ACS Nano
·
2015
·
IF 12.881
Kai Han,Shi-Bo Wang,Qi Lei,Jing-Yi Zhu,Xian-Zheng Zhang
Photodynamic therapy faces the barrier of choosing the appropriate irradiation region and time. In this paper, a matrix metalloproteinase-2 (MMP-2) responsive ratiometric biosensor was designed and synthesized for aggregation-induced emission (AIE)-guided precise photodynamic therapy. It was found that the biosensor presented the MMP-2 responsive AIE behavior. Most importantly, it could accurately differentiate the tumor cells from the healthy cells by the fluorescence ratio between freed tetraphenylethylene and protoporphyrin IX (PpIX, internal reference). In vivo study demonstrated that the biosensor could preferentially accumulate in the tumor tissue with a relative long blood retention time. Note that the intrinsic fluorescence of PpIX and MMP-2-triggered AIE fluorescence provided a real-time feedback which guided precise photodynamic therapy in vivo efficiently. This strategy demonstrated here opens a window in the precise medicine, especially for phototherapy.
研究领域:
癌症研究
·
治疗诊断学
·
生物医学工程
·
分子影像
✓ 已验证
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Huifang Wu,Yuwei Ling,Shiying Ju,Yubing Chen,Mengqi Xu,Yanfeng Tang
Phosphate (Pi) plays an essential role in aquatic ecosystems as well as in physiological processes. Here, a dual-emission probe for the sensitive, specific and visual analysis of Pi is fabricated by coordinating Eu 3+ with luminol and 2,6-pyridinedicarboxylic acid (DPA). Pi can significantly enhance the characteristic fluorescence of Eu 3+ at 615 nm by promoting energy transfer from DPA to Eu 3+ and reducing the quenching effect of water molecule, luminol with inherent emission at 423 nm further enhances the Eu 3+ fluorescence. Accordingly, ratiometric detection of Pi can be achieved with the fluorescence ratio F 615 /F 423 as a function of Pi concentration. Linearity between F 615 /F 423 and Pi concentration in the range of 0.1–25 μM is shown, and the limit of detection (LOD, 3σ/K) for Pi is 0.027 µM. In addition, a continuous change in the fluorescence color of the probe from blue to red is observed with increasing Pi concentration under a UV lamp, and a smartphone-based visual method is used for the convenient and effective semi-quantitative determination of Pi. The dual-emission probe has been successfully applied to ratiometric and visual analysis of Pi in human urine and environmental water samples, and adequate results are obtained.
研究领域:
分析化学
·
环境科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Yawen Rong,Md Mehedi Hassan,Qin Ouyang,Li Wang,Tianhui Jiao,Quansheng Chen
The current work puts forward a turn-off ratiometric upconversion fluorescence nanosensor for the quantification of furfural (FUR). Upconversion nanoparticles (UCNPs) containing Y 3+ , Yb 3+ , and Er 3+ were synthesized; mesoporous SiO 2 and amino groups were subsequently conjugated and functioned as the signal component. The p-toluidine molecule played the role of recognition molecules, which coupled with UCNPs to form a unique detection system. An absorption peak appeared when the FUR was combined with p-toluidine resulted in an obvious quenching of upconversion emission at 539 nm via inner filter effects (IFE), while the fluorescence peak at 653 nm maintained constant. Therefore, the FUR concentration was quantified by the record of fluorescence ratio I 539 / I 653 . Under optimal conditions, the proposed UCNPs-p-toluidine coupled nanosystem achieved the ratiometric fluorescence sensing toward FUR in the linear range of 0.01–100 μg/mL with a detection limit as low as 0.003 μg/mL. The accuracy of the sensor was validated by the HPLC method with preferable accuracy. Finally, the overall nanosystem showed excellent application potential in adulterated foodstuff (e.g., cookie, honey, fruit wine, milk tea, and potato chips).
研究领域:
分析化学
·
食品科学
·
纳米技术
·
光谱学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Cong Dai,Biao Gu,Si-Ping Tang,Pei-Hong Deng,Bo Liu
Fluorescent porous organic cage with good water solubility is of great interest but still challenging for its fluorescent sensing application. Poor water solubility and single signal of most previous probes are unfavorable for the monitoring of Au 3+ generated from the potential dissociation of gold nanoparticles in environmental and biological samples. Here we report a water-soluble porous organic cage as a ratiometric fluorescent probe for Au 3+ in aqueous solution. The prepared porous organic cage with good water solubility showed specific redox interaction with Au 3+ in pH 5, leading to the change of dual emission at 420 and 484 nm. Based on the change of fluorescence ratio, a simple ratiometric sensing method for Au 3+ from the dissociation of gold nanoparticles in aqueous solution was developed. The proposed method gave a calibration function of F 484 /F 420 = 0.0370[Au 3+ ] + 0.5689 (where F 484 /F 420 is the intensity ratio of fluorescence at 484 nm to that at 420 nm; [Au 3+ ] in μM) (R 2 = 0.9975) in the concentration range of 1–60 μM, the limit of detection ( 3s ) of 8 nM, and the relative standard deviation of 0.26% for 10 replicate detections of 50 μM Au 3+ . The recoveries of spiked Au 3+ in domestic wastewater and human serum samples ranged from 94.66% to 105.61%.
研究领域:
分析化学
·
环境科学
·
材料科学
·
光谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）