ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Quan-Quan Zhuang,Hao-Hua Deng,Shao-Bin He,Hua-Ping Peng,Zhen Lin,Xing-Hua Xia,Wei Chen
Few-atom gold nanoclusters (AuNCs) have been fabricated and used for various fields owing to their remarkable optical and photophysical features. However, the rational design for the antibody-mediated synthesis of fluorescent AuNCs for direct antigen–antibody reactions remains unexplored. In this work, immunoglobulin G (IgG)-functionalized AuNCs (IgG-AuNCs) were successfully prepared via a facile and fast biomineralization process. The generated IgG-AuNCs can emit intense red fluorescence with a high photoluminescence quantum yield. Besides strong emission, the bioactivity of IgG on the IgG-AuNCs can be retained. Surface plasmon resonance measurements suggested that IgG-AuNCs can bind to goat anti-human IgG with an affinity constant of 6.21 × 10–8 M. A simple detection method was then developed using a dot-blot immunoassay with IgG-AuNCs as fluorescent tags. Experimental results confirmed that the IgG-AuNC-based fluorescent reporters had many advantages such as low nonspecific adsorption and good photostability, offering immense potential for the development of efficient biosensors. This work can be extended to other specific antibodies to produce multifunctional AuNCs and utilized to detect and monitor targeted analytes and biological events of interest.
研究领域:
生物化学
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纳米生物学
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分析化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Yingnan Liu,Yaqing Xiao,Minghui Shang,Yuting Zhuang,Li Wang
The method of monitoring the freshness of seafood is not satisfactory for ensuring food safety. Here, we develop a filter membrane-based smart fluorescent tag with excellent biogenic amine response, which can monitor the freshness of seafood in real time and visually. We use green fluorescent fluorescein as an indicator and red fluorescent carbon quantum dots (RQDs) with aggregation-induced emission property as an internal reference. After simple mixing, they are deposited on the commercial filter membrane by physical deposition method to prepare the fluorescent tag. When amine molecules are present, the molecular conformation of fluorescein changes and the fluorescence increases rapidly, while RQDs have no obvious response, thus achieving a clear fluorescence color response to biogenic amines. Under UV light of 254 nm and 365 nm, the fluorescent tag showed sensitive, rapid, and color-responsive to ammonia in a wide range of 250–2.5 × 10 4 ppm. To further verify the practical application potential, the fluorescent tag was used to monitor the freshness of shrimp and crab in situ in real time, and satisfactory results were achieved. More importantly, owing to the reversible conformational change of fluorescein, the fluorescent tag can be reused and manufactured at low cost, making it a more attractive platform for freshness monitoring in the field of food safety.
研究领域:
食品安全
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生物化学
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分析化学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2023
·
IF 6.2
Wenyang Zhang,Da-Wen Sun,Ji Ma,Anjun Qin,Ben Zhong Tang
A ratiometric fluorescent tag based on dual-emissive hydrophobic carbon dots (H-CDs) with well volatile base nitrogens (VBNs)-response was fabricated, offering the in-situ, real-time, and visual evaluation of seafood freshness. The presented H-CDs aggregates exhibited a sensitive response to VBNs, with a limit of detection of 7 μM and 137 ppb for spermine and ammonia hydroxide, respectively. Subsequently, a ratiometric tag was successfully fabricated by depositing dual-emissive CDs on cotton paper. Upon the treatment with ammonia vapour, the presented tag exhibited highly recognizable colour variations from red to blue under UV light. In addition, the cytotoxicity was explored by CCK8 assay, and the results proved the nontoxicity of the presented H-CDs. To our knowledge, this is the first ratiometric tag based on dual-emissive CDs with aggregation-induced emission properties for recognition of VBNs and seafood freshness in a real-time and visual manner.
研究领域:
食品科学
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材料科学
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生物医学
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产品关联
DOI
PubMed
高影响因子
Nature Protocols
·
2022
·Vol. 18
·期 2
·458-489
·
IF 17.021
Liu Hao-Yang,Wang Zhi-Gang,Liu Shu-Lin,Pang Dai-Wen
Single-virus tracking (SVT) offers the opportunity to monitor the journey of individual viruses in real time and to explore the interactions between viral and cellular structures in live cells, which can assist in characterizing the complex infection process and revealing the associated dynamic mechanisms. However, the low brightness and poor photostability of conventional fluorescent tags (e.g., organic dyes and fluorescent proteins) greatly limit the development of the SVT technique, and challenges remain in performing multicolor SVT over long periods of time. Owing to the outstanding photostability, high brightness and narrow emission with tunable color range of quantum dots (QDs), QD-based SVT (QSVT) enables us to follow the fate of individual viruses interacting with different cellular structures at the single-virus level for milliseconds to hours, providing more accurate and detailed information regarding viral infection in live cells. So far, the QSVT technique has yielded spectacular achievements in uncovering the mechanisms associated with virus entry, trafficking and egress. Here, we provide a detailed protocol for QSVT implementation using the viruses that we have previously studied systematically as an example. The specific procedures for performing QSVT experiments in live cells are described, including virus preparation, the QD labeling strategies, imaging approaches, image processing and data analysis. The protocol takes 1–2 weeks from the preparation of viruses and cellular specimens to image acquisition, and 1 d for image processing and data analysis.
研究领域:
病毒学
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细胞生物学
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成像技术
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2020
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IF 8.758
Liang Huang,Jiening Jin,Lijiao Ao,Chenxing Jiang,Yuxing Zhang,Hui-Min Wen,Jing Wang,Haiyan Wang,Jun Hu
Lateral flow immunoassay (LFIA), as a prominent point-of-care (POC) test platform, has been extensively adopted for rapid, on-site, and facile diagnosis of pathogen infections and disease biomarkers. Exploring novel structured optical labels of LFIA with amplified signal and complementary detection modes favors the sensitive and flexible POC diagnosis. Here, bimodal labels with both colorimetric and fluorescent readout were fabricated via a layered sequential assembly strategy based on affinity templates and hydrophobic metal-containing nanounits. High-quality colorimetric and fluorescent nanoparticles were densely incorporated into the colloidal supports and confined in separated regions, without interfering with each other. The hierarchical integration of gold nanoparticles and quantum dots with high loading density and good optical preservation realized dual readout and amplified signals from the assemblies of individual single nanoparticles. The “all-in-one” optical labels allowed both colorimetric and fluorescent detection of cystatin C (Cys C) after surface conjugation with antibodies. The LFIA strips revealed noninterfering dual signals for both visual inspection and quantitative detection of Cys C via the naked eye and portable devices, respectively. The limits of detection by colorimetric and fluorescent modes were 0.61 and 0.24 ng mL–1, respectively. The novel LFIA platform demonstrated sensitive, specific, and reproducible POC testing of biomarkers with flexible detection modes and was reliable for clinical diagnosis.
研究领域:
生物医学工程
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分析化学
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纳米技术
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诊断医学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Yingnan Liu,Wanqi Jiang,Zan Yang,Fei Tong,Lei Wang,Lu Zhu,Zhenyu Yu,Mingming Zheng,Yaqing Xiao,Yibin Zhou
A zein-based film functionalized with fluorescent dyes was constructed for visual, real-time monitoring of seafood freshness. The addition of fluorescent dyes had no significant effect on the mechanical property or water sensitivity of the zein-based film, and the fluorescent film exhibited dual emission response. When exposed to ammonia, fluorescein fluorescence increased whilst the fluorescence of rhodamine B was undisturbed. The fluorescent film presented a clearly distinguishable color transition from pink to green. Sample detection tests showed the fluorescent film served successfully as a response label for on-site visual detection of seafood freshness. Development of this intelligent fluorescent film provides a new platform for food safety and quality monitoring, benefiting economic value and public health.
研究领域:
食品科学
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材料科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2021
·
IF 3.764
Wenli Liu,Qizhi Yang,Yili Yang,Feiyue Xing,Pu Xiao
In the past decades, polymeric fluorescent materials with aggregation-induced emission (AIE) properties play important roles in the fields of biomedical and analytical chemistry, and the synthesis of well-defined polymers under mild conditions is in demand. In this report, narrowly distributed poly(methyl methacrylate)s (PMMAs) with AIE properties were prepared in mild conditions using 4-(1,2,2-triphenylvinyl)benzyl 2-bromo-2-phenylacetate (TPE-BPA), a novel initiator with an AIE-based functional group. This approach resulted in PMMAs with a fluorescent tag at the chain end (TPE-PMMA). Specifically, a high efficiency of initiation was observed using a low amount of air-stable copper(II) bromide/tris(2-pyridylmethyl)amine (CuIIBr2/TPMA) as a photocatalyst under the irradiation of LED@405 nm. Moreover, the TPE presented in the system did not affect the polymerization kinetics of MMA and the temporal control. The dispersities of the obtained PMMAs were as low as 1.12. Interestingly, TPE-PMMA with narrow dispersity was also obtained (Mw/Mn < 1.25) in the presence of air. Regarding the fluorescent properties of TPE-PMMAs, it was observed that their AIE intensities increased with the molecular weights. Moreover, they could also be used to probe the increased viscosity of the solution, indicating their potential of synthesizing functional sensors. The approach proposed in this work is convenient to prepare poly(methacrylate)s with well-defined structures in the presence of AIE-based fluorescent tags, and it thus paves a new way for the rational design of AIE-functionalized polymeric materials.
研究领域:
高分子化学
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材料科学
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分析化学
·
生物医学工程
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产品关联
DOI
高影响因子
FOOD CONTROL
·
2024
·
IF 5.6
Xianghong Xie,Bangfeng Fu,Mingyu Zhang,Wentao Zhang,Zhenghua Xu,Shuwen Liu,Jianlong Wang
The implementation plan of extending the shelf life of food while ensuring food safety is playing an increasingly important role in promoting global solutions to food waste. Here, we have logically created a new eugenol nanoemulsion to sense spoilage and extend the shelf life. The nano lotion (Eu/SA) was loaded with sodium alginate nanoparticles as carrier, eugenol as antibacterial agent and antioxidant. Fluorescein isothiocyanate (FITC) was utilized concurrently as an indicator by simply mixed with Eu/SA nanoemulsion, then physically deposited on a commercial filter membrane to create smart fluorescent labels. Shrimp with and without the Eu/SA nanoemulsion coating had their freshness monitored using a non-contact tagging technique. And through smartphone integrated with RGB (red/green/blue) analysis for digitization, it was found that the RGB of uncoated shrimp was greater than that of treated shrimp. Additionally, without coating treatment, thiobarbituric acid (TBA), pH and viable bacterial count (TVC) of Shrimp all exceed the established. Eu/SA nanoemulsion offers a more appealing monitoring and preservation integration platform for the food safety industry.
研究领域:
食品科学
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纳米技术
·
生物技术
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化学工程
·
传感器与执行器
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产品关联
DOI
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2021
·Vol. 31
·期 16
·2009916
·
IF 18.808
Ruilian Chen,Dengchong Feng,Guojian Chen,Xudong Chen,Wei Hong
The construction of invisible patterns via high-resolution printing and the independent encoding/decoding of complex information can lead to promising applications in steganography and watermarking for optical encryption. Herein, a rewritable chiral photonic paper formed by cholesteric cellulose nanocrystals and polycation is reported. The chemically crosslinked polycation network interpenetrates in the cholesteric structure while retaining the optical properties of the photonic crystals. The film exhibits controllable wettability via anion exchange, leading to extremely low contrast in the dry state but high contrast by a rapid wetting response. Triple invisible information is independently encoded on the films, including invisible patterns caused by reversible counterion-controlled wettability, permanent fluorescent labels based on fluorescent counterions, and polarization-dependent structural colors based on cholesteric structures. Full color patterns can be reversibly constructed via inkjet printing, with a high resolution of 100 µm. In addition, the circular polarization characteristics of the cellulose nanocrystals, liquid crystals, endow the system with complex and independent responses, realizing a wetting/polarization double-key decryption. This work provides a simple and effective optical technique for coding complex information on a single material platform and expands the techniques available to achieve invisible patterns for sensing and anti-counterfeiting.
研究领域:
材料科学
·
光学
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纳米技术
·
密码学
·
安全
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产品关联
DOI
高影响因子
ANALYTICAL SCIENCES
·
2019
·Vol. 36
·期 6
·681-685
·
IF 1.618
Liu Wenwen,Ma Ligang,Guo Zhuangzhuang,Liu Tao,Liu Yanju,Wang Dazhong,Kong Jinming
We propose a novel, ultrasensitive and low-cost sensor using reversible addition-fragmentation chain transfer (RAFT) polymerization as a signal amplification strategy for the detection of CYFRA 21-1 DNA fragment, a tumor marker of non-small cell lung carcinoma. The peptide nucleic acid (PNA) probes were firstly immobilized on magnetic beads (MBs) to capture the CYFRA 21-1 DNA specifically. After hybridization, CPAD was tethered to the hetero duplexes through carboxylate-Zr 4+ -phosphate chemistry. Subsequently, a number of fluorescent tags were introduced to the heteroduplexes through RAFT polymerization, leading to an amplification of the fluorescence signal. The sensor demonstrates a low limit of detection (LOD) of 0.02 fM. It has great selectivity with respect to base mismatch DNA, and high anti-interference ability in normal human serum. Overall findings of the study suggest that proposed sensor holds enormous potential to be used as a tool for the early-stage diagnosis of lung cancers.
研究领域:
生物技术
·
癌症诊断
·
纳米技术
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）