LWT-FOOD SCIENCE AND TECHNOLOGY
·
2022
·
IF 6.056
Qiu-Lan Liu,Yang Yi,Shi-Qi Wang,Hong-Xun Wang,Wei Xu,Ting Min,Li-Mei Wang
Lotus root is the most consumed aquatic vegetable in China. Both the actual boiling of lotus root and the simulated boiling of its extract were performed at different conditions to explore the development of non-enzymatic browning and the loss of reducing sugars, free amino acids, total free phenols and ascorbic acid. The kinetic parameters of zero-order and first-order models indicated that both browning degree and nutrient loss had a positive correlation with boiling time, temperature and pH value. Moreover, nutrient contents and boiling conditions were adopted as independent variables to establish the multiple linear regression model of browning degree ( P < 0.001), which confirmed that reducing sugars and total phenols principally contributed to the browning ( P < 0.01), through caramelization and phenol oxidation. With the addition of baking soda (pH 8), 100 °C boiling decreased the DPPH radical scavenging capacity and ferric reducing antioxidant power of lotus root extract by 62.96% and 58.51%, respectively. Furthermore, 120 °C boiling probably induced the formation of browning products, which possessed cytotoxicity against HepG2 and L02 cells. A short boiling time, as well as an acidic condition induced by added vinegar, is recommended for cooking and processing of lotus root in consideration of nutrition and health.
研究领域:
食品科学
·
植物学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2023
·Vol. 12
·期 5
·1049
·
IF 5.2
Qiulan Liu,Xiaoqin Zou,Yang Yi,Ying Sun,Hongxun Wang,Xueyu Jiang,Kaidi Peng
To promote the functional applications of lotus root polysaccharides (LRPs), the effects of noncovalent polyphenol binding on their physicochemical properties, as well as antioxidant and immunomodulatory activities, were investigated. Ferulic acid (FA) and chlorogenic acid (CHA) were spontaneously bound to the LRP to prepare the complexes LRP-FA1, LRP-FA2, LRP-FA3, LRP-CHA1, LRP-CHA2and LRP-CHA3, and their mass ratios of polyphenol to LRP were, respectively, 121.57, 61.18, 34.79, 2359.58, 1276.71 and 545.08 mg/g. Using the physical mixture of the LRP and polyphenols as a control, the noncovalent interaction between them in the complexes was confirmed by ultraviolet and Fourier-transform infrared spectroscopy. The interaction increased their average molecular weights by 1.11~2.27 times compared to the LRP. The polyphenols enhanced the antioxidant capacity and macrophage-stimulating activity of the LRP depending on their binding amount. Particularly, the DPPH radical scavenging activity and FRAP antioxidant ability were positively related to the FA binding amount but negatively related to the CHA binding amount. The NO production of the macrophages stimulated by the LRP was inhibited by the co-incubation with free polyphenols; however, the inhibition was eliminated by the noncovalent binding. The complexes could stimulate the NO production and tumor necrosis factor-α secretion more effectively than the LRP. The noncovalent binding of polyphenols may be an innovative strategy for the structural and functional modification of natural polysaccharides.Keywords:lotus root;polysaccharide;polyphenol;noncovalent interaction;bioactivity
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yanbei Zhang,Jie Li,Shoulei Yan
Lactic acid bacteria fermentation significantly enhances the color quality of fruit and vegetable juices and delays non-enzymatic browning. Nevertheless, the underlying mechanisms had rarely been reported and the existing ones were only relatively superficial studies from the perspective of browning substrates. Therefore, the mechanism of lactic acid bacteria fermentation mitigating the browning of lotus rhizome juice was investigated from the perspective of browning product removal in this research. Results showed lactic acid bacteria improved the color of lotus rhizome juice by adsorbing browning pigment with 53.42 %. Peptidoglycan was the key site for pigment adsorption, with this capability being intricately linked to its composition and structure. Electrostatic and hydrophobic forces were involved in browning pigment adsorption. Notably, cell viability was not a factor that affected pigment adsorption. In the future, inactivated lactic acid bacteria can be widely used as biosorbents to enhance the color of unfermented food.
研究领域:
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Chengcheng Zhai,Mingwei Wang,Yanke Lu,Hongyuan Yan
Polycyclic aromatic hydrocarbons (PAHs) posed a serious threat to food safety and human health due to long-term emission. In this work, a new method was established using phloroglucinol-urotropine porous polymer (PU-PP) in a pipette tip for solid phase extraction (PT-SPE) for the first time and used prior to determination of four PAHs (phenanthrene, anthracene, fluoranthene, and pyrene) in lotus roots. Synthesis of the PU-PP adsorbent was green compared with alternatives; urotropine was used as a cross-linker and ethanol–water as the solvent. PU-PP-based PT-SPE had the advantages of low solvent consumption, good purification, practicability, stability, and low-cost. The proposed pre-purification method offered low limits of detection (0.09–0.28 ng/g) and good recoveries (84.6–114.3 %, RSDs ≤ 5.6 %) for determination of the four PAHs, which were detected at trace concentrations in samples. This new method provides an alternative for monitoring trace pollutants in aquatic plant ingredients.
研究领域:
食品安全
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环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yan Zhang,Jiangfeng Song,Caie Wu,Guozhe Deng
The study aimed to construct an effective composite of lutein-loaded lotus root starch nanoparticles (LUT-LRSNPs) and evaluate its in vitro anti-inflammatory activity. LUT-LRSNPs were prepared via the antisolvent nanoprecipitation method and characterized by infrared spectroscopy, X-ray diffraction, and iodine complexation. The result confirmed that lutein was successfully encapsulated in LRSNPs with an encapsulation efficiency up to 84.6 %. Lutein combined with LRSNPs through hydrogen bonding and hydrophobic interaction to form a stable composite, which enhanced the photothermal stability of lutein and realized its controlled release. The cumulative release of lutein in simulated intestinal fluid reached 86.5 %. An inflammatory model was established by stimulating Caco-2 cells with lipopolysaccharide. LUT-LRSNPs significantly decreased the intensity of ROS, inhibited the secretion of TLR4 and the phosphorylation of p38, thus reducing the activation of the NF-κB pathway. It also reduced NO content, IL-1β, IL-6, and TNF-α secretion, as well as the corresponding mRNA expressions in a concentration-dependent manner, with the effect significantly superior to lutein. The prevention group generally exhibited better inhibitory effect than that of the treatment group. In conclusion, LUT-LRSNPs emphasized the enhanced anti-inflammatory effects of lutein and could constitute an alternative for preventing or alleviating related inflammations.
研究领域:
食品科学
·
生物医学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2019
·
IF 4.916
Danyu Zhang,Shiwei Yang,Heyong Cheng,Yuanchao Wang,Jinhua Liu
Multi-elemental speciation analysis in a single run reduces costs, waste and time, and also sample consumption, which is favorable to a large set of instable samples. This work demonstrates mercury and arsenic speciation in a single run using high performance liquid chromatography with inductively coupled plasma mass spectrometric detection (HPLC/ICP-MS). Thiol compounds in mobile phases interfered with the elution of arsenite because of coordination action, which was overcome by gradient elution (elution of As(III) by thiol-free mobile phases at first). Four arsenic forms (As(III), DMA , MMA and As(V)) and three mercury species (Hg(II), MeHg and EtHg) were separated and detected by HPLC/ICP-MS using 4 mM tetrabutylammonium hydroxide (TBAH) (pH 6.0) in the first 1.0 min and 4 mM TBAH + 20 mM L -cysteine (pH 6.0) in the next 9.0 min. The detection limits were obtained at 0.078 for As(III), 0.081 for DMA, 0.078 for MMA and 0.15 μg L −1 for As(V), and 0.016 for Hg(II), 0.027 for MeHg and 0.032 μg L −1 for EtHg, and the reproducibilities (50 μg L −1 for each As-species and 10 μg L −1 for each Hg-species) were all lower than 3.7%. The proposed method was successfully applied for speciation analyses of arsenic and mercury in five lotus roots, which indicated DMA (3.5–7.9 μg kg −1 ) as the only arsenic species and MeHg (0.6–1.0 μg kg −1 ) as the only mercury species. Analyses of two certified reference materials demonstrated good agreement between determined values and certified values. All results prove its advantages including high sensitivity and high analytical efficiency, which are beneficial for routine speciation analyses of arsenic and mercury in a single run in environmental and food fields.
研究领域:
环境科学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Jie Song,Zhao Zhang,Hongxun Wang,Yan Meng,Ying Sun,Yang Yi
Lotus root is a widely popular aquatic vegetable with edible and medicinal values. Here we report the structure and lipid-lowering activity of two lotus root polysaccharides LRW (lotus root polysaccharide by water extraction) and LRA (lotus root polysaccharide by alkali extraction), that were extracted by aqueous and alkaline solution respectively. The results showed that the yield of polysaccharide from lotus root could be significantly improved by alkali extraction. Basic composition and structural characterization showed that the total sugar contents of LRW and LRA were 96.83 % and 73.66 %, and the molecular weights were 2.464 × 10 5 Da and 1.727 × 10 5 Da, respectively. LRW and LRA had the similar structure that the main backbone consisted of →4)-α-D-Glcp-(1→ with branches at C-6 site. Both LRW and LRA could scavenge DPPH and hydroxyl radicals effectively, and have strong adsorption capacity to cholate salts in a concentration-dependent manner. In HepG2 cells, LRW and LRA inhibited the accumulation of lipid droplets induced by oleic acid, and increased the activity of T-SOD and CAT, meanwhile, reduced the level of MDA, TC and TG, showing good lipid-lowering activity. In comparison, the lipid-lowering effect of LRA was better than that of LRW. In addition, gene sequencing and RT-PCR showed that AMPK, ACC, PPARα and CPT-1 were essential for LRA to exert a lipid-lowering effect. This study provides a theoretical basis for the extraction and lipid-lowering application of lotus root polysaccharides.
研究领域:
食品科学
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营养学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Science China-Chemistry
·
2015
·Vol. 58
·期 5
·858-862
·
IF 1.695
Tang Jia,Zhou Bin,Zhang Shilin,Wang Zhuang,Xiong Lei,Li Peng
Unique lotus-rootlike Au-ZnO hybrid structures were obtained by controlling the deposition of pre-synthesized Au nanocrystals onto the surfaces of as-obtained ZnO structures. ZnO with lotus-rootlike structures was first prepared through a hydrothermal process. We also investigated the effects of various Au contents on the photocatalytic activities in detail. Notably, compared to the pure ZnO component, these resulting lotus-root-like Au-ZnO nanostructures with the appropriate amounts of Au content exhibited better photocatalytic efficiency.
研究领域:
材料科学
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纳米技术
·
光催化
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 30
·16574-16584
·
IF 2.936
Hong-Xun Wang,Yang Yi,Jie Sun,Olusola Lamikanra,Ting Min
Thirty-nine polysaccharides isolated from different parts of 13 lotus root varieties were characterized with fingerprint and chemometrics analyses to explore their similarity and diversity. The physicochemical features of lotus root polysaccharides (LRPs) were found to be the following: LRPs contained mainly polysaccharides (5.94 kDa) and polysaccharide-protein complexes (11.57 kDa and 5.30 kDa); their carbohydrates were composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose approximately in the molar ratio of 0.19 : 0.14 : 0.08 : 0.17 : 6.49 : 1.00 : 0.16; and node LRPs possessed more binding proteins and uronic acids than both flesh and peel LRPs. Their fingerprints based on Fourier-transform infrared spectroscopy, pre-column derivatization high-performance liquid chromatography and high performance size-exclusion chromatography all exhibited relatively high similarities, contributing to the common figerprint models which could be utilized as references for the identification of LPRs. In addition, the fingerprint characteristics associated with the between-group variability of LRPs in the score plots derived from multivariate analytical models might indicate which variety or part of lotus root they were isolated from. Therefore, multi-fingerprinting techniques have the potential to be applied to the identification and quality control of LRPs.
研究领域:
植物化学
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食品科学
·
中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Ying-Ying Chen,Kang Liu,Xue-Qiang Zha,Qiang-Ming Li,Li-Hua Pan,Jian-Ping Luo
In this study, luteolin-oxidized lotus root starch (OLRS) nanoparticles (NPs) were developed to improve the stability and antioxidant activity of luteolin . Results showed that a stable luteolin-OLRS NPs was formed using luteolin and OLRS (oxidation degree, 15%) in the weight ratio of 3:1, as well as anti-solvent and solvent in the volume ratio of 10:1. Under this condition, the particle size, polydispersity index and zeta-potential of luteolin-OLRS NPs was 305 nm, 0.173 and −20.8 mV, respectively. The analysis of transmission electron microscopy , X-ray diffractometer and Fourier transform infrared spectroscopy demonstrated that the luteolin was successfully encapsulated in OLRS NPs, giving an encapsulation efficiency of 87.2%. The release characteristic and antioxidant activity of encapsulated luteolin were further investigated. Results exhibited that the OLRS NPs enabled luteolin to be stable in simulated gastric fluid and sustained release in simulated intestinal fluid, leading to the enhancement of antioxidant activity of luteolin.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yanke Lu,Pengfei Li,Liuliu Cheng,Lianfeng Ai,Jiankun Cao,Hongyuan Yan
Halogenated carbazoles (HCZs) are prevalent in sediments, pose a risk of entering the human food chain via lotus roots. However, starch, protein, and fat in lotus root complicate determination of HCZs in this food. This study introduces a straightforward, sensitive, and rapid method for detecting four HCZs in lotus roots. The method employs a conical monolithic extraction column (MEC) containing chitosan-glutaraldehyde-graphene oxide aerogel (CS-GA-GOA) material. The CS-GA-GOA − MEC successfully achieved extraction of four HCZs in spiked lotus root samples in under 5 min. The method was rapid (15 min) compared with alternatives, as well as being sensitivity (0.08–0.27 ng/g), accurate (89.7 %–104.2 %), and more cost-effective. This work contributes to the exploration of advanced adsorbents but also broadens the applicability of graphene oxide aerogels in extraction processes, paving the way for the development of rapid and accurate analytical methods for HCZs in foods.
研究领域:
环境科学
·
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2022
·Vol. 33
·期 14
·11446-11454
·
IF 2.779
Lu Youluan,Mu Xinwei,Liu Yaopeng,Shi Zhen,Zheng Yin,Huang Wensheng,Lou Fangming
Through simple bioconcentration, salt molecules of NaCl and KCl were introduced into lotus root, then a class of porous carbon materials (CPLR) with 3D pore structure based on the as-prepared lotus root was prepared by direct pyrolysis under different temperature. The CPLR was characterised by scanning electron microscopy, transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, and Raman spectra (Raman). In addition, the electrochemical performance of the CPLR-based sensors was examined by electrochemical impedance spectroscopy and cyclic voltammetry. Under the first-rank experimental conditions, the response current increased linearly with the concentration of hesperetin in the range from 0.05 to 100.0 μmol/L, and the minimum detectability was calculated to be 0.011 μmol/L ( S / N = 3). The sensor in Chinese herbal medicine buddleja officinalis maxim and healthy human urine samples performed well.
研究领域:
材料科学
·
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）