MICROCHEMICAL JOURNAL
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2025
·
IF 5.1
Zhenyu Zhang,Aoyu Ren,Yuchen Liu,Minzhen Yin,Yongjun Feng,Yaowu Liu,Huasheng Peng,Shanshan Chu
Paeonia lactiflora Pall. is an economically significant plant widely used in medicine and ornamentation, with a long history of cultivation. The prevalent cultivars of medicinal P. lactiflora in China display distinct morphological traits. Nevertheless, a systematic elucidation of their quality attributes remains to be achieved. This study employed ultra-performance liquid chromatography-quadrupole-orbitrap mass spectrometry (UPLC-Q-Orbitrap-MS) and ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-QQQ-MS/MS), combined with statistical analysis to investigate the metabolites of P. lactiflora roots in 17 medicinal cultivars from five major production regions in China. Significant morphological differences were observed in petal morphology, flower color, carpel features, as well as stem and leaf characteristics. In total, 167 compounds were identified or putatively characterized, including 101 phenolics, 36 terpenoids, 14 organic acids, eight amino acids, and eight other compounds (glycosides, polyols, lipids, and carbohydrates). The DD-SIMCA model established based on one-class classification successfully distinguished monopetalous red-flowered medicinal P. lactiflora from other types and differentiated herbs from Dao-di regions from those of non-Dao-di regions. Monopetalous red-flowered cultivars, representing an abundant germplasm resource, exhibited relatively high levels of terpenoids and phenolic compounds. This study has addressed the issue of compositional differences among different cultivars, screened out high-quality medicinal cultivars of P. lactiflora , and provided a theoretical basis for germplasm breeding work. Meanwhile, these findings offer a scientific rationale for the traditional view that MR cultivars are superior in traditional medicinal applications.
研究领域:
植物化学
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药用植物学
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中药学
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DOI
高影响因子
PLANT BIOSYSTEMS
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2024
·
IF 1.6
Krishna Pada Sahoo,Giyasuddin Siddique,Arindam Roy,Subhendu Ghosh,Mehedi Hasan Mandal
The study strives to investigate and document the traditional ethnomedicinal knowledge of indigenous plant species used by the tribal people of Jhargram district, West Bengal, India. It has been stated by the 142 informants that, out of a total of 80 plant species, 45 classes, 46 orders, 46 families and 75 genera are generally used as medicinal plants to cure several diseases. The most dominant medicinal plant families found in this region are Fabaceae, Apocynaceae, Anacardiaceae, Lamiaceae, Acanthaceae and Malvaceae. The most important species based on use value (UV) are Azadirachta excelsa, Cissus quadrangularis, Tylophora indica and Ocimum tenuiflorum. The informant consensus factor (Fic) values range from 0.46 to 0.71. The fidelity level (FL) value in the present study ranges from 25.58% to 93.42%. The study makes it clear that the aged informants have contributed more to the information about the value of the ethnomedicinal plants. Less information from the younger generation proves significant erosion of the traditional knowledge of this precious store. Some ethnomedicinal species with high UV and FL need to be reassessed for their phytochemical and pharmacological importance.
研究领域:
植物学
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民族药理学
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药用植物资源
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
·
IF 9.221
Qi Xiao,Zhenzeng Zhou,Zijie Shen,Jiandan Chen,Chunchuan Gu,Lihua Li,Fengnong Chen,Hongying Liu
Medicinal plants have been widely used in the treatment of various diseases for human health. We developed a novel method for the identification of closely related medicinal plants using a machine learning (ML)-based electrochemical fingerprinting platform. Firstly, the system featured a bare glassy carbon electrode capable of recording the voltammetric response of active components in medicinal plants as electrochemical fingerprints. Subsequently, different algorithms and various datasets were employed to analyze the correlation between the above electrochemical fingerprint data and the medicinal plant species . As a proof-of-concept, 6 species of Anoectochilus roxburghii ( A. roxburghii ) were selected as the verification samples. The electrochemical fingerprints of the samples were measured by differential pulse voltammetry in two buffer solutions. Thereafter, four powerful ML algorithms were utilized for the identification of A. roxburghii with different datasets. The results showed that the accuracy of identifying species reached 94.4 % by the nonlinear support vector machines based on the slope data of electrochemical responses in two buffer solutions, evidencing the successful discrimination of closely related medical plants by this method. Additionally, ML combined with electrochemical fingerprinting approaches had the advantages of being rapid, affordable, and straightforward, which provided potential applications in pharmaceutical research and plant taxonomy.
研究领域:
药用植物研究
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分析化学
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人工智能
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产品关联
DOI
高影响因子
Scientifica
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2020
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IF 0
Jasvidianto C. Kotta,Agatha B. S. Lestari,Damiana S. Candrasari,Maywan Hariono
Goat weed (Ageratum conyzoides L.), or bandotan in Indonesia, is an herbaceous plant that broadly grows up in both subtropical as well as tropical areas. This herb contains many phytoconstituents which have many benefits in different aspects. The essential oil contains phytochemicals such as phenol, phenolic ester, and coumarin, whereas many compounds can been identified in the whole part such as terpenoid, steroid, chromene, pyrrolizidine alkaloid, and flavonoid. Empirically, this herb has been used as an antihemorrhagic, antiseptic, antileprosy, and wound-healing agent. This article reviews the potency of the herb in medication according to the chemical substances being deposited, which are collected from numerous studies, followed by its in silico bioactivity prediction as well as its pharmaceutical dosage form formulation.
研究领域:
药理学
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药物化学
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植物学
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DOI
PubMed
Journal of the Iranian Chemical Society
·
2013
·Vol. 11
·期 2
·415-422
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IF 1.467
Zou Deqing,Qing Ye,Li Yingtang,Liu Mosheng,Yang Yaling
A highly sensitive method for the determination of manganese (VII), chromium (VI) and nickel (II) in medicinal herb samples is proposed. The method is based on analytes reacted with ammonium pyrrolidinedithiocarbamate (APDC) to give hydrophobic chelates (M–APDC), which were separated and enriched by cloud-point extraction (CPE) with non-ionic surfactant Tergitol TMN-6 as extractant. The surfactant-rich phase containing the chelates is determined with a high-performance liquid chromatography system. To achieve the best CPE method, the Box–Behnken design was used to study the concentration of Tergitol TMN-6, equilibrium temperature, equilibrium time as well as their interaction. What followed was the individual research for the pH of the sample solutions and the concentration of APDC. What is more, in the given optimized experimental conditions, calibration plots were found to be linear in the range of 0.0200–0.500 mg/L for Mn (VII) and Cr (VI), meanwhile 0.0500–1.00 mg/L for Ni (II), the linear correlation coefficients were between 0.996 and 0.999, the recoveries ranged from 91.8 to 97.8 % and the relative standard deviations were between 1.09 and 2.30 % ( n = 3). The limits of detection were 0.164 μg/L for Mn (VII), 0.562 μg/L for Cr (VI) and 5.12 μg/L for Ni (II), respectively. The proposed method was applied to determine manganese (VII), chromium (VI) and nickel (II) in medicinal herb samples with satisfactory results.
研究领域:
分析化学
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药学
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DOI
高影响因子
JOURNAL OF THE WORLD AQUACULTURE SOCIETY
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2022
·Vol. 53
·期 6
·1084-1100
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IF 3.402
Wei-Wei Mao,Zi-Chen Zhao,Gui-Hua Li,Wei-Liang Guo,Ze-Hao Ding,Shi-Feng Wang,Yun Sun,Zhen-Jie Cao,Jian-Long Li,Yong-Can Zhou
Cryptocaryon irritans , one of the most devastating holotrichous ciliated protozoans, causes massive economic losses in the pompano, Trachinotus ovatus , mariculture industries. Considering the many advantages of phytochemicals, such as low cost, low residuals, and low pollution, they can be a good alternative for the prevention and control of C. irritans infestations. In this study, 14 phytochemicals were evaluated for their antiparasitic activity against the theront of C. irritans , and nine showed significant antiparasitic activity. Nerolidol was the most promising compound, causing 100% mortality of theronts with 30-min exposure at a concentration of 10.0 μl/L. The half-maximal inhibitory concentrations ( IC 50 s) of nerolidol on C. irritans theronts, protomonts, and tomonts were 2.1, 13.3, and 7.1 μl/L, respectively. Abnormal morphologies of the theronts, trophonts, and tomonts were observed after exposure to 10.0, 30.0, and 10.0 μl/L nerolidol solution for 15 min, 30 min, and 480 min, respectively. A diet supplemented with 2.3 ml/kg of nerolidol reduced the number of trophonts on the gills of pompano by 95.7% decrease compared with the control groups and the theront chemotaxis to pompano mucus and sera, while increased the survival rate of infected pompanos by 24.44%, and had no significant effect on pompano hindgut and serum myeloperoxidase. These results suggest that nerolidol is a new potential drug for controlling fish cryptocaryonosis.
研究领域:
水产养殖学
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寄生虫学
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药理学
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2019
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IF 2.813
Yanwei Fu,Xiaowen Dou,Lei Zhang,Jiaan Qin,Meihua Yang,Jiaoyang Luo
Developing an analysis of multi-pesticide residues for different herbal species-ready applications is a challenge. In the present work, a comprehensive analysis was proposed for rapid detection of 201 pesticides in various medicinal herbs. Samples were extracted and cleaned up with a high throughput pretreatment approach (modified QuEChERS), and then detected by gas chromatograph coupled to an electron impact ionization triple quadrupole mass spectrometer (GC-EI-MS/MS). The clean-up procedure has been optimized using four types of representative medicinal herbs with different primary or secondary metabolites . Moreover, a mixture of analyte protectants (APs) was to improve the peak shape and intensity of some compounds. The performance of the method was validated according to the European Union SANTE/11813/2017 regulatory guidelines. The limit of quantification (LOQ) was determined to be ≤10 ng mL − 1 , and the recovery was between 70.0%–120.0%, with ≤20% RSD for the majority of pesticides. Sixty samples belonging to different species of medicinal herbs (such radix, flos, cortex, fructus, and seeds) were analyzed to evaluate the applicability of the optimized method. High frequency of chlorpyrifos was found in Citri reticulatae pericarpium, Crataegi fructus and Cuscutae semen samples.
研究领域:
分析化学
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植物学
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药物科学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2022
·
IF 5.304
Meichao Liao,Yang Zhao,Xiaoyue Yang,Lin Yang,E-Hu Liu,Bin Lu,Jiye Wang,Xiaopeng Liu,Yanzhong Chang,Li Duan
The green and efficient extraction of bioactive compounds from plant biomass is an extraordinary area of interest in the pharmaceutical industries. In a perspective of sustainable development, we investigate an eco-friendly route using recycle hydrophobic deep eutectic solvent-assisted water extraction (RHD-WE) to effectively and simultaneously extract five main phenolic acids (caftaric acid, chlorogenic acid, caffeic acid, cichoric acid, 3,5-di-O-caffeoylquinic acid) from medicinal plant. In the procedure, 20 mg Taraxacum mongolicum powder was mixed with 800 µL water and 200 µL camphor/p-chlorophenol-based hydrophobic deep eutectic solvent (DES) at a molar ratio of 1:3. After 10 min vortex and centrifugation, the mixture was separated into aqueous phase and DES phase. Compared with other published procedures, phenolic acids were enriched in the aqueous phase. Aqueous solution of phenolic acid could be directly used as raw materials without further purification in pharmaceutical industries. Besides, hydrophobic DES phase could easily be recovered and reused in recycling studies. This is the first study of the recyclability of hydrophobic DES. Generally speaking, the model for the extraction of phenolic acids was well established and verified. The extraction efficiency was higher than other traditional extraction methods, approximately 14 mg/g of phenolic acids were obtained in the aqueous phase. In addition, the extraction mechanism was preliminarily discussed in this research. Such novel RHD-WE method might improve the existing schemes for extraction of bioactive compounds from plant materials. This study suggested that hydrophobic DESs could be used as green and safe auxiliary extractant for pharmaceutical applications.
研究领域:
植物化学
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药物开发
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可持续发展
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产品关联
DOI
高影响因子
Analytical Methods
·
2021
·Vol. 14
·期 4
·401-409
·
IF 2.896
Yamei Han,Shan Wang,Yijie Liu,Ligai Bai,Hongyuan Yan,Haiyan Liu
Herein, a poly(ionic liquid@MOF) composite monolithic column was prepared via in situ radical polymerization using ionic liquid (1-allyl-3-methylimidazolium hexafluorophosphate) and MOF (derivatized UIO66-2COOH) as copolymer monomers. The composite monolithic column was characterized via scanning electron microscopy (SEM), nitrogen adsorption–desorption isotherms and mercury intrusion porosimetry. Subsequently, the composite monolithic column combined with high performance liquid chromatography (HPLC) was used as a solid-phase extraction (SPE) absorbent for online purification and enrichment of tectochrysin in medicinal plants. The results indicated that the addition of the ionic liquid and MOF not only increased the surface area but also increased the adsorption capacity of the monolith for tectochrysin. The method showed good linearity in the concentration range of 0.01–500 μg mL−1. The calibration equation was y = 2154.6x − 8.3785 and the limit of detection (LOD, S/N = 3) and the limit of quantification (LOQ, S/N = 10) were 3.33 ng mL−1 and 10 ng mL−1, respectively. The relative standard deviation (RSD) of the intra-day and inter-day precision was less than 2.62%, the RSD of inter-column was less than 3.16%, and the recoveries ranged from 100.58% to 105.00%. Thus, results showed that this method is simple, accurate and convenient for the online enrichment and purification of tectochrysin from medicinal plants.
研究领域:
分析化学
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药物分析
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材料科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2020
·Vol. 137
·期 36
·49081
·
IF 2.52
Huan Yu,Haiyan Liu,Xiaoya Pang,Miaomiao Zhang,Hongyuan Yan,Ligai Bai
In this study, a composite poly(functional graphene oxide- co - N -isopropylacrylamide- co -trimethylolpropane triacrylate- co -ethylene dimethacrylate) (poly(f-GO- co -NIPAAm- co -TMPTA- co -EDMA)) monolith was prepared, in which f-GO was added. The obtained monolith was characterized by nitrogen adsorption–desorption isotherm and scanning electron microscopy. The monolith exhibited a homogeneous and continuous structure, good permeability, and large surface area. The composite monolith was used as solid-phase extraction absorbent in conjunction with high-performance liquid chromatography for enrichment of ursolic acid. The calibration equation was y = 73.3980 x + 0.2555, and the linear regression coefficient was 0.9987; The calibration curve was ranged from 1 to 700 μg ml −1 ; The limit of detection and limit of quantification values were 0.3 and 1.0 μg ml −1 , respectively; The intraday and interday precisions were less than 2.6 and 7.4%, respectively. The recovery was in the range of 84.44–111.76%. Moreover, the adsorption amount of ursolic acid on the composite monolith was compared with the monolith without f-GO added and silica gel-C18 adsorbent. The results showed that the composite monolithic column was feasible to be used as an online SPE absorbent, which exhibiting low back pressure, good permeability, and high adsorption capacity. In addition, this method provided the possibility to extract and analyze trace components from medicinal plants.
研究领域:
分析化学
·
药学
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DOI
高影响因子
WORLD JOURNAL OF GASTROENTEROLOGY
·
2020
·
IF 3.665
Xiao Meng,Guo-Yi Tang,Pin-He Liu,Chan-Juan Zhao,Qing Liu,Hua-Bin Li
BACKGROUND Many natural products confer health benefits against diverse diseases through their antioxidant activities. Carbon tetrachloride (CCl 4 ) is often used in animal experiments to study the effects of substances on liver injury and the related mechanisms of action, among which oxidative stress is a major pathogenic factor. AIM To compare antioxidant and hepatoprotective activities of ten herbs and identify and quantify phytochemicals for the one with strongest hepatoprotection. METHODS The antioxidant activity of ten medicinal herbs was determined by both ferric-reducing antioxidant power and Trolox equivalent antioxidant capacity assays. The total phenolic and flavonoid contents were determined by Folin–Ciocalteu method and aluminum chloride colorimetry, respectively. Their effects on CCl 4 -induced oxidative liver injury were evaluated and compared in a mouse model by administrating each water extract (0.15 g/mL, 10 mL/kg) once per day for seven consecutive days and a dose of CCl 4 solution in olive oil (8%, v/v, 10 mL/kg). The herb with the strongest hepatoprotective performance was analyzed for the detailed bioactive components by using high-performance liquid chromatography-electrospray ionization source-ion trap tandem mass spectrometry. RESULTS The results revealed that all tested herbs attenuated CCl 4 -induced oxidative liver injury; each resulted in significant decreases in levels of serum alanine transaminase, aspartate transaminase, alkaline phosphatase, and triacylglycerols. In addition, most herbs restored hepatic superoxide dismutase and catalase activities, glutathione levels, and reduced malondialdehyde levels. Sanguisorba officinalis ( S. officinalis ) L., Coptis chinensis Franch., and Pueraria lobata (Willd.) Ohwi root were the three most effective herbs, and S. officinalis L. exhibited the strongest hepatoprotective effect. Nine active components were identified in S. officinalis L. Gallic acid and (+)-catechin were quantified (7.86 ± 0.45 mg/g and 8.19 ± 0.57 mg/g dried weight, respectively). Furthermore, the tested herbs displayed a range of in vitro antioxidant activities proportional to their phenolic content; the strongest activities were also found for S. officinalis L. CONCLUSION This study is of value to assist the selection of more effective natural products for direct consumption and the development of nutraceuticals or therapeutics to manage oxidative stress-related diseases.
研究领域:
药理学
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肝病学
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天然产物化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2015
·Vol. 407
·期 20
·6031-6039
·
IF 3.436
Li Dan,Zhu Qingxia,Lv Diya,Zheng Binxing,Liu Yanhua,Chai Yifeng,Lu Feng
By using a silver nanoparticle wiper as a surface-enhanced Raman scattering substrate, a highly sensitive, convenient, and rapid platform for detecting dye adulteration of medicinal herbs was obtained. Commercially available filter paper was functionalized with silver nanoparticles to transform it into the flexible wiper. This device was found to collect dye molecules with unprecedented ease. Experiments were performed to optimize various factors such as the type of wiper used, the wetting reagent, and the wetting/wiping mode and time. Excellent wiper performance was observed in the detection of the simulated adulteration of samples with dyes at various concentrations. The limits of detection for nine dyes, including 10 −6 g/mL for malachite green, 10 −7 g/mL for Rhodamine 6G, and 5 × 10 −8 g/mL for methylene blue, were discerned. The results of this investigation show that this proposed method is potentially highly advantageous for field-based applications.
Schematic diagram illustrating the fabrication of the paper-based SERS substrate, sample collection process on a herb and SERS examination with the portable Raman spectrometer
研究领域:
分析化学
·
药物分析
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）