FOOD CHEMISTRY
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2022
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IF 9.231
Zhenhua Wen,Xuelian Hu,Rongfang Yan,Wenxiu Wang,He Meng,Yang Song,Shuo Wang,Xianghong Wang,Yiwei Tang
Olaquindox residues in food from its illegal use has received great attention. Here, an immunoassay strategy integrating an upconversion nanoparticle (UCNP)-based immunochromatographic strip with a fluorescence reader was proposed for the highly selective and sensitive detection of olaquindox. Polyacrylic acid-functionalized UCNPs were synthesized using a simple ligand exchange process and combined with an olaquindox polyclonal antibody to form a fluorescent probe. This approach achieved a sensitive response and specific recognition of olaquindox. A convenient upconversion fluorescence reader was introduced to implement accurate and sensitive quantitative analysis of olaquindox based on the fluorescence intensity of control and test lines on a strip. Under optimal conditions, the method demonstrated a favorable linear range (0–50 ng/mL) and sensitive detection (1.42 ng/mL, S/N = 3). This method was applied successfully to determine olaquindox in fish muscle and water samples, and results were consistent with an HPLC approach, and considered a promising strategy for monitoring olaquindox residuals.
研究领域:
分析化学
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环境科学
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食品安全
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DOI
PubMed
高影响因子
ANALYST
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2021
·Vol. 146
·期 24
·7702-7709
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IF 4.616
Lei Huang,Shulin Tian,Wenhao Zhao,Ke Liu,Xing Ma,Jinhong Guo,Ming Yin
Acute myocardial infarction (AMI) has become a worldwide health problem because of its rapid onset and high mortality. Cardiac troponin I (cTnI) is the gold standard for diagnosis of AMI, and its rapid and accurate detection is critical for early diagnosis and management of AMI. Using a lateral flow immunoassay with upconverting nanoparticles as fluorescent probes, we developed an up-conversion fluorescence reader capable of rapidly quantifying the cTnI concentration in serum based upon the fluorescence intensity of the test and control lines on the test strip. Reliable detection of cTnI in the range 0.1–50 ng mL−1 could be achieved in 15 min, with a lower detection limit of 0.1 ng mL−1. The reader was also adapted for use on a 5th generation (5G) mobile network enabled intelligent chest pain center. Through Bluetooth wireless communication, the results achieved using the reader on an ambulance heading to a central hospital could be transmitted to a 5G smartphone and uploaded for real-time edge computing and cloud storage. An application in the 5G smartphone allows users to upload their medical information to establish dedicated electronic health records and doctors to monitor patients’ health status and provide remote medical services. Combined with mobile internet and big data, the 5G-enabled intelligent chest pain center with up-conversion lateral flow immunoassay may predict the onset of AMI and save valuable time for patients suffering an AMI.
研究领域:
远程医疗
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医学信息学
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心脏病学
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生物医学工程
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纳米技术
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移动健康
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2018
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IF 5.123
Tao Peng,Jianyi Wang,Sijun Zhao,Yuyang Zeng,Pimiao Zheng,Demei Liang,Ghulam Mujtaba Mari,Haiyang Jiang
A multiplex lateral flow immunoassay sensor based on highly luminescent green-emitting Au nanoclusters (AuNCs-MLFIA sensor) was successfully established for the simultaneous and quantitative determination of clenbuterol (Clen) and ractopamine (RAC) in swine urine. The antigens of Clen and RAC were dispersed on a nitrocellulose membrane as two test lines, and the Au nanoclusters were synthesized from 6-aza-2-thiothymine and l -arginine to obtain highly green luminescence and ultra-small nanoparticles (Arg/ATT/AuNCs). Free carboxyl groups on Arg/ATT/AuNCs enabled conjugation with biomolecules to afford an indicator for the biosensor . The AuNCs-MLFIA sensor is based on the indirect competition assay and could successfully detect samples within 18 min without sample pretreatment , qualitative results can be obtained by visual inspection under a UV lamp. The limits of detection of Clen and RAC by the naked eye were both 0.25 μg L −1 . In addition, the AuNCs-MLFIA sensor allowed quantitative detection combined with a portable fluorescence reader. The half-maximal inhibitory concentrations of Clen and RAC were 0.06 and 0.32 μg L −1 , respectively, with detection limits of 0.003 and 0.023 μg L −1 . Thirty blind-spiked swine urine samples were analyzed by the AuNCs-MLFIA sensor and liquid chromatography–tandem mass spectrometry, and the results of the two methods showed a significant correlation. The newly developed AuNCs-MLFIA sensor overcomes several limitations of conventional LFIA sensors, including their low sensitivity, limitation to quantify analytes , and single-analyte detection.
研究领域:
分析化学
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纳米技术
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生物传感器
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食品安全
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产品关联
DOI
PubMed
高影响因子
LAB ON A CHIP
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2023
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IF 6.1
Yulin Ren,Jingcheng Ji,Haoqing Zhang,Lei Cao,Jie Hu,Feng Xu,Zedong Li
Droplet digital polymerase chain reaction (ddPCR) technology has found widespread applications in the ultrasensitive analysis of nucleic acids, where integrated ddPCR platforms with the capability of sample dispersion, followed by in situ amplification and data analysis, are highly expected. However, current integrated ddPCR platforms are usually limited by either difficultly mass-produced materials or lack of integrated control instruments, restricting their practical application. This paper proposes an integrated three-in-one ddPCR platform with high user-friendliness and practicability, which is composed of an easy-to-use chip and a matching control instrument. The chip was made of thermally resistant and easily mass-produced polycarbonate (PC) material, and the benchtop control instrument was designed to perform droplet generation, in situ amplification, and fluorescence reading. The droplet generation and in situ heating on the chip were well characterized. Finally, the performance of the platform was validated through the analysis of the EGFR L858R mutation in lung cancer. The proposed three-in-one ddPCR platform shows great practicability in ultrasensitive nucleic acid testing. By virtue of its sensitivity, practicability, and cost-effectiveness, the ddPCR can serve as a universal detection platform for monitoring nucleic acid in the fields of tumor diagnosis, pathogen detection, and prenatal diagnosis.
研究领域:
生物技术
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分子生物学
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医学诊断
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基因工程
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2022
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IF 6.911
Yuan Su,Xuetong Li,Longjiao Zhu,Huashuo Chu,Yangzi Zhang,Jingjing Tian,Shuxia Lyu,Wentao Xu
To develop an on-site, thermostatic and rapid sensor for the detection of Vibrio parahaemolyticus (V. parahaemolyticus) , A single cross-priming fluorescence (SCPF) sensor was designed using a 3D nano-nucleic acid hybrid material that termed mesoporous silica nanoparticle/nucleic acid-doped nanoflower (MSN/NA-doped nanoflower). In addition, a portable polymerase chain reaction (PCR) tube fluorescence reader was built. Further analysis of the MSN/NA-doped nanoflower morphology and the enhancement mechanism indicated that the MSNs aggregated into larger nanoclusters by adsorbing single cross-priming amplification (sCPA) components, forming MSNs/NAs-doped nanoflowers, and increasing the local concentrations to enhance sCPA efficiency. Cyclic amplification relied mainly on the self-folding hairpin-like structure of the amplified product that continuously formed, opened, re-formed, and opened again. The target DNA was detected with a detection limit of 2.4 copies/μL.
研究领域:
生物技术
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分子生物学
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分析化学
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微生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）