Linna Guo,Miao Tian,Zhi Li,Qianqian Wang,Qiuhua Wu,Lin Hao,Chun Wang
Wide use of phenylurea herbicide has caused serious residue problem and threaten human health. It is important to develop viable method for their sensitive determination. Herein, a multi-functionalized porous polymer was prepared by crosslinking hexafluorobisphenol A with pyromellitic dianhydride. Using the multi-functionalized porous polymer as solid phase extraction sorbent, combined with high performance liquid chromatography, a sensitive method was established for determining phenylurea herbicides in beverages and celtuces. High sensitivity was achieved, with method detection limit (S/N = 3) of 0.01–––0.025 ng mL −1 for beverages, 1.70 ng g −1 for celtuce, and quantitation limits of 0.03–––0.10 ng mL −1 for beverages, 5.00 ng g −1 for celtuce. The method recoveries were 80.5–––120.0 % with relative standard deviations lower than 6.1%. Adsorption mechanism mainly involved F-π, F-O, π-π, polar interactions and hydrogen-bonding interactions. This study offers a simple protocol to develop multi-functional sorbents for extraction of organic pollutants.
The present research assessed the effects of lettuce glycoside B (LGB), a compound separated and purified from Pterocypsela
laciniata , on irradiation-induced pulmonary fibrosis and explored the mechanism involved. Animal model of irradiation exposure inducing pulmonary fibrosis was established by Co irradiator. Rats were intraperitoneally treated with LGB (100, 200 and 400 mg/kg) once per day for a month. Lung index data were analyzed. The levels of fibrosis were assessed by hydroxyproline (Hyp) of pulmonary and lung tissue sections after irradiation exposure. Alveolitis and fibrosis levels were calculated from semi-quantitative analysis of hematoxylin and eosin and Masson’s trichrome lung section staining. The serum levels of transforming growth factor β1 (TGF-β1), interleukin (IL)-6, and tumor necrosis factor-α (TNF-α) were also evaluated. Antioxidant enzymes of superoxide dismutase (SOD) were measured in serum. Moreover, we also measured serum malondialdehyde (MDA) levels, a marker of oxidative stress. Treatment with LGB significantly reduced mortality rates and lung index scores and MDA content, enhanced SOD and other antioxidant enzymes activity, and regulated serum levels of TGF-β1, IL-6, and TNF-α. These results demonstrated that LGB significantly inhibited irradiation-induced pulmonary fibrosis. Furthermore, the results suggested promising clinical effect of LGB therapies for treating irradiation-induced pulmonary fibrosis.
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