Food Chemistry-X
·
2024
·
IF 6.5
Xiuying Liu,Si Kang,Wen Wang,Lijie Zhu,Wei Zhang,Pingping Wang,Zaixi Shu,Yiwei Tang
Histamine is a biogenic amine with various physiological functions. However, excessive consumption of histamine can lead to various symptoms, and pose a threat to human lives. A ratiometric fluorescent test strip for visual detection of histamine was developed based on CB-Ni 2+ @CDs probes. As the concentration of histamine increases, the test strips exhibit a transition in fluorescence signal from yellow-green to blue. The RGB values were extracted from the images, and used for quantitative analysis of histamine. The method had a linear range of 0–1.0 mM, with a detection limit of 0.086 mM. The test strips were employed for the detection of histamine, and the recovery rate was found to be in the range of 88.3% to 104.69%, indicating a high level of accuracy. The uniqueness of the test strips lies in their ability to be produced simply by mixing CB, Ni 2+ on a suitable polyvinyl alcohol/wood cellulose fiber substrate.
研究领域:
生物化学
·
食品安全检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Longjie Wang,Yuchen Zhang,Yibo Chen,Peng Liu,Zhigang Ma,Yanxiong Liu,Linlin Chen,Liyan Zheng,Qiue Cao
Water constitutes the most prevalent impurity in organic solvents, exerting significant influence on chemical reactions and potentially leading to fires and explosions, even in minute quantities. Thus, the development of convenient, rapid, and cost effective fluorescent probes for real-time monitoring of water content in organic solvents is imperative. Although some fluorescent materials have been synthesized for this purpose, most suffer from laborious preparation processes and poor cycling performance, constraining their practical application. This study investigates the impact of hydrogen bonding on the aggregation-induced emission (AIE) properties of quinoline derivatives, leveraging quinoline as the foundational scaffold and its nitrogen atom as the hydrogen bond acceptor. Research findings elucidate that intermolecular hydrogen bonding of quinoline is the primary determinant of their AIE behaviors. By harnessing the phenomenon of water molecules forming intermolecular hydrogen bonds with quinoline nitrogen atoms, we devised a straightforward and rapid method to fabricate a fluorescent test paper for real-time monitoring of water content in organic solvents. Experimental results demonstrate that even minute changes in water content, down to concentrations as low as 0.5 % by volume in organic solvents, can induce fluorescence changes in the test paper, which also exhibits favorable cycling performance. This study not only explores the influence of hydrogen bonds on the AIE properties of quinoline derivatives but also pioneers the development of a cost-effective, rapid, and recyclable test paper for real-time monitoring of water content fluctuations in organic solvents.
研究领域:
化学
·
材料科学
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DOI
PubMed
高影响因子
CHINESE CHEMICAL LETTERS
·
2017
·
IF 1.932
Yudong Wu,Weipan Peng,Qian Zhao,Jiafang Piao,Bo Zhang,Xiaoli Wu,Hanjie Wang,Zhihong Shi,Xiaoqun Gong,Jin Chang
At present, many researchers focused on the point-of-care testing (POCT), a method of disease markers detection without large-scale instruments and specialized persons. However, most POCT diagnostic methods were suffered from poor detection sensitivity or inefficiency in quantitative detection. Herein, we developed a newly QD-immune fluorescence test strips (QD-IFTS) based on quantum dots (QDs) as the fluorescence nanocarrier to prepare the immune fluorescence probes in the classical immunochromatography detection system for sensing carcino-embryonic antigen (CEA), a kind of glycoprotein produced by intestinal tissue and a broad spectrum of tumor marker for cancer diagnosis. And we designed a homemade strips fluorescence reader for detection of fluorescence intensity of QDs on the QD-IFTS. Under the optimized reaction conditions, chromatographic time of the newly QD-IFTS was only 25 min, sample volume of the newly QD-IFTS was only 40 μL and the LOD of the newly QD-IFTS was 0.72 ng/mL. In addition, the efficiency and robustness of the newly QD-IFTS were confirmed by successfully application in 300 clinical serum samples, and the results revealed great potential in clinical POCT of other biomarkers.
研究领域:
医学诊断
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生物化学
·
纳米技术
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产品关联
DOI
高影响因子
Scientific Reports
·
2022
·Vol. 12
·期 1
·1-7
·
IF 4.996
Xiong Jia-Bin,Ban Ding-Ding,Zhou Yong-Juan,Du Hui-Jun,Zhao Ai-Wei,Xie Lan-Ge,Liu Guo-Qun,Chen Si-Ru,Mi Li-Wei
A fluorescent porous organic polymer (FPOP) with strong fluorescence and tunable emission colors, was synthesized through a simple cost-effective method via Scholl coupling reaction. Experiments proved the stability and excellent detection and adsorption ability, and microporous nature of the material. Luminescence of FPOP was quenched when addition of nitroaromatic compounds. The properties along with large-scale and low-cost preparation make these FPOP potential candidates for fluorescence detection of nitroaromatic compounds. Additionally, FPOP shows higher adsorption capacity and rate than other reported adsorbents, and has the possibility of being an effective adsorbent for industrial usage. Moreover, a fluorescent test paper was further developed and is found to be sensitive to 10 –8 M level, complete with a rapid response time and visual detection. This newly developed strategy may open up an avenue for exploring porous polymers, particularly those with a strong fluorescence, for the large-scale fabrication of FPOP for various advanced applications.
研究领域:
材料科学
·
环境科学
·
化学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Xiqing Liu,Tao Wang,Yongqing Wang
In this study, a conversion method and molecular imprinting technology were used to design molecularly imprinted polymers (MIP)-based ratiometric fluorescence test papers. The ZnO quantum dots (ZnO QDs) acted as the background quantum dots and ZIF-8 raw material. Carbon dots (CDs) were used as the identification signals. The imprinting layer achieved a selective function. Therefore, a ZnO@ZIF-8/CDs@MIPs sensor was designed for the detection of Bisphenol A (BPA). The sensor exhibited a fast response time for BPA detection. In addition, the sensor demonstrated that effective detection of BPA can still be achieved in complex environments. The detection limit of this sensor was 0.778 nM with a linear range of 0–60 nM. The corresponding test solutions exhibited clear changes from blue to yellow. The selectivity experiments results demonstrated that ZnO@ZIF-8/CDs@MIPs only exhibit excellent selective recognition effect for BPA. ZnO@ZIF-8/CDs@MIPs-2 was used for the detection of BPA in canned food and compared with the results of HPLC detection of BPA. The two spiked recovery ranges were 96.58–102.04% and 97.43–103.82%, respectively. In addition, the prepared ZnO@ZIF-8/CDs@MIPs-2 test paper visually recognized BPA under ultraviolet light. This study provides guidelines for the design and application of fluorescent test papers for quick detection in practical applications.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
·
2022
·
IF 3.428
Jiali Xu,Yuqi Guo,Tianyu Gong,Kai Cui,Linrui Hou,Changzhou Yuan
It is highly significant to develop water-soluble carbon dots (CDs) for ion detection in the environment via a facile and economical process. Herein, the organic molecules derived boron, nitrogen co-doped CDs (BN-CDs) with water-solubility are solvothermally synthesized towards dual ion detection. The BN-CDs emit bright tangerine fluorescence, and demonstrate a fluorescent quantum yield of 17 %. Besides, the obtained BN-CDs show a good linear relationship with Cu 2+ /Co 2+ in a wide dynamic range of 1–8 μM, along with detection limits of 16 nM (Cu 2+ ) and 24 nM (Co 2+ ). The joint effect of dynamic and static quenching mechanisms is proposed as well for fluorescence quenching by Cu 2+ /Co 2+ . Furthermore, two instrument-free BN-CDs-based solid sensors ( i.e. , test strips and hydrogel beads) are fabricated to sensitively monitor both Cu 2+ and Co 2+ . The paper-based sensors exhibit selective and sensitive response towards Cu 2+ and Co 2+ in a low-cost manner. The fluorescence hydrogel integrates comprehensive advantages of hydrogel and BN-CDs, which makes it not only conduct as efficient fluorescent sensors to detect Cu 2+ and Co 2+ but act as the absorbent towards removal of the ions. The simple yet reliable sensing technique developed here favor efficiently detecting and recognizing dual ions in environmental and/or biological samples.
研究领域:
环境科学
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材料科学
·
分析化学
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DOI
高影响因子
Micro & Nano Letters
·
2018
·Vol. 13
·期 12
·1719-1723
·
IF 0.841
Wei Chen,Chaoman Ang,Doudou Lou,Yongxin Ji,Ling Huang,Yefei Zhu,Zhirui Guo,Ning Gu,Yu Zhang
The study developed a novel quantitative detection method of homocysteine (Hcy), an independent risk factor for cardiovascular disease, based on an immunofluorescent test strip. The fluorescent nanospheres (F-NSs) were prepared by embedding fluorophores (Cy5) into poly(styrene-acrylate) copolymer nanospheres, then conjugated with streptavidin (SA) to obtain SA/F-NSs as a signal amplification label stored in sample diluents. Biotin–antibody was fixed on the conjugate pad to specifically capture S -adenosyl Hcy (SAH) in the sample, where SAH was transformed from S -adenosyl methionine (SAM) by Hcy S -methyltransferase catalysis using Hcy as a substrate, the formed SAH could be trapped by SA/F-NSs and biotin–antibody conjugates in a competitive way with SAH–bovine serum albumin fixed on the test line, a detection limit of 0.27 μM Hcy was achieved. The fluorescence intensity of F-NSs remained stable during 273 days of storage, the bioactivity of the test strip was stable during 12 months of storage, and the strip possessed good reproducibility (intra-assay variability of 5.8%). Furthermore, other structural analogues SAM and cysteine showed negative results, validating the excellent specificity of the strips.
研究领域:
医学诊断
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生物化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry C
·
2016
·Vol. 4
·期 42
·10105-10110
·
IF 5.066
Shuailing Huang,Yinglong Wu,Fang Zeng,Lihe Sun,Shuizhu Wu
Exposure to odorless, colorless, tasteless and yet lethal nerve gases could lead to paralysis of the central nervous system, organ failure or even rapid death. Hence it is of great importance to develop a convenient, portable and rapid detection method for these gaseous nerve agents. Herein, based on the aggregation-induced emission (AIE) characteristics, a ratiometric fluorescent test-strip sensor (DPA-TPE-Py) for rapid, sensitive and selective detection of the gaseous nerve agent simulant diethyl chlorophosphate (DCP) has been developed. It is easy to prepare handy and portable solid test strips via direct deposition of AIE probes onto the filter paper. Moreover, when the test strips are exposed to DCP vapor, their fluorescence changes from yellow to orange red. The detection limit for DCP is as low as 1.82 ppb, lower than the reported Immediately Dangerous to Life or Health concentration (IDLH concentration). Such a strategy could provide helpful insights into designing convenient cost-effective ratiometric fluorescent sensors in the solid state as well as affording a portative method for detection of gaseous nerve agents.
研究领域:
化学传感
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环境监测
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 23
·8324
·
IF 4.927
Xiuying Liu,Yuanyuan Cheng,Binbin Guan,Fei Xia,Ling Fan,Xue Gao,Xiaofei Sun,Xuepeng Li,Lijie Zhu
A multicolor immunochromatographic assay platform based on quantum dot nanobeads (QBs) for the rapid and simultaneous detection of nitrofuran metabolites in different aquatic products is documented. These metabolites include 3-amino-2-oxazolidinone (AOZ), 1-aminohydantoin (AHD), semicarbazide (SEM), and 3-amino-5-morpholino-methyl-1,3-oxazolidinone (AMOZ). QBs with emission colors of red, yellow, green, and orange were employed and functionalized with the corresponding antibodies to each analyte to develop a multicolor channel. The visual detection limits (cutoff values) of our method for AOZ, AHD, SEM, and AMOZ reached up to 50 ng/mL, which were 2, 20, 20, and 20 times lower than those of traditional colloidal gold test strips, respectively. The test strip is capable of detection within 10 min in real samples while still achieving good stability and specificity. These results demonstrate that the developed multicolor immunochromatographic assay platform is a promising technique for multiplex, highly sensitive, and on-site detection of nitrofuran metabolites.Keywords:nitrofuran metabolites;multiplex immunochromatographic assay;quantum dot nanobeads;fluorescent test strip
研究领域:
分析化学
·
食品科学
·
纳米技术
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2024
·
IF 4.9
Tingting Li,Yukun An,Jiashuang Li,Zhiming Zhang,Lin Liu,Libao An
A bimetallic Ln-MOF nanofibrous membrane (NFM) was successfully fabricated by in situ growth of Eu/Tb-MOF crystals onto electrospun polycaprolactam (PA6) nanofibers . For better combining with PA6 nanofibers , the Eu/Tb-MOFs were made into nanocrystals . Characterization with PXRD , FT-IR, EDS and XPS confirmed that the crystal structure of the Eu/Tb-MOFs maintained well on PA6 NFM. The as-prepared Eu/Tb-MOF@PA6 NFM can be used as a colorimetric fluorescent test strip for instant detection of the volume ratio of 1, 4-dioxane in 1, 4-dioxane-glycol mixture. With the increase of 1, 4-dioxane, the test strip presented color changes from green to red, which can be easily distinguished by the naked eye. In view of this, the conversion rate of the condensation reaction of glycol to 1, 4-dioxane will be preliminarily estimated without the help of equipment. More importantly, the test strip exhibited better convenience and reversibility compared with powdered Ln-MOFs because no separation process was needed for test strip before and after recovering. Benefiting from sufficient amide groups in PA6 for anchoring Eu/Tb-MOF particles, the test strip showed satisfactory stability in cycling experiments. This work will provide a new insight into the development of fluorescent test strip based on electrospun MOF NFM for quantitatively monitoring the reaction process.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Small
·
2018
·Vol. 15
·期 1
·1803913
·
IF 9.598
Jixiang Wang,Jiangdong Dai,Yeqing Xu,Xiaohui Dai,Yunlei Zhang,Weidong Shi,Börje Sellergren,Guoqing Pan
Paper-based assays for detection of physiologically important species are needed in medical theranostics owning to their superiorities in point of care testing, daily monitoring, and even visual readout by using chromogenic materials. In this work, a facile test strip is developed for visual detection of a neurotransmitter dopamine (DA) based on dual-emission fluorescent molecularly imprinted polymer nanoparticles (DE-MIPs). The DE-MIPs, featured with tailor-made DA affinity and good anti-interference, exhibit DA concentration-dependent fluorescent colors, due to the variable ratios of dual-emission fluorescence caused by DA binding and quenching. By facile coating DE-MIPs on a filter paper, the DA test strips are obtained. The resultant test strip, like the simplicity of a pH test paper, shows the potential for directly visual detection of DA levels just by dripping a tiny amount of biofluid sample on it. The test result of real serum samples demonstrates that the DA strip enables to visually and semiquantitatively detect DA within 3 min by using only 10 µL of serum samples and with a low detection limit ((100–150) × 10 −9 m ) by naked eye. This work thus offers a facile and efficient strategy for rapid, visual, and on-site detection of biofluids in clinic.
研究领域:
医学诊断
·
分析化学
·
生物传感器
·
治疗诊断学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Yuqian Liu,Qingsheng Guo,Xiaojun Qu,Qingjiang Sun
The combination of functional nucleic acids and nanomaterials enables the continuous development of hybrid nanosystems that have found wide applications including chemo/biosensing. Herein, we report the supramolecular assembly of a “sesame biscuit”-like superstructural nanosystem based on aptamer, quantum dot (QD), and graphene oxide (GO), and its diverse applications in Pb2+ and pH sensing. The nanosystem was assembled via adsorbing silica-encapsulated green-emitting QD onto the edge of GO by ionic interaction, followed by absorbing aptamer-modified red-emitting QD onto the GO surface via the π-stacking interaction. The nanosystem showed the characteristic of the nonquenched green fluorescence due to silica encapsulation and quenched red fluorescence owing to nanomaterial surface energy transfer. The nanosystem responded to Pb2+/pH in ratiometric fluorescence: the red fluorescence varied upon analyte-driven conformational changes of the aptamer, whereas the green one remained constant. Under optimized conditions, the nanosystem was demonstrated to be capable of quantifying Pb2+ with a detection limit of 11.7 pM, as well as pH with a sensing resolution of 0.1 pH unit. More importantly, the ratiometric nanosystem facilitated visualization of analytes in a distinct “traffic light” manner, which was exemplified by semiquantification of exogenous Pb2+ in living cells. To demonstrate practicality, fluorescent test strips were fabricated by immobilizing the nanosystem on paper. The fluorescent test strips displayed traffic light-type fluorescence color changes, with the capacity for on-site, naked-eye detection of Pb2+ in real samples, as well as point-of-care pH testing in routine urinalysis.
研究领域:
分析化学
·
纳米技术
·
生物传感
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）