SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2013
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IF 1.977
Jiangang Zhang,Li Zhang,Yanli Wei,Jun Ma,Shaomin Shuang,Zongwei Cai,Chuan Dong
A novel fluorescein derivative furfuraldehyde fluorescein hydrazone (FFH) has been synthesized by reacting fluorescein hydrazide with furfuraldehyde and characterized by 1 H NMR, 13 C NMR, MS and elemental analysis. Addition of Cu 2+ to the solution of FFH results in a rapid color change from colorless to yellow together with an obvious new band appeared at 502 nm in the UV–vis absorption spectra. This change is attributed to the spirocycle form of FFH opened via coordination with Cu 2+ in a 1:1 stoichiometry and their association constant is determined as 6.1 × 10 4 L mol −1 . Experimental results indicate that the FFH can provide a rapid, selective and sensitive response to Cu 2+ with a linear dynamic range 6.6–330 μmol/L. Common interferent ions do not show any interference on the Cu 2+ determination. It is anticipated that FFH can be a good candidate probe and has potential application for Cu 2+ determination in aqueous solution.
研究领域:
分析化学
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环境科学
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材料科学
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DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Yang Zhou,Jianxiu Du,Zhen Wang
Fluorescein and its derivatives, including 4′,5′–dibromofluorescein, 2′,7′–dichlorofluorescein and 2′,4′,5′,7′–tetrabromofluorescein can be employed as efficient coreactants for luminol reaction. Under an alkaline condition they reacted with luminol to produce measureable chemiluminescence (CL) in the absence of any extra–oxidant. Cobalt ion (Co 2+ ) significantly enhanced the CL emission of the reaction. The CL emission was strongly dependent on the concentration of carbonate buffer used; higher concentration of carbonate buffer provided more intense CL emission. The system had two emission peaks locating at 425 nm and 535 nm, respectively. With 4′,5′–dibromofluorescein as the coreactant for luminol reaction, a new CL method was developed for the detection of Co 2+ . The procedure allowed the linear detection of Co 2+ in the range of 5–1000 nmol/L. The limit of detection (LOD) and limit of quantitation (LOQ) were 1.8 nmol/L and 5.0 nmol/L, respectively. The intra–day precision (n = 11) and inter–day precision (n = 3) was 3.2% and 4.7% for replicated measurements of 0.2 μmol/L Co 2+ solution. The method was successfully applied to the determination of Co 2+ in blue silica gel samples.
研究领域:
分析化学
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环境科学
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材料科学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2017
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IF 6.32
Huateng Zhang,Jing Su,Yuxin Lin,Haixiu Bai,Jiaxiang Liu,Hui Chen,Lupei Du,Lichuan Gu,Minyong Li
Chalcone refers to an aromatic ketone and an enone that constitutes the central core for various important biological compounds in drug discovery. Moreover, the firefly luciferase (Fluc) as the bioluminescent reporter has been widely used in life science research and high-throughput screening (HTS). However, Fluc might suffer from direct inhibition by HTS compounds resulting in the occurrence of “false positives.” In the current research, we discovered a series of chalcone compounds as Fluc inhibitors with favorable potency both in vitro and in vivo. Moreover, our compound 3i showed remarkable systemic inhibition in transgenic mice. Both enzymatic kinetics study and cocrystal structure demonstrated that compound 3i is competitive for substrate aminoluciferin, while noncompetitive for ATP. Besides, compound 3i exhibited excellent selectivity as a promising quenching agent in a simulated dual-luciferase reporter assay. We believed that our research would contribute to improving scientists’ awareness of the Fluc inhibitors, pay attention to the bias results, and even expand the utilization of bioluminescence in life science research.
研究领域:
药理学
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分子生物学
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生命科学
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DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 64
·59677-59683
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IF 3.289
Xin Wu,Xiaojuan Gong,Wenjuan Dong,Jun Ma,Jianbin Chao,Chenzhong Li,Li Wang,Chuan Dong
A new colorimetric probe, 5-chlorosalicylaldehyde fluorescein hydrazone (CSFH), has been synthesized and characterized by infrared spectroscopy, nuclear magnetic resonance spectroscopy, mass spectrometry, elemental analysis and X-ray single crystal diffraction. CSFH had nearly no absorption in visible region. In the presence of copper(II) ion a rapid color changes from colorless to yellow along with an obvious new absorption band appeared at 495 nm. CSFH was highly selective to Cu2+ only in the aqueous solution, instead of common metal ions. The absorbance intensity and the color of CSFH solution increased gradually with the increase of Cu2+ concentration. Common metal ions did not show any interference on the determination of Cu2+. The chemical stoichiometric ratio between the CSFH and Cu2+ was 1 : 1 and corresponding association constant was 6.85 × 105 L mol−1, and the linear range for Cu2+ detection was 0.25–14 μmol L−1. The detection limits were 0.25 μmol L−1 and 1.0 μmol L−1 of Cu2+ using the UV-Vis changes and the visual color changes by the naked eye respectively. The proposed method was applied to the determination of Cu2+ in drinking water samples and the recoveries were 96–106%. The preparation of CSFH exhibited the quick, simple and facile advantages. The results showed that CSFH can be a good candidate for simple, rapid and sensitive colorimetric detection of Cu2+ in aqueous solution.
研究领域:
分析化学
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环境科学
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产品关联
DOI
高影响因子
MATERIALS LETTERS
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2018
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IF 2.687
Qifeng Liu,Bingrui Liu,Qian Zhang,Jianping Gao,Jingjun Ma
High-efficiency hydrogen evolution is obtained from a novel fluorescein homogeneous system, comprising a noble metal free fluorescein as the photosensitizer, Pt nanoparticle as the cocatalyst and TEOA as the holes scavenger in an aqueous solution under visible light irradiation. The highest H 2 production rate is 491.40 μmol/h (λ > 420 nm) and quantum efficiencies of our system are among the highest reported for the molecular hydrogen systems in the visible light range. Unlike many reported molecular hydrogen systems are either unstable under illumination and expensive, or have weak photocatalytic performances, our low-cost system exhibits excellent activity and endurance. We believe that this low-cost, high-efficiency and durable homogeneous system for molecular hydrogen may result in new breakthroughs for the hydrogen energy and solar energy utilization.
研究领域:
能源科学
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光化学
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材料科学
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产品关联
DOI
高影响因子
Biosensors-Basel
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2023
·Vol. 13
·期 3
·301
·
IF 5.4
Yinjuan Bai,Hongpeng Zhang,Bingqin Yang,Xin Leng
Copper is a common metallic element that plays an extremely essential role in the physiological activities of living organisms. The slightest change in copper levels in the human body can trigger various diseases. Therefore, it is important to accurately and efficiently monitor copper ion levels in the human body. Recent studies have shown that fluorescent probes have obvious advantages in bioimaging and Cu2+detection. Therefore, a novel Cu2+probe (N2) was designed and synthesized from fluorescein, hydrazine hydrate and 5-p-nitrophenylfurfural that is sensitive to and can detect Cu2+within 100 s. The response mechanism of the N2 probe to Cu2+was studied by several methods such as Job’s plots and MS analysis, which showed that the Cu2+and the N2 probe were coordinated in a complexation ratio of 1:1. In addition, compared with other cations investigated in this study, the N2 probe showed excellent selectivity and sensitivity to Cu2+, exhibiting distinct fluorescence absorption at 525 nm. Furthermore, in the equivalent range of 0.1–1.5, there is a good linear relationship between Cu2+concentration and fluorescence intensity, and the detection limit is 0.10 μM. It is worth mentioning that the reversible reaction between the N2 probe and Cu2+, as well as the good biocompatibility shown by the probe in bioimaging, make it a promising candidate for Cu2+biosensor applications.Keywords:fluorescein-based probe;complexation mechanism;copper ion detection;bioimaging
研究领域:
生物化学
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分析化学
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细胞生物学
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DOI
PubMed
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
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2019
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IF 3.261
Jinxia Xu,Fanyong Yan,Xiaodong Sun,Zhaoxu Wang
Two Schiff bases fluorescence sensors (FP 1 and FP 2 ) based on fluorescein-monoaldehyde derivatives were designed, synthesized and characterized. The effect of electron-donating and electron-withdrawing substituents on fluorescence of fluorescein was investigated. Through the calculation of the frontier orbital, it is proved that when the probe coexists with Cu 2+ , the complex system is more stable and easier to form. The FP 1 was used as effective fluorescent contrast agent for determining Cu 2+ from 0 to 55 μM with the limit of detection of 0.23 μM in several real water samples, sensing membranes and intracellular imaging.
研究领域:
分析化学
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生物化学
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产品关联
DOI
高影响因子
RSC Advances
·
2013
·Vol. 3
·期 42
·19403-19408
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IF 2.562
Juan Wei,Shengyang Yang,Lifang Wang,Cai-Feng Wang,Li Chen,Su Chen
We report the fabrication of composite nanofiber mats of poly(lactic acid)/poly(glycidyl methacrylate)/fluorescein [(PLA)/(PGMA)/fluorescein] hybrids via an electrospinning route. Initially, PGMA with low molecular weight was synthesized by free radical polymerization. Then the fluorescent electrospun nanofiber mats were fabricated from the blending of fluorescein and PLA/PGMA in methylene chloride/N,N′-dimethylformamide solution. The influences of the reagent proportions and electrospinning parameters on the morphology of the nanofibers were thoroughly investigated. The as-prepared electrospun fibrous mats showed favorable fluorescence, which meant that the mats could be used in fingerprint recognition and silk-screen printing, as well as amine analysis. This facile method may find potential applications in forensic investigation, reactive microchips and medical diagnostics.
研究领域:
材料科学
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法医学
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生物医学工程
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产品关联
DOI
高影响因子
LAB ON A CHIP
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2021
·Vol. 21
·期 11
·2217-2222
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IF 6.799
Lingjun Zhang,Jianfeng Sun,Hong He,Yingzhou Huang,Haiyang Shi,Weigen Chen
The Raman detection of trace substances in complex oil is still a great challenge at present because of the strong disturbance of background activity and the suppression of intensity in spectra caused by complicated components. In this work, a simple and robust approach based on microdroplet liquid–liquid extraction for the real-time Raman spectroscopy monitoring of trace substances in complex oil is reported. Based on unbalanced chemical potentials between water and oil on a microfluidic chip, a target trace molecule is extracted from complex mineral oil to a water microdroplet. Benefiting from the real-time fluorescence intensities of fluorescein in a water microdroplet, the extraction performance is investigated and optimized. The optimal water microdroplet is implemented for the Raman detection of furfural in a complex mineral oil, a typical trace performance marker in electric power equipment, and this exhibits excellent sensitivity with a limit of detection (LOD) of 26 ppb. Compared to traditional detection technology for trace substances in complex oil (high performance liquid chromatography, HPLC), this method greatly simplified the process of measurement, reduced the volume of sample required, had a fast measurement time, and exhibited the prospect of real-time monitoring applications with high sensitivity, which not only promotes the development of oil quality but also enlarges existing knowledge related to using Raman spectroscopy in chem−/bio-sensing.
研究领域:
分析化学
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光谱学
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材料科学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2023
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IF 8.4
Weiyi Lu,Shuyan Lou,Bin Yang,Meixuan Wu,Huimin Hao,Zihua Guo
The convenient monitoring of carbaryl is necessary because of its serious threat to environment and public health. In enzyme inhibition-based colorimetric detection, the oxidation of colorimetric substrate (3,3′,5,5′-tetramethyl benzidine) only proceeds at pH below 5, while most enzymes only work efficiently at neutral pH. Here fluorescein was doped in coordination polymers to serve as novel nanozymes for efficient light-activated oxidation. The long triplet state lifetime of Ce 3+ made the substrate oxidize at neutral pH to overcome a pH limitation. Furthermore, one interesting light-activated biosensing system was developed based on the carbaryl’s unique toxicity towards acetylcholinesterase. It can sensitively detect carbaryl at neutral pH with a limit of detection 1.53 μg/L, which is much lower than the maximum residue limit (50 μg/L) in European Union database. It also enjoys a good selectivity for carbaryl detection over other pesticides or endocrine disruptors . Besides, the biosensing system showed a satisfied performance to detect carbaryl in both water and food samples.
研究领域:
分析化学
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环境科学
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材料科学
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生物传感器
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产品关联
DOI
高影响因子
JOURNAL OF ANALYTICAL CHEMISTRY
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2020
·Vol. 75
·期 3
·330-342
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IF 0.84
Haishuang Jia,Liu Wenfang,Li Na,Wang Jun,Song Youtao
Abstract Two novel methods for spectrophotometric determination of Cu 2+ ion using synthesized and characterized furfuraldehyde fluorescein diacetate hydrazone ( FFDH ) and furfuraldehyde fluorescein hydrazone ( FFH ) have been proposed. Addition of Cu 2+ to FFDH and FFH solutions results in a rapid color change from colorless to dark yellow. Experimental results show that FFH and FFDH both can provide a rapid, selective and sensitive response to Cu 2+ within a linear dynamic range of 7–33 µM and the detection limits of 1.1 × 10 –7 and 7 × 10 –8 M, respectively. Apparently, the detection limit of FFDH is lower than that of FFH, which indicates that FFDH forms a more stable complex with Cu 2+ . In addition, the detection of Cu 2+ in water samples was also studied; as a result, the developed FFDH was found to be more applicable than FFH for spectrophotometric determination of Cu 2+ in environmental water samples. Particularly, as a lipid chemical compound, FFDH may be transported across the cell membrane to detect Cu 2+ in vivo. It is of great significance in many fields, such as biology, biochemistry, medicine and environment.
研究领域:
分析化学
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环境科学
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生物化学
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DOI
高影响因子
Canadian Journal of Chemistry
·
2018
·Vol. 96
·期 12
·
IF 1.084
Guanhong Liu,Peipei Ren,Fan Yang,Xuekai Dou,Jun Wang,Youtao Song
Two novel isomeric colorimetric probes are established for simultaneous determination of copper ions using 2-hydroxy-5-methoxybenzaldehyde fluorescein hydrazone (5-HMBA-FH) and 2-hydroxy-3-methoxybenzaldehyde fluorescein hydrazone (3-HMBA-FH). They are synthesized by reacting fluorescein hydrazide with 2-hydroxy-5-methoxybenzaldehyde and 2-hydroxy-3-methoxybenzaldehyde, respectively, and then characterized by 1H-NMR, 13C-NMR, and infrared spectrum. The addition of copper ions to the solutions of two novel colorimetric probes can generate the obviously peaks at 498 nm in UV–vis absorption spectra along with a rapid colour change from colourless to dark yellow. The detection limits of the method for Cu2+ ion were 3.442 × 10−6 mol/L and 3.682 × 10−6 mol/L separately for 5-HMBA-FH and 3-HMBA-FH, respectively. The additions of other metal ions hardly affect the determination of copper ions. The proposed method was successfully applied to the analysis of Cu2+ ions in various samples. This method possesses high sensitivity, high simplicity, and minimized interference and will provide a great advantage in detecting copper ions in the environment, food, and medical applications.
研究领域:
分析化学
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环境科学
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医学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）