INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2024
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IF 5.3
Yuqi Li,Jifeng Shi,Xinyu Zhang,Meng Ji,Yifei Ni,Ruiying Han,Zixuan Li,Yerong Xiong,Jiasheng Tu,Dongsheng He,Chunmeng Sun
Surface tension is a crucial functional indicator for various classes of pharmaceutical excipients, as highlighted in both the Pharmacopoeia of the People’s Republic of China (ChP) <9601 Guidelines for Functional Indicators of Pharmaceutical Excipients> and the United States Pharmacopoeia (USP) <1059 Excipient Performance>. However, there are few systematic studies on surface tension measurement of pharmaceutical excipients, resulting in a lack of stable parameter support in practical applications. In this study, we aim to fill this gap by exploring three different methods for measuring surface tension. These methods were carefully developed taking into account the actual measurement process and statistical theory, thus ensuring their applicability and reliability. Through comparative analyses, we have identified the most suitable measurement methods for different classes of pharmaceutical excipients. In addition, this paper describes the surface adsorption behavior of various excipients. Therefore, this study provides valuable guidance for the determination of surface tension and the study of surface adsorption behavior, which lays the foundation for further comprehensive research in the field of surface tension of pharmaceutical excipients and the improvement of general pharmacopoeia specification.
研究领域:
药学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROTECTION
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2019
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IF 1.559
Xinxin Liu,Liwu Wang,Hao Li,Juxin Yin,Shaowu Lv,Ketong Zhu
ABSTRACT The purpose of this study was to investigate the status of organochlorine pesticide residues in 186 ginseng samples collected in Jilin, People's Republic of China. Based on the Chinese Pharmacopoeia 2015 method for detection of organochlorine pesticide residues in ginseng, 22 organochlorine pesticide residues were identified. Chlordane, aldrin, epichlorohydrin, and dieldrin and their isomers were not detected in ginseng from this region. Heptachlor was detected in only one ginseng sample, and the concentration did not exceed the maximum residual limit (MRL) prescribed in the Pharmacopoeia (0.05 mg/kg). Benzene hexachloride was detected in two samples, one of which was above the MRL. Hexachlorobenzene and pentachloronitrobenzene (quintozene) were found in 11.8 and 52.1% of the samples, respectively, and the residues in these samples exceeded the MRL by 4.3 and 8.6%, respectively. HIGHLIGHTS Eight organochlorine pesticides and their isomers were detected in ginseng from Jilin, China. The most common organochlorine pesticide, quintozene, was detected in 52.15% of the samples. Only 9% of ginseng samples contained organochlorine pesticides above the MRL.
研究领域:
药学
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环境科学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
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IF 9.221
Yuchen Zhu,Jian Xu,Haiyan Zhang,Aodong Zhang,Boxin Chen,Bin Zhao,Lei Li,Xuhong Qian,Ya Cheng,Fei Feng
A novel micro gas chromatography column (μGCC) with straight-sided elliptic microchannel cross sections was prepared by ultrafast laser-assisted chemical etching inside a glass substrate . The straight-sided elliptic cross sections resulted in a uniform distribution of the flow velocity field . This uniform distribution and the use of a microchannel with a consistent composition led to a uniform stationary phase coating. Upon the separation of a test mixture of C7–C11 alkanes, highly symmetrical chromatographic peaks were obtained. The tailing factors of the chromatographic peaks ranged from 0.95 to 1.05, which satisfies the relevant provisions of Pharmacopoeia of the People's Republic of China (ChP). The test results show that the improvement of the tailing factors is mainly due to the optimization of the cross-section structure rather than the optimization of the test conditions. Notably, due to the simple preparation processes and the low cost of the glass substrate, the total manufacturing cost of this novel μGCC is low.
研究领域:
分析化学
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材料科学
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产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2022
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IF 3.571
Dongyuan Lu,Zhipeng Wang,Shouhong Gao,Yuehao Han,Hongsen Wang,Hanglin Li,Hongxia Yan,Bosu Meng,Yuhui Mu,Yi Jiang,Xia Tao,Wansheng Chen
Depression is a mental health disorder characterized by chronic negative mood, and depression has become a major threat to human health and quality of life. Anyupeibo capsule , a fifth-class new Chinese medicine, was prepared with extracts of Piper laetispicum C.DC. (Piperaceae), and the alkaloid K6 (5′-methoxy-3′,4′-methyl-enedioxycinnamic-acidisob-utylamide-isobutylamide) was found to be the main active component. Using high-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (LC-MS/MS), we developed a method to quantify the concentration of K6 in serum samples from patients with depression. Pretreatment of samples was completed based on solid-phase extraction, and the mobile phase for subsequent LC analysis consisted of aqueous ammonium acetate (0.1 mmol/L, phase A) and acetonitrile (phase B) with isocratic elution at 60% B. Chromatographic separation of K6 was achieved within 3 min with an Agilent ZORBAX SB-C 18 column (2.1 × 150 mm, 3.5 µm) at a flow rate of 0.3 mL/min. A linear regression equation for K6 yielded correlation coefficients of r 2 > 0.99 within a linear range 0.0503–100.5000 ng/mL. Extraction recovery ranged from 85.33% to 101.18%, and the matrix effect ranged from 87.15% to 100.28%. The inter-day and intra-day precision values—expressed as relative standard deviation—were less than 15%, and the corresponding accuracy values were within ±15%. All validation results for stability, specificity, and carry-over met the requirements of Pharmacopoeia. The LC-MS/MS method was applied to determine the K6 concentration in serum samples from patients with depression in a phase III clinical trial of Anyupeibo capsule.
研究领域:
药理学
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分析化学
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临床试验
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心理健康
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产品关联
DOI
PubMed
高影响因子
MOLECULES
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2024
·Vol. 29
·期 11
·2560
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IF 4.2
Qundong Liu,Huanhuan Yu,Yuzhuo Dong,Wenjing Quan,Zhimin Su,Longyun Li
Lonicera macranthoides, the main source of traditional Chinese medicine Lonicerae Flos, is extensively cultivated in Southwest China. However, the quality ofL. macranthoidesproduced in this region significantly varies due to its wide distribution and various cultivation breeds. Herein, 50 Lonicerae Flos samples derived from different breeds ofL. macranthoidescultivated in Southwest China were collected for quality evaluation. Six organic acids and three saponin compounds were quantitatively analyzed using HPLC. Furthermore, the antioxidant activity of a portion of samples was conducted with 2,2′-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging experiments. According to the quantitative results, all samples met the quality standards outlined in the Chinese Pharmacopoeia. The samples from Guizhou, whether derived from unopened or open wild-type breeds, exhibited high quality, while the wild-type samples showed relatively significant fluctuation in quality. The samples from Chongqing and Hunan demonstrated similar quality, whereas those from Sichuan exhibited relatively lower quality. These samples demonstrated significant abilities in clearing ABTS and DPPH radicals. The relationship between HPLC chromatograms and antioxidant activity, as elucidated by multivariate analysis, indicated that chlorogenic acid, isochlorogenic acid A, isochlorogenic acid B, and isochlorogenic acid C are active components and can serve as Q-markers for quality evaluation.
研究领域:
生药学
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分析化学
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中药学
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产品关联
DOI
PubMed
高影响因子
Biocatalysis and Agricultural Biotechnology
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2024
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IF 3.4
Sethuraman Sathya,Jiarui Zhang,Huafang Feng,Pu Jing,Yu Lan,Xiaonan Cao,Qingqing Liu
Ginseng (Panax ginseng C.A. Meyer) is a widely used dietary supplement in Traditional Chinese Medicine due to its numerous health-promoting properties. However, limited research is available on the distinctions in the chemical composition between different ginsengs. Hence, this study explores the chemical composition of different ginsengs obtained from different habitats in Northeastern China. The fresh, red, and white ginsengs were collected from different places in the Jilin province, China. Ginsenoside and non-ginsenoside contents in the ginseng extracts were analyzed by an Ultra-high performance liquid chromatography system & VION® ion mobility hybrid Quadrupole Time-of-Flight. The results show a notable difference in the ginsenoside content among the samples. The qualification ratio of ginsenoside Re, Rg1, and Rb was 46.2%, with Re being the most abundant, followed by Rg1 and Rb1 in all the ginseng analyzed. Furthermore, the study revealed that white and red ginseng had higher total ginsenosides (8.18 ± 0.04 mg/kg) and total polysaccharides (17.4%) content, respectively. The fresh ginseng samples exhibited higher protein content (20.02 ± 0.2 g/100g) and lower fat content (2.0 g/100g) than others. Further, the ginseng samples analyzed in this study met the requirements specified in the Chinese Pharmacopoeia for total ash, heavy metals, and organochlorine pesticide residues. This study offers a comprehensive comparative analysis of the chemical composition of P. ginseng sourced from various habitats in Northeastern China. It underscores the significance of making informed choices when selecting ginseng types for scientific evaluation.
研究领域:
药理学
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化学
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传统中药
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植物学
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2024
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IF 8.1
Kai-Yu Ruan,Teng-Fei Lu,Jing Zhou,Xin Liang,Sheng Wang,Yu-Qian Xia,Tong Wang
The utilization of bio-derived solvents holds immense promise in the extraction of natural products. In the present study, a systematic evaluation was conducted to assess the performance of bio-derived solvents in simultaneously extracting polysaccharides and phenolic substances from Carthamus tinctorius L. flower. Among them, ethyl lactate (24.7%) exhibited superior efficacy. A comprehensive investigation was also conducted on the salt response behavior of ethyl lactate and its in-situ enrichment efficiency for polysaccharides and phenolics, demonstrating that the recovery yield, enrichment factor, and separation factor can be finely tuned by manipulating the system composition. Based on these findings, we have developed a highly efficient ultrasound-assisted method that is responsive to salt and enables simultaneous extraction and in situ enrichment of polysaccharides and phenolics. In comparison with the method in Chinese Pharmacopoeia (2020), the extraction yield of Carthamus tinctorius L. flower quality markers is increased by more than 1.14 times, while the extraction time is shortened by over 4 times. Additionally, the amount of solvent used is reduced by a factor of 5.73, eliminating the need for methanol and corrosive HCl that are harmful to the environment. The findings of this study will provide crucial theoretical guidance for the application of bio-derived solvents, demonstrating significant potential in terms of quality control and large-scale preparation of natural medicines.
研究领域:
药学
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天然产物化学
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生物技术
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环境科学
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产品关联
DOI
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 44
·5673-5679
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IF 2.378
Yang Chen,Weiman Zhao,Chen Qing,Wei Wu,Shaoyong Chen,Zijun Bie
Panax ginseng has been one of the most famous traditional herbs in eastern Asia as well as North America for thousands of years. Ginsenosides are considered to be the main bioactive constituent with diverse pharmacological functions. Thus, selective analysis of ginsenosides is of great importance for both the medical perspective and quality control of Panax ginseng. However, the conventional RPLC is not suitable for the analysis of ginsenosides because of the strong polarity of the glycosyl chain. Herein, by employing boronate affinity mesoporous silica nanoparticles (BA-MSNs) as a selective sorbent, we have developed a boronate affinity chromatography based strategy for the analysis of ginsenosides. As compared to the standard analytical approach (Chinese Pharmacopoeia 2015), the proposed method did not require tedious sample pretreatment, but provided a significantly improved performance, including excellent selectivity, high binding capacity and fast binding equilibrium. Using ginsenosides Re, Rb1 and Rb2 as representative targets, efficient qualitative and quantitative analyses of ginseng were performed, suggesting a promising outlook for the proposed method in real-world applications.
研究领域:
分析化学
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药学
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中医药
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产品关联
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2024
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IF 3.1
Jiaying Sheng,Han Gao,Mei Zhang,Danke Xu
The pollution of mycotoxins to crops such as traditional Chinese medicines (TCMs) is an established problem throughout the world. Thus, mycotoxin determination in TCMs during production and processing is significantly necessary, which means rapid, sensitive and accurate analytical methods are needed. In this work, a new method of visual protein microarray based on a 96-well microtiter plate was proposed. Combined with a colorimetric method, five mycotoxins (ochratoxin A, zearalenone, deoxynivalenol, aflatoxin B1 and fumonisin B1) in 90 samples (TCMs) could be detected simultaneously within 30 minutes. The detection limits for the five mycotoxins are 0.25 μg/kg, 0.33 μg/kg, 11.84 μg/kg, 0.06 μg/kg, and 3.58 μg/kg, which can satisfy specified requirements of mycotoxins in the Chinese Pharmacopoeia (2020 edition) adequately. Under repeated conditions, experiments were carried out on actual samples to verify the feasibility of the method. The results showed that the recoveries of all analytes were between 70 % and 120 %, and the relative standard deviations were less than 15 %. In comparison to LC-MS/MS, this method significantly reduces the required time, and the colorimetric technique offers more direct results compared to fluorescence-based screening assays. This method exhibits substantial potential for the rapid and sensitive on-site detection of TCMs for quality control.
研究领域:
分析化学
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药理学
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食品安全
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）