MATERIALS & DESIGN
·
2023
·
IF 8.4
Yupeng Shi,Mengyang Zhou,Shuo Zhao,Haotian Li,Wenhao Wang,Jingliang Cheng,Lin Jin,Yifei Wang
Prevention of bacterial infection and control of inflammatory responses play critical roles in wound healing. Wound dressing loaded with antibacterial or anti-inflammatory drugs/molecules is one of the feasible routes in clinical applications. Nonetheless, effective and controllable drug release remains challenging. Herein, by electrospinning polyvinyl alcohol (PVA), polylactic acid-glycolic acid (PLGA) with copper sulfide nanoparticles (CuS NPs) and two model drugs, namely antibacterial drugs (mupirocin, M) and anti-inflammatory drugs (valsartan, V), an operable wound-healing patch consist of amphiphilic Janus nanofiber membrane (PLGAV-CuS/PVAM) was prepared which was used to controllable gradient drug release in wound healing. Janus nanofiber membrane shows typical amphiphilic characteristics, with low biological toxicity and good biocompatibility. In vitro experiments showed that the nanofiber membrane could slowly and continuously release mupirocin in a hydrophilic environment for antibacterial purposes. In addition, the excellent photothermal effect of PLGAV CuS/PVAM can regulate the release rate of the anti-inflammatory drug valsartan, effectively reduce the inflammatory reaction in the wound healing process, and also show enhanced bacteriostasis efficiency (96.3% of Escherichia coli and 97.8% of Staphylococcus aureus). The prepared patches exhibited excellent antibacterial, anti-inflammatory, and sustained-release effects, which is of great significance for board the avenue of nanofiber in the biomedical field.
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高影响因子
Materials Today Bio
·
2023
·
IF 8.2
Ming-Chen Sun,Ying-Fang Chen,Di Liu,Xiao-Ling Xu,Yu-Chan You,Wei Lu,Yun-Juan Shi,Ming-Yang Ren,Yi-Bin Fan,Yong-Zhong Du,Xiao-Hua Tao
The widespread utilization of mupirocin to treat methicillin-resistant Staphylococcus aureus (MRSA)-caused infectious diseases has led to the emergence of mupirocin-resistant Staphylococcus aureus (MuRSA), posing a serious global medical threat. In order to counteract MuRSA, we develop a d -α-tocopherol polyethylene glycol 1000 succinate (TPGS) modified mupirocin and silver complex (TPGS/Mup-Ag) to combat MuRSA. The surfactivity of TPGS endows Mup-Ag with a homogeneous and small particle size (∼16 nm), which significantly enhances bacterial internalization. Silver ions are released from the mupirocin-Ag complex (Mup-Ag) to exert a synergistic antibacterial activity with mupirocin. Results manifest that our strategy reduces the concentration of mupirocin that induces 50% bacterial death from about 1000 μmol/mL to about 16 μmol/mL. In vitro bacterial infection model suggests that TPGS/Mup-Ag can not only eliminate both intracellular and inhibit bacterial adhesion, but also living cells are not affected. Results of in vivo experiments demonstrate that TPGS/Mup-Ag can effectively inhibit the progression of skin infection and accelerate wound healing, as well as alleviate systemic inflammation in both the subcutaneous infection model and the wound infection model. Furthermore, this study may contribute to the development of therapeutic agents for antibiotic-resistant bacteria and offer ideas for silver-based bactericides.
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PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 14
·4613
·
IF 4.927
Haohua Liu,Yue Cai,Yuteng Chu,Xiaojie Yu,Fuhang Song,Hong Wang,Huawei Zhang,Xuanrong Sun
Chrysomycin A, a compound derived from marine microorganisms, proved to have a specific great in vitro inhibitory effect on methicillin-resistantStaphylococcus aureus(MRSA). It exhibits high safety for the skin, as well as a better therapeutic effect than the current clinical drug, vancomycin. Nevertheless, its poor water solubility highly limits the application and reduces the bioavailability. In view of this, we developed a cream of chrysomycin A (CA) to enhance the solubility for the treatment of skin infection, while avoiding the possible toxicity caused by systemic administration. A comprehensive orthogonal evaluation system composed of appearance, spreading ability, and stability was established to find the optimal formula under experimental conditions. The final product was odorless and easy to be spread, with a lustrous, smooth surface. The particle size of the product met Chinese Pharmacopoeia specifications and the entire cream showed long-term stability in destructive tests. The in vitro and in vivo studies indicated that CA cream had a similar anti-MRSA activity to commercially available mupirocin, showing its potential as an efficacious topical delivery system for skin infections treatment.Keywords:MRSA;chrysomycin A;cream;topical administration;skin infections
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高影响因子
Nature Communications
·
2019
·Vol. 10
·期 1
·1-15
·
IF 11.878
Li Jun,Liu Xiangmei,Tan Lei,Cui Zhenduo,Yang Xianjin,Liang Yanqin,Li Zhaoyang,Zhu Shengli,Zheng Yufeng,Yeung Kelvin Wai Kwok,Wang Xianbao,Wu Shuilin
The application of photothermal therapy to treat bacterial infections remains a challenge, as the high temperatures required for bacterial elimination can damage healthy tissues. Here, we develop an exogenous antibacterial agent consisting of zinc-doped Prussian blue (ZnPB) that kills methicillin-resistant Staphylococcus aureus in vitro and in a rat model of cutaneous wound infection. Local heat triggered by the photothermal effect accelerates the release and penetration of ions into the bacteria, resulting in alteration of intracellular metabolic pathways and bacterial killing without systemic toxicity. ZnPB treatment leads to the upregulation of genes involved in tissue remodeling, promotes collagen deposition and enhances wound repair. The efficient photothermal conversion of ZnPB allows the use of relatively few doses and low laser flux, making the platform a potential alternative to current antibiotic therapies against bacterial wound infections.
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2024
·
IF 5.3
Yue Cai,Xinrui Zhang,Wentao Hu,Fuhang Song,Hong Wang,Huawei Zhang,Xuanrong Sun
Transdermal drug delivery represents a promising avenue for the treatment of dermatologic diseases, such as cutaneous melanoma and skin infections. This study involves the development of a novel therapeutic strategy that employs a skin-penetrating peptide SPACE-modified flexible liposomal chrysomycin A (CA@SPACE-LP) with a particle size of 111.5 nm. In vitro transdermal experiments demonstrated that CA@SPACE-LP was effective in enhancing the ability of the drug to penetrate the stratum corneum and enter deep into the skin tissue, increasing the intradermal drug concentration up to threefold compared to free CA. Furthermore, CA@SPACE-LP was observed to maintain the biological activity of CA against planktonic Methicillin-resistant Staphylococcus aureus (MRSA) and melanoma cells. In vivo studies demonstrated that the topical administration of CA@SPACE-LP was efficacious in controlling the progression of cutaneous melanoma, with a tumor suppression rate of approximately 60 %, which was more pronounced than that observed with intravenous Taxol. Furthermore, CA@SPACE-LP demonstrated efficacy in the management of intradermal MRSA infections, with a significantly reduced area of ulceration in the treated mice (0.25 cm 2 ) compared to the positive control drug (Mupirocin Ointment). These results suggest that the topical delivery system developed in this study has the potential to be used for the simultaneous treatment of skin cancer and invasive MRSA infection.
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Chunyang Li,Jin-Tao Wang,Kuili Liu,Hongwei Ding,Qing-Feng Li,Gaofeng Liang,Lin Jin,Deyan He
Balancing short-term antibacterial and long-term anti-inflammation is a great challenge during wound healing. To resolve this problem, a double-layer (DL) hydrogel was designed to achieve the sequential release of drugs. The lower layer was hyaluronic acid (HA) loaded with mupirocin (MUP), and the upper layer was gelatin-N-[tris(hydroxymethyl)methyl] acrylamide hybrid (GTH) hydrogel loaded with ibuprofen (IBU) and MXene nanosheets. The lower layer was applied to the wound site and HA gradually degraded to achieve efficient antibacterial. Simultaneously, MXene was used as a photothermal agent and dispersed in the upper hydrogel, allowing the upper layer to effectively achieve controlled drug release and implement anti-inflammation through the photothermal effect. The in vivo experiment results showed that the prepared DL hydrogel had excellent antibacterial and anti-inflammatory effects, and could effectively promote the repair and regeneration of bacteria contaminated wound tissue. It has been shown that the DL hydrogel has great application prospects in the field of wound treatment.
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高影响因子
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