Chemical Biology & Drug Design
·
2019
·Vol. 94
·期 2
·1494-1503
·
IF 2.256
Xi Li,Qingzhu Liu,Siqin Ye,Shijie Wang,Ke Li,Gaochao Lv,Ying Peng,Ling Qiu,Jianguo Lin
Legumain, a lysosomal cysteine protease, is critical for pathological progression and has been found to play an important role in the occurrence and development of several cancers. However, its biological functions remain few recognized. To further understand the role of legumain activity in tumor progression, a legumain protease-responsive fluorescent probe was developed in the present study. The probe 1 was synthesized by conjugating an aminoluciferin fluorophore with an alanine–alanine–asparagine (AAN) peptide sequence. The successful synthesis of probe 1 was validated by NMR and MS spectra as well as HPLC analysis. The probe 1 was non-toxic and exhibited great stability in the physiological solutions. More importantly, compared with the aminoluciferin fluorophore, the peptide conjugation may dramatically increase the targeting specificity. Probe 1 was able to effectively detect the legumain activity in living HCT116 cells through fluorescence imaging. All these results implied that probe 1 could act as a promising fluorescent probe specialized for the monitoring of legumain activity in living cells.
研究领域:
生物化学
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分子生物学
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癌症研究
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细胞生物学
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高影响因子
Small
·
2017
·Vol. 13
·期 26
·1700075
·
IF 8.643
Jiangling He,Youling He,Yonghao Chen,Bingfu Lei,Jianle Zhuang,Yong Xiao,Yeru Liang,Mingtao Zheng,Haoran Zhang,Yingliang Liu
Stable solid-state red fluorescence from organosilane-functionalized carbon dots (CDs) with sizes around 3 nm is reported for the first time. Meanwhile, a novel method is also first reported for the efficient construction of dual-fluorescence morphologies. The quantum yield of these solid-state CDs and their aqueous solution is 9.60 and 50.7%, respectively. The fluorescence lifetime is 4.82 ns for solid-state CDs, and 15.57 ns for their aqueous solution. These CDs are detailedly studied how they can exhibit obvious photoluminescence overcoming the self-quenching in solid state. Luminescent materials are constructed with dual fluorescence based on as-prepared single emissive CDs (red emission) and nonfluorescence media (starch, Al 2 O 3 , and RnOCH 3 COONa), with the characteristic peaks located at nearly 440 and 600 nm. Tunable photoluminescence can be successfully achieved by tuning the mass ratio of CDs to solid matrix (such as starch). These constructed dual-fluorescence CDs/starch composites can also be applied in white light-emitting diodes with UV chips (395 nm), and oxygen sensing.
研究领域:
材料科学
·
光学
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PubMed
高影响因子
TETRAHEDRON
·
2020
·
IF 2.233
Jian Chen,Kai Chen,Baichuan Han,Yunting Xue,Wanjuan Chen,Zhenzhong Gao,Xianfeng Hou
A single-fluorophore-based ratiometric fluorescent probe based on anthracene carboximide was developed for detection of formaldehyde. In the presence of formaldehyde, the fluorescence emission would change from red to yellow-green, and the probe showed excellent sensitivity, selectivity and anti-interference. Furthermore, the response mechanism was illustrated to be that change in fluorescent signal is resulted from variation of substituent group on the 6th position of anthracene ring. A test paper was developed by loading probe onto filter paper strip. With this test paper, the detection of gaseous formaldehyde could be carried out conveniently, visually and quickly. 2009 Elsevier Ltd. All rights reserved.
研究领域:
分析化学
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环境科学
·
生物医学
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DOI
高影响因子
ARABIAN JOURNAL FOR SCIENCE AND ENGINEERING
·
2024
·
IF 2.6
Li Fen,Yu Xuemin,Xu Jicai,Di Mohan,Song Hongzan,Run Mingtao
In order to prepare white fluorescent emitting materials, poly(ethylene glycol succinate- co -2,5-furandicarboxylic acid ethylene glycol ester) (PESF) blue fluorescent copolyester was prepared by melt polycondensation, and an orange fluorescent copolyester was prepared by introducing 1,6,7,12-tetrachloro-3,4,9,10-perylene tetracarboxylic acid dianhydride, as one of the fluorescent units, into the molecular chain of poly(butylene glycol isophthalate- co -butylene glycol terephthalate)(PBIT). 4,4-dicyclohexylmethane diisocyanate (HMDI) was used as a chain extender for linking PESF and PBIT to obtain the white fluorescent copolyester. The results showed that introducing 2,5-furandicarboxylic acid (FDCA) into the molecular chain of aliphatic polyesters can significantly enhance the blue fluorescence intensity of the PESF copolyesters, and white-light emission with CIE coordinates of (0.31, 0.35) and (0.30, 0.34) was achieved for the copolyester by varying the content of in the PESF and the copolymerization ratio of PESF and PBIT. In addition, by adjusting the magnitude of the excitation wavelength, it is possible to achieve the fine-tuning of the light color of the white copolyester in a certain region, e.g., the CIE coordinates at 350 nm excitation are (0.32, 0.35). The white copolyester displayed high thermal decomposition temperature about 350 o C, remarkably increased elongation at break above 300%, relatively high tensile strength up to 33.2 MPa; therefore, these performance advantages make enhance their potential for applications.
研究领域:
材料科学
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化学工程
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高影响因子
JOURNAL OF BIOMEDICAL OPTICS
·
2007
·
IF 2.87
Doriel Verdes,Thomas Ruckstuhl,Stefan Seeger
We report a new two-channel fluorescence microscopy technique for surface-generated fluorescence. The realized fluorescence microscope allows high resolution imaging of aqueous samples. The core element of the instrument is a parabolic mirror objective that is used to collect the fluorescence at large surface angles above the critical angle of the water/glass interface. An aspheric lens, incorporated into the solid parabolic element, is used for diffraction-limited laser focusing and for collecting fluorescence at low angles with respect to the optical axis. By separated collection of the fluorescence emitted into supercritical and subcritical angles, two detection volumes strongly differing in their axial resolution are generated at the surface of a glass cover slip. The collection of supercritical angle fluorescence (SAF) results in a strict surface confinement of the detection volume, whereas collecting below the critical angle allows gathering the fluorescence emitted several microns deep inside the sample. Consequently, the signals from surface-bound and unbound diffusing fluorescent molecules can be obtained simultaneously.
研究领域:
生物物理学
·
光学成像
·
分子生物学
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DOI
高影响因子
TALANTA
·
2022
·
IF 6.556
Jinli Fu,Shu Zhou,Sisi Tang,Xiaodan Wu,Pengfei Zhao,Kangling Tang,Yu Chen,Zhaoxia Yang,Zhaohui Zhang
The rapid and simple synthesis of highly efficient multi-emission luminescent metal-organic frameworks (LMOFs) with down/up-conversion fluorescence properties becomes critical in potential optical sensing. Here, we present a luminescent dual-ligand co-assisted strategy to one-pot prepare LMOFs for multiplex fluorescence sensing using carbon dots (CDs) and 2-methylimidazole (2-MIM) as dual-ligand to induce Zn 2+ on zeolitic imidazolate framework-8 surface (CDs/MIM-Zn). The CDs/MIM-Zn exhibited efficient fluorescence-enhanced dual channels triple-emission with Stokes and anti-Stokes type excitation profiles. With the assistance of Stokes and anti-Stokes type optical property of CDs and the intrinsic porosity of MOFs structure, a rapid and sensitive dual-channel detection excited at 370 nm and 790 nm was performed to fluorescence turn off-on detect metal ions and reduced glutathione in real blood samples with the satisfactory recoveries of 98.3–106.4%. The dual channels triple-emission strategy ensured multiplex detection of more analytes in parallel.
研究领域:
化学
·
材料科学
·
分析化学
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PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 21
·7379
·
IF 4.927
Yutong Li,Xinhui Gu,Jiayin Zhao,Fengna Xi
The construction of novel fluorescent nanozymes is highly desirable for providing new strategies for nanozyme-based sensing systems. Herein, a novel ratiometric fluorescence sensing platform was constructed based on carbon dots (CDs) as both luminophores and nanozymes, which could realize the sensitive detection of hydrogen peroxide (H2O2). CDs with peroxidase-mimicking activity were prepared with a one-step hydrothermal method usingL-histidine as an inexpensive precursor. CDs had bright blue fluorescence. Due to the pseudo-peroxidase activity, CDs catalyzed the oxidation of o-phenylenediamine (OPD) with H2O2to generate 2,3-diaminophenolazine (DAP). The fluorescence resonance energy transfer (FRET) between CDs and DAP resulted in a decrease in the fluorescence of CDs and an increase in the fluorescence of DAP, leading to a ratiometric fluorescence system. The free radical trapping experiment was used to investigate the reactive oxygen radicals (ROS) in the catalytic process of CD nanozymes. The enzymatic parameters of CD nanozymes, including the Michaelis constant (Km) and the maximum initial reaction velocities (Vmax), were investigated. A good affinity for both OPD and H2O2substrates was proven. Based on the FRET between CDs and OPD, a ratiometric fluorescence analysis of H2O2was achieved and results ranged from 1 to 20 μM and 20 to 200 μM with a low limit of detection (LOD, 0.42 μM). The detection of H2O2in milk was also achieved.Keywords:fluorescent nanozymes;carbon dots;ratiometric fluorescence;fluorescence resonance energy transfer;hydrogen peroxide
研究领域:
生物化学
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分析化学
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RESEARCH ON CHEMICAL INTERMEDIATES
·
2017
·Vol. 44
·期 1
·491-502
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IF 1.369
Li Shaoxin,Zhao Bing,Kan Wei,Wang Liyan,Song Bo,Chen Shuwen
A novel pH fluorescence probe (PIF), derived from phenanthro[9,10- d ]imidazole bound to the fluorescein, is designed and synthesized based on the intramolecular hydrogen bond and then its spectra properties are investigated. Probe PIF exhibits negligible fluorescence intensity at acidic pH condition due to ESIPT quenching caused by the formation of the intramolecular hydrogen bonds, and a significant fluorescence enhancement in neutral and weak alkaline environments because of the strengthened ICT process arose from the fracture of the intramolecular hydrogen bonds. The studies of UV–Vis and fluorescence spectra mean that probe PIF is able to respond linearly and reversibly over pH from 6.5 to 9.0 with p K
a of 7.0. Moreover, the pH responses of probe PIF are stable and not disturbed by the common cations and anions. The possible response mechanism has been proposed according to the analysis of 1 H NMR and DFT calculations.
研究领域:
分析化学
·
生物化学
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高影响因子
Nanoscale
·
2018
·Vol. 10
·期 12
·5532-5538
·
IF 7.233
Bi-Yun Fang,Cheng Li,Jie An,Sun-Duo Zhao,Zhi-Yong Zhuang,Yuan-Di Zhao,Yue-Xuan Zhang
In this paper, DNA containing six cytosines as the formation site for silver nanoclusters (Ag NCs) was adopted as a template for preparing fluorescent DNA-Ag NCs. For the first time, it was found that the fluorescence of DNA-Ag NCs could be quenched after hybridization with their complementary sequence. On the basis of this new phenomenon, we designed a sequence C1 that was completely complementary to human immunodeficiency virus (HIV) DNA, and probe DNA which was partially complementary to C1 for the synthesis of DNA-Ag NCs. The fluorescence of DNA-Ag NCs was quenched after hybridization with C1 and the DNA-Ag NCs/C1 composite was formed, while C1 could be dissociated away from the DNA-Ag NCs by HIV DNA through a strand exchange reaction due to the stronger affinity between HIV DNA and C1, which could switch on the quenched Ag NCs, thus a new “off–on” fluorescence method for HIV DNA detection was developed. In the experiment, the Ag NCs formation site of DNA, the number of base pairs, and the pH and salt concentration of binding buffer were optimized. Under the optimum conditions, the limit of detection for HIV DNA was obtained to be 3.18 nM (3σ/N, n = 7) with the linear range of 15–150 nM for the 150 nM DNA-Ag NCs/C1 probe. Besides, the probe showed excellent specificity to HIV DNA, and even distinguished one nucleotide mismatched HIV DNA.
研究领域:
生物技术
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纳米技术
·
分子生物学
·
病毒学
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2016
·
IF 2.653
Huabing Liu,Chaoyong Xu,Yanli Bai,Lin Liu,Dongmei Liao,Jiangong Liang,Lingzhi Liu,Heyou Han
Carbon dots (CDs) have been widely used for the preparation of multifunctional probes by conjugation with organic fluorescent dyes . However, the effect of organic fluorescent dyes on CDs still remains poorly understood. Herein, the effect of fluorescein isothiocyanate (FITC) on CDs was explored by spectroscopic techniques at pH 5.1, 7.0 and 9.0. The fluorescent intensity of CDs was found to be quenched gradually after mixing directly with different concentrations of FITC, but the fluorescent lifetime of CDs remained unchanged. According to the results of UV–vis absorption spectra and fluorescent lifetime measurements, a pH-dependent inner filter effect (IFE) between CDs and FITC was proposed. However, the fluorescent lifetime of CDs deceased after their conjugation with FITC, implying the fluorescence resonance energy transfer (FRET) between CDs and FITC. This study has revealed two different effects of FITC on CDs with varying pH values and provided useful theoretical guidelines for further research on the interaction between other nanoparticles and fluorophores.
研究领域:
生物化学
·
纳米生物学
·
光学
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PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Fangfang Li,Xuan Hu,Fengyi Wang,Baozhan Zheng,Juan Du,Dan Xiao
A simple, rapid, and ultrasensitive fluorescence strategy for adenosine triphosphate (ATP) detection was developed by using a FAM (carboxyfluorescein) labeled DNA (FAM-DNA). In this strategy, highly fluorescent FAM-DNA was used as a probe, and nanoceria (CeO 2 NPs) acted as an efficient quencher. FAM-DNA attached to the surface of nanoceria through the coordination between the phosphate group of DNA and NP surface, which induced complete quenching in the FAM-DNA fluorescence due to a photo induced electron transfer (PET) process. It was found that ATP can readily displace adsorbed DNA from nanoceria surface because of the stronger coordination ability of ATP with nanoceria, and the nanoceria-based competitive binding resulted in over 7-fold fluorescence enhancement. Over a wide range from 0.1 nM to 1.5 μM, a good linear relationship between the fluorescence intensity and the concentration of ATP was obtained and the detection limit was estimated to be as low as 54 pM. This method was successfully used to analyze ATP in a single drop of blood and human urine.
研究领域:
生物化学
·
分析化学
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PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Qian Sun,Lu Ren,Jing Liu,Zhaoyun Yang,Dawei Zhang,Shuangbao Li
A Schiff base fluorescent probe, HTT, based on a sulfonylhydrazone structure was designed and synthesized for the sensitive and selective detection of Cu2+. The probe HTT exhibits good anti-interference performance toward Cu2+ in the presence of a variety of metal ions. After the addition of Cu2+, it can quickly respond within 40 seconds, and the fluorescence detection limit is 1.10 nM. The coordination ratio of probe HTT and Cu2+ is 2 : 1, and the coordination reaction between CN, SO and Cu2+ limits the formation of hydrogen bonds between the hydroxyl group and CN, disrupting the spatial coplanar effect of the probe molecule and thereby inducing fluorescence quenching. The probes can be recovered and reused using EDTA for the detection of Cu2+. The probe was also applied to the successful monitoring of Cu2+ in living cells and real water samples.
研究领域:
分析化学
·
生物化学
·
细胞生物学
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PubMed
高影响因子
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