JOURNAL OF CHROMATOGRAPHIC SCIENCE
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2024
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IF 1.5
Zhang Chenxia,Wu Zeyi,Qiu Meiyan,Li Yanhua,Wang Zhongqing,Fan Xiaomei,Cai Shaoyu,Rao Wanbing
The difficulty on determination of high boiling point solvents, always exist due to their low vapor pressure in headspace (HS) vial. What’s worse, the commonly used GC diluents, such as N, N-dimethylacetamide, 1,3-dimethyl-2-imidazolidinone, dimethyl sulfoxide, N, N-dimethylformamide and N-methylpyrrolidone are often remained in HS instrument after frequent injection into HS, thus would result in terrible positive interference problem. In this work, a fast and sensitive HPLC method in combination with a sample precipitation method, was proposed for the determination of above five high boiling point solvents in drug substances within 14.5 min. The final method was validated with regard to limit of detection and limit of quantitation, precision, linearity and accuracy in accordance with ICH Q2 (R1). Finally, the proposed strategy was successfully applied for the analysis of high boiling point solvents used in three active pharmaceutical ingredients. The established generic strategy would provide another choice for the determination of high boiling point solvents in pharmaceuticals.
研究领域:
分析化学
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药学
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PubMed
高影响因子
Polymers
·
2020
·Vol. 12
·期 9
·1921
·
IF 3.426
Yanyan Lou,Fibor J. Tan,Rong Zeng,Mengen Wang,Pan Li,Shengji Xia
The unique two-dimensional structure and chemical properties of graphene oxide (GO) provide a convenient method for preparing novel membranes. In this study, GO membranes were prepared through filtration by a pressure-assisted self-assembly method involving the cross-linking of three diamine monomers on a polyethersulfone (PES) support. The different small molecular diamines, ethylenediamine, butanediamine, and p-phenylenediamine, were introduced as cross-linking agents to investigate the effect of diamine on the properties of GO membranes. The hydrophobic substances ibuprofen, gemfibrozil, and triclosan were selected as target pharmaceuticals and personal care products (PPCPs). The adsorption and molecular sieving activities of PPCPs by cross-linked GO membranes at a pH of 3 were investigated. The permeate water was analyzed for dissolved organic carbon, ultraviolet absorption at 254 nm, molecular weight distribution, and fluorescence excitation–emission matrices. The results showed that the removal of hydrophobic PPCPs by GO membranes was mainly due to their adsorption and molecular sieving activities. Adsorption was mainly determined by the hydrophilic and hydrophobic properties of the membranes and PPCPs. The interception effect was mainly determined by the interlayer spacing between the GO membranes and the molecular weight and steric hindrance of the PPCPs. A smaller spacing of the GO membrane layers resulted in greater steric hindrance and a higher removal rate.Keywords:graphene oxide;polyethersulfone;cross-linked;ultrafiltration membrane;PPCPs
研究领域:
材料科学
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环境科学
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PubMed
高影响因子
LANGMUIR
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2021
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IF 3.882
Xiuxiu Yang,Zhongqiang Yang
This work established a liquid crystal (LC) aptasensor for simple and rapid detection of ibuprofen, a typical pharmaceuticals and personal care products (PPCPs) pollutant. A negatively charged DNA aptamer specific for ibuprofen and a positively charged amphiphilic surfactant, hexadecyltrimethylammonium bromide (CTAB), were incubated with the sample and then directly added onto the LC interface. In the presence of ibuprofen, the specific binding of ibuprofen with the DNA aptamer will release CTAB, which then adsorbed at the LC–aqueous interface and induced the orientational change of LCs to homeotropic orientation with a dark optical signal output. While in the absence of ibuprofen, the DNA aptamer binds with CTAB through hydrophobic and electrostatic interactions, LCs remained in the planar orientation with a bright optical signal output. This LC aptasensor also has good specificity for ibuprofen and can even detect ibuprofen drug in tap water. Moreover, the response time of the LC aptasensor is fast in minutes. Additionally, this LC aptasensor benefits in monitoring the water quality and inspires the exploration of a general platform for PPCPs detection.
研究领域:
分析化学
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环境科学
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传感技术
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DOI
PubMed
高影响因子
JOURNAL OF BIOTECHNOLOGY
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2024
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IF 4.1
Hanwei Zheng,Zhiqing Liu,Hao Zheng,Yuman Miao,Chenyu Liu,Minhua Zong,Wenyong Lou
To enhance the activity of Lactiplantibacillus plantarum FS4722 ( L. plantarum FS4722 ) in reducing uric acid, this study optimized the culture medium components and fermentation conditions using response surface methodology. The results indicated that the optimal culture medium comprised glucose at 25.61 g/L, yeast extract powder ( YEP ) at 26.86 g/L, and nucleoside addition at 0.48 g/L. The optimal fermentation conditions were: a fermentation time of 10 h, an initial pH of 6.5, a fermentation temperature of 33 °C, and an inoculum size of 1.0 %. Under these conditions, the nucleoside degradation ability of L. plantarum FS4722 increased by 41.9 times, enhancing its potential applications in reducing uric acid and developing anti-gout foods, health supplements, and pharmaceuticals.
研究领域:
微生物学
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生物技术
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食品科学
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药学
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DOI
PubMed
高影响因子
MOLECULES
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2025
·Vol. 30
·期 14
·2942
·
IF 4.6
Xinyu Gao,Jiahao Niu,Zhengjian Xiao,Da Rong,Mingming Yu,Sherwin K B Sy,Cong Wang,Zhihua Lv
Melatonin supplements have been widely used to improve sleep quality and overcome sleep disorders, with melatonin and vitamin B6 serving as the primary active ingredients. This study developed a novel analytical method for the simultaneous quantification of melatonin and vitamin B6 using 1 H-NMR spectroscopy. The characteristic signals of melatonin and vitamin B6 hydrochloride at δ 7.09 ppm and δ 8.12 ppm were selected for quantitative analysis, with maleic acid used as the internal standard. The method was validated for specificity, precision, and stability. The results demonstrate that the method exhibits high precision and complies with the guidelines established by the China Food and Drug Administration (CFDA). Furthermore, this method has been successfully applied to commercially available formulations. Compared to conventional methods, the 1 H-NMR technique offers a more efficient and simpler alternative, making it suitable for the simultaneous quantitative determination of melatonin and vitamin B6 hydrochloride. This approach ensures the quality, stability, and safety of commercial melatonin products.
研究领域:
分析化学
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药物分析
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核磁共振（NMR）谱学
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质量控制
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PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Huiming Qi,Li Zhang,Mingchao Zhang,Liya Ma,Qiang Gao,Fuzhen Jiang,Delin Qi,Shubo Deng,Wei Wang
Understanding the molecular factors that govern the adsorption of pharmaceutical and personal care products (PPCPs) on carbon nanotubes (CNTs) is essential for designing highly efficient porous adsorbents. We hypothesize that adsorbent pore and adsorbate size matching is the dominant factor controlling the adsorption process. Therefore, this study examined the effects of the molecular size of PPCPs and pore size of CNTs on the adsorption performance using the combination of experiments and molecular dynamics simulations. The results show that the adsorption capacity of unit specific surface area increased with increasing both the molecular size of PPCPs and the pore size of CNTs. In the coexisting system, adsorption capacity declined with decreasing molecular size, showing negative correlation (R²=0.842). This indicates that small PPCPs are more vulnerable to competitive inhibition, whereas larger molecules exhibit stronger resistance to competition and maintain more stable adsorption capacities in complex media. Molecular dynamics simulation revealed that the interaction between CNTs and PPCPs was mainly dominated by van der Waals forces, with larger-sized molecules showing higher binding energy and being more likely to occupy adsorption sites on CNTs. These findings not only reveal the internal relationship between the pore size of sorbents and their adsorption capacity, but also provide an important theoretical basis and practical guidance for optimizing PPCPs removal strategies.
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高影响因子
BIORESOURCE TECHNOLOGY
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2020
·
IF 7.539
Mei Chen,Lehui Ren,Kangquan Qi,Qiang Li,Miaoju Lai,Yang Li,Xuesong Li,Zhiwei Wang
A novel electrochemical membrane bioreactor (EMBR) was developed for enhancing PPCPs removal from real municipal wastewater. Compared to the control MBR (CMBR) without applying electric field, EMBR exhibited higher removal efficiencies for 14 PPCPs among all investigated PPCPs, including 3 fluoroquinolones, 2 macrolides, 6 sulfonamides and 3 anti-inflammatory drugs, while no significant difference was observed for the rest 8 PPCPs. The enhanced removal of 14 PPCPs was mainly attributed to electrooxidation by the direct anodic oxidation and reactive oxygen species-mediated indirect oxidation. Moreover, membrane fouling rates of EMBR (0.55 ± 0.10 kPa/d) were significantly reduced compared with CMBR (0.99 ± 0.09 kPa/d). Microbial activities and community analyses demonstrated that the applied electric field had no noticeable adverse impact on microbial viabilities, richness and diversity. These findings demonstrated that this EMBR enhanced pollutant removal and mitigated membrane fouling simultaneously, highlighting the potential of the novel technology to be used for removing PPCPs from wastewater.
研究领域:
环境工程
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水处理技术
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电化学
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微生物学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
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2024
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IF 6.1
Mingsan Miao,Liang Guo,Jinyan Xue,Yuzhen Jia,Zhenzhen Cui,Huai-Xia Yang
The development of a simple, rapid, and sensitive technology for the simultaneous detection of mycotoxins is of great significance in ensuring the safety of foods and drugs. Herein, a fluorescence aptasensor with high sensitivity and reproducibility for the simultaneous detection of aflatoxin B1 (AFB1) and ochratoxin A (OTA) was developed. In this sensing system, AFB1 and OTA aptamers were co-immobilized on the surface of magnetic beads (MBs) to form a Y-shaped structure through the principle of complementary base pairing, which were used as recognition probes to specifically capture the target. Activators generated by electron transfer for atom transfer radical polymerization (ARGET ATRP) was used as signal amplification strategy to improve the sensitivity. The initiator modified at the end of the antibody initiates the ARGET ATRP reaction. Different fluorescence signals were designed to achieve simultaneous detection of OTA and AFB1 with the limit of 18.62 and 1.60 fg/mL for AFB1 and OTA, respectively. In addition, experiments were conducted on three types of samples, and the recoveries of the two mycotoxins ranged from 87.30% to 109.50%, with relative standard deviations ranging from 0.50% to 4.92% under reproducible conditions. The results suggest that the developed aptasensor is sufficient to meet the different regulatory requirements of the two mycotoxins in food and drug safety and shows great potential.
研究领域:
分析化学
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生物技术
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食品科学
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毒理学
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DOI
PubMed
高影响因子
AQUATIC TOXICOLOGY
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2021
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IF 4.964
Naima Hamid,Muhammad Junaid,Rakia Manzoor,Jin-Jing Duan,Ming Lv,Nan Xu,De-Sheng Pei
Pharmaceuticals and personal care products (PPCPs) as emerging contaminants are ubiquitously present in the aquatic environment. Using in vivo and in silico techniques, this study aims to elucidate tissue distribution and endocrine disruption effects of chronic exposure (120 days) to PPCP mixture at environmentally relevant concentrations (ERCs) in adult zebrafish. Results from UHPLC-MS/MS analyses showed elevated distribution of PPCPs in zebrafish tissues in the order of liver > gonad > brain. Upregulation of steroid hormone receptors, both gonadotropin, and steroidogenic genes perturb the HPG axis pathway in females, while male fish exhibited significantly downregulated expressions of vtg, cyp17 , and 17βhsd genes with inhibited fecundity. The Spearman correlation indicated a significant positive relationship between PPCPs bioaccumulation and mRNA levels of HPG axis genes. In silico molecular docking (MD) revealed specific amino acid residues of PPCPs binding with zebrafish estrogen receptors. Furthermore, the strongest binding energies of sulfamethoxazole, carbamazepine, and triclosan were discovered in erα and erβ estrogen receptors, confirming PPCPs' xenoestrogenic behavior. To summarize, chronic exposure to ERCs resulted in a high accumulation of PPCPs in the liver and gonad tissues of adult zebrafish, as well as associated perturbed genetic responses. As a result, strict environmental regulations for the disposal of PPCPs should be ensured to protect ecological and public health.
研究领域:
环境科学
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毒理学
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水生生物学
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分子生物学
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DOI
PubMed
高影响因子
ADVANCED COMPOSITE MATERIALS
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2025
·
IF 2.3
Zhiwei Cai,Jing Sun,Ning Jiang,Chao Chen,Yanzi Lin,Yimin Shen,Minmin Ge,Guanglin Fang,Corinne Boudon,Yaokang Lv,Laurent Ruhlmann
Novel Ce/TiO2 were synthesized using a solution-based method involving two types of Ce(III)-doped polyoxotitanium cages, namely ([Ti28O38(OEt)38CeCl(EtOH)1.4] and [Ti8O7(OEt)21Ce(EtOH)]). This novel material was used to coat the surface of polyvinylidene chloride (PVDC)/polyvinyl chloride (PVC) film, resulting in a homogeneous CeO2/TiO2@PVDC/PVC film. Remarkably, the CeO2/TiO2@PVDC/PVC film exhibited notable hydrophobicity and UV absorption capacity. Furthermore, the UV absorption capacity of the films was significantly improved with increasing CeO2/TiO2 content. The incorporation of CeO2/TiO2 also led to a substantial improvement in the barrier properties of the film, particularly in terms of water vapor permeability. These modified films hold great potential and offer a wide range of applications in the pharmaceutical packaging and food packaging industries.
研究领域:
材料科学
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纳米技术
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高分子科学
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包装工程
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DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
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2025
·
IF 21.1
Shuyu Qin,Haoyi Wu,Junhao Peng,Yuanmin Zhu,Huafeng Dong,Wei Zhang
The contamination of wastewater by pharmaceuticals and personal care products (PPCPs) poses a significant threat to environmental and human health, necessitating innovative and efficient purification strategies. Photocatalysis is a promising approach for PPCPs degradation, however, optimizing solid-liquid interfacial adsorption and charge transfer remains a major challenge. Here, we introduce a novel europium (Eu) single-atom anchored TiO 2 photocatalyst, which simultaneously enhances adsorption capacity, light absorption range, and charge transfer efficiency, leading to unprecedented photocatalytic performance. Experimental and theoretical investigations reveal that Eu decoration lowers the adsorption potential of TiO 2 , significantly improving its ability to adsorb O 2 , H 2 O, and pharmaceuticals, the typical PPCPs. The introduction of 5d electrons from Eu creates a donor level below the conduction band, extending TiO 2 ’s absorption from the ultraviolet to the visible spectrum while improving adsorption and accelerating charge transfer at the solid-liquid interface. The yield of superoxide radicals rises significantly, while the reaction probability for pharmaceutical molecules is raised through the strong surface attraction. Consequently, the pharmaceutical-degradation efficiency increases by up to 27-fold under visible light and 3.9-fold under UV irradiation. This work presents a paradigm shift in photocatalytic water purification, offering a scalable and efficient strategy for tackling PPCPs contamination in wastewater. By unveiling a new mechanism for single-atom-enhanced photocatalysis, this study provides critical insights into the design of next-generation catalysts for sustainable water treatment.
研究领域:
环境科学
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材料化学
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光催化
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水净化
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纳米技术
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产品关联
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Xin Wang,Jingyang Wang,Yu Luo,Weiye Xiu,Shiyou Yu,Mengyuan Yang,Kechi Zhou,Yongqiang Ma
Fluorescein isothiocyanate (FITC) was used for the fluorescent labeling of sweet corn cob polysaccharide (SCP), followed by identification using fluorescence spectroscopy, UV–Visible spectroscopy, infrared spectroscopy, and NMR analysis. The results confirmed the successful attachment of FITC to SCP with a substitution degree of 1.28%. This established fluorescent sweet corn cob polysaccharide (FSCP) was then subjected to an in vivo quantitative analysis, in which mice were administered with FSCP, fluorescent sweet corn cob polysaccharide nanoemulsion (FSCP-NE), and fluorescent sweet corn cob polysaccharide nano emulsion microcapsule (FSCP-NE-ME) via gavage followed by investigating the in vivo plasma pharmacokinetics and tissue distribution. The results revealed that FSCP-NE could improve the utilization efficiency of SCP, and the FSCP-NE microcapsules exhibited good controlled-release performance, which significantly improved the utilization efficiency of SCP. The absorption and metabolism of SCP in vivo were explored next, which provided the reference for future research and development on SCP for application in the fields of nutraceuticals and pharmaceuticals.
研究领域:
药理学
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纳米技术
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生物技术
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食品科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）