FOOD CONTROL
·
2022
·
IF 6.652
Sihan Zhang,Qinzhen Fan,Jiaqi Guo,Xianhe Jiao,Xianming Kong,Qian Yu
Sensitive and rapid detection of histamine in fish sample was important for human health. A novel and facile method for on-site detecting histamine in fish was developed by tandem surface-enhanced Raman spectroscopy (SERS) with derivatized thin-layer chromatography. Histamine in tuna was firstly separated by TLC process on a diatomite plate, and then derivatized with fluorescamine (FSA). SERS is used for identification of histamine derivative by a portable Raman spectrometer. The operating conditions were optimized for improving the detection performance. Diatomite plate was suitable for separation and derivatization, 40 times concentrated Au colloid, 1 mM of FSA at pH = 8 were appropriate for the derivatization and SERS detection, and the limit of detection of histamine could achieve 9 ppb. The results indicated that the proposed method not only visualized the histamine on TLC plate but also improve the sensitivity in SERS, that could be employed for the on-site separating and detecting histamine from fish sample.
研究领域:
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
LANGMUIR
·
2022
·
IF 4.331
Yiping Chen,Zhenze Lou,Zihao Chen,Shuangye Yang,Jiashuo Hu,Jie Wei
In this study, we fabricate magnetic–fluorescent responsive Janus photonic crystal beads (JPCBs) based on poly(styrene-methyl methacrylate-acrylic acid) (p(St-MMA-AA)) colloidal nanoparticles, Fe3O4, and photobase generators used for self-destructive anti-counterfeiting. We synthesize two kinds of photobase generators that can react with fluorescamine to produce various fluorescence colors. A microfluidic method is used to obtain the Janus photonic crystal beads. The upper portions of the JPCBs are photonic crystals assembled with colloidal spheres, whereas the Fe3O4 settles down to the bottom of the JPCBs due to its higher density. Photobase generators are distributed in photonic crystal gaps. Because of the magnetism of the Fe3O4, the JPCBs could be flipped from one side to the other in the presence of a magnet. After being exposed to UVC light and fluorescamine, the JPCBs can fluoresce under UVA light. Then, we create Janus microbeads arrays with various types of beads and apply them to the visitor card, bracelet, and box label to provide irreversible and self-destructive anti-counterfeiting. The JPCBs are capable of being encoded and angle-independently displayed, which are crucial to their applications in anti-counterfeiting, information coding, and array display.
研究领域:
材料科学
·
纳米技术
·
防伪技术
·
光学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2025
·
IF 5.5
Pu Chen,Zhe-Wen Lu,Hai-Bo Mao,Hong-Bo Wang,Jing-Bo Hu,Jing Li
Sepsis-induced cardiomyopathy (SCM) is a severe complication characterized by acute myocardial dysfunction and reduced cardiac output, often leading to high mortality despite advances in critical care. Central to SCM progression is the dysregulation of inflammatory and oxidative stress pathways. Ferroptosis, a form of iron-dependent cell death, plays a crucial role in SCM pathogenesis. Bardoxolone methyl (BM) has shown potent antiferroptotic activity through dual modulation of oxidative stress and inflammation. However, its clinical application is limited by nonspecific tissue distribution and low bioavailability. Here, we show that a myocardial-targeting peptide-conjugated nanoplatform, PCM-modified liposomes (PCM-Lipo/BM), significantly enhances the targeted delivery of BM to myocardial cells. PCM-Lipo/BM treatment markedly increased BM uptake in myocardial cells, alleviated lipopolysaccharide (LPS)-induced myocardial injury, improved cardiac function metrics, and reduced inflammation and oxidative stress markers. Compared to BM or Lipo/BM alone, PCM-Lipo/BM exhibited superior efficacy in reducing TNF-α, IL-6, and IL-1β levels. These findings demonstrate that PCM-Lipo/BM effectively mitigates inflammatory responses and oxidative damage in myocardial cells, offering a promising precision medicine approach to SCM treatment. This study not only enhances our understanding of SCM pathogenesis but also proposes a therapeutic strategy that could be applied to other cardiovascular diseases characterized by similar pathological mechanisms.
研究领域:
心血管医学
·
纳米医学
·
药理学
·
炎症性疾病
·
氧化应激与细胞死亡
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2026
·
IF 4.6
Feiran Xu,Tong Ji,Yu Wang,Guosheng Xu,Xingguang Chen,Zhaoming Wang,Bao Zhang,Jiansheng Zhao,Baocai Xu
γ-Glutamyl dipeptides (γ-GGDPs) have been implicated be generated by enzymatic action on γ‑glutamyl groups, modulate kokumi sensation of fermented foods via activating calcium-sensing receptors (CaSR). However, their variation and potential formation mechanism in Jinhua ham remain unclear. 10 γ-GGDPs (γ-EA, γ-ED, γ-EE, γ-EL, γ-EM, γ-EG, γ-EF, γ-EY, γ-EI, γ-EW) in Jinhua ham were quantified by UHPLC-Q-TOF-MS and accumulating significantly during processing ( P < 0.05), with final contents ranging from 0.78 ± 0.01 μg/g (γ-EW) to 24.75 ± 0.33 μg/g (γ-EA). Sensory evaluation confirmed these γ-GGDPs enhanced the kokumi, saltiness, and umami of Jinhua ham during the post-ripening periods. Mechanistic investigation indicated that γ-glutamyl transpeptidase (GGT) transfer and hydrolytic activities increased progressively to 9.11 ± 0.17 U/mg and 12.19 ± 0.40 U/mg, respectively, which corroborated GGT's role in driving γ-GGDPs formation by catalyzing the transfer of γ-glutamyl groups to free amino acids (FAAs) generated by endogenous enzymes in Jinhua ham. Further validation via a ternary reaction system (comprising FAAs, γ-glutamyl donors, and GGT extracted from Jinhua ham) confirmed this GGT-catalyzed transfer reaction for γ-GGDPs biosynthesis. This study provides a theoretical foundation for the potential formation mechanisms of γ-GGDPs in Jinhua ham and regulation of Kokumi sensation in fermented foods.
✓ 已验证
产品关联
DOI
高影响因子
Materials Today Bio
·
2026
·
IF 11
Youming Xu,Yuxin Zhang,Jiyuan Chen,Zihan Zhuang,Chen Zhang,Kaixuan Ren,Dong Chen,Jingbo Yin
Guided bone regeneration (GBR) membranes play a critical role in repairing bone defects, yet conventional collagen-based membranes suffer from immunogenicity, insufficient mechanical strength, and lack of osteoinductivity. Herein, a multi-biomimetic poly(γ-benzyl-L-glutamate) (PBLG)-based Janus membrane (PP-S/MH-P) is constructed that integrates a robust barrier function with potent osteoinductive capacity. The membrane is fabricated with a Janus architecture: PP-S side consists of a dense poly(γ-benzyl-L-glutamate) (PBLG) barrier layer, physically blended with PEGDA and surface-grafted with zwitterionic poly(sulfobetaine methacrylate) (PSBMA) brushes, the opposite MH-P side comprises Mg-doped hydroxyapatite surface-grafted with PBLG, forming a bone-mimetic nanofibrous network. The membrane exhibits robust mechanical properties (tensile strength: 5.42 MPa; elastic modulus: 141.72 MPa), favorable wet tissue conformability, and enzymatic degradation. The PP-S side effectively resists bacterial ( E. coli and S. aureus ) invasion and fibroblast adhesion; while the MH-P side enables sustained Mg 2+ release, significantly promotes rBMSCs spreading, mineralization, and upregulation of osteogenic genes (RUNX2, ALPL, OCN). In a rat calvarial defect model, PP-S/MH-P membrane prevents soft-tissue infiltration and achieves outstanding new bone formation, with BV/TV reaching 86.13 ± 7.61% at 12 weeks. This multi-biomimetic Janus membrane, combining compositional, structural, and functional biomimicry, offers a promising strategy for designing high-performance GBR membranes with simultaneous barrier protection and osteogenic promotion.
✓ 已验证
产品关联
DOI
高影响因子
Biomaterials Advances
·
2025
·
IF 6
Ziyuan Guo,Zongliang Wang,Peibiao Zhang
Although biological adhesives have shown advantages in replacing traditional wound suturing techniques, there are still limitations in wound closure, hemostasis, and healing, including insufficient tissue adhesion, potential biological toxicity, and complex preparation processes. In this study, a facile route for preparing injectable dual-crosslinking multifunctional hydrogel adhesive (Gel/EN/FBTA) was developed. The Gel/EN/FBTA adhesive is a dynamic cross-linked network composed of tannic acid (TA), 3-formylphenylboronic acid (3-FPBA) and gelatin, which can provide a large number of bonding sites and strengthen the adhesive cohesion through energy dissipation. The amidation reaction inside gelatin can form stable rigid crosslinks and maintain the structure of the adhesive stably. The balance between adhesion and cohesion can be regulated by adjusting the chemical composition and crosslinking density of the dual-crosslinking network. Under this equilibrium condition, the adhesion strength of Gel/EN/FBTA2 hydrogel is 3 times that of commercial fibrin glue, which shows good hemostatic effects in rat liver injury, rat tail injury, and rabbit liver cross incision models. Furthermore, Gel/EN/FBTA2 hydrogel adhesive can effectively treat wound infection, reduce inflammation level, promote re-epithelialization, accelerate collagen deposition, and achieve the healing of infectious full-thickness wounds. This dual-network design paradigm provides a versatile strategy for developing next-generation bioadhesives with tailored mechanical and bioactive properties, demonstrating significant potential for non-compressible hemorrhage and infected wound management.
研究领域:
生物材料学
·
医学
·
组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2025
·
IF 16
Xianyao Wu,Lanjing Wang,Guoshuai Zhang,Lin Ma,Dawei Wang,Yafei Zhang,Yuxin Bao,Xiaojia Wu,Xiang Li,Yufeng Hu,Hong-shang Peng
Silica coating is widely employed to improve the stability of perovskite quantum dots (PQDs) as light conversion phosphors. When used in combination with micro-light-emitting diodes (micro-LEDs) for next-generation displays, however, most silica-coated PQDs (Si-PQDs) face some challenges: (i) polarity mismatch between silica and organic solvent undermines the fabrication of the phosphor film by unstable PQD ink; (ii) aggregation-induced large particle size hinders the micron-scale processability. Herein, we report a ligand-assisted silica-coating strategy to prepare highly monodispersed Si-PQDs for micro-LEDs. Briefly, pristine CsPbBr3 QDs capped with aminosiloxane were first formed followed by surface passivation with the zwitterionic ligand lecithin and subsequent tetramethoxysilane-mediated silica coating. The as-prepared Si-PQDs were highly monodispersed with well-defined core–shell structure, exhibiting uniform rectangular morphology, narrow-band green emission, ultrahigh photoluminescence quantum yield (∼98%), and high stability against light and water. Importantly, the Si-PQDs were well dispersed in organic solvents, which could not only be fabricated into white LEDs and screen-printed patterns but also patterned into uniform 20 μm pixels using a microfluidic technique. These results demonstrate that the one-pot synthesized Si-PQDs are very promising light conversion phosphors for micro-LED-based displays and solid-state lighting.
研究领域:
纳米技术
·
材料科学
·
光电子学
·
显示技术
·
半导体器件
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Tingwu Liu,Shunjie Yan,Rongtao Zhou,Xu Zhang,Huawei Yang,Qiuyan Yan,Ran Yang,Shifang Luan
Surface-tethered hierarchical polymer brushes find wide applications in the development of antibacterial surfaces due to the well-defined spatial distribution and the separate but complementary properties of different blocks. Existing methods to achieve such polymer brushes mainly focused on inorganic material substrates, precluding their practical applications on common medical devices. In this work, a hierarchical polymer brush system is proposed and facilely constructed on polymeric substrates via light living graft polymerization. The polymer brush system with micrometer-scale thickness exhibits a unique hierarchical architecture consisting of a poly(hydroxyethyl methacrylate) (PHEMA) outer layer and an anionic inner layer loading with cationic antimicrobial peptide (AMP) via electrostatic attraction. The surface of this system inhibits the initial adhesion of bacteria by the PHEMA hydration outer layer under neutral pH conditions; when bacteria adhere and proliferate on this surface, the bacterially induced acidification triggers the cleavage of labile amide bonds within the inner layer to expose the positively charged amines and vigorously release melittin (MLT), allowing the surface to timely kill the adhering bacteria. The hierarchical surface employs multiple antibacterial mechanisms to combat bacterial infection and shows high sensitiveness and responsiveness to pathogens. A new paradigm is supplied by this modular hierarchical polymer brushes system for the progress of intelligent surfaces on universal polymer substrates, showing great potential to a promising strategy for preventing infection related to medical devices.
研究领域:
材料科学
·
生物医学工程
·
高分子化学
·
抗菌技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 5
·1020
·
IF 4.2
Jing Ren,Lin Li,Hongfei Han,Yi Chen,Ziying Qin,Zhen Song
Biomacromolecular probes have been extensively employed in the detection of metal ions for their prominent biocompatibility, water solubility, high selectivity, and easy modification of fluorescent groups. In this study, a fluorescent probe FP was constructed. The probe FP exhibited high specificity recognition for Cu2+. With the combination of Cu2+, the probe was subjected to fluorescence quenching. The research suggested that the probe FP carried out the highly sensitive detection of Cu2+with detection limits of 1.7 nM. The fluorescence quenching of fluorescamine was induced by Cu2+perhaps due to the PET (photoinduced electron transfer) mechanism. The FP-Cu2+complex shows weak fluorescence, which is likely due to the PET quenching effect from Cu2+to fluorescamine fluorophore. Moreover, the probe FP can be employed for imaging Cu2+in living cells. The new fluorescent probe developed in this study shows the advantages of good biocompatibility and low cytotoxicity. It can be adopted for the targeted detection of Cu2+in cells, and it has promising applications in the mechanism research and diagnosis of Cu2+-associated diseases.
研究领域:
生物化学
·
分子生物学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Jing Li,Xiang-Dong Xu,Jian Zhang,Xin-Yi Sui,Jian-Bo Qu
Macroporous polystyrene microspheres have been extensively used as an efficient carrier for lipase immobilization owing to the hydrophobic surface , excellent mechanical strength and chemical stability, easy to recycle and low cost. However, conventional polystyrene microspheres usually have a pore size of less than 100 nm and an extremely hydrophobic surface lacking functional groups, which bring a series of problems such as low enzyme loading, activity recovery, reusability, serious leakage, and high substrate diffusion limitation. In this work, a novel generation of gigaporous polystyrene microspheres with moderately hydrophilic surfaces was specifically designed as an enzymatic carrier. The microspheres were featured with micropores (∼ 9 nm), mesopores (∼ 54 nm), and gigapores (∼ 948 nm), ensuring native enzyme structure preservation and high loading capacity. After epoxy activation, the lipase PS from Burkholderia cepacia (PPL) was site-specifically immobilized onto the microspheres. The resultant immobilized PPL exhibited 95 % activity recovery at a maximum loading of 115.3 mg/g. Also, the immobilized PPL surpassed free PPL in pH and temperature tolerances, temperature and storage stabilities, and achieved a 91.6 % yield in benzyl cinnamate biosynthesis. Besides, excellent reusability of the immobilized PPL was demonstrated with an 80.1 % yield after 10 cycles. Overall, these gigaporous microspheres show great potential for the highly efficient immobilization of lipase and enhanced benzyl cinnamate production.
研究领域:
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Huiting Zhu,Xinxin Zheng,Yuanyuan Ou,Ge Wang,Lejing Qu,Qian Li,Xue Zhao,Xinfeng Zhao
Protein functionalized surface has the potential to develop new assays for determining the drug-like properties of potential compounds and discovering specific partners of G protein-coupled receptors (GPCRs). However, a universal method for purifying and immobilizing functional GPCRs has remained elusive. To this end, we developed a general and rapid way to purify and immobilize β 2 -adrenergic receptor (β 2 AR) by silicon-specific peptide. We screened CotB1p as a tag from six silica-binding peptides (minTBP-1, CotB1p, SB 7 , Car 9 , and Si 4–1 ) by examining their affinity to macroporous silica gel. We investigated the adsorption and desorption of CotB1p-tagged β 2 -adrenoceptor (β 2 AR-CotB1p) under diverse conditions to propose a protocol for receptor purification and immobilization. Under optimized conditions, β 2 AR immobilization were achieved by directly immersing cell lysates harboring the receptor with silica gel, and the elution of the receptor without demonstratable contaminants was realized by including l-arginine/L-lysine in the elutes. This allows purification of the receptor from Escherichia coli ( E.coli ) lysates with a purity of 95 %. The immobilized receptor was utilized as a stationary phase to reveal the tag impact on ligand-binding outputs by comparing the CotB1p-strategy with a typical covalent method. The K A s of salbutamol, chlorprenaline, tulobuterol, and terbutaline on β 2 AR-CotB1p column were 1.26 × 10 6 , 6.59 × 10 6 , 7.90 × 10 6 , and 8.97 × 10 5 M −1 respectively, which were two orders of magnitude higher than those on the Halo-β 2 AR column. The whole immobilization was accomplished within 30 min without the requirement of any special treatment, resulting in enhanced accuracy for determining receptor-ligand binding parameters. Taken together, CotB1p-mediated strategy is simple, rapid, and universal for purification or immobilization of unstable biomolecules like GPCRs for analytical and biological applications.
研究领域:
生物化学
·
药学
·
分析化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yixin Li,Zitong Wang,Jiaqi Wu,Jie Zheng,Fu Liu,Juanying Ou,Caihuan Huang,Shiyi Ou
Reactive carbonyl species can modify digestive enzymes upon intake due to their electrophilic nature. This study evaluated the effects of methylglyoxal (MGO), glyoxal, acrolein, and formaldehyde on invertase, an enzyme presents in digestive tract. Unexpectedly, MGO enhanced, rather than inhibited, invertase activity. Moreover, MGO counteracted the inhibitory effects of the other three carbonyls on invertase activity. Kinetic analyses revealed that 150 mmolLexp.-1 MGO resulted in a 2-fold increase in the Km and a 3.3-fold increase in Vmax, indicating that MGO increased the turnover rate of sucrose while reducing the substrate binding affinity of invertase. Additionally, MGO induced dynamic quenching of fluorescence, reduced free amino groups, increased hydrophobicity, the content of Amadori products, fluorescent and nonfluorescent AGEs, and amyloid fibrils of invertase. The specific modifications responsible for the elevated activity of MGO on invertase require further investigation.
研究领域:
生物化学
·
酶学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）