LAB ON A CHIP
·
2025
·
IF 5.4
Wanting Hu,Hua Sun,Huibo Qi,Linkai Jiang,Kaining Zhang,Xiaomeng Jia,Yu Wang,Yu Xiang,Qionglin Liang
Glioblastoma is one of the most malignant tumors in the world, but the development of its therapies remains limited. Herein, a microfluidic chip that mimics the cerebrospinal fluid (CSF) circulation microenvironment is proposed to study the migration characteristics of glioblastoma U87-MG cells and U251 cells in complex environments where glioblastoma coexists with diseases that elevate CSF levels. In the presence of interstitial flow (IF), changing both cell densities and the cellular environment results in increased cell motility, including an increase in the number of migrating cells, the mean displacement of the top 30% fastest-moving cells, and the overall mean displacement. Then, through dynamic migration characterization analysis, it was found that IF enhances cell velocity and speed. Importantly, cells exposed to IF tend to migrate in directions with smaller angles of deviation from the opposite direction of IF. Finally, cytoskeleton inhibitors and decreased expressions of focal adhesion proteins, such as cytochalasin D, FAK inhibitors (VS-6063 and PF-573228), and FAK siRNA, were both proved to decrease the cells' response to IF. This work not only demonstrates the effect of IF on glioblastoma cell migration, but also indicates the reliability of microfluidic chips for modeling complex physiological environments, which is expected to be further developed for drug screening.
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EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
·
2026
·
IF 5
Junming Li,Xinxin Liang,Shuo Ma,Jingxin Gou,Tian Yin,Haibing He,Xing Tang,Jiansong Zhao,Yu Zhang
Tacrolimus (TAC), as a new potent immunosuppressant, holds promise for treating immune-mediated ocular surface diseases, especially in dry eye disease (DED) and allergic conjunctivitis (AC). However, the low water solubility of TAC results in commercial formulations (Talymus®) as suspensions with limited corneal retention time and low penetration efficiency. In addition, the irritation of TAC reflexes tearing and rapid drug clearance, thereby reducing bioavailability. To address these challenges, we developed a TAC cationic nanoemulsion (TAC-CNE) with good biocompatibility. The oil-phase encapsulation improved water solubility while reduced irritation of TAC, and enabled sustained drug release via diffusion kinetics. Furthermore, cationic surface modification promoted electrostatic interactions with negatively charged ocular mucin proteins. This interaction, combined with the appropriate viscosity, can prolong the ocular surface retention time and thereby improve bioavailability. In addition, TAC-CNE was prepared through a simple high-pressure homogenization method, exhibiting stability over 6 months under storage conditions. In vivo and in vitro evaluations demonstrated the superior therapeutic efficacy of TAC-CNE in DED and AC. In particular, TAC-CNE was more prominent in safety, which solved the problems of ocular irritation of commercial preparations. Our findings highlight TAC-CNE as a promising strategy to overcome the limitations of commercial formulations, offering a new treatment choice for DED and AC. Download: Download high-res image (132KB) Download: Download full-size image
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DENTAL MATERIALS
·
2026
·
IF 6.3
Zhenzhen Zhang,Chang Shu,Shengli Hu,Jingheng Wu,Yadong Chen,Yanji Lu,Qi Zhong,Mengqi Pan,Jiajia Xu,Zhuo Chen,Xiaodong Li,Qiaojie Luo
CaCl₂/AgF treatment achieves rapid ex vivo dentinal tubule occlusion within 40 s. • Co-precipitation generates deep intratubular deposits (∼96 μm). • Occlusion remains stable after brushing, ultrasonication, and acidic challenges. • CaCl₂/AgF treatment reduces fluid transport under simulated pulpal pressure. • The Ca–Ag strategy shows promise for durable dentinal tubule occlusion. Objectives Dentin hypersensitivity arises from fluid movement within patent dentinal tubules. Existing chemically mediated occlusion strategies require days to weeks, limiting clinical applicability. This study developed a rapid CaCl₂/AgF regimen for durable dentinal tubule occlusion. Methods Human third molars were acid-etched with 37% phosphoric acid for 15 s to establish an exposed dentin model. Specimens were sequentially treated with 0.05 M CaCl₂ and 0.1 M AgF for 20 s each (Ca–Ag group). Gluma, Duraphat, and Actimins served as comparators. Tubule occlusion, surface hardness, resistance to abrasive, ultrasonic, and acidic challenges, and fluid transport through pulp were evaluated. Results The Ca–Ag regimen achieved near-complete tubule occlusion (97.5 ± 1.4%) with deep intratubular deposits (96.0 ± 10.5 μm), significantly outperforming commercial desensitizers ( p < 0.001). Tubule sealing remained stable after abrasive (92.2 ± 4.3%), ultrasonic (93.8 ± 2.8%), and acidic (95.2 ± 1.9%) challenges. Surface hardness increased from 34.5 ± 2.8 HV to 46.3 ± 3.7 HV. Under 15 cm H 2 O pulp pressure, Ca–Ag markedly reduced fluid penetration, decreasing the mean fluorescence intensity from 173.9 ± 14.8–37.4 ± 6.5 AU ( p < 0.001), whereas minimal reductions were observed in the other groups. Conclusions CaCl₂/AgF treatment enables rapid, deep, and durable dentinal tubule occlusion with strong resistance to mechanical and chemical challenges. Significance The Ca–Ag regimen provides a rapid and durable ex vivo dentinal tubule occluding strategy. The clinical desensitizing efficacy and long-term performance require validation through well-controlled in situ and clinical trials.
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Foods
·
2026
·Vol. 15
·期 14
·2490
·
IF 6
Yue Tian,Wenting Yang,Yangzheng He,Xin Bi,Yong Sun
Rice protein peptides, abundant byproducts of rice processing, represent a sustainable source for developing novel nutritional delivery systems. To address the low bioavailability of traditional calcium supplements, this study aimed to fabricate a high-performance calcium-chelating complex (RPP-Ca) and elucidate its functional mechanism. The synthesis process was systematically optimized, yielding a maximum calcium-binding capacity of 93.98 ± 1.99 mg/g under optimal conditions (pH 10, 70 °C, 50 min reaction time, peptide-to-calcium mass ratio of 2:1). Physicochemical characterization utilizing scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) confirmed successful chelation, revealing significant microstructural reorganization and enhanced thermal stability compared to native peptides. Functional validation via in vitro Caco-2 cell models and in vivo calcium-deficient mouse models demonstrated that RPP-Ca significantly promotes intestinal calcium absorption and osteogenesis. Mechanistically, these effects were mediated through the activation of the TRPV6-Calbindin9k signaling axis. These findings underscore the potential of industrial rice protein peptides as an effective and bioavailable calcium fortification ingredient, providing a theoretical basis for the high-value utilization of rice byproducts in functional foods.
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EUROPEAN POLYMER JOURNAL
·
2026
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IF 6.8
Shiyun Lin,Jinghua Tan,Qihang Xv,Deqiang You,Sheng Cao,Xiaojian Wang
Ureteral stents are critical medical devices for the treatment of urinary obstruction, facilitating urine drainage and postoperative recovery. Clinically, stents are prone to bacterial adhesion and colonization, which leads to urinary tract infections in up to 30%–50% of patients. Meanwhile, minerals in urine tend to readily deposit on their surfaces and form encrustations. To address these issues, we developed a multifunctional double-network hydrogel coating, (P(AAm-co-SBMA)/SA-Ca 2+ ), through physical and chemical crosslinking. The crosslinking density of the hydrogel was optimized by adjusting the SBMA concentration. Compared with uncoated polyurethane ureteral stents, the coated stents exhibited a low friction coefficient of 0.28, a reduction of over 83%, which significantly improved surface lubricity. More importantly, the coated ureteral stents showed synergistic antibacterial adhesion (up to 90%) and anti-encrustation deposition properties. In vitro simulated urine experiments demonstrated that encrustation deposition was reduced by 84.46% after short-term immersion (14 days) and by 75.48% after long-term immersion (21 days). This novel coating provides an effective surface modification solution for solving the problems of ureteral stent infection and encrustation formation, holding significant clinical translation value.
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DRUG DELIVERY
·
2026
·
IF 8
Yu-Xin Guo,Qing Deng,Ke Li,Quan Zhang,Jie Guo,Meng-Fei Wang,Jia-Lin Wu,Hui-Mei Lei,Yue-Ying Chen,Huan Pu,Yu-fan Gao,Yong-Hong Xu,Xiao Chen,Qing Zhou
Glioblastoma (GBM), the deadliest primary brain malignancy, resists treatment because of the blood‒brain barrier (BBB) and intrinsic resistance mechanisms. Fluorescein (FL), a clinically approved fluorophore, shows promise as a sonosensitizer for sonodynamic therapy (SDT), but faces challenges in tumor delivery and incomplete mechanistic understanding beyond ROS-mediated damage. We developed a bioinspired platelet-based delivery platform exploiting the natural tumor-homing properties of platelets for targeted FL delivery. Platelets were loaded with fluorescein diacetate (FDA), a lipophilic prodrug that is converted intracellularly to active FL, combined with probenecid to inhibit MRP1-mediated efflux, generating the ultrasound-responsive FL@plt. In vitro, FL demonstrated sonocytotoxicity; upon low-intensity ultrasound, FL@plt rapidly released FL into GBM cells, producing significant cytotoxicity enhanced by probenecid-mediated retention. In subcutaneous and orthotopic mouse models, intravenous FL@plt with probenecid and sequential ultrasound achieved extensive tumor FL accumulation, massive necrosis, significant growth inhibition, and prolonged survival. Mechanistically, FL-SDT induces DNA damage while promoting HIF-1α degradation via the ubiquitin‒proteasome and autophagy‒lysosome pathways, thus suppressing downstream DNA repair enzymes (SMUG1 and LIG4). This dual mechanism combines direct genotoxicity with impaired DNA repair capacity, underlying the potent anti-GBM efficacy. Our platelet-based FL delivery represents a practical, safe, translational therapy for overcoming BBB limitations by disabling HIF-1α-regulated DNA repair.
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ANALYTICAL LETTERS
·
2026
·
IF 1.8
Panpan You,Lei Li,Wenhui Yang,Shuxin Liang,Taiqun Yang,Chaoqun Ma,Guoqing Chen,Chun Zhu,Hui Gao
The effective treatment of iodine-containing wastewater is essential for environmental protection, as elevated iodide concentrations can adversely affect ecosystems and human health. Traditional methods for iodide monitoring and pH adjustment often involve time consuming multi step procedures, limiting their efficiency in real time wastewater management. In this study, we introduce a deep learning assisted analytical strategy that enables the simultaneous determination of iodide concentration and pH from a single fluorescence lifetime decay curve. The approach is based on the dual sensitivity of fluorescein sodium, which exhibits distinct fluorescence responses in different environments: protonation occurs under acidic conditions, deprotonation under alkaline conditions, and dynamic fluorescence quenching is triggered by iodides. By integrating time correlated single-photon counting (TCSPC) with a hybrid convolutional neural network–residual network–random forest regression (CNN-ResNet-RF) model, we achieve rapid and accurate prediction of both parameters without chemical separation or additional reagents. The proposed model not only overcomes the nonlinear coupling between pH and iodide effects on fluorescence lifetime but also demonstrates strong generalization performance in simulated wastewater matrices. This work provides a robust, reagent-free, and high-throughput analytical platform for real-time monitoring and process control in iodine-containing wastewater treatment.
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MOLECULES
·
2026
·Vol. 31
·期 4
·671
·
IF 5.1
Shumeng Liu,Yue Xing,Yue Na,Hao Wu,Chi Liu,Zhigang Wang,Ning Zhang,Xiuhong Wu,Fang Geng
Luteolin (Ltn), a natural flavonoid, effectively inhibits microglial activation in Alzheimer’s disease (AD) with promising therapeutic potential, but its efficacy is severely limited by the blood–brain barrier (BBB). To overcome this obstacle, this study prepared poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs)—designated as TGN/RAP12-RBC-NPs@Ltn—which were coated with red blood cell membranes (RBCm) functionalized with two peptides, TGN (TGNYKALHPHN) and RAP12 (EAKIEKHNHYQK). The results demonstrated that TGN significantly enhanced BBB permeability, while RAP12 enabled effective targeting and delivery of TGN/RAP12-RBC-NPs@Ltn to microglial mitochondria in the brain. In addition, the presence of RBCm significantly inhibited the phagocytosis of NPs by macrophages, exerting a notable role in immune evasion. Meanwhile, the study confirmed that encapsulating Ltn within NPs significantly enhanced cognitive function in APP/PS1 mice, modulated the expression of key mitochondrial metabolic enzymes—pyruvate dehydrogenase (PDH) and its phosphorylated forms (pS232PDH, pS293PDH, pS300PDH)—in microglia, thereby ameliorating mitochondrial dysfunction and effectively regulating the neuroinflammatory environment in the mouse brain, and ultimately contributed to therapeutic efficacy. From this, it could be seen that TGN/RAP12-RBC-NPs@Ltn could significantly enhance the therapeutic effect of Ltn on AD, providing an effective treatment strategy for delaying the progression of AD.
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ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Jingtong Sun,Xintao Jia,Jing Xue,Xiuping Deng,Changxiang Yu,Yiqing Kou,Xinru Shen,Mengru Yang,Ying Zhang,Zhidong Liu
Oral administration is the most common and patient friendly route for drug delivery. However, the harsh gastric environment severely compromises the stability and absorption of many therapeutics, particularly macromolecules. Here, we report an oral microrobot (OMR), inspired by a tumbler toy, designed for safe and efficient gastric drug delivery. The OMR incorporates interchangeable modules for either liquid drug injection or delivery of poorly soluble drugs via microneedles. Natural polysaccharides enhance adhesion to the gastric mucosa, dissolvable threads activate a spring-driven injection mechanism, and effervescent tablets enable controlled device detachment. In vivo studies in beagle dogs showed that, after oral administration, the protective shell dissolved under gastric conditions, triggering drug release. The macromolecular substance insulin, with its direct regulatory effect on blood glucose levels, has been selected as a typical model drug. Insulin delivered by the OMR injection module achieved a hypoglycemic effect comparable to subcutaneous injection. The OMR was excreted intact within 12 h without causing gastrointestinal injury. These findings demonstrate the potential of OMR system as a robust platform for delivering biologics otherwise inactivated in the gastrointestinal tract.
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Food Bioscience
·
2026
·
IF 6.2
You Li,Qiyang Huang,Jianquan Kan,Antonio Pérez-Gálvez,Saied A. Aboushanab,Krisztina Takács,Leila Queiroz Zepka,Tian Cai,Kewei Chen
Idesia polycarpa var. vestita is a promising woody fruit resource rich in bioactive phenolic compounds and carotenoids. However, the influence of geographic origin on its phytochemical composition and antioxidant potential remains insufficiently understood. To explore the geographic variation in fruit quality, fruits collected from six regions were compared in terms of phenotypic traits, phytochemical composition, and in vitro antioxidant capacity. Metabolite profiling annotated 33 phenolic compounds and 37 carotenoids. Salicinoid-type glycosides dominated the phenolic fraction, while the carotenoid profile was characterized by abundant lutein esters, particularly lutein diesters. Their accumulation differed considerably among geographic origins, accompanied by variation in antioxidant activity. Total phenolic content ranged from 8.60 to 16.20 mg GAE/g DW and was positively correlated with DPPH, ABTS, and FRAP activities. Total carotenoid content varied from 156.0 to 577.0 μg β-carotene equivalents/g DW and showed a positive correlation with ORAC activity. Beyond these total-content indices, variation in antioxidant potential was more closely associated with the abundance of specific metabolites. Salicinoids and related phenolic glycosides, together with lutein diesters, were among the characteristic metabolites associated with regional differences in antioxidant potential. Geographic differentiation of I. polycarpa var. vestita fruits thus primarily reflected shifts in the abundance of dominant phenolic and carotenoid metabolites rather than major changes in metabolite classes. These results link geographic origin with phytochemical variation and antioxidant properties and provide a basis for further evaluation of I. polycarpa var. vestita as a carotenoid- and phenolic-rich food resource.
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PEST MANAGEMENT SCIENCE
·
2026
·
IF 4.2
Rui Liu,Yufei Ma,Yue Zhao,Ye Liu,Zhenglan Du,Longlong Li,Chenhui Zhang,Fengpei Du
BACKGROUND Urea is frequently tank-mixed with pesticides, yet whether it functions as a conventional spray adjuvant or acts at another stage of droplet evolution remains unclear. This study examined how 0–2.0 wt% urea affects spray liquid properties, atomization, impact, wetting, evaporation, and final deposition. RESULTS Urea caused only minor changes in equilibrium surface tension and viscosity. Across nozzle and pressure conditions, changes in volume median diameter were generally within ±3%, while impact, wetting, and the time required for 90% volume loss (55–60 min) were not appreciably altered. In contrast, dried residue morphology changed markedly. In the sodium-fluorescein-containing 32% thifluzamide–tebuconazole suspension concentrate system on polytetrafluoroethylene (PTFE), which contained sodium fluorescein, the addition of 0.5 wt% urea increased the central integrated fluorescence fraction from 21.90% to 26.40% (+4.50 percentage points) and the center-to-periphery ratio from 2.24 to 2.87. Similar central enrichment occurred at 1.0–1.5 wt%, whereas 2.0 wt% reduced the fraction to 20.66%. More continuous residues were also observed qualitatively on maize and rice leaves. CONCLUSION Urea primarily modifies deposition during late-stage droplet drying rather than through atomization or wetting. The observed redistribution is consistent with evaporation-driven solute enrichment, precipitation, and crystallization. These findings identify 0.5 wt% as the lowest tested concentration producing high central enrichment in the examined formulation and establish late-stage drying as a distinct target for improving pesticide deposit spatial distribution, although biological efficacy requires further verification. © 2026 Society of Chemical Industry.
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NEUROCHEMICAL RESEARCH
·
2026
·Vol. 51
·期 5
·257
·
IF 4.5
Cao Zhi,Yin Linting,Zhang Xinyong,Hong Enhui,Du Jiansheng,Liang Rui,Tang Youjia,Zhang Guofeng
Spontaneous cerebral hemorrhage (sICH) is one of the cerebrovascular diseases with the highest mortality rate. MicroRNAs (miRNAs) are implicated in the regulation of various pathological processes, however, the specific function of miR-642b-3p in sICH remains unclear. Serum miR-642b-3p levels of sICH patients was determined. A thrombin-induced human brain microvascular endothelial cell (hBMECs) injury model was established. Cell viability, apoptosis, inflammatory factors, and related protein expression were assessed using CCK-8 assay, flow cytometry, ELISA, and Western blot. The targeting relationship between miR-642b-3p and CCNA2 was validated via a dual-luciferase reporter assay. MiR-642b-3p in the serum of ICH patients was significantly increased. Following thrombin induction, miR-642b-3p was upregulated in hBMECs, accompanied by decreased cell viability, increased apoptosis, elevated production of inflammatory factors, upregulated expression of pro-apoptotic proteins (Bax and cleaved caspase-3), and downregulated expression of anti-apoptotic protein (Bcl-2). Additionally, the protein levels of tight junction markers ZO-1 and occludin were markedly decreased. Notably, inhibition of miR-642b-3p significantly reversed these thrombin-induced changes and alleviated cell damage. Mechanistically, CCNA2was confirmed as a direct downstream target gene of miR-642b-3p. MiR-642b-3p is upregulated in sICH and mediates thrombin-induced endothelial cell injury, at least in part, by targeting CCNA2. These findings suggest that the miR-642b-3p/CCNA2 axis may represent a potential mechanistic pathway involved in ICH-induced blood-brain barrier disruption.
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危险品化学品经营许可证（带存储）