JOURNAL OF CHROMATOGRAPHY A
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2020
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IF 4.049
Qidan Cai,Huaidong Peng,Jiang Meng,Youshao Yan,Yu Zeng,Paul C.H. Li,Yue Sun
In this study, a three-phase laminar flow microfluidic chip (TPL chip) combined with HPLC was developed for monitoring free and total concentrations of paclitaxel (PTX) in blood simultaneously. A diluted whole blood sample (aqueous phase) was introduced into the chip, ethyl acetate (organic phase) was introduced into the chip for extraction, and an interphase was used to prevent the blood sample from coming into direct contact with the organic phase. Because only free drug can quantitatively diffuse into the organic extraction phase and the free drug fraction has a linear relationship with the dilution factor of blood, both the free and total drug concentrations can be obtained by detecting the concentration of paclitaxel in the organic extraction phase. The governing factor such as flow rate for extraction was optimized. Docetaxel was used as an internal standard. The reliability of the quantitative diffusion of molecules in the TPL chip was proved by the methodological investigation of PTX in PBS sample, which showed a good linearity in the concentration range of 0.5 - 100 µg/mL and a detection limit of 7 ng/mL. Good repeatibilities for retention time (RSD of PTX is 1.23%, docetaxel is 1.14%, n = 5) and peak area ratio of PTX to docetaxel (RSD is 4.38%) were obtained. For blood sample analysis, only 100 µL of sample was needed and whole pretreatment was finished in 35 min, and a recovery of 94~117% were obtained. The provided method showed advantages in fast analysis speed, minimum sample handing, and potential ability of automation, and integration.
研究领域:
分析化学
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药代动力学
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生物医学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2024
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IF 3.1
Shiqi Wang,Jiahui Wu,Qingcheng Wang,Yunfeng Zhang,Hong Yuan,Jianding Wang,Yonghui Wu,Yaxi Xu,Na Ji,Bin Quan,Haixing Wang,Qing Shen
A miniature mass spectrometer (mMS) based point-of-care testing (POCT) method was evaluated for on-site detecting the hypertension drugs, amlodipine and benazepril . The instrument parameters, including voltage, ISO1, ISO2, and CID , were optimized, under which the target compounds could be well detected in MS 2 . When these two drugs were injected simultaneously, the mutual ionization inhibition and mutual reduction between amlodipine and benazepril were evaluated. This phenomenon was severe on the precursor ions but had a small impact on the product ions, thus making this POCT method suitable for analysis using product ions. Finally, the method was validated and applied. The blood samples from patients were tested one hour after oral administration of the drugs (20 mg), and the benazepril was quantitatively analyzed using a standard curve, with detected concentrations ranging from 190.6 to 210 μg L −1 and a relative standard deviation (RSD) of 8.6 %. In summary, amlodipine has low sensitivity and can only be detected at higher concentrations, while benazepril has high sensitivity, good linearity, and even meets semi-quantitative requirements. The research results of this study are of great clinical significance for monitoring blood drug concentrations during hypertension medication, predicting drug efficacy , and customizing individualized medication plans.
研究领域:
分析化学
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临床医学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2024
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IF 8.3
Meng-Nan Liu,Ting Chen,Fang Yin,Wei Zhi Song,Lin-Xin Wu,Jun Zhang,Seeram Ramakrishna,Yun-Ze Long
For chronic wounds, frequent replacement of bandages not only increases the likelihood of secondary damage and the risk of cross infection but also wastes medication. Therefore, in situ real-time monitoring of the concentrations of residual drugs in bandages is crucial. Here, we propose a novel strategy that combines a triboelectric nanogenerator (TENG) with medical bandages to develop a smart bandage based on zeolite imidazolate framework TENG. During the process of wound healing, the electrical output of TENG changes with the continuous release of drugs. Based on the correlation between the electrical signal of TENG and drug concentration, the concentration of residual drugs in the bandage can be monitored in real-time in situ, guiding medical staff to replace the bandage at the most appropriate time. The smart bandage based on TENG provides a new strategy for in situ real-time monitoring of drug concentration and also provides an ideal and feasible solution for the field of biomedical drug sensing.
研究领域:
生物医学工程
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材料科学
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医学传感
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Pharmacogenomics & Personalized Medicine
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2021
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IF 3.912
Xikun Wu,Weichong Dong,Haoran Li,Xiuling Yang,Yiran Jin,Zhiqing Zhang,Ye Jiang
Purpose To perform therapeutic drug monitoring (TDM) of total and free plasma valproic acid (VPA) concentrations in pediatric patients with epilepsy and to analyze related factors.Patients and Methods Pediatric epileptic patients treated in 2015–2019 in our hospital were assessed. Total and free plasma VPA concentrations were obtained by UPLC and LC-MS/MS, respectively. Regression analysis was performed to examine the associations of free plasma VPA with total plasma VPA and plasma protein binding rate. The impacts of individual situation, CYP2C9 genotype, and drug combination on VPA concentration were examined.Results Of the 251 patients, 81 had lower total concentrations than effective therapeutic levels; 86 and 31 patients had infections and central nervous system dysplasia, respectively. VPA’s daily doses and free drug concentrations were significantly lower in the CYP2C9 *3/*3 genotype group versus the CYP2C9 *1/*3 and CYP2C9 *1/*1 groups (P<0.05). Free and total VPA concentrations were linked by Y = 0.0004 X2 + 0.042 X + 0.3035 (r=0.6981); VPA plasma protein binding rate and free VPA concentration were related by Y = 0.0003 X2 - 0.0127 X + 0.9777 (r=0.8136). Both total and free VPA concentrations were significantly decreased in patients simultaneously administered phenobarbital, meropenem and biapenem (P<0.05), with therapeutic failure after meropenem/biapenem co-administration.Conclusion Free VPA amounts have nonlinear relationships with total VPA amounts and plasma protein binding rate in epileptic children. Additionally, CYP2C9 *3/*3 expression affects VPA metabolism. Since phenobarbital affects VPA metabolism, TDM is recommended. Meanwhile, carbapenem-co-administration with VPA should be prohibited.
研究领域:
医学
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药理学
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遗传学
✓ 已验证
DOI
高影响因子
XENOBIOTICA
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2025
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IF 1.2
Shanshan Chen,Donghan Wang,Yuanyuan Zhao,Yaqi Sun,Yueyao Luan,Qixuan Sun,Jiaqi Wang,Yuhang Yan,Jing Yu,Chunhua Zhou
With the increasing prevalence and escalating complexity of mental disorders, precise medication has become critically important. This necessitates an efficient, accurate, and convenient method for drug concentration monitoring to support laboratory personnel and clinicians. In this study, three liquid chromatography-tandem mass spectrometry methods were developed and validated for simultaneously determining and quantifying 27 antipsychotics and related metabolites in human plasma. The plasma samples were subjected to protein precipitation using methanol, with isotope-labelled internal standards (ISs), followed by separation via isocratic elution on a BEH C18 column. Mass spectrometric analysis was performed using electrospray ionisation in positive ionisation mode with multiple reaction monitoring for quantitative detection. The analytes demonstrated high separation efficiency, with a single sample run time of 3.0 min. The method exhibited a wide linear range with excellent linearity across the concentration range. The intra- and inter-batch precision were ≤10.00%, the accuracy was 88.67–113.29%. Accurate quantification of antipsychotics remained unaffected under various storage conditions: 72 h at room temperature, 7 d at 4 °C refrigeration, and 14 d at −80 °C freezing. This validated methodology has been successfully applied to plasma samples from patients with psychiatric disorders, demonstrating its practical utility for accurate quantification of antipsychotics in large-scale and complex matrices containing multiple analytes.
研究领域:
临床医学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Pharmaceutical Analysis
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2017
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IF 0
Jing Zeng,Hua lin Cai,Zhi ping Jiang,Qing Wang,Yan Zhu,Ping Xu,Xie lan Zhao
A sensitive, rapid, simple and economical ultra-performance liquid chromatography–tandem mass spectrometric method (UPLC–MS/MS) was developed and validated for simultaneous determination of imatinib, dasatinib and nilotinib in human plasma using gliquidone as internal standard (IS). Liquid-liquid extraction method with ethyl acetate was used for sample pre-treatment. The separation was performed on an Xtimate Phenyl column using isocratic mobile phase consisting of A (aqueous phase: 0.15% formic acid and 0.05% ammonium acetate) and B (organic phase: acetonitrile) (A:B=40:60, v/v). The flow rate was 0.25 mL/min and the total run time was 6 min. The multiple reaction monitoring (MRM) transitions, m/z 494.5→394.5 for imatinib, 488.7→401.5 for dasatinib, 530.7→289.5 for nilotinib and 528.5→403.4 for IS, were chosen to achieve high selectivity in the simultaneous analyses. The method exhibited great improvement in sensitivity and good linearity over the concentration range of 2.6–5250.0 ng/mL for imatinib, 2.0–490.0 ng/mL for dasatinib, and 2.4–4700.0 ng/mL for nilotinib. The method showed acceptable results on sensitivity, specificity, recovery, precision, accuracy and stability tests. This UPLC–MS/MS assay was successfully used for human plasma samples analysis and no significant differences were found in imatinib steady-state trough concentrations among the SLC22A5 −1889T>C or SLCO1B3 699G>A genotypes ( P >0.05). This validated method can provide support for clinical therapeutic drug monitoring and pharmacokinetic investigations of these three tyrosine kinase inhibitors (TKIs).
研究领域:
药理学
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临床医学
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分析化学
✓ 已验证
DOI
SENSORS AND ACTUATORS B-CHEMICAL
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2021
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IF 7.46
Zhengxing Song,Jing Wang,Jing Wang,Jin Liu,Xinzhi Wang,Yao Wang,Abdur Raheem Aleem,Matt J. Kipper,Laurence A. Belfiore,Jianguo Tang
Nanoparticle drug delivery systems are designed to be biocompatible and to control the distribution of poorly soluble drugs. Fluorescent nanoparticle drug carriers have been designed to enable fluorescence tracking. While such particles enable tracking of the carrier, this tracking does not report the local drug concentration. In this article, we report new nanoparticle drug carriers with stable, high-intensity fluorescence that sensitively reports the loaded drug concentration. The dumbbell-shaped nanoparticles are synthesized by combining europium (Eu) complexes with hyaluronic acid. The Eu complexes are superior to organic fluorophores, providing a narrow emission peak, high fluorescence intensity, long fluorescence lifetime, and insensitivity to photobleaching. The hyaluronic acid compatibilizes the nanoparticles with aqueous environments, and provides biocompatibility. We demonstrate that these polysaccharide nano-dumbbell aggregates (PNDAs) can effectively load drugs through structural changes, and sense the amount of drug loaded through changes in fluorescence intensity, enabling precise tracking of drug loading and release.
研究领域:
纳米技术
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药学
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生物医学工程
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材料科学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2025
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IF 5.1
Yu Tian,Ling Liu,Chaochao Ma,Yuhan Chu,Shuang Jiang,Shen Jiang,Guangrun Wu,Wenchao Wu,Chunjuan Yang,Yunpeng Wang,Ting Zhang,Yan Fang,Yang Li
The treatment of cardiovascular surgery relies on precise medication management at every stage. Blood contains a wealth of pharmacokinetic and pharmacodynamic information that provides an important basis for personalized drug delivery. Rapid and highly sensitive blood drug concentration assays can dynamically assess the distribution and metabolism of drugs in the body, optimize dose adjustment and improve therapeutic efficacy and safety. This study proposed a new method for real-time monitoring of cardiovascular drugs that combines the surface enhanced Raman spectroscopy (SERS) detection platform with a two-phase extraction strategy. First, silver nanoparticles were synthesized and modified with iodine ions, and calcium ions were further introduced to enhance the stability of the substrate and the Raman signal intensity. Subsequently, common interfering substances in plasma were removed by protein precipitation technology, and drug molecules were rapidly enriched by two-phase extraction to obtain a purer test sample. The experimental results showed that the method had good repeatability (RSD = 2.18 %), the minimum detection concentration of propranolol could reach 10 pg/mL, and the minimum detection limit of atropine was 500 pg/mL. In the SD rat model, the blood concentration of atropine reached a peak at about 2 h after oral administration. In addition, the animal model further demonstrated the ability of this method to accurately monitor the dynamic changes of blood drug concentrations. By comparing with the conventional detection results of UHPLC-MS/MS, the accuracy of this method in quantitative analysis was verified. This study not only expands the application potential of SERS technology in the detection of complex biological samples, but also highlights its broad prospects in drug analysis and clinical individualized treatment.
研究领域:
分析化学
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生物医学工程
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药理学
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纳米技术
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光谱学
✓ 已验证
产品关联
DOI
高影响因子
Materials Today
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2025
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IF 22
Muchao Chen,Yi Yu,Jiaying Hu,Yi-Chi Wang,Dongxu Zhao,Zhaoxin Ji,Lin Xu,Qiang Zhang,Baoye Ma,Yi Liu,Caifang Ni,Lin Gu,Youyong Li,Zhuang Liu,Yang Yang,Qian Chen
Hepatic arterial infusion chemotherapy (HAIC) delivers high local drug concentrations for hepatoma treatment but faces challenges such as catheter-related complications and systemic toxicity. To address these, we developed Mel-gel, an ultrasound-driven hydrogel system formed entirely by the chemotherapeutic agent Melphalan (Mel) without chemical modifications. Under ultrasound, Mel transitions from spherical to worm-like aggregates, rapidly forming a nanofiber-based hydrogel through non-covalent interactions. This innovative system offers sustained drug release, excellent bioavailability, and improved biosafety profiles. Mel-gel enables expedited catheter removal, reduces systemic toxicity, enhances local drug concentration, and prolongs drug-cell interaction time. Compared to conventional HAIC systems, Mel-gel’s unique in situ formation maximizes drug delivery and achieves superior targeting within complex vascular structures. In a rat orthotopic hepatoma model, Mel-gel-assisted HAIC demonstrated remarkable therapeutic efficacy, achieving a tumor inhibition rate of 98.09%, activating anti-tumor immune responses, and curing 60% of treated rats. This novel ultrasound-responsive hydrogel represents a promising advancement in hepatoma treatment, offering enhanced precision, efficacy, and safety. Additionally, Mel-gel’s versatile drug delivery capabilities may enhance the pharmacokinetics of various drugs and support synergistic therapies. This novel hydrogel system offers a promising advancement in hepatoma treatment, aiming to optimize outcomes and expand its application to other therapeutic agents.
研究领域:
肿瘤学
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药物递送
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纳米医学
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生物医学工程
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癌症治疗
✓ 已验证
产品关联
DOI
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
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2024
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IF 4.8
Jiaxin Zhang,Jiaqi Chen,Hui Wang,Chang Liu,Xin Yin,Haotian Bai,Mingwei Xu,Zuhao Li,Yanbing Wang,Xiujie Zhu,Xiaonan Wang,Ming Ren,Haowen Xue,Jincheng Wang,He Liu,Xin Zhao
Osteogenic–osteoclastic microenvironment imbalance impairs osseointegration between joint prostheses and the marrow cavity, which is a crucial contributor to the high incidence of complications such as implant loosening and periprosthetic fractures in osteoporosis (OPs). Previously, we employed hydrogel-based bisphosphonate (BPs) coatings on 3D-printed porous titanium alloy implants (pTi) to modulate the OPs microenvironment through controlled release. However, challenges persisted, including the limited duration of BPs release and associated organic material degradation-induced toxicity. To address these issues, we modified pTi surfaces by introducing chemically stable zeolites and BPs. In this study, we applied Ca 2+ -functionalized hierarchical zeolite coating on pTi for the first time. By combining mesoporous adsorption and Ca 2+ chelation, we achieved efficient encapsulation of zoledronate (ZOL), creating a bioactive interface characterized by a spatial gradient structure with a “microporous-mesoporous-macroporous” morphology. The coating achieves the release of ZOL through the mesoporous structure and facilitates the gradual release of chelated ZOL via ion exchange, resulting in hierarchical drug delivery. Moreover, the spatial gradient structure of the coatings significantly influenced cell extension. Simultaneously, we constructed a bioactive coating with a drug concentration gradient, achieving graded drug release within the microenvironment, which facilitated the accurate regulation of osteogenesis and bone resorption. This coating has the potential to substantially enhance the management of postoperative complications in OPs patients undergoing prosthetic replacement surgery by achieving a balanced osteogenic-osteoclastic response.
研究领域:
材料科学
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生物医学工程
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骨科
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药剂学
✓ 已验证
产品关联
DOI
高影响因子
BIOMEDICINE & PHARMACOTHERAPY
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2020
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IF 4.545
Song Chen,Yujie Ren,Peng Duan
Lung cancer still remains a leading cause of cancer mortality in the world. Obatoclax mesylate (OM), a B cell chronic lymphocytic leukemia/lymphoma 2 (Bcl-2) family antagonist, is a potential antitumor drug. However, its poor aqueous solubility restricts its clinical application. Although these inherent defects, nanotechnology can be used to improve the solubility and tumor target of OM, promoting its antitumor efficiency. In the present study, the poly(lactic-coglycolic acid) (PLGA) was used and combined with red blood-cell membrane (RBCm) to explore if OM-loaded RBCm nanoparticles could improve the antitumor efficacy of OM for the treatment of lung cancer with relatively lower side effects compared with the free OM. The good physicochemical stability of the prepared RBCm-OM/PLGA nanoparticles was confirmed, and the optimal size of 153 nm was screened out, along with sustained drug release behavior. We found that RBCm-OM/PLGA nanoparticles effectively reduced the proliferation of lung cancer cells. Additionally, RBCm-OM/PLGA nanoparticles considerably induced apoptosis in lung cancer cells by reducing Bcl-2 expression levels, accompanied with the improved Cyto-c releases in cytoplasm and Caspase-3 activation. Mitochondrial membrane potential was also obviously impaired in lung cancer cells incubated with RBCm-OM/PLGA nanoparticles. Compared with free OM, RBCm-OM/PLGA nanoparticles could greatly prolong the drug circulation time in vivo and upgraded the drug concentration accumulated in tumor tissue. Furthermore, RBCm-OM/PLGA nanoparticles exerted stronger antitumor efficacy in vivo against lung cancer progression with superior safety. Therefore, RBCm-OM/PLGA nanoparticles provided new potential for lung cancer therapy with the improved safety and therapeutic effect.
研究领域:
药理学
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肿瘤学
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纳米技术
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）