-stable remote-type incandescent white-lighting device is fabricated with a 460 nm blue LED chip with CIE chromaticity coordinates of (0.465, 0.413), a correlated colour temperature (CCT) of 2642 K and a colour rendering index (CRI) of up to 85.
研究领域:
材料科学
·
光学工程
✓ 已验证
DOI
高影响因子
CHEMICAL PHYSICS
·
2018
·
IF 1.707
Jingni Fu,Luning Zhang
The protonation/deprotonation equilibrium of a fluorescent pH probe (carboxy-seminaphthorhodafluor-1, SNARF-1) within the nanoscale water layer confined in common black films (CBFs) has been studied. We find that SNARF-1 molecules feel a more acidic environment in CBFs than when they are in the bulk micellar solution, using the base/acid peak area ratio of the dye to indicate its microenvironment pH. Three surfactants are used to study the dependence of the pH drop versus charge: cationic (cetyltrimethylammonium bromide, CTAB), anionic (sodium dodecylsulphate, SDS) and nonionic (Triton X-100) species. The decrease of CBFs pH versus the pH of the micellar solution is the following: ΔpH ≈ 1.5 for CTAB (pH: 7.0–9.0), ΔpH ≈ 0.8 for SDS, and ΔpH ≈ 0.4 for Triton X-100. With the addition of electrolyte in CBFs, we observe large decrease the amplitude of the pH anomaly, thus suggesting an electrostatic origin of the pH change at nanoscale environment.
研究领域:
生物化学
·
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Coatings Technology and Research
·
2017
·Vol. 14
·期 3
·571-582
·
IF 1.557
Jin Qingjun,Hu Yang,Shen Jie,Li Botian,Kan Chengyou
A novel green fluorescent dye, 4-hexylamino- N -(2-hydroxy-1-hydroxymethylethyl)-1,8-naphthalimide (AHHNA), was designed and synthesized, and then used as a chain extender to prepare a series of intrinsically green fluorescent polyurethane (PU) latexes. The chemical structure of AHHNA and the properties of the fluorescent PU latexes and their films were characterized. It was found that the UV–Vis and the fluorescent spectra of the PUs remained fairly consistent with AHHNA, and the fluorescence emitting capacity of 1,8-naphthalimide group was enhanced by covalently bonding AHHNA onto the PU chain. The amount of AHHNA used in the latex preparation had no obvious effect on the latex colloid properties including particle size, poly index, and zeta potential. Results showed that the color difference (Δ E ) and the extraction rate ( R
e ) of the intrinsically fluorescent PU latex film were 5.72% and 3.1%, respectively, while the Δ E and R
e of the extrinsically fluorescent PU latex film were 8.31% and 84.7%, respectively, indicating that the light fastness and the solvent fastness of the intrinsically fluorescent PU latex film were much better than those of the extrinsically fluorescent PU latex film.
研究领域:
材料化学
·
高分子科学
✓ 已验证
产品关联
DOI
高影响因子
Nature Communications
·
2023
·Vol. 14
·期 1
·1-11
·
IF 16.6
Zhang Fenghua,Li Zhong,Wang Xun
Inorganic nanowires-based organogel, a class of emerging organogel with convenient preparation, recyclability, and excellent mechanical properties, is in its infancy. Solidifying and functionalizing nanowires-based organogels by designing the gelator structure remains challenging. Here, we fabricate Ca 2 -P 2 W 16 and Ca 2 -P 2 W 15 M nanowires utilizing highly charged [Ca 2 P 2 W 16 O 60 ] 10 − and [Ca 2 P 2 W 15 MO 60 ] 14 − /13 − cluster units, respectively, which are then employed for preparing organogels. The mechanical performance and stability of prepared organogels are improved due to the enhanced interactions between nanowires and locked organic molecules. Compressive stress and tensile stress of Ca 2 -P 2 W 16 nanowires-based organogel reach 34.5 and 29.0 kPa, respectively. The critical gel concentration of Ca 2 -P 2 W 16 nanowires is as low as 0.28%. Single-molecule force spectroscopy confirms that the connections between cluster units and linkers can regulate the flexibility of nanowires. Furthermore, the incorporation of fluorophores into the organogels adds fluorescence properties. This work reveals the relationships between the microstructures of inorganic gelators and the properties of organogels, guiding the synthesis of high-performance and functional organogels.
研究领域:
材料科学
·
化学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Lijun Xu,Guimei Jiang,Hong Chen,Yue Zan,Shanni Hong,Tingting Zhang,Yuanyuan Zhang,Renjun Pei
Serum albumin has a wide range of applications in biochemical experiments and pharmaceutical field. We found that a cyanine dye , dimethylindole red (Dir), could selectively interact with bovine serum albumin (BSA). Dir exhibited very weak red fluorescence, while the fluorescence intensity at 630 nm was enhanced up to 130-fold upon noncovalently interacting with 30 µM BSA. Besides, Dir showed a highly selective response to BSA over human serum albumin (HSA). For the detection of BSA, a limit of detection as low as 23 nM was obtained. Then biocompatible Dir-BSA nanoparticles were prepared by the desolvation technique. The Dir-BSA nanoparticles possess excellent fluorescence properties with a quantum yield of 32%. Furthermore, folic acid as a targeting group was conjugated to Dir-BSA nanoparticles and these nanoparticles were characterized by TEM and laser particle analyzer, etc. Folic acid-modified Dir-BSA nanoparticles were successfully used for tumor cell-targeted imaging.
研究领域:
生物化学
·
药物学
·
肿瘤学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）