TALANTA
·
2025
·
IF 6.1
Lijun Wang,Jiangchuan Tang,Shanshan Ren,Haixia Hu,Xiao Yang,Xianggui Chen
Although many colorimetric methods have been developed for on-site pesticide detection, most exhibit low sensitivity and limited resistance to interference. To address these issues, this study developed a novel dual-mode colorimetric-ratiometric fluorescence method for isocarbophos (ICP) detection using sulfur and nitrogen co-doped carbon nanodots (S, N-CDs) to inhibit catalytic Fe 3 O 4 @Cu-BTC nanozyme activity. The synthesized Fe 3 O 4 @Cu-BTC nanozymes with higher peroxidase-like activity were inhibited by S, N-CDs. The inhibitory mechanism was deeply investigated and was ascribed to (1) the restriction of the reaction between Fe 3 O 4 @Cu-BTC and hydrogen peroxide (H 2 O 2 ), which decreased the ·OH production, and (2) the ·OH scavenging ability of the S, N-CDs, forming the basis of the dual-mode method. The Fe 3 O 4 @Cu-BTC/Apt-complementary strand (CS)/S, N-CDs hybridization complexes demonstrated minimal to no peroxidase-like activity, preventing H 2 O 2 from oxidizing o -phenylenediamine (OPD) to produce yellow fluorescent 2,3-diaminophenazine (DAP). Meanwhile, the detection system demonstrated a low fluorescence intensity ratio (I 575 /I 465 ) at an excitation wavelength of 390 nm. However, the presence of ICP caused the CS/S, N-CDs to separate from the Fe 3 O 4 @Cu-BTC/Apt due to the high affinity between Apt and ICP. Then, the Fe 3 O 4 @Cu-BTC/Apt-ICP complexes recovered peroxidase-like activity, which promoted DAP production, accompanied by a pronounced fluorescence intensity ratio (I 575 /I 465 ). In optimum conditions, the detection limits (LODs) were 2.79 ng/mL (colorimetric) and 0.26 ng/mL (ratiometric fluorescence), respectively. The feasibility of the proposed method was verified via three spiked samples, yielding recovery rates of between 86.08 % and 112.64 %, indicating the significant potential for the highly sensitive, accurate on-site detection of ICP in actual samples.
研究领域:
分析化学
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环境科学
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生物医学
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Jingyu Pang,Ruyan Xie,Sophie Chua,Yu Zou,Mingyu Tang,Fang Zhang,Fang Chai
A dual-mode colorimetric and fluorimetric probe was successfully established based on silver/copper bimetallic nanoparticles (AgCu-BNPs). The AgCu-BNPs were confirmed as individually bimetallic nanoparticles with a mean size of 7.7 ± 0.2 nm, as characterized by high resolution transmission electron microscopy. Intriguingly, the AgCu-BNPs possess both surface plasmon resonances (SPR) and fluorescence emission. AgCu-BNPs emanate bright blue fluorescence with optical emission centered at 442 nm with high quantum yield of 30.3%, and AgCu-BNPs were attenuated or even quenched by Hg 2+ via both static and dynamic quenching, coincidently accompanied by a visible color change, which endow AgCu-BNPs a unique utility as dual-mode colorimetric and fluorimetric probes. The detection limits as low as 89 nM and 9 nM were determined by dual-mode of AgCu-BNPs, respectively. The recovery rates in real samples were found to be 97.3–118.8%, and 89.5–112.7% by colorimetric and fluorescent methods separately, demonstrates the good environmental tolerance of the dual-mode probe.
研究领域:
分析化学
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环境科学
·
纳米技术
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高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2021
·
IF 4.556
Qin Hu,Yikun Cui,Longfei Zhang,Minjie Qian,Lixia Xiao,Ming Yang,Zhen-Quan Yang,Shengqi Rao,Xiaojuan Gong,Jie Han
In this work, a novel fluorescence method based on an orange-emissive carbon dots (O-CNDs) was for the first time reported for the rapid and ultrasensitive determination of malachite green (MG). The O-CNDs were simply produced via a one-pot hydrothermal treatment of rhodamine B. The detection mechanism was based on the interaction between O-CNDs and MG, where the fluorescence of O-CNDs was suppressed by MG via a combined effect of inner filter effect (IFE) and dynamic suppressing. Under the optimum sensing conditions, the O-CNDs were found to display rapid reaction time (1.0 min), high selectivity and excellent sensitivity with two linear concentration ranges of 0.01–0.1 and 0.1–10.0 μmol L −1 , and the corresponding detection limits were as low as 0.08 and 0.93 nmol L −1 , respectively. The developed fluorescence method was successfully applied for the determination of MG in fish samples, including silver carp, blackfish, blunt-snout bream, common carp and grass carp, with satisfactory results; the recoveries were in a range of 96.0–104.8 % and the relative standard deviations (RSDs) below 4.54 %. The results of this study validated the great potential of O-CNDs as an effective fluorescence sensing agent for MG monitoring in aquatic samples.
研究领域:
分析化学
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环境科学
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Shuo Geng,Shu Min Lin,Shi Gang Liu,Wei Zhang,Nian Bing Li,Hong Qun Luo
We synthesized polyethyleneimine capped ZnO quantum dots (PEI-ZnO QDs) which dispersed well in ethanol by using a two-step method. UV–visible absorption spectroscopy, photoluminescence, Fourier transform infrared spectroscopy, high resolution transmission electron microscope, and field emission scanning electron microscope were used to characterize the as-prepared PEI-ZnO QDs. It emitted green-yellow fluorescence (527 nm) for excitation at 355 nm. We used the PEI-ZnO QDs to determine the alcoholic strength because of its different property in water and ethanol which caused decrease in the PEI-ZnO QDs fluorescence intensity with increasing water content. With this method, we determined the alcoholic strength of different artificial samples with a linear range from 20% to 60% vol and detected commercial spirits with satisfactory results.
研究领域:
化学
·
材料科学
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高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Chenyang Yue,Lintao Zeng,Di Zhang,Kai Li,Lirong Jiang,Peng Xie
Sulfur dioxide (SO 2 ) and its derivatives (HSO 3 − and SO 3 2− ) are widely used in food-processing. Whereas excessive consumption of sulfur dioxide and its derivatives (>0.70 mg·kg −1 day −1 ) severely endangers human health. In this work, we rationally constructed a practical dual-mode probe (dicyanomethylene)-1-methyl-1,4-dihydroquinolin-2-yl)vinyl)-1-methylquinolinium (QMN), which underwent a specific 1, 4-Michael addition with sulfite to afford a noticeable color change from pale yellow to red along with a high-contrast fluorescence turn-on response at 598 nm. QMN has the advantages of rapid response, high signal-to-noise ratio, excellent selectivity, good water-solubility, large Stokes shift and low detection limit (LOD = 31.9 nM). QMN has been successfully used to on-site visually determine sulfite in a diversity of foods with satisfactory recoveries (91.33–111.33 %) and high accuracy (93.74–98.71 %). Furthermore, a portable smartphone-based fluorescence sensing platform was fabricated for on-site determination of sulfite in food with good performance.
研究领域:
分析化学
·
食品安全
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DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Wanchao Chen,Yinran Xiong,Wenming Wang,Ting Wu,Long Li,Qidi Kang,Yiping Du
A simple and sensitive fluorescence method has been developed for the determination of amiloride (AMI) in pharmaceutical tablet and human serum with a portable, cost-effective, and easy-to-operate fluorescence system. The fluorescence system was assembled with some optical and electronic devices mainly including a 370 nm light-emitting-diode (LED) as light source, a fiber spectrometer and a Raspberry Pi computer. With the system, AMI produced a strong fluorescence emission at 413 nm, and the emitted intensity can maintain good stability with in a wide pH range from 2 to 8. The proposed method was successfully applied to the determinations of AMI in pharmaceutical tablet and human serum. Their detection limits were 1.67 ng mL −1 and 1.43 ng mL −1 respectively, and the recovery was in the range of 94.06–114.0%. Those obtained results proved that the proposed methods combined with the developed fluorescence system could be employed for the routine analysis of amiloride in pharmaceutical tablet and human serum, especially for the fast analysis of them under field conditions.
研究领域:
分析化学
·
药物分析
·
生物医学工程
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2016
·
IF 2.653
Li Wang,Hai-Long Wu,Xiao-Li Yin,Yong Hu,Hui-Wen Gu,Ru-Qin Yu
A chemometrics-assisted excitation-emission matrix (EEM) fluorescence method is presented for simultaneous determination of umbelliferone and scopoletin in Tibetan medicine Saussurea laniceps (SL) and traditional Chinese medicine Radix angelicae pubescentis (RAP). Using the strategy of combining EEM fluorescence data with second-order calibration method based on the alternating trilinear decomposition (ATLD) algorithm, the simultaneous quantification of umbelliferone and scopoletin in the two different complex systems was achieved successfully, even in the presence of potential interferents. The pretreatment is simple due to the “second-order advantage” and the use of “mathematical separation” instead of awkward “physical or chemical separation”. Satisfactory results have been achieved with the limits of detection (LODs) of umbelliferone and scopoletin being 0.06 ng mL − 1 and 0.16 ng mL − 1 , respectively. The average spike recoveries of umbelliferone and scopoletin are 98.8 ± 4.3% and 102.5 ± 3.3%, respectively. Besides, HPLC-DAD method was used to further validate the presented strategy, and t- test indicates that prediction results of the two methods have no significant differences. Satisfactory experimental results imply that our method is fast, low-cost and sensitive when compared with HPLC-DAD method.
研究领域:
中药分析
·
化学计量学
·
药物分析
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PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Bodong Wang,Shan Pu,Chengyan Xia,Xiandeng Hou,Kailai Xu
Background Recently, headspace single-drop microextraction (HS-SDME) has attracted some attention for developing sensitive and selective colorimetric assays due to its excellent capability to reduce matrix interference and enrich analytes. However, the single droplet limits direct visual observation of color change and its quantitative measurement suffers from reduced optical path length. Therefore, amplifying the detection signals in both volume and intensity is an important and challenging task for improving the sensitivity, stability, and accuracy of such colorimetric analysis . Results In this study, a "headspace-nanoenzyme" (HS-NE) strategy was proposed that successfully addressed these challenges and enabled the colorimetric and fluorescent dual-mode detection of trace Hg 2+ . Atomic Hg 0 , generated via chemical vapor generation (CVG), underwent headspace reaction with AuNPs droplet to form Au@HgNPs, thus catalyzing the oxidation of o -phenylenediamine (OPD) in the presence of H 2 O 2 . The absorbance and fluorescence intensity of oxidized OPD were proportion to the concentration of Hg 2+ in the sample solution. Due to the greatly enhanced peroxidase-like activity by Au@HgNPs, the limit of detection was as low as 0.98 nM and 0.21 nM for the colorimetric and fluorescent modes, respectively. The applicability of this assay was further demonstrated with determination of Hg 2+ in real environmental and biological samples. Moreover, a convenient and cost-effective paper-based sensing platform was fabricated for rapid on-site detection of Hg 2+ . Significance and novelty This novel HS-NE strategy combines HS-SDME and nanoenzyme-based sensing to achieve dual effects of eliminating matrix interference and amplifying the measurement signal, resulting in improved accuracy, enhanced stability, high sensitivity, and exceptional selectivity , with great potential for on-site determination of trace Hg 2+ .
研究领域:
分析化学
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环境科学
·
生物医学
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Peihua Li,Na Liang,Chao Liu,Lian Xia,Fengli Qu,Zhi-Ling Song,Rong-Mei Kong
In this work, a novel silver ion (Ag + )-regulated ratiometric fluorescence method for the effective and sensitive determination of alkaline phosphatase (ALP) was established based on carbon dots (CDs) and o -phenylenediamine (OPD). OPD can be oxidized by Ag + to generate fluorescent 2, 3-diaminophenazine (DAP). Thus, based on inner-filter effect (IFE) or/and fluorescence resonance energy transfer (FRET) between CDs and DAP, the CDs-Ag + -OPD system can generate dual-emission at 454 nm and 570 nm respectively when excited at 360 nm. The introduction of ascorbic acid (AA) can react with Ag + to produce dehydroascorbic acid (DHAA), which inhibits the generation of DAP, resulting in the fluorescence decrease at 570 nm and fluorescence recovery of CDs at 454 nm. Meanwhile, DHAA can react with OPD to generate quoxaline (QX), which emits strong blue fluorescence at 440 nm, further inhibiting the IFE or/and FRET between CDs and DAP. An obvious ratiometric fluorescence response was observed with the increase of the concentration of AA introduced. Due to the fact that AA can be generated by the enzyme catalysis reaction between ALP and 2-phospho- l -ascorbic acid (AAP), the CDs-Ag + -OPD ratiometric system was applied to the determination of ALP successfully. The ratiometric fluorescence value of F 454 / F 570 increases with increasing ALP concentration, with a linear range of 0.2 to 40 U/L and detection limit of 0.1 U/L. In addition, the CDs-Ag + -OPD ratiometric system was successfully applied to the detection of ALP in human serum samples.
研究领域:
分析化学
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生物医学
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Lanmei Zhang,Yuqi Dai,Erzheng Su,Yunfeng Pu,Lu Li,Lanling Chu
A novel unlabeled aptamer-based fluorimetric method combined with fiber-packed solid-phase extraction (FPSPE) was developed for the detection of aflatoxin B 1 (AFB 1 ). Polystyrene-cellulose acetate butyrate-montmorillonite K-10 (PS-CAB-MMTK-10) nanofibers were prepared by electrospinning method and used as adsorbent for solid phase extraction of AFB 1 . Then the types of nanofibers, types of activation solution, pH value of sample, types of eluent, amount of packed fibers, eluent volume and extraction time were studied and optimized. Under optimal conditions, the method was shown a preferable linear relationship in the range of 0.5–200 nM, and the limit of detection (LOD) was 0.3 nM, and the recovery rate was 85.1–91.2 %. This proposal method realized the detection of AFB 1 based on the different fluorescence intensities caused by the different contents of the target substance in the detection system. The method does not require covalent labeling aptamers, and it is simple to operate to apply to the rapid detection of AFB 1 in food.
研究领域:
分析化学
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食品安全
·
生物传感器
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高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 8
·1-12
·
IF 5.3
Ruan Ya,Li Qiulan,Yang Dezhi,Yang Yaling
Copper selenide nanoparticles (CuSeNP) were synthesized using histidine, ethylenediamine, and sodium selenate as precursors by one-step microwave digestion methods. The as-prepared CuSeNPs exhibit excellent catechol oxidase mimic enzyme and catalase (CAT)-like activities. Dopamine (DA) can be oxidized to aminochrome with H 2 O 2 by CuSeNPs, and the intermediate product aminochrome can further react with α -naphthol to yield a highly fluorescent derivative. It was confirmed that Cr(III) could adsorb on the surface of CuSeNPs and inhibit the production of semiquinone radicals in the reaction system, and the catalytic activity of CuSeNPs was inhibited. The detection mechanisms, kinetics, and catalytic properties of CuSeNPs were systematically investigated. As a result, a novel fluorescence method for the assay of Cr(III) was established. The feasibility of CuSeNP nanozyme in detecting speciation Cr(III) in food samples was explored with satisfactory results. It showed the obvious potential for developing effective and dependable fluorescent detection method for protecting food safety. Graphical Abstract
研究领域:
分析化学
·
食品安全
·
纳米生物学
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PubMed
高影响因子
Nanoscale
·
2025
·
IF 5.1
Zuyao Fu,Lingfeng Yang,Zhaoyang Ding,Jing Xie
Immobilized enzymes play a crucial role in analytical sensing due to their exceptional stability and considerable commercial importance. In this study, a stable Zr-based metal–organic framework (UiO-66-NH2) was prepared as an immobilization platform for horseradish peroxidase (HRP) through covalent binding. HRP@UiO-66-NH2 retained 75% of its activity after 10 cycles. Subsequently, a colorimetric/fluorometric dual-mode sensing strategy using HRP@UiO-66-NH2 was developed for nitrite detection. HRP@UiO-66-NH2 facilitated the transformation of the non-colored compound 3,3′,5,5′-tetramethylbenzidine (TMB) into its blue oxidized form. When nitrite is added, oxidized TMB (ox-TMB) specifically engaged with nitrite (NO2−) to generate diazotized TMB, leading to a color shift from blue to yellow. Concurrently, NO2− reacted with the amino groups of HRP@UiO-66-NH2, forming diazonium compounds and suppressing the fluorescence of HRP@UiO-66-NH2. The limits of detection were 0.21 μM and 0.19 μM for the colorimetric and fluorometric strategies, respectively. Furthermore, a portable kit for detecting nitrite was created by integrating gelatin with HRP@UiO-66-NH2. The kit could visually identify nitrite from 10–400 μM in colorimetric mode and 0–400 μM in fluorometric mode. This method provides an innovative approach for nitrite sensing, paving the way for new research into multifunctional immobilized enzymes and their potential uses in biochemical sensing applications.
研究领域:
生物化学
·
分析化学
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PubMed
高影响因子
危险品化学品经营许可证（带存储）