OPTICS AND SPECTROSCOPY
·
2013
·Vol. 115
·期 4
·596-600
·
IF 0.559
Wang Rui-yong,Wu Jing,Wang Lv-jing,Wang Rui,Dou Huan-jing
A novel CdTe quantum dots (QDs) based technology platform was established in aqueous solution. It can perform accurate and simple determination of aminophylline concentration in pharmaceutical samples with satisfactory results. Under optimum conditions, the relative fluorescence intensity of CdTe quantum dots is linearly proportional aminophylline concentration from 2.00 to 80.0 μg mL −1 with a correlation coefficient of 0.9979 for aminophylline determination and a detection limit of 0.531 μg mL −1 .
研究领域:
分析化学
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药剂学
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纳米医学
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药物分析
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生物传感
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DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 22
·7761
·
IF 4.927
Yuhong Li,Jianxiong Tang,Yueli Lin,Jiejun Li,Yaqi Yang,Pengcheng Zhao,Junjie Fei,Yixi Xie
Rutin, a natural flavonol glycoside, is widely present in plants and foods, such as black tea and wheat tea. The antioxidant and anti-inflammatory effects of flavonoids are well known. In this study, a new electrochemical rutin sensor was developed using multiwalled carbon nanotubes/aluminum-based metal–organic frameworks (MWCNT/CAU-1) (CAU−1, a type of Al-MOF) as the electrode modification material. The suspension of multiwalled carbon tubes was dropped on the surface of the GCE electrode to make MWCNT/GCEs, and CAU−1 was then attached to the electrode surface by electrodeposition. MWCNTs and CAU−1 were characterized using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). Due to the synergistic effect of CAU−1 and MWCNT-COOH, the prepared sensor showed an ultrasensitive electrochemical response to rutin. Under optimized conditions, the sensor showed a linear relationship between 1.0 × 10−9~3.0 × 10−6M with a detection limit of 6.7 × 10−10M (S/N = 3). The sensor also showed satisfactory stability and accuracy in the detection of real samples.Keywords:electroanalysis;pyrolysis;carbon materials;drug analysis;modified electrode
研究领域:
分析化学
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材料科学
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电化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF LUMINESCENCE
·
2013
·
IF 2.144
Suyan Ying,Shumin Cui,Weiping Wang,Jiuju Feng,Jianrong Chen
A simple and sensitive method for detecting diprophylline (DPP) was developed based on the fluorescence quenching of glutathione-capped CdTe quantum dots (GSH–CdTe QDs) by using diprophylline in a KH 2 PO 4 –Na 2 HPO 4 medium. Parameters affecting the quenching efficiency, including types and pH of buffer solutions as well as temperature, reaction time, adding sequence, and interfering substances, were investigated and optimized. In optimum conditions, the calibration plot of the quenched fluorescence intensity F 0 / F with a DPP concentration range of 1.67×10 –6 mol L −1 to 1.33×10 –5 mol L −1 was linear. The detection limit (with signal to noise ratio of 3) for DPP was 2.24×10 –7 mol L −1 . The proposed method was successfully applied for detecting DPP in human serum. The recovery of the method was in the range of 87.41% to 117.94%. Finally, the possible quenching mechanism of GSH–CdTe QDs and DPP was also discussed.
研究领域:
分析化学
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药学
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生物医学工程
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产品关联
DOI
高影响因子
RUSSIAN JOURNAL OF GENERAL CHEMISTRY
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2022
·Vol. 91
·期 1
·S137
·
IF 0.779
Zhu Xue,Li Mingtong,Wang Ruiyong,Tian Fengshou,Chen Yahong
Abstract In this study, the influencing factors of Hantzsch reaction have been investigated and a simple spectrofluorimetric method for the determination of acyclovir, ganciclovir, famciclovir, oxiracetam, and prazosin hydrochloride developed. The proposed method is based on the Hantzsch reaction of acetyl acetone-formaldehyde reagent with drugs. The results indicate that the drugs fluorescence in H 2 O is inhibited and enhanced in DMSO.
研究领域:
药学
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分析化学
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DOI
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 6
·1343
·
IF 4.6
Yiaobo Zhang,Lin Liu,Jiahui He,Chengzhi Huang,Lei Zhan,Chunmei Li
The development of sensitive and specific methods for the high-quality analysis of hemin-related drugs is significant in the pharmaceutical field. In this work, a simple and rapid method based on the fluorescent properties of carbon dots (CDs) was established for the determination of hemin in drugs. By taking melamine and ethylenediamine as the reaction materials, the fluorescent CDs were synthesized by a one-step solvothermal method, which can be used for the determination of hemin in drugs by the fluorescent inner filter effect. The as-prepared fluorescent CDs with rich functional groups on the surface displayed good water solubility, strong salt resistance, robust pH stability, and photobleaching resistance. Most importantly, the fluorescent excitation wavelength of fluorescent CDs was very close to the absorption wavelength of hemin, providing the evidence for the fluorescent inner filter effect. When the hemin concentration was in the range of 0.01–1 μM, there was a good linear relationship between the hemin content with the fluorescence intensity of CDs. The linear regression equation was (1 −F/F0) = 0.0897c+ 0.0124, with a correlation coefficient (R2) of 0.9982 and a detection limit of 9 nM. This assay was successfully used to determine the content of hemin in the tablet, which displayed 97.9–105.5% of the labelled amount, with a relative standard deviation of less than 3%. The developed fluorescence method for the detection of hemin content displays the advantages of accurate, rapid, and high sensitivity, which could prove to be a useful tool for the determination of hemin supplement tablets.
研究领域:
药学
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分析化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2014
·Vol. 30
·期 6
·905-909
·
IF 1.119
Zhang Jinlei,Lan Cuiling,Shi Bingfang,Liu Fang,Zhao Dandan,Tan Xuecai
A novel type of carboxylated multiwalled carbon nanotube modified electrode(c-MWCNTs/GCE) was constructed and the electrochemical properties of phenacetin(PHE) at it were studied. In a buffer solution of 0.1 mol/L HAc-NaAc(pH=5.3), PHE exhibited a couple of quasi-reversible redox peaks and an anodic peak in the potential range of 0.2–1.2 V at c-MWCNTs/GCE. The peak current was proportional to the concentration of PHE in the range of 4.0×10 −6 –1.0×10 −4 mol/L with a detection limit of 1.0×10 −6 mol/L(S/N=3). The c-MWCNTs/GCE showed excellent repeatability and stability and the electrochemical reaction mechanism of PHE was proposed. This method was used to determine the content of PHE in medical tablets and the recovery was determined to be 96.5%–104.2% by means of a standard addition method.
研究领域:
分析化学
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药学
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材料科学
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产品关联
DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 11
·6075-6082
·
IF 2.936
Qing Liu,Zhihai Xie,Tao Liu,Jin Fan
The interaction of silica nanoparticles (SiO2NPs) with berberine hydrochloride (BRH) was studied in aqueous solution at pH 9.0 and room temperature by using fluorophotometry. Based on a significant enhancement of the fluorescence intensity of the SiO2NPs–BRH aggregates, a spectrofluorimetric method which was simple, sensitive and green was developed for the determination of BRH in aqueous solution. The linear range of the method was from 2.0–50.0 μg L−1 with a detection limit of 0.73 μg L−1. There was no interference from the compounds normally used to formulate pharmaceutical tablets. The proposed method was applied to the determination of BRH in tablets with satisfactory results and good consistency with the results obtained by standard methods.
研究领域:
分析化学
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药学
·
纳米技术
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DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Ying Zou,Chaoxian Chi,Fangling Wu,Chuan-Fan Ding
Retinoic acid (RA) regulates cell proliferation, differentiation, and apoptosis and exists in three primary isomers: all-trans-RA (ATRA), 9-cis-RA (9cRA), and 13-cis-RA (13cRA). RA derivatives, such as bexarotene (BT) and arotinoid acid (TTNPB), are used to treat cutaneous T-cell lymphoma and induce cellular pluripotency. Due to their distinct biological activities, accurate identification and analysis of these isomers are crucial. This study developed a rapid and efficient method for analyzing RA isomers and their derivatives using ion mobility mass spectrometry (IM-MS) and theoretical calculations. RA isomers and derivatives exhibit overlapping mobilities and cannot be separated by direct IM-MS. By forming ternary complexes with β-cyclodextrin (β-CD) and metal ions, baseline separation was achieved with a peak resolution (R p-p ) of up to 3.42. Tandem MS and theoretical calculations revealed distinct differences in interactions, spatial structures, and binding energies, enabling mobility-based separation. Photostability tests showed rapid photoisomerization of 13cRA into 9cRA and ATRA within seconds. Analysis of 13cRA soft capsules from four brands demonstrated good stability, with measured content aligning closely with labeled values. This method offers a new approach for accurate RA isomer analysis, with potential applications in pharmaceuticals and food industries.
研究领域:
生物化学
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药物化学
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分析化学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2017
·
IF 3.981
Nadeem Muhammad,Qamar Subhani,Fenglian Wang,Dandan Guo,Qiming Zhao,Shuchao Wu,Yan Zhu
This work illustrates the introduction of a simple, rugged and flexible column-switching ion chromatography (IC) technique for an automated on-line QuEChERS extracted samples extracts washing followed by sensitive fluorescence (FLD) determination of five acidic pharmaceutical drugs namely; clofibric acid (CLO), ibuprofen (IBU), aspirin (ASP), naproxen (NAP) and flurobrofen (FLU) in three complex samples (spinach, apple and hospital sewage sludge). An old anion exchange column IonPac ® AS11-HC was utilized as a pre-treatment column for on-line washing of inorganic and organic interferences followed by isocratic separation of five acidic drugs with another anion exchange IonPac ® AS12A analytical column by exploiting the column-switching technique. This novel method exhibited good linearity with correlation coefficients (r 2 ) for all drugs were in the range 0.976–0.996. The limit of detection and quantification of all five acidic drugs were in the range 0.024 μg/kg to 8.70 μg/kg and 0.082 μg/kg to 0.029 mg/kg, respectively, and better recoveries in the range 81.17–112.5% with percentage relative standard deviations (RSDs) less than 17.8% were obtained. This on-line sample pre-treatment method showed minimum matrix effect in the range of 0.87–1.25 except for aspirin. This simple rugged and flexible column-switching system required only 28 min for maximum elimination of matrices and interferences in three complex samples extracts, isocratic separation of five acidic drugs and for the continuous regeneration of pre-treatment column prior to every subsequent analysis. Finally, this simple automated IC system was appeared so rugged and flexible, which can eliminate and wash out most of interference, impurities and matrices in complex samples, simply by adjusting the NaOH and acetonitrile concentration in washing mobile phase with maximum recoveries of acidic analytes of interest.
研究领域:
分析化学
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环境科学
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药学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2016
·
IF 4.035
Jing Chen,Bao Qiong Li,Min Li Xu,Xue Wang,Yu Hong Jing,Hong Lin Zhai
The interference signals and overlapped peaks are common phenomena in fluorescence determination, which seriously influence the accuracy and reliability of analytical results. In this paper, Krawtchouk image moment method was introduced to the analysis of fluorescence three-dimensional (3D) spectra and applied to the quantitative analysis of three drugs including nicotinic acid, metoprolol and amlodipine in human plasma. Without any pretreatment to the obtained spectra, Krawtchouk moments were directly calculated on the grayscale images of 3D spectra, and the quantitative linear models for the three drugs were established by stepwise regression, respectively. The determination coefficients ( R ) were more than 0.9906. The correlation coefficients of leave-one-out cross-validation ( R cv ) were more than 0.9256. The precisions (RSD, %) of inter-day and intra-day variations were less than 8.4% and 3.1%, separately. The recovery was from 101.84% to 107.88%. The limit of quantification and the limit of detection were 0.12 μg mL −1 and 0.43 μg mL −1 , respectively. All the statistical parameters supported that the obtained models performed well and the proposed method was accurate and reliable. Our study indicates that Krawtchouk image moments with the powerful abilities of multi-resolution and extracting local information can be applied to the simultaneous determination of multi-target compounds in plasma based on fluorescence 3D spectra.
研究领域:
分析化学
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药理学
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医学检测
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产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2023
·
IF 4.1
Zhenkun Mao,Jinxiu Chen,Dandan Jiang,Ningmin Zhao,Yinhui Qin,Xiangju Mao,Fengqin Fang,Peizhi Ma
Itaconic acid is an excellent hydrophilic monomer owing to the dicarboxylic group possessing strong polarity. This study reports on the preparation of a new organic-polymer monolithic column poly(itaconic acid-co-3-(acryloyloxy)-2-hydroxypropyl methacrylate) (poly(IA-co-AHM)) featuring excellent hydrophilic chromatography ability and its application in pharmaceutical analysis. The monolithic column was successfully synthesized by using the monomer itaconic acid and the cross-linker AHM through an in situ copolymerization method. Optical microscopy, scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR) were employed for the characterization of the poly(IA-co-AHM) monolithic column, and all of these demonstrated that the prepared itaconic acid-based monolithic column exhibited satisfactory permeability and a homogeneous porous structure. Owing to the carboxylic groups of itaconic acid, a cathodic electroosmotic flow (EOF) was generated on the itaconic acid-based monolithic column among the pH ranges of the mobile phase from 4.0 to 9.0. Depending on the powerful hydrophilic interactions, different kinds of polar substances, including thioureas, nucleoside drugs, sulfonamides, and polypeptides, were separated efficiently by the itaconic acid-based monoliths poly(IA-co-AHM). The separations of polar compounds were successfully realized, even at a lower level of 50% acetonitrile content on this monolithic column. The highest column efficiencies corresponding to N,N′-dimethylthiourea and idoxuridine were 102 720 and 124 267 N/m, respectively. The poly(IA-co-AHM) monolithic column displayed excellent repeatability, whose relative standard deviations (RSDs) of the retention time and peak area were both lower than 5.0%. All experimental results demonstrated that the new itaconic acid-functionalized monolithic column was greatly appropriate to separate the polar compounds under the HILIC mode.
研究领域:
分析化学
·
制药工程
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2012
·
IF 3.898
Jinyun Peng,Chuantao Hou,Xiaoya Hu
An electrochemical method has been successfully demonstrated for sensitive determination of Metronidazole (MTZ) with graphene (Gr)-room temperature ionic liquid (IL) of 1-butyl-3-methylimidazolium hexafluorophosphate (BMIMPF 6 ) composite modified glassy carbon electrode (GCE). The cyclic voltammetric results indicate that Gr-IL/GCE can remarkably enhance electrocatalytic activity towards the reduction of MTZ in neutral solutions. MTZ produce a cathodic peak at about −0.69 V at this electrode. The electrochemical parameters of MTZ in the composite film were carefully calculated with the results of the charge transfer coefficient ( α ) as 0.625 and the number of electron transferred ( n ) as 4. The electrocatalytic behavior was further exploited as a sensitive detection scheme for the MTZ determination by differential-pulse voltammetry (DPV). Under optimized conditions, the concentration range and detection limit were 1.0 × 10 −7 –2.5 × 10 −5 mol L −1 and 4.7 × 10 −8 mol L −1 ( S / N = 3), respectively for MTZ. The method was successfully applied assay of the drug in the pharmaceutical dosage forms .
研究领域:
分析化学
·
电化学
·
药物分析
·
生物医学工程
·
药剂学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）