MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 9
·1-7
·
IF 5.479
Zhang Pu,Jia Chunyan,Zhao Yannan,Luo Honghong,Tan Xin,Ma Xiaohong,Wang Yi
Tiopronin is a widely used drug for treatment of cystinuria, rheumatoid arthritis and hepatic disorders. It is also an antidote to heavy metal poisoning and a radioprotective agent. A method is described for rapid and sensitive determination of tiopronin using DNA-stabilized silver nanoclusters (DNA–AgNCs) as a fluorescent probe. Tiopronin can selectively bind to DNA–AgNCs to form a stable Ag-S bond upon which the red photoluminescence (best measured at excitation/emission wavelengths of 590/640 nm) is quenched. The finding is used to design an assay that has a linear response in the 1–150 nM tiopronin concentration range and a 270 pM limit of detection. Compared with previously reported methods, the present approach is more rapid, highly sensitive and selective. It has been successfully applied in the detection of tiopronin in spiked urine and serum, and in pharmaceutical products (tablets and injections).
An ultrasensitive and reliable method for tiopronin assay is developed using red-emissive silver nanoclusters as a fluorescent probe. It has been successfully applied in the determination of tiopronin in biological fluids and pharmaceutical products.
研究领域:
分析化学
·
生物医学
·
药学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
·
2020
·Vol. 31
·期 10
·2120-2128
·
IF 1.399
Ge Dandan,Gao Ying,Cao Yunxin,Dai Enrui,Yuan Lin
A vortex-assisted liquid-liquid microextraction method combined with high performance liquid chromatography-diode array detector has been developed for the determination of parabens in foods, cosmetics and pharmaceutical products. In this work, polymeric deep eutectic solvents composed of DL-menthol and polyethylene glycol with three different light grades were prepared successfully for the first time and used as extraction solvents. The influencing parameters on the extraction efficiency including type of extraction solvent, salt addition, composition of deep eutectic solvent, volume of extraction solvent, extraction time and solution pH were evaluated. Under the optimized conditions, using DL-menthol and polyethylene glycol 400 at a molar ratio of 1:1 as the extraction solvent, the method exhibited good linearity with linear coefficients greater than 0.9995. Limits of detection and limits of quantification were in the range of 0.3-2 ng mL-1 and 1-5 ng mL-1, respectively. The proposed method was successfully applied to determine four parabens in seven commercial products. The proposed method is simple, green and efficient, and could be applied to determine parabens in complex matrices.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Analysis and Testing
·
2021
·Vol. 5
·期 3
·210-216
·
IF 0
Liu Chun,Pang Qiwei,Wu Tong,Qi Wenjing,Fu Wensheng,Wang Yi
A rapid and sensitive colorimetric detection of ascorbic acid (AA) through the morphology transformation of silver triangular nanoplates (Ag TNPs) is developed. By virtue of the redox reaction among silver nitrate and AA, the newly formed Ag atoms deposited on the surfaces of Ag TNPs. Subsequently, the morphology of Ag TNPs transforms from triangle to circle, resulting in a more than 160 nm blue shift of localized surface plasmon resonance absorption peak. The corresponding color of the solution converting from blue to yellow with the concentration of AA can be observed by naked eyes within 15 min. A linear relationship between the blue shift of absorption peak and the concentration of AA ranging from 0.2 to 6 μM is obtained with a limit of detection (LOD) of 100 nM (3 σ ). Some potential species (e.g., glucose, urea and various amino acids) coexisting in the system showed little or no interference. The proposed assay is successfully employed to determine the amount of AA in pharmaceutical products with recoveries from 96.9 to 106.5% and offers a sensitive, low-cost, rapid and simple assay of visual analysis of ascorbic acid.
研究领域:
分析化学
·
纳米技术
·
药学
✓ 已验证
产品关联
DOI
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Jiaping Liu,Yaqiong Zhang,Songzhe He,Aimei Zhou,Boyan Gao,Mengyu Yan,Liangli (Lucy) Yu
In this study, a microbial transglutaminase (MTGase)-catalyzed reaction was first optimized to prepare the cross-linked sodium caseinate (MSC). At the optimal condition of MTGase to sodium caseinate mass ratio of 250 U/g and 2 h of reaction time, different concentrations of sodium caseinate (5, 25 and 50 mg/mL) were used to synthesize MTGase-catalyzed cross-linked sodium caseinate (named as MSC-5, MSC-25 and MSC-50, respectively), which were further characterized using fourier transform infrared spectroscopy (FTIR), gel permeation chromatography (GPC), circular dichroism spectroscopy (CD) and scanning electron microscopy (SEM). Compared to those of sodium caseinate, MSC exhibited a higher molecular weight and surface hydrophobicity index, different secondary structure and microstructure characteristics. Then, MSC-50 was used as the coating stabilizer for zein nanoparticles and their composite nanoparticles (zein-MSC-50 NPs) exhibited a spherical shape with an average diameter of 240.70 nm, a narrow size distribution and negative surface charge of −23.83 mV at the optimized zein to MSC-50 mass ratio of 1:2. The stability studies indicated that zein-MSC-50 NPs had desirable re-dispersibility, thermal, salt and storage stabilities. These results suggested a possible utilization of MSC as the novel stabilizer of zein-based delivery carriers for encapsulating lipophilic bioactive components in functional foods and pharmaceutical products.
研究领域:
食品科学
·
纳米技术
·
生物技术
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）