CHINESE CHEMICAL LETTERS
·
2017
·
IF 1.932
Yudong Wu,Weipan Peng,Qian Zhao,Jiafang Piao,Bo Zhang,Xiaoli Wu,Hanjie Wang,Zhihong Shi,Xiaoqun Gong,Jin Chang
At present, many researchers focused on the point-of-care testing (POCT), a method of disease markers detection without large-scale instruments and specialized persons. However, most POCT diagnostic methods were suffered from poor detection sensitivity or inefficiency in quantitative detection. Herein, we developed a newly QD-immune fluorescence test strips (QD-IFTS) based on quantum dots (QDs) as the fluorescence nanocarrier to prepare the immune fluorescence probes in the classical immunochromatography detection system for sensing carcino-embryonic antigen (CEA), a kind of glycoprotein produced by intestinal tissue and a broad spectrum of tumor marker for cancer diagnosis. And we designed a homemade strips fluorescence reader for detection of fluorescence intensity of QDs on the QD-IFTS. Under the optimized reaction conditions, chromatographic time of the newly QD-IFTS was only 25 min, sample volume of the newly QD-IFTS was only 40 μL and the LOD of the newly QD-IFTS was 0.72 ng/mL. In addition, the efficiency and robustness of the newly QD-IFTS were confirmed by successfully application in 300 clinical serum samples, and the results revealed great potential in clinical POCT of other biomarkers.
研究领域:
医学诊断
·
生物化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Micro & Nano Letters
·
2018
·Vol. 13
·期 12
·1719-1723
·
IF 0.841
Wei Chen,Chaoman Ang,Doudou Lou,Yongxin Ji,Ling Huang,Yefei Zhu,Zhirui Guo,Ning Gu,Yu Zhang
The study developed a novel quantitative detection method of homocysteine (Hcy), an independent risk factor for cardiovascular disease, based on an immunofluorescent test strip. The fluorescent nanospheres (F-NSs) were prepared by embedding fluorophores (Cy5) into poly(styrene-acrylate) copolymer nanospheres, then conjugated with streptavidin (SA) to obtain SA/F-NSs as a signal amplification label stored in sample diluents. Biotin–antibody was fixed on the conjugate pad to specifically capture S -adenosyl Hcy (SAH) in the sample, where SAH was transformed from S -adenosyl methionine (SAM) by Hcy S -methyltransferase catalysis using Hcy as a substrate, the formed SAH could be trapped by SA/F-NSs and biotin–antibody conjugates in a competitive way with SAH–bovine serum albumin fixed on the test line, a detection limit of 0.27 μM Hcy was achieved. The fluorescence intensity of F-NSs remained stable during 273 days of storage, the bioactivity of the test strip was stable during 12 months of storage, and the strip possessed good reproducibility (intra-assay variability of 5.8%). Furthermore, other structural analogues SAM and cysteine showed negative results, validating the excellent specificity of the strips.
研究领域:
医学诊断
·
生物化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 23
·8324
·
IF 4.927
Xiuying Liu,Yuanyuan Cheng,Binbin Guan,Fei Xia,Ling Fan,Xue Gao,Xiaofei Sun,Xuepeng Li,Lijie Zhu
A multicolor immunochromatographic assay platform based on quantum dot nanobeads (QBs) for the rapid and simultaneous detection of nitrofuran metabolites in different aquatic products is documented. These metabolites include 3-amino-2-oxazolidinone (AOZ), 1-aminohydantoin (AHD), semicarbazide (SEM), and 3-amino-5-morpholino-methyl-1,3-oxazolidinone (AMOZ). QBs with emission colors of red, yellow, green, and orange were employed and functionalized with the corresponding antibodies to each analyte to develop a multicolor channel. The visual detection limits (cutoff values) of our method for AOZ, AHD, SEM, and AMOZ reached up to 50 ng/mL, which were 2, 20, 20, and 20 times lower than those of traditional colloidal gold test strips, respectively. The test strip is capable of detection within 10 min in real samples while still achieving good stability and specificity. These results demonstrate that the developed multicolor immunochromatographic assay platform is a promising technique for multiplex, highly sensitive, and on-site detection of nitrofuran metabolites.Keywords:nitrofuran metabolites;multiplex immunochromatographic assay;quantum dot nanobeads;fluorescent test strip
研究领域:
分析化学
·
食品科学
·
纳米技术
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Tingting Li,Yukun An,Jiashuang Li,Zhiming Zhang,Lin Liu,Libao An
A bimetallic Ln-MOF nanofibrous membrane (NFM) was successfully fabricated by in situ growth of Eu/Tb-MOF crystals onto electrospun polycaprolactam (PA6) nanofibers . For better combining with PA6 nanofibers , the Eu/Tb-MOFs were made into nanocrystals . Characterization with PXRD , FT-IR, EDS and XPS confirmed that the crystal structure of the Eu/Tb-MOFs maintained well on PA6 NFM. The as-prepared Eu/Tb-MOF@PA6 NFM can be used as a colorimetric fluorescent test strip for instant detection of the volume ratio of 1, 4-dioxane in 1, 4-dioxane-glycol mixture. With the increase of 1, 4-dioxane, the test strip presented color changes from green to red, which can be easily distinguished by the naked eye. In view of this, the conversion rate of the condensation reaction of glycol to 1, 4-dioxane will be preliminarily estimated without the help of equipment. More importantly, the test strip exhibited better convenience and reversibility compared with powdered Ln-MOFs because no separation process was needed for test strip before and after recovering. Benefiting from sufficient amide groups in PA6 for anchoring Eu/Tb-MOF particles, the test strip showed satisfactory stability in cycling experiments. This work will provide a new insight into the development of fluorescent test strip based on electrospun MOF NFM for quantitatively monitoring the reaction process.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Small
·
2018
·Vol. 15
·期 1
·1803913
·
IF 9.598
Jixiang Wang,Jiangdong Dai,Yeqing Xu,Xiaohui Dai,Yunlei Zhang,Weidong Shi,Börje Sellergren,Guoqing Pan
Paper-based assays for detection of physiologically important species are needed in medical theranostics owning to their superiorities in point of care testing, daily monitoring, and even visual readout by using chromogenic materials. In this work, a facile test strip is developed for visual detection of a neurotransmitter dopamine (DA) based on dual-emission fluorescent molecularly imprinted polymer nanoparticles (DE-MIPs). The DE-MIPs, featured with tailor-made DA affinity and good anti-interference, exhibit DA concentration-dependent fluorescent colors, due to the variable ratios of dual-emission fluorescence caused by DA binding and quenching. By facile coating DE-MIPs on a filter paper, the DA test strips are obtained. The resultant test strip, like the simplicity of a pH test paper, shows the potential for directly visual detection of DA levels just by dripping a tiny amount of biofluid sample on it. The test result of real serum samples demonstrates that the DA strip enables to visually and semiquantitatively detect DA within 3 min by using only 10 µL of serum samples and with a low detection limit ((100–150) × 10 −9 m ) by naked eye. This work thus offers a facile and efficient strategy for rapid, visual, and on-site detection of biofluids in clinic.
研究领域:
医学诊断
·
分析化学
·
生物传感器
·
治疗诊断学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Yuqian Liu,Qingsheng Guo,Xiaojun Qu,Qingjiang Sun
The combination of functional nucleic acids and nanomaterials enables the continuous development of hybrid nanosystems that have found wide applications including chemo/biosensing. Herein, we report the supramolecular assembly of a “sesame biscuit”-like superstructural nanosystem based on aptamer, quantum dot (QD), and graphene oxide (GO), and its diverse applications in Pb2+ and pH sensing. The nanosystem was assembled via adsorbing silica-encapsulated green-emitting QD onto the edge of GO by ionic interaction, followed by absorbing aptamer-modified red-emitting QD onto the GO surface via the π-stacking interaction. The nanosystem showed the characteristic of the nonquenched green fluorescence due to silica encapsulation and quenched red fluorescence owing to nanomaterial surface energy transfer. The nanosystem responded to Pb2+/pH in ratiometric fluorescence: the red fluorescence varied upon analyte-driven conformational changes of the aptamer, whereas the green one remained constant. Under optimized conditions, the nanosystem was demonstrated to be capable of quantifying Pb2+ with a detection limit of 11.7 pM, as well as pH with a sensing resolution of 0.1 pH unit. More importantly, the ratiometric nanosystem facilitated visualization of analytes in a distinct “traffic light” manner, which was exemplified by semiquantification of exogenous Pb2+ in living cells. To demonstrate practicality, fluorescent test strips were fabricated by immobilizing the nanosystem on paper. The fluorescent test strips displayed traffic light-type fluorescence color changes, with the capacity for on-site, naked-eye detection of Pb2+ in real samples, as well as point-of-care pH testing in routine urinalysis.
研究领域:
分析化学
·
纳米技术
·
生物传感
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）